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Effect of Quercetin Supplement on Major Biochemical Parameters in Sera of Rats Fed High Fat and High Cholesterol Diet  

Hwang, Eui-Kyung (Department of Animal Science, College of Life Science and Natural Resources, Sangji University)
Publication Information
Journal of Veterinary Clinics / v.26, no.5, 2009 , pp. 413-418 More about this Journal
Abstract
This study was performed to investigate the changes of the serological parameters of the rats fed a high fat and high cholesterol diets with or without quercetin supplement for five weeks. Thirty Sprague-Dawley male rats ($152.1{\pm}17.0\;g$ of body weight) were randomly divided into three groups with 10 rats in each group. Rats in the control(C) group were fed the high fat and high cholesterol diet containing 15% lard, 2% cholesterol and 0.5% sodium cholate (w/w). Rats in two treatment groups were fed the same diet supplemented with 0.25% quercetin (Q-0.25) or 0.5% quercetin (Q-0.5) on the weight to weight basis, respectively. The final body weight, gain of body weight, the amount of feed intake and the feed efficiency of rats in between control and treatment groups were not significantly different. Serum glucose, total protein, albumin, globulin and albumin/globulin (A/G) ratio of rats showed no significant differences between control and treatment groups. Serum total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and atherogenic index(AI) of rats in both Q-0.25 and Q-0.5 groups were significantly lower than in C group (p < 0.05). However serum high density lipoprotein-cholesterol (HDL-C) was significantly higher in both Q-0.25 and Q-0.5 groups than in C group (p < 0.05). The levels of triglyceride in sera of rats showed no significant differences between control and treatment groups. The values of AST and ALT in sera of rats showed no significant differences between control and treatment groups. Therefore the supplementation of quercetin to high fat and high cholesterol diet in rats was effective in reducing the levels of serum lipids to cause cardiovascular diseases and in elevating the level of HDL-C to protect cardiovascular diseases.
Keywords
quercetin; cholesterol; HDL-C; LDL-C; rat;
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1 Basile A, Sorbo S, Sorbo S, Giodano S, Ricciardi L, Ferrara S, Montesano D, Cobianchi RC, Vuotto ML, Ferrara L. Antibacterial and allelopathic activity of extract from Castanea sativa leaves. Fitoterapia 2000; 71: S110-S116   DOI   ScienceOn
2 Bok SH, Park SY, Park YB, Lee MK, Jeon SM, Jeong TS, Choi MS. Quercetin dihydrate and gallate supplements lower plasma and hepatic lipids and change activities of hepatic antioxidant enzymes in high cholesterol-fed rats. Int J Vitam Nutr Res 2002; 72: 161-169   DOI   ScienceOn
3 Choi EJ, Chee KM, Lee BH. Anti- and prooxidant effects of quercetin administration in rats. Eur J Pharmacol 2003; 482: 281-285   DOI   PUBMED   ScienceOn
4 Cook NC, Samman S. Flavonoids - Chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem 1996; 7: 66-76   DOI   ScienceOn
5 Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. High density lipoprotein as a protective factor against coronary heart disease. Am J Med 1977; 62: 707-714   DOI   ScienceOn
