• 미생물학회지
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• 제21권3호
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• pp.115-126
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• 1983

The results that the effect of 6 detergents on the structural changes and biochemical composition of bacterial membrane of Escherichia coli and Bacillus cereus are as follows ; 1. Population growth of the bacteria was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was decreased by sodium dodecyl sulfate and palmitoyl choline, in E.coli and was decreased by palmitoyl carnitine and palmitoyl choline at the low concentration, in B. cereus. 2. The electron micrograph showed that cell wall lysis or cell collapse were observed in the treatment of sodium dodecyl sulfate and palmitoyl choline, and also cell wall was condensed by triton X-100 and sodium deoxy cholate, in E.coli. And in B. cereus, endospore formation of the bacteria was stimulated by palmitoyl choline, and cell lysis or structural changes of the membrane were observed in the treatment of sodium dodecyl sulfate, sodium cholate, and triton X-100, respectively. 3. As to the effect of detergent on the biochemical composition of biomembrane, the content of carnitine, in E.coli, and B.cereus, the content of structural protein and phospholipid were decreased by treatment of sodium dodecyl sulfate and structural protein was denatured by palmitoyl choline. 4. The profile of membrane protein revealed that the bacterial membrane were composed of various proteins. By dint of this result, some of membrane proteins were solubilized or changed to small molecules by the treatment of sodium dodecyl sulfate and palmitoyl choline, in E.coli and membrane protein of the biomembrane by treatment of sodium dodecyl sulfate, sodium deoxy cholate, palmitoyl choline, and palmitoyl carnitine were confirmed to be different profile as compared with those of the control, in B. cereus. Therefore, it is suggested that sodium dfodecyl sulfate and palmitoyl choline soulbilized biomembranes or inhibited membrane transport and that palmitoyl carnitine and sodium deoxy cholate were used as an energy source or stimulating the membrane transport, in E.coli. And, it is suggested that all of detergents were inhibited biomembrane synthesis, expet saponin, in B.cereus.

• 한국식생활문화학회지
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• 제35권4호
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• pp.351-362
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• 2020

This study investigated the possibility of reducing sodium and sugar intake in the Bulgogi bowl and yogurt, respectively, by utilizing food and non-food factors. Sodium and sugar replacers were the target food factors, and eating environment was the target non-food factor. The sodium content of Bulgogi bowls was reduced by 40%, and a flavor enhancer was applied to compensate for the reduced saltiness. Low calorie sweeteners, such as sucralose and stevia, were used to lower the sugar content of yogurt by 30%. Consumers were asked to evaluate the samples in one of the three eating environments: private booth evaluation, BOOTH; watching video clips during evaluation, VIDEO; conversation with friends during evaluation, FRIEND. The results showed that applying sodium or sugar replacers were effective in lowering sodium or sugar intake, respectively, without compromising the acceptance level of samples. Significant influences of the eating environment were observed on liking and perception of samples, but the influence patterns were not consistent between Bulgogi bowl and yogurt.

• 한국식품영양과학회지
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• 제45권5호
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• pp.757-764
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• 2016

