• 한방비만학회지
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• 제18권1호
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• pp.10-18
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• 2018

Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel. Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2$/10% $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A. Results: Dangkwisoo-san extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration $(IC)_{50}$ of Dangkwisoo-san was $218.3{\mu}g/mL$. Also, ginger extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the $IC_{50}$ of ginger was $877.2{\mu}g/mL$. However, curcumin had no effects on TRPM7 whole-cell currents. Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.

• 한국미생물·생명공학회지
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• 제49권2호
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• pp.148-156
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• 2021

Single-chain antibodies against epidermal growth factor receptor variant III (EGFRvIII) are potentially promising agents for developing antibody-based cancer treatment strategies. We described in our previous study the successful expression of an anti-EGFRvIII scFv antibody in Escherichia coli. However, we could also observe the formation of insoluble aggregates in the periplasmic space, limiting the production yield of the active product. In the present study, we investigated the mechanisms by which growth conditions could affect the expression of the soluble anti-EGFRvIII scFv antibody in small-scale E. coli NiCo21(DE3) cultures, attempting to maximize production. The secreted scFv molecules were purified using Ni-NTA magnetic beads and protein characterization was performed using SDS-PAGE and western blot analyses. We used the ImageJ software for protein quantification and determined the antigen-binding activity of the scFv antibody against the EGFRvIII protein. Our results showed that the highest percentage of soluble scFv expression could be achieved under culture conditions that combined low IPTG concentration (0.1 mM), low growth temperature (18℃), and large culture dish surface area. We found moderate-yield soluble scFv production in the culture medium after lactose-mediated induction, which was also beneficial for downstream protein processing. These findings were confirmed by conducting western blot analysis, indicating that the soluble, approximately 30-kDa scFv molecule was localized in the periplasm and the extracellular space. Moreover, the antigen-binding assay confirmed the scFv affinity against the EGFRvIII antigen. In conclusion, our study reveals that low-speed protein expression is preferable to obtain more soluble anti-EGFRvIII scFv protein in an E. coli expression system.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권1호
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• pp.406-434
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• 2019

In general, an information security approach estimates the risk, where the risk is to occur due to an unusual event, and the associated consequences for cloud organization. Information Security and Risk Management (ISRA) practices vary among cloud organizations and disciplines. There are several approaches to compare existing risk management methods for cloud organizations but their scope is limited considering stereo type criteria, rather than developing an agent based task that considers all aspects of the associated risk. It is the lack of considering all existing renowned risk management frameworks, their proper comparison, and agent techniques that motivates this research. This paper proposes Agent Based Information Security Framework for Hybrid Cloud Computing as an all-inclusive method including cloud related methods to review and compare existing different renowned methods for cloud computing risk issues and by adding new tasks from surveyed methods. The concepts of software agent and intelligent agent have been introduced that fetch/collect accurate information used in framework and to develop a decision system that facilitates the organization to take decision against threat agent on the basis of information provided by the security agents. The scope of this research primarily considers risk assessment methods that focus on assets, potential threats, vulnerabilities and their associated measures to calculate consequences. After in-depth comparison of renowned ISRA methods with ABISF, we have found that ISO/IEC 27005:2011 is the most appropriate approach among existing ISRA methods. The proposed framework was implemented using fuzzy inference system based upon fuzzy set theory, and MATLAB(R) fuzzy logic rules were used to test the framework. The fuzzy results confirm that proposed framework could be used for information security in cloud computing environment.

• 한방비만학회지
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• 제18권2호
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• pp.57-63
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• 2018

Objectives: Metabolic syndrome is defined by a cluster of major cardiovascular risk factors: obesity, insulin resistance, dyslipidemia, and arterial hypertension. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential vanilloid 4 (TRPV4) channels have been associated with the development of dyslipidemia and hypertension. The purpose of this study was to investigate the effects of Leejung-tang (Lizhong-tang), Rikkunshito, and Bojungikgi-tang (Buzhongyiqi-tang) on TRPV4 channel. Methods: Human embryonic kidney 293 cells stably transfected with the TRPV4 expression vectors were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2/10%$ $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software (Molecular Devices, San Jose, CA, USA), and signals were digitalized at 5 kHz using Digidata 1422A. Results: Leejung-tang and Rikkunshito (10, 30 and 50 mg/mL) had no effects on the TRPV4 whole-cell currents at dose dependent manner. However, Bojungikgi-tang (10, 30, and 50 mg/mL) inhibited the TRPV4 whole-cell currents in a dose dependent manner and the half maximal inhibitory concentration (IC50) of Bojungikgi-tang was 18.2 mg/mL. Conclusions: These results suggest that Bojungikgi-tang plays an important roles to inhibit the TRPV4 channel, suggesting that Bojungikgi-tang is considered one of the candidate agents for the treatment of metabolic syndrome such as dyslipidemia and hypertension.

