• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.413-418
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• 2009

This study was performed to investigate the changes of the serological parameters of the rats fed a high fat and high cholesterol diets with or without quercetin supplement for five weeks. Thirty Sprague-Dawley male rats ($152.1{\pm}17.0\;g$ of body weight) were randomly divided into three groups with 10 rats in each group. Rats in the control(C) group were fed the high fat and high cholesterol diet containing 15% lard, 2% cholesterol and 0.5% sodium cholate (w/w). Rats in two treatment groups were fed the same diet supplemented with 0.25% quercetin (Q-0.25) or 0.5% quercetin (Q-0.5) on the weight to weight basis, respectively. The final body weight, gain of body weight, the amount of feed intake and the feed efficiency of rats in between control and treatment groups were not significantly different. Serum glucose, total protein, albumin, globulin and albumin/globulin (A/G) ratio of rats showed no significant differences between control and treatment groups. Serum total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and atherogenic index(AI) of rats in both Q-0.25 and Q-0.5 groups were significantly lower than in C group (p < 0.05). However serum high density lipoprotein-cholesterol (HDL-C) was significantly higher in both Q-0.25 and Q-0.5 groups than in C group (p < 0.05). The levels of triglyceride in sera of rats showed no significant differences between control and treatment groups. The values of AST and ALT in sera of rats showed no significant differences between control and treatment groups. Therefore the supplementation of quercetin to high fat and high cholesterol diet in rats was effective in reducing the levels of serum lipids to cause cardiovascular diseases and in elevating the level of HDL-C to protect cardiovascular diseases.

• Food Science and Biotechnology
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• v.16 no.2
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• pp.249-254
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• 2007

The effects of chronic chitosan salt supplementation on the systolic (SBP) and diastolic blood pressure (DBP), and physiological parameters were investigated in healthy male and female adult. Chitosan salt was conducted by measuring various health-related factors such as body composition, plasma $Na^+$, $Cl^-$, lipid, and lipoproteins profiles, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activity. Chitosan salt supplementation no significant differences before and after supplement in body composition variables and in SBP and DBP in either male or female. Plasma sodium and chlorine concentration no significant changes during chitosan salt supplementation, and no significant difference between two genders. Plasma GOT and GPT activity no different before and after supplement in either male or female. GOT activity significantly higher for male before supplement (p<0.05), and 2 weeks after supplement (p<0.01). The lipid and lipoproteins profiles of plasma no significant changes during chitosan salt supplementation in either male or female subjects. In summary, the chronic intake of chitosan salt did not affect the SBP or DBP, and posed no health risks.

• Journal of Pharmacopuncture
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• v.17 no.3
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• pp.7-15
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• 2014

Objectives: The present study investigated the toxic properties of petroleum ether extract of Wattakaka (W.) volubilis in Wistar female rats. Methods: An in vitro brine shrimp lethality bioassay was studied in A. Salina nauplii, and the lethality concentrations were assessed for petroleum ether extract of W. volubilis. A water soluble portion of the test extract was used in different concentrations from $100-1000{\mu}g/mL$ of 1 mg/mL stock solution. A 24-hours incubation with a 1-mL aliquot in 50 mL of aerated sea water was considered to calculate the percentage rate of dead nauplii with test extract administration against a potassium-dichromate positive control. The acute and the sub-acute toxicities of petroleum ether extract of W. volubilis were evaluated orally by using gavage in female Wistar rats. Food and water intake, body weight, general behavioral changes and mortality of animals were noted. Toxicity or death was evaluated following the administration of petroleum ether extract for 28 consecutive days in the female rats. Serum biochemical parameters, such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, total cholesterol, triglyceride, total protein, glucose, urea, creatinine, sodium, potassium and ${\alpha}$-amylase levels, were measured in the toxicity evaluations. Pathological changes in isolated organs, such as the liver, kidneys, and pancreas, were also examined using hematoxylin and eosin dye fixation after the end of the test extract's administration. Results: The results of the brine-shrimp assay indicate that the evaluated concentrations of petroleum ether extract of W. volubilis were found to be non-toxic. In the acute and the sub-acute toxicity evaluations, no significant differences were observed between the control animals and the animals treated with extract of W. volubilis. No abnormal histological changes were observed in any of the animal groups treated with petroleum ether extract of W. volubilis. Conclusion: These results suggest that petroleum ether extract of W. volubilis has a non-toxic effect in Wistar female rats.