본 연구는 경북지역 남녀공학 중학교에서 제공된 점심급식의 소금 및 나트륨 함량에 대해 알아보기 위해 칠곡지역 4개 중학교를 대상으로 한 끼당 총 소금 및 나트륨 함량과 한끼 소금 함량 기여도를 조사하였으며 그 결과는 다음과 같다. 조사대상 학교의 한 끼당 전체 평균 소금 함량은 4.41g이며, 전체 평균 나트륨 함량은 1,736.02 mg이다. 4개 학교에서 제공한 총 40개의 식단 중 1개 식단을 제외한 39개(97.5%) 식단이 식약처에서 권고하는 중학교 '삼삼급식소' 나트륨 함량 기준(1,000 mg)을 초과하는 것으로 나타났다. 특히 조사기간 동안 제공된 점심급식 중 나트륨 함량이 가장 높은 식단은 2,827.56 mg으로 나타났다. 점심급식에서 제공된 메뉴를 총 16개 군으로 분류하여 음식군별로 제공량, 염도, 소금 및 나트륨 함량을 조사한 결과 전체 평균 염도는 1.06%이며, 전체 평균 소금 함량은 0.74 g이다. 조사대상 학교에서 점심급식으로 제공된 음식을 밥류, 일품밥 면류, 국 탕 찌개류, 주찬, 부찬, 김치류, 후식류 등의 7개 메뉴 유형별로 구분하여 한 끼 소금 함량 기여도를 조사한 결과 일품밥 면류(40.56%), 국 찌개류(23.23%), 김치류(20.30%), 주찬류(18.13%), 부찬류(11.36%) 순으로 높게 나타났다. 조사기간 동안 제공된 학교별 국 탕 찌개류의 염도는 전체 평균 0.75%이며, 38개 국 탕 찌개 중 12개(32.0%)가 식약처 국염도 권고기준 0.8%를 초과하는 것으로 나타났다. 일품밥 면류와 주찬류 중 나트륨 함량이 높은 메뉴를 살펴본 결과 일품밥 면류는 쌀잔치국수(1,056.49 mg), 자장볶음밥(976.48 mg), 오므라이스(821.33 mg), 굴소스야채볶음밥(791.00 mg), 비빔밥(744.46 mg) 등의 순으로 높게 나타났으며, 주찬류는 오징어미더덕찜(1,120.88 mg), 돈육버섯볶음(626.84 mg), 김치돈육볶음(563.28 mg), 매운어묵조림(498.01 mg), 떡볶이(497.45 mg) 등의 순으로 조사되었다. 이상의 연구 결과를 통해 중학교 급식 이용자의 중식을 통한 나트륨 섭취량 감소를 위해서는 한 끼 총나트륨 함량을 1,000 mg 이하 수준으로 제공하는 것을 목표로 하는 '삼삼급식소' 사업에 학교급식이 적극적으로 참여하여 학교급식에 나트륨 저감화가 정착되도록 힘써야 할 것으로 생각한다.

• 한국식품위생안전성학회지
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• 제4권1호
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• pp.37-44
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• 1989

멸치를 물로 씻은 후 식염 10%, 15%, 20% 및 식염과 염화칼리 1:1 홈합염 10%를 각기 첨가하여 1~12주간 숙성할 때 불휘발성 아민의 함량과 미생물의 변화와 시중에 유통중인 멸치젓의 불휘발성아민 함량을 조사하였다. 불휘발성아민은 식염농도가 높을수록 생성량이 적었고 숙성기간별 생성량은 histamine의 경우 숙성 초기부터 증가하여 6주째에 식염 10%, 멸치젓이 657.8ppm으로 최고치를 나타낸 후 감소하기 시작하여 숙성 12주째는 식염 20%, 멸치젓이 210.8ppm으로 가장 적었고 혼합염 10% 멸치젓이 310.8ppm으로 가장 많이 생성되었다. Cadaverine과 putrescine은 숙성 8주째까지 생성량이 증가한 후 점차적으로 줄어들었고 tyramine은 숙성 10주째까지 RPthrwmd가한 뒤 숙성 12주째 현저히 감소하였다. 시중 유통품과 식염 함량이 비슷한 20% 멸치젓의 12주째 실험결과를 비교하면 시중 유통품의 불휘발성 아민 함량이 전반적으로 높게 나타났다. 미생물의 변화는 숙성 초기에는 Achromobacter 속, Aeromonas 속 및 Pseudomonas속이, 숙성 중기에는 Pediococcus속 , Microc....us 속 및 Lactobacillus 속이 많았으며 숙성 후기에는 Sacchromyces 속이 증가하였고 전 숙성기간 동안 미생물은 109주가 분리되었다.