• 정보보호학회논문지
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• 제18권4호
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• pp.153-166
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• 2008

현재 인터넷의 발달과 봇의 사용이 활발해짐에 따라 컴퓨터와 사람을 분류할 수 있는 수단인 캡차(CAPTCHA)가 많이 활용되고 있다. 글자를 이미지 형태로 출력한 후 이를 변형시키는 방법이 많이 사용되는 캡차는 많은 연구 활동에 의하여, 인공지능 기법을 사용하면 쉽게 무력화 될 수 있음이 알려져 있다. 이에 대한 대안으로 이미지를 활용하는 캡차가 주목받고 있고 그에 따라 여러 형태의 이미지 기반 캡차가 제안 및 구현되었다. 그러나 이미지를 활용하는 캡차 또한 각각의 여러 다른 문제가 있는 것도 사실이다. 이 논문에서는 이러한 문제점에 대해서 짚어보고 이를 해결하기 위한 방안으로 복수의 이미지를 합성하는 캡차를 제안하였다. 또한 안전한 캡차의 운용을 위하여 가상 세션을 사용하지 않는 통신 프로토콜을 제안하였으며, 이에 따른 세부 사항에 대해서 논의하였다.

• 정보보호학회논문지
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• 제19권6호
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• pp.105-112
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• 2009

오늘날 캡차(CAPTCHA)는 계정 생성, 광고, 스팸 메일 등 자동화된 소프트웨어 대리자에 의한 다양한 공격을 방어하는데 널리 사용되고 있다. 초기 캡차의 문자들은 왜곡이 심하지 않아서 사용자들은 캡차 문자들을 쉽게 인식할 수 있었다. 이런 이유 때문에 이미지 처리, 인공지능 등의 다양한 기술들을 사용하여 많은 캡차들이 쉽게 무력화 되었다. 이에 대한 대안으로 캡차에 노이즈를 추가하거나 캡차 문자를 왜곡함으로써 문자 기반의 캡차 공격을 어렵게 만들었지만 캡차에 노이즈를 추가하거나 캡차 문자를 왜곡하는 것은 사용자들이 캡차 문자를 읽는 것을 더 어렵게 만들었다. 캡차의 가독성을 보완하기 위하여 몇몇 캡차들은 서로 다른 색상을 가진 문자를 사용했다. 그러나 서로 다른 문자의 색상을 사용하는 것은 캡차를 공격하기 원하는 공격자에게 이점을 제공했다. 이 논문에서는 색상을 기초로 문자열 캡차의 인식 성공률을 높일 수 있는 방법을 제안한다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제21권4호
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• pp.329-336
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• 2021

Background: In the field of dentistry, topical anesthetics play an important role in reducing pain during needle pricks. The anesthetic property of betel leaves remain unexplored, even though they have been widely used for the treatment of various ailments. The purpose of this study was to compare and evaluate pain perception following topical application of lignocaine gel, clove gel, ice, and newly developed betel leaf extract gel during intraoral injection in children. Methods: Sixty children aged 6-10 years who met the inclusion criteria were divided into four groups. Topical anesthetic agents, 2% lignocaine (Lox-2% Jelly, Neon, Mumbai, India), 4.7% clove gel (Pain Out Dental Gel, Colgate Palmolive India Ltd, Solan, India), 10% betel leaf extract gel, and ice were applied to each group for one min, followed by administration of infiltration anesthesia. Pain perception was analyzed during needle insertion. The Wong Bakers FACES pain rating scale (WBFPRS) was used for subjective assessment and the Sound, Eye, Motor (SEM) scale for objective assessment. Recorded values were tabulated and subjected to appropriate statistical analysis using SPSS software with a P value set at 0.05. Results: The clove oil and betel leaf groups demonstrated the highest WBFPRS scores, followed by the ice and lignocaine groups. The clove, betel leaf extract, and ice groups showed equal and highest SEM scores, followed by the lignocaine group. The mean WBFPRS and mean SEM scores were statistically significant. Conclusion: Betel leaf extract gel is effective in reducing pain and can act as an alternative topical anesthetic agent.