6 Gl$\ddot{a}$$\beta$er G, Graefe EU, Struck F, Veit M, Gebhardt R. Comparison of antioxidative capacities and inhibitory effects on cholesterol biosynthesis of quercetin and potential metabolites. hytomedicine 2002; 9: 33-40   DOI   ScienceOn
7 Igarashi K, Ohmuma M. Effects of isohamnetin, rhamnetin, and quercetin on the concentrations of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities of rats. Biosci Biotech Biochem 1995; 59: 595-601   DOI   ScienceOn
8 Kannel WB, Castelli WP, Gordon T, McNamara PM. Serum cholesterol, lipoproteins, and the risk of coronary heart disease. Ann Intern Med 1971; 74: 1-12   PUBMED   ScienceOn
9 Manach C, Morand C, Demigne C, Texier O, Régérat F, Remesy C. Bioavailability of rutin and quercetin in rats. FEBS Letters 1997; 409: 12-16.   DOI   ScienceOn
10 Mott GE, Jackson EM, McMahan CA, McGill HC Jr. Dietary cholesterol and type of fat differentially affect cholesterol metabolism and atherosclerosis in baboons. J Nutr 1992; 122: 1397-1406   PUBMED
11 Peterson J, Dwyer J. Flavonoids: Dietary occurrence and biochemical activity. Nutr Res 1998; 18: 1995-2018   DOI   ScienceOn
12 Steinberg D. The rediscovery of high density lipoprotein: a negative risk factor in atherosclerosis. Eur J Clin Invest 1978; 8: 107-109   DOI   PUBMED   ScienceOn
13 Yugarani T, Tan BKH, Teh M, Das NP. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 1992; 27: 181-186   DOI   ScienceOn
14 Hirono I, Ueno I, Hosaka S, Takanashi H, Matsushima T, Sugimura T, Natori S. Carcinogenicity examination of quercetin and rutin in ACI rats. Cancer Letters 1981; 13: 15-21   DOI   ScienceOn
15 Amresh G, Zeashan H, Gupta RJ, Kant R, Rao CV, Singh PN. Gastroprotective effects of ethanolic extract from Cissampelos pareira in experimental animals. J Nat Med 2007; 61: 323-328   DOI   ScienceOn
16 Dunnett CW. A multiple comparison procedure for comparing several treatments with a control. Am Stat Assoc J 1955; 50: 1096-1121   DOI   ScienceOn
17 Manach C, Morand C, Texier O, Favier M-L, Agullo G, Demign$\acute{e}$ C, R$\acute{e}$g$\acute{e}$rat F, R$\acute{e}$m$\acute{e}$sy C. Quercetin metabolites in plasma of rats fed diets containing rutin or quercetin. J Nutr 1995; 125: 1911-1922   PUBMED   ScienceOn
18 김형진, 송시환, 하창수, 한상섭. 사육밀도가 Sprague-Dawley 랫드의 성장 및 각종 생리치에 미치는 영향. 한국실험동물학회지 1993; 9: 71-82
19 송창우, 황화선, 한상섭. Ktc: SD 랫드의 주령에 따른 기초 연구 I. 체중변화, 혈액·혈액생화학적 변화 및 뇨분석. 한국실험동물학회지 1990; 6:33-43
20 Janbaz KH, Saeed SA, Gilani AH. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine 2004; 11: 424-430   DOI   ScienceOn
21 Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Chem 1992; 40: 2379-2383   DOI
22 Hou L, Zhou B, Yang L, Liu ZL. Inhibition of human low density lipoprotein oxidation by flavonols and their glycosides. Chem Phys Lipids 2004; 129: 209-219   DOI   ScienceOn
23 Ueno I, Nakano N, Hirono I. Metabolic fate of [$^{14}C$] quercetin in the ACI rat. Japan J Exp Med 1983; 53: 41-50   ScienceOn
24 Friewald WT, Levy RI, Fredrickson DS. Estimation of concentration of low density lipoprotein cholesterol without use of the preparation of ultracentrifuge. Clin Chem 1972; 28: 499-502
25 Lee E, Choi EJ, Cheong H, Kim YR, Ryu SY, Kim KM. Antiallergic actions of the leaves of Castanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation. Arch Pharm Res 1999; 22: 320-323   DOI   ScienceOn
26 Erlund I. Review of the flavonoids quercetin, hesperetin, and naringenin. Dietary sources, bioactivities, bioavailability, and epidemiology. Nutr Res 2004; 24: 851-874   DOI   ScienceOn
