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http://dx.doi.org/10.15429/jkomor.2018.18.2.57

Effects of Leejung-tang, Rikkunshito, and Bojungikgi-tang on Transient Receptor Potential Vanilloid 4 Channels  

Kim, Byung Joo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.18, no.2, 2018 , pp. 57-63 More about this Journal
Abstract
Objectives: Metabolic syndrome is defined by a cluster of major cardiovascular risk factors: obesity, insulin resistance, dyslipidemia, and arterial hypertension. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential vanilloid 4 (TRPV4) channels have been associated with the development of dyslipidemia and hypertension. The purpose of this study was to investigate the effects of Leejung-tang (Lizhong-tang), Rikkunshito, and Bojungikgi-tang (Buzhongyiqi-tang) on TRPV4 channel. Methods: Human embryonic kidney 293 cells stably transfected with the TRPV4 expression vectors were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2/10%$ $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software (Molecular Devices, San Jose, CA, USA), and signals were digitalized at 5 kHz using Digidata 1422A. Results: Leejung-tang and Rikkunshito (10, 30 and 50 mg/mL) had no effects on the TRPV4 whole-cell currents at dose dependent manner. However, Bojungikgi-tang (10, 30, and 50 mg/mL) inhibited the TRPV4 whole-cell currents in a dose dependent manner and the half maximal inhibitory concentration (IC50) of Bojungikgi-tang was 18.2 mg/mL. Conclusions: These results suggest that Bojungikgi-tang plays an important roles to inhibit the TRPV4 channel, suggesting that Bojungikgi-tang is considered one of the candidate agents for the treatment of metabolic syndrome such as dyslipidemia and hypertension.
Keywords
Metabolic syndrome; Leejung-tang; Rikkunshito; Bojungikgi-tang; Ion channel;
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1 Despres JP, Lemieux I. Abdominal obesity and metabolic syndrome. Nature. 2006 ; 444(7121) : 881-7.   DOI
2 Lee HW. Diagnosis and treatment of metabolic syndrome. Korean J Med. 2006 ; 71(4) : 463-7.
3 Jung SJ. The role of transient receptor potential channel in pain. Hanyang Med Rev. 2011 ; 31(2) : 116-22.   DOI
4 Clapham DE. TRP channels as cellular sensors. Nature. 2003 ; 426(6966) : 517-24.   DOI
5 Kim YM, Park YC, Jo JH, Kang WC, Son MW, Hong KE. Effect of herb medicine treatment for functional dyspepsia: a randomized placebo-controlled and compared standard t reatment trial. J Korean Med. 2010 ; 31(1) : 1-13.
6 Park YS, Yoon SH. Effect of Bojoongikki-tang, Sipjeondaebotang, Youngkaechulgam-tang on gastric contractility estimated by bowel sound in rats. Korean J Intern Med. 2010 ; 31(2) : 212-23.
7 Hiriart M, Velasco M, Larque C, Diaz-Garcia CM. Metabolic syndrome and ionic channels in pancreatic beta cells. Vitam Horm. 2014 ; 95 : 87-114.
8 Gao F, Wang DH. Impairment in function and expression of transient receptor potential vanilloid type 4 in Dahl salt-sensitive rats: significance and mechanism. Hypertension. 2010 ; 55(4) : 1018-25.   DOI
9 Earley S, Pauyo T, Drapp R, Tavares MJ, Liedtke W, Brayden JE. TRPV4-dependent dilation of peripheral resistance arteries influences arterial pressure. Am J Physiol Heart Circ Physiol. 2009 ; 297(3) : H1096-102.   DOI
10 Lakk M, Vazquez-Chona F, Yarishkin O, Krizaj D. Dyslipidemia modulates Muller glial sensing and transduction of ambient information. Neural Regen Res. 2018 ; 13(2) : 207-10.   DOI
11 Mamenko MV, Boukelmoune N, Tomilin VN, Zaika OL, Jensen VB, O'Neil RG, et al. The renal TRPV4 channel is essential for adaptation to increased dietary potassium. Kidney Int. 2017 ; 91(6) : 1398-409.   DOI
12 Sanchez JC, Rivera RA, Munoz LV. TRPV4 Channels in human white adipocytes: electrophysiological characterization and regulation by insulin. J Cell Physiol. 2016 ; 231(4) : 954-63.   DOI
13 O'Conor CJ, Leddy HA, Benefield HC, Liedtke WB, Guilak F. TRPV4-mediated mechanotransduction regulates the metabolic response of chondrocytes to dynamic l oading. Proc Natl Acad Sci USA. 2014 ; 111(4) : 1316-21.   DOI
14 Kim D, Kim JN, Nam JH, Lee JR, Kim SC, Kim BJ. Modulation of pacemaker potentials in murine small intestinal interstitial cells of Cajal by Gamisoyo-San, a traditional Chinese herbal medicine. Digestion. 2018 ; 98(1) : 56-68.   DOI
15 Pedersen SF, Owsianik G, Nilius B. TRP channels: an overview. Cell Calcium. 2005 ; 38(3-4) : 233-52.   DOI
16 Nam HJ, Chae BY. The experimental study in the anti-allergic effects of Leejungtang and Leejungtanggamibang. J Kor Ori Med Ophthalmol Otolaryngol Dematol. 2000 ; 13(1) : 60-77.