• Journal of Veterinary Clinics
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• v.22 no.2
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• pp.108-113
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• 2005

This study was performed to investigate the growth rate, hematological and serological changes of the rats when they were fed with the high fat diets supplemented with or without the tannic acid for five weeks. Thirty-two Sprague-Dawley male rats(235.7\pm10.7g\;of\;body\;weight)$ were randomly divided into four groups, control group and three treatment groups(T1, T2 and T3). Rats in control group were fed with the high fat diet containing $15\%\;lard,\;1\%$ cholesterol and $0.5\%$ sodium cholate(wt/wt) which was modified from the formula of American Institute of Nutrition (AIN)-76 diet and rats in treatment groups were fed with above diet supplemented with $0.25\%(T1),\;0.5\%(T2)$ or $0.75\%(T3)$ of tannic acid(wt/wt), respectively. The supplementation of tannic acid(TA) did not affect the final body weight, gain of body weight and feed intake of rats in both control and treatment groups. The numbers of red blood cells, hemoglobin concentrations and hematocrit values in blood of rats showed no significant differences between control group and treatment groups. The glucose concentration and albumin/globulin(A/G) ratio of rats in treatment groups were slightly lower than that of control group without significance. The values of total protein, albumin and globulin showed no significant differences between control group and treatment groups. The values of total cholesterol, low density lipoprotein­cholesterol and atherogenic index in sera of rats in treatment groups were much lower than that of control group without significance. The values of triglycerides in sera of rats in T3 group were significantly lower than that of control group (p<0.05). The values of AST and ALT in sera of rats in T3 group were significantly lower than that of control group (p<0.05). Thus supplementation of tannic acid to high fat diet could be effective to reduce the serum lipid levels such as total cholesterol, high density lipoprotein-cholesterol and triglycerides which were regarded as to cause the cardiovascular diseases.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.3
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• pp.129-138
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• 1978

The contents of inorganic conservative constituents in the downstream water were determined in spring tides of every month from May 1977 to April 1978 at eight stations of Nagdong River. Samples were taken at the intervals of one or two hours from 7 a. m. to 7 p. m. at each station. Annual ranges and means of the chemical constituents over all the stations except station one, Kupo, ate as follows: pH 6.4-9.3, 7.7; electrical conductivity $0.085-0.345\times10^3\;\mu\mho/cm,\;0.196\times10^3\;\mu\mho/cm;$ chloride 5.8-50.0ppm, 17.7ppm: fluoride ND-0.19 ppm, 0.06 ppm: sulfate 5.5-41.1 ppm, 20.7 ppm; calcium 6-26 ppm, 17 ppm; magnesium 2.0-12.8 ppm, 5.1 ppm; sodium 7-26 ppm, 13 ppm; potassium 1.4-3.8 pprn, 2.3 ppm respectively. The seasonal variations of contents of the chemical constituents were not large and showed nearly definite values at all the stations except station one, Kupo. At station one, seasonal variations were large and the contents were excessively high due to inflow of seawater compared with other stations. The values over 50 ppm in chloride were not determined during the determination period at Mul Geum where the intake station for Busan city water is located. Most constituents except pH and fluoride were over the criteria for drinking water at Kupo, while at other stations only pH value was exceeded the upper limit of the criterion especially in summer period. The pH values tended to increase in the afternoon when water temperature was high. The chloride concentration was shown the highest value at station one, Kupo, with about 2 hours delay after high water of Busan harbour and 3-3.5 hours at Mul Geum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.1
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• pp.233-239
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• 1999