• 대한지역사회영양학회지
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• 제25권4호
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• pp.280-290
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• 2020

Objectives: This study investigated the menu and nutritional contents of convenience store lunchboxes, and evaluated the nutritional content by meal type, price, and store brand. Methods: In September 2019, 93 convenience store lunchboxes from the top five franchise stores were purchased. Relevant information on price, food weight, food ingredients, cooking methods, and nutrition labeling were subsequently collected. Nutritional content was evaluated based on the daily value (DV) and Index of Nutritional Quality (INQ), and energy contribution of carbohydrate, protein, fat, saturated fat and sugar was compared with the recommended range. Results: Most lunchboxes included the food groups; grains/starches, meats/fish/eggs/legumes, and vegetables. However, none provided fruits, and only a few lunchboxes provided milk/milk products. Stir-frying, deep-frying, and pan-frying were the most frequent methods of cooking. The average energy content of the lunchboxes was 736.6 kcal, whereas the average contents of protein, fat and saturated fat were higher than 40% of the DV, and sodium content was 66.8% of the DV. The contents of most nutrients in traditional type lunchboxes were higher, as compared to nutrients in onedish type lunchboxes. Considering pricing of lunchboxes, protein and sodium contents were higher in the higher-priced lunchboxes as compared to lower-priced lunchboxes, but there were no differences in the INQs. The contents of energy, protein, fat, saturated fat, and cholesterol significantly differed by brand. Conclusions: Our results indicate that convenience store lunchboxes contain high levels of protein, fat, saturated fat, and sodium. The nutritional contents differed by meal type, price, and store brand, and higher price did not imply higher nutritional quality. We propose the need to educate consumers to check nutrition labels and purchase appropriate lunchboxes. Manufacturers also need to make efforts to reduce the amounts of fat, saturated fat, and sodium, and not provide protein in excess.

• The Korean Journal of Physiology
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• 제19권2호
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• pp.155-160
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• 1985

Dantrolene sodium(DS) is a long acting skeletal muscle relaxant which has been successfully used to control muscle spasticity in patients with various neurological disorders. However, its use is associated with hepatotoxicity. Tissue sulfhydryl group has many important roles for cellular integration and glutathione serves as a substrate for the detoxification metabolism. The purpose of this study were to investigate the effect of DS on tissue sulfhydrl group and glutathione content. Foully albino rats were divided into two groups ; saline treated (control) and DS treated groups. DS dissolved in saline was administered orally. All rats were sacrificed after 7. 14. 21 and 28 days of DS ana saline treatment by dacapitation ana liver was removed for the enzyme preparation. Total and nonprotein sulfhydryl were measured by the method of Sedlak and Lindsay (1968). Total glutathione content was assayed according to the method described by Tietze (1969) and glutathione reductase was assayed according to the method of Racker (1955), The results obtained are summarized as follows : DS administration significantly depressed the total, protein and nonprotein sulfhydryl content in liver. There were significant reduction of both total glutathione content and glutathione reductase activity in liver. On the basis of the above results it may be speculated that the toxicity of DS are well correlated with tissue sulfhydryl content and glutathione reductase activity.

• 동아시아식생활학회지
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• 제20권6호
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• pp.853-862
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• 2010

This study was conducted as a nationwide project in order to obtain data on the content and intake of sodium in school meals. For this purpose, we selected two elementary and two middle schools in Daejeon as well as four elementary schools in the Chungcheong area. We measured the sodium and potassium contents of 842 dishes as well as their intake levels over 154 days. Sodium content was analyzed using an AAS (Atomic Absorption Spectrometer). The average meal intakes of second and fifth grade elementary school students were 244 g/meal and 304 g/meal, respectively. The meal intake of middle school students was 401 g/meal. Boys tended to eat slightly more than did girls, and students tended to eat more in the spring than in the summer. The average sodium intake per meal was 642 mg (570 mg by elementary school students, 1068 mg by middle school students), which was 32.1% of the UL (Tolerable Upper Intake Level, 2,000 mg per day) according to the KDRIs (Dietary Reference Intakes for Koreans). The sodium intake per meal was not significantly differ between boys and girls or between spring and summer (p>0.05). This study provides fundamental data that can be used to establishment a nutrition policy concerning the adequate content of sodium in school meals.