• 한국컴퓨터정보학회논문지
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• 제27권8호
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• pp.135-142
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• 2022

본 논문에서는 위급한 상황(예 : COVID-19)에서 바이러스 검사를 빠르게 진행하기 위한 그래프 기반 사용자 경로 제어와 이것을 도시 맵에서 시뮬레이션을 할 수 있는 프레임워크를 제안한다. 가상환경에서 많이 활용되는 길찾기(Pathfinding) 알고리즘인 A^*나 네비게이션 메쉬 자료구조는 정해진 정적 이동 경로만을 안내하기 때문에 가상환경에서 에이전트를 제어하는 CS(Computer science)문제에 적용할 할 경우 효율적이다. 하지만, 실제 COVID-19 환경에 적용하여 문제를 풀기에는 충분하지 않다. 특히, 빠른 바이러스 검사를 받기 위해서는 짧은 거리만을 이용하는 게 아닌, 실제 도로 교통상황, 병원의 크기, 환자 이동 수, 환자 처리 시간 등 고려해야 할 상황들이 많다. 본 논문에서는 위에서 언급한 다양한 속성들과 이를 이용한 최적화 함수를 모델링하여, 실제 도시 맵에서 바이러스 검사를 빠르고 효율적으로 처리할 수 있고, 다양한 상황을 디지털 트윈 방식으로 시뮬레이션을 할 수 있는 프레임워크를 제안한다.

• Restorative Dentistry and Endodontics
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• 제47권1호
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• pp.8.1-8.9
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• 2022

Objectives: The aim of this study was to compare the color change of the Giomer resin composite (Beautifil-Bulk) by using photographs obtained with a smartphone (iPhone 6S) associated with Adobe Photoshop software (digital method), with the spectrophotometric method (Vita Easyshade) after immersion in different pigment solutions. Materials and Methods: Twenty resin composite samples with a diameter of 15.0 mm and thickness of 1.0 mm were confectioned in A2 color (n = 5). Photographs and initial color readings were performed with a smartphone and spectrophotometer, respectively. Then, samples were randomly divided and subjected to cycles of immersion in distilled water (control), açai, Coke, and tomato sauce, 3 times a day, 20 minutes for 7 days. Later, new photographs and color readings were taken. Results: The analysis (2-way analysis of variance, Holm-Sidak, p < 0.05) demonstrated no statistical difference (p < 0.005) between the methods in all groups. Similar color changes were observed for all pigment solutions when using the spectrophotometric method. For the digital method, all color changes were clinically unacceptable, with distilled water and tomato sauce similar to each other and with statistical differences (p < 0.005) for Coke and açai. Conclusions: Only the tomato sauce produced a color change above the acceptability threshold using both methods of color assessment. The spectrophotometric and digital methods produce different patterns of color change. According to our results, the spectrophotometric method is more recommended in color change assessment.

• 국제학술발표논문집
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• The 8th International Conference on Construction Engineering and Project Management
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• pp.157-166
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• 2020

Automated rebar fabrication, which requires effective information exchange between model designers and fabricators, has brought the integration and interoperability of data from different sources to the notice of both academics and industry practitioners. Industry Foundation Classes (IFC) was one of the most commonly used data formats to represent the semantic information of prefabricated components in buildings, whereas the data format utilized by rebar fabrication machine is BundesVereinigung der Bausoftware (BVBS), which is a numerical data structure exchanging reinforcement information through ASCII encoded files. Seamless transformation between IFC and BVBS empowers the automated rebar fabrication and improve the construction productivity. In order to improve data interoperability between IFC and BVBS, this study presents an IFC extension based on the attributes required by automated rebar fabrication machines with the help of Information Delivery Manual (IDM) and Model View Definition (MVD). IDM is applied to describe and display the information needed for the design, construction and operation of projects, whereas MVD is a subset of IFC schema used to describe the automated rebar fabrication workflow. Firstly, with a rich pool of vocabularies practitioners, OmniClass is used in information exchange between IFC and BVBS, providing a hierarchy classification structure for reinforcing elements. Then, using International Framework for Dictionaries (IFD), the usage of each attribute is defined in a more consistent manner to assist the data mapping process. Besides, in order to address missing information within automated fabrication process, a schematic data mapping diagram has been made to deliver IFC information from BIM models to BVBS format for better data interoperability among different software agents. A case study based on the data mapping will be presented to demonstrate the proposed IFC extension and how it could assist/facilitate the information management.