This study was performed to evaluate the effects of the supplementation of lactic acid bacterias (LAB; S. thermophilus, L. acidophilus) aluminum accumulation and function kidney in rats treated with 250 g/g aluminum sulfate for 4 weeks. Fifty male Sprague Dawley strains were divided into five groups according to the types of supplement. the control, S. thermophilus culture group(A), autoclaved S. thermophilus culture(B), L. acidophilus culture group(C) and autoclaved L. acidophilus(D). The weight gain was increased by supplementation of S. thermophilus culture(A), autoclaved S. thermophilus culture(B), L. acidophilus culture(C), autoclaved L. acidophilus culture(D), especially by supplementation of S. thermophilus cultured(A) as compared to control group. The amount of water intake was increased in control group as compared to the LAB supplemented group. The content of aluminum were decreased 19.57%, 31.25%, 37.10%, and 32.40% in kidney after supplementation of non autoclaved culture group (A,C), and autoclaved group(B,D) respectively, as compared to control group. Water balance, urine volume, and excretion of sodium and potassium decreased in LAB supplemented group and control group. Excretion of creatinine increased in control group and tendency to increased in LAB supplemented group. In conclusion, the effect of suppression of aluminum accumulation was more effective in sup plementation of S. thermophilus culture(A), and L. acidophilus culture(C) than autoclaved S. thermo philus culture(B), and L. acidophilus culture(D).

• Journal of the East Asian Society of Dietary Life
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• v.27 no.5
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• pp.500-511
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• 2017

This study was conducted to investigate the anthropometric data, bone density, serum profiles, nutrient intakes and diet quality of premenopausal and postmenopausal women. For the study, we obtained data for analysis from the combined 2008~2011 Korean National Health and Nutrition Examination Survey (KNHANES). The height and weight were 157.8 cm and 58.7 kg in premenopausal women and 155.5 cm and 58.3 kg in postmenopausal women, respectively. The obesity rate was 27.49% in premenopausal women and 34.98% in postmenopausal women (p<0.001). Total cholesterol, LDL-cholesterol, triglyceride, GOT, GPT and alkaline phosphatase in postmenopausal women were significantly higher than those in premenopausal women (p<0.001). The prevalence of osteoporosis was 0.0~0.89% in premenopausal women and 0.48~13.22% in postmenopausal women (p<0.001). In postmenopausal women, rates of hypertension, stroke, myocardial infarction, depression, and diabetes were significantly higher than those in premenopausal women. Water, fat. ash, sodium, retinol, thiamin, riboflavin and niacin intakes in premenopausal women were significantly higher than those in postmenopausal women. Water, fiber, Ca, and, K intakes were below KDRIs (Dietary Reference Intakes for Koreans) in both groups. The mean adequacy ratio (MAR) of premenopausal women was higher than that of postmenopausal women (p<0.001). The index of nutritional quality (INQ) in premenopausal women was also higher than that of postmenopausal women except iron and vitamin C. Therefore dietary guidelines and an education program should be developed for desirable improvement of health, bone density, nutrient status and dietary quality of postmenopausal women.

• Journal of the East Asian Society of Dietary Life
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• v.25 no.2
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• pp.372-385
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• 2015

The purpose of this study was to develop and evaluate a nutritional education program for preschool children in association with Center for Children's Foodservice Management, childcare facilities and home in order to provide nutritional knowledge and change food attitudes in children. The program was repeated three times using the same educational theme and consisted three steps. Step 1 involved visiting education with teaching tools by a dietician. Step 2 involved home education with a worksheet and participating in events with parents. Step 3 involved repeated education with a textbook by a preschool teacher education reports sent to the center. The subjects of this study were 3 to 5-year-old children at 89 childcare facilities located in Busanjingu. Interest in meals at childcare facilities, dietary guidance for parents of children, and dietary attitudes of children were evaluated before and after education. Number of parents interested in meals managed by childcare facilities (kitchen visiting, meals observation, progress of children's education) increased after education. Mean scores for meal awareness in parents in the form of three questions (proper food distribution, hand washing before mealtime, nutritional and hygienic satisfaction with meals) significantly (p<0.05) increased after education. Mean dietary guidance for parents' scores for all questions except table manner significantly (p<0.01) increased after education. Mean dietary attitudes for children in the form of six questions (try to eat various vegetable, eat meals without leaving, wash hands before mealtime, eat cleanly, reduce sodium intake and keep table manner) also significantly (p<0.001) increased after education. As a results, the nutritional education program applied in this study, positive influenced children's nutritional knowledge and dietary attitudes, and parents' dietary guidance of children.