27 Exon JH, Magnuson BA, South EH, Hendrix K. Dietary quercetin, immune functions and colonic carcinogenesis in rats. Immunopharmacol Immunotoxicol 1998; 20: 173-190   DOI   ScienceOn
28 Haglund O, Luostarinen R, Wallin R, Wibell L, Saldeen T. The effects of fish oil on triglyceride, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur J Nutr 1991; 121: 165-169
29 Holmes DL. Clinical laboratory animal medicine. Ames: Iowa State University Press 1984: 110-111
30 Aherne SA, O'Brien NM. Protection by the flavonoids myricetin, quercetin, and rutin against hydrogen peroxideinduced DNA damage in Caco-2 and Hep G2 cells. Nutr Cancer 1999; 34: 160-166   DOI   ScienceOn
31 Mitruka BM, Rawnsley HM. Clinical biochemical and hematological reference values in normal experimental animals and normal humans. 2nd ed New York: Masson Publishing USA, Inc 1981: 57-166
32 Packard CJ, McKinney L, Carr K, Shepherd J. Cholesterol feeding increases low density lipoprotein synthesis. J Clin Invest 1983; 72: 45-51   DOI   PUBMED
33 Crespy V, Morand C, Manach C, Besson C, Demigne C, Remesy C. Part of quercetin absorbed in the small intestine is conjugated and further secreted in the intestinal lumen. Am J Physiol Gastrointest Liver Physiol 1999; 277: 120-126
34 Lee JH, Kang YH, Kang JS. Free radical scavenging of flavonoids and their effects on erythrocyte Na leak, platelet aggregation and TBARS production. Nutr Sci 2002; 5: 197-202   과학기술학회마을
35 Havsteen BH. The biochemistry and medical significance of the flavonoids. Phamacol Therap 2002; 96: 67-202   DOI   ScienceOn
36 Manach C, Scalbert A, Morand C, R$\acute{e}m$\acute{e}$sy C, Jim$\acute{e}$nez L. Polyphenols: food and bioavailability. Am J Clin Nutr 2004; 79: 727-747   PUBMED   ScienceOn
37 Rao CV, Vijayakumar M. Effect of quercetin, flavonoids and α-tocopherol, an antioxidant vitamin on experimental reflux oesophagitis in rats. Eur J Pharmacol2008; 589: 233-238   DOI   PUBMED   ScienceOn
38 Rathi AB, Nath N, Chari SN. Action of vitamin P like compounds on lysosomal status in hypercholesterolemic rats. Acta Vitaminol Enzymol 1883; 5: 255-261   ScienceOn
39 이삼열, 정윤섭, 권오헌, 송경순. 임상병리검사법 7판 서울: 연세대학교 출판부 2000: 224-315
40 Mattson FH, Erickson BA, Kligman AM. Effect of dietary cholesterol on serum cholesterol in man. Am J Clin Nutr 1972; 25: 589-594   PUBMED   ScienceOn
41 The American Institute of Nutrition : Report of American Institue of Nutrition Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 1977; 107:1340-1348   PUBMED
42 Crespy V, Morand C, Besson C, Manach C, Demigne C, Remesy C. Quercetin, but not its glycosides, is absorbed from the rat stomach. J Agric Food Chem 2002; 50: 618-621   DOI   ScienceOn
43 Basarkar PW, Nath N. Hypocholesterolemic and hypolipidemic activity of quercetin-a vitamin P-like compound in rats. Indian J Med Res 1983; 77: 122-126   PUBMED
44 Seo HJ, Kim SJ, Do GM, Choi MS. Quercetin supplement is beneficial for altering lipid metabolism and antioxidant enzyme activities in the middle of ethanol feeding in rats. Nutr Sci. 2006; 9: 259-266   과학기술학회마을
45 Formica JV, Regelson W. Review of the biology of quercetin and related bioflavonoids. Fd Chem Toxic 1995; 33: 1061-1080   DOI   ScienceOn
46 Robak J, Gryglewski R. Flavonoids are scavengers of superoxide anions. Biochem Phamacol 1988; 37: 837-841   DOI   ScienceOn