• 한국식품과학회지
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• 제20권5호
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• pp.704-710
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• 1988

본 실험은 여러 가지 보습제 및 방부제(Sodium lactate, Glycerol, Propylene glycol, sorbitol, Mannitol, Sodium benzoate, Potassium sorbate 및 Calcium propionate)처리를 한 중간수분식품 모델의 보수력을 등온흡습 곡선으로 처리하였다. 중간수분식품의 모델은 오징어를 재료로 사용하였고, 제조된 모델식품의 저장성도 검토하였다. 저장요인으로서는 지질산패, 색깔변화 및 곰팡이 번식을 관찰하였다. 보습제의 보수력은 mannitol

• 한국식품영양학회지
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• 제29권5호
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• pp.801-807
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• 2016

In Western countries, kimchi, the Korean traditional fermented cabbage, is considered to be a healthy. However, it is one of the main sources of the high sodium content of the Korean diet. In order to decrease the sodium content, we manufactured a low-sodium kimchi (LK, salinity 1.0%) and 4 additional low-sodium kimchi starters in which each of 4 lactic acid bacteria (Lb. sakei 1, Lb. sakei 2, Lb. palntarum and W. koreensis) were added. The LKL1 to LKL4 samples were prepared by adding 4 single LAB starters, each with an inoculum size of $10^6CFU/g$, when the cabbage was mixed with kimchi sauce. The kimchi starters were fermented at $10^{\circ}C$ until reaching 0.5% acidity, and then stored at $-1.5^{\circ}C$ until reaching 0.75% acidity. The pH and acidity of the starter kimchi changed more rapidly in the early phase of fermentation (up to 0.75% acidity) than control low-sodium kimchi. After the acidity of the kimchi starters reached 0.75% it remained constant. As the fermentation progressed, the total aerobic and lactic acid bacteria concentrations in the kimchi starter with added Lb. sakei 1 were the same as in the control low-sodium kimchi. The low-sodium kimchi fermentation of the kimchi starter with added Lb. palntarum progressed differently due to a difference in acid resistance. The kimchi starter with added Lb. sakei 2 had an overall liking score that was slightly higher than that of the control low-sodium kimchi due to a lower off-flavor.

• 한국식생활문화학회지
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• 제33권6호
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• pp.523-530
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• 2018

The purpose of this study was to develop a technology commercialization strategy by analyzing the consumer preference for low-sodium instant noodle ramen, which has a sodium content of 1,730 mg or less. For the commercialization of such low-sodium ramen, this study examined how much consumers would pay through an analysis of consumer preference by conjoint analysis. This study surveyed 1,004 men and women in their 20 s and 50 s between the ages of 20 and 50 years in Korea using an online survey. If the price of conventional instant noodle ramen is KRW 4,000 (approximately USD 3.57) per 5 packs for one pack of 5 servings in a supermarket, this study surveyed how much more they respondents would pay if the sodium was reduced by 10.0, 20.0, and 30.0%. The study found that if sodium was reduced by 10.0, 20.0, and 30.0%, the respondents would pay as much as KRW 4,180, KRW 4,307, and KRW 4,515, respectively. The attributes of low-sodium instant noodle ramen were examined according to the degree of sodium reduction, vegetable protein ingredients, brand, where to buy and price with each level. The Marginal Willingness To Pay (MWTP) was analyzed as follows. If the sodium content of ramen soup is reduced by 1.0%, the amount that can be paid would be KRW 105.78. Respondents could pay KRW 1,673 more for famous brand ramen than an unknown brand ramen. The study results indicated that when a new product is developed and released, it can be expected to increase sales of the new product by understanding the foreseeable value that consumers could pay for and realize the technological commercialization of the technology.