• Korean Journal of Community Nutrition
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• v.21 no.5
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• pp.440-450
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• 2016

Objectives: We previously developed a dish-based semi-quantitative food frequency questionnaire (FFQ) for Korean adolescents and reported that it had reasonable reliability and validity. The objective of the current study was to construct a web-based dietary evaluation system applying the FFQ for Korean adolescents and examine its applicability in the context of reliability and validity. Methods: A web-based food frequency questionnaire system was designed using a comprehensive approach, incorporating not only dietary data survey but also up-to-date nutrition information and individualized eating behavior guidelines. A convenience sample of 50 boys and girls aged 12~18 years agreed to participate in the study and completed the FFQ twice and 3 days of dietary recall on the developed website during a two-month period. The FFQ's reliability and validity was examined using correlation and cross classification analysis. We also measured participants' subjective levels of the web site's usability, visual effect, understanding, and familiarity. Results: Spearman correlation coefficients for reliability ranged from 0.74 (for vitamin A) to 0.94 (for energy). From cross-classification analyses, the proportion of subjects in the same intake quartile was highest for energy (82.0%) and lowest for vitamin A (56.0%). With regard to validity analysis, Spearman correlation coefficients ranged from 0.34 (for fiber) to 0.79 (for energy). The proportions of subjects in the opposite categories between the first FFQ and 3-day diet recall data were generally low from 0.00% (for fat) to 36.2% (for sodium). Average subjective levels of the website's usability, visual effect, understanding, and familiarity were all found to be over 4 points out of 5 points. Conclusions: The web-based dietary evaluation system developed can serve as a valid and attractive tool for administering FFQ to Korean adolescents.

• Nutrition Research and Practice
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• v.12 no.2
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• pp.135-142
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• 2018

BACKGROUND/OBJECTIVES: This cross-sectional study assessed household food security status and determined its association with diet quality and weight status among indigenous women from the Mah Meri tribe in Peninsular Malaysia. SUBJECTS/METHODS: The Radimer/Cornell Hunger and Food Insecurity Instrument and the Malaysian Healthy Eating Index (HEI) were used to assess household food security status and diet quality, respectively. Information on socio-demographic characteristics and 24-hour dietary recall data were collected through face-to-face interview, and anthropometric measurements including weight, height, and body mass index (BMI) were obtained from 222 women. RESULTS: Majority of households (82.9%) experienced different levels of food insecurity: 29.3% household food insecurity, 23.4% individual food insecurity, and 30.2% fell into the child hunger group. The food-secure group had significantly fewer children and smaller household sizes than the food-insecure groups (P < 0.05). The mean household income, income per capita, and food expenditure significantly decreased as food insecurity worsened (P < 0.001). The food-secure group had significantly higher Malaysian HEI scores for grains and cereals (P < 0.01), as well as for meat, poultry, and eggs (P < 0.001), than the food-insecure groups. The child-hunger group had significantly higher fat (P < 0.05) and sodium (P < 0.001) scores than the food-secure and household food-insecure groups. Compared to the individual food-insecure and child-hunger groups, multivariate analysis of covariance showed that the food-secure group was significantly associated with a higher Malaysian HEI score while the household food-insecure group was significantly associated with a higher BMI after controlling for age (P < 0.025). CONCLUSIONS: The majority of indigenous households faced food insecurity. Food insecurity at the individual and child levels was associated with lower quality of diet, while food insecurity at the household level was associated with higher body weight. Therefore, a substantial effort by all stakeholders is warranted to improve food insecurity among poorer households. The results suggest a pressing need for nutritional interventions to improve dietary intake among low income households.