• Animal Bioscience
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• 제36권9호
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• pp.1376-1383
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• 2023

Objective: We aimed to determine the effects of 12-oxoeicosatetraenoic acid (12-KETE)-induced placenta release on the performance of mother cows (milk yield, ovarian function, and blood plasma biochemical properties). Methods: Experimental treatments were as follows: i) natural delivery including natural placental release (control cows); ii) induced calf delivery with placental retention (RP cows); and iii) induced calf delivery and 12-KETE-induced placental release (KE cows). Delivery in pregnant KE cows was induced with dexamethasone and prostaglandin. These cows were injected with 12-KETE after calf discharge, resulting in the release of the fetal placenta. RP cows were not treated with 12-KETE after inducing delivery, resulting in placental retention. Results: The milk yield in RP cows during the first 50 days after delivery was significantly lower than that in control cows (p<0.05), whereas KE cows exhibited a similar milk yield to that of control cows. The postpartum plasma progesterone levels of control cows increased 14 days after delivery on average; however, its increase was delayed by 10 days in RP cows. Meanwhile, the 12-KETE treatment (KE cows) brought the timing of progesterone increase forward to the normal level (control cows). Among the 20 biochemical parameters examined, the total cholesterol levels in blood plasma 14 days after delivery were lower in RP cows than that in the other two treatment groups (control cows and KE cows) (p<0.05). In addition, the plasma level of haptoglobin tended to be low in cows that discharged their placentas shortly after delivery. Conclusion: These findings indicate that 12-KETE treatment can alleviate the disorder caused by placental retention.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.807-811
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• 2005

This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.580-587
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• 2010

An experiment was conducted to evaluate the effect of dietary water-soluble carbohydrate extract from Renga renga lily (Arthropodium cirratum) and a commercial product, $Frutafit^{{\circledR}}$ (both fructans) on the performance, organ weights, ileal digestibility and gut morphology of male Cobb broiler chickens. There were six treatment groups: a negative control with no supplements, a positive control supplemented with 45 ppm Zn-bacitracin, and four test diets each supplemented with Renga renga lily extract or Frutafit at 5 or 10 g/kg diet. Supplementation with low levels of Renga renga lily extract and Frutafit in the diet did not affect productive parameters, whereas the inclusion of a high level of Frutafit had a negative effect on BWG and FI compared with birds fed the negative control diet. The addition of an antibiotic to the diet significantly improved (p<0.05) the BWG and FCR of broilers. Apparent ileal digestibility of dry matter, starch, protein and fat was not affected (p>0.05) by supplementation with both levels of lily extract and the low level of Frutafit. The apparent ileal digestibility of dry matter, protein and fat was decreased (p<0.05) by the high level of Frutafit. The apparent metabolisable energy (AME) of the diets fed the high level of Frutafit was approximately 0.2 MJ/kg DM lower than that of the negative control group. The addition of Zn-bacitracin increased (p<0.05) the apparent ileal digestibility of fat. The relative weight of the liver was higher (p<0.05) in broilers supplemented with the high level of Frutafit than for negative control birds at 14 and 35 d of age. Feeding Renga renga lily extract or Frutafit had no effect on the gut morphology of birds on d 14 and 35. It can be concluded that dietary inclusion of fructans from the two sources used in this study affected broiler performance differently and in a dose-dependent manner.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1353-1359
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• 2001

The present study was undertaken to assess the influence of dimethyl-sulfoxide plus sucrose solution as a cryoprotectant on the adenosine triphosphate (ATP) content, the ultrastructure and the embryonic development of bovine oocytes matured in vitro. We measured the amount of ATP in cumulus cells enclosed oocytes (CO) or denuded oocytes (DO) equilibrated with or removed from the cryoprotectant (1.5 M DMSO + 0.25 M sucrose + 20% fetal bovine serum in physiological saline). As a result, the ATP contents in both CO and DO, equilibrated with the cryoprotectant, were significantly lower (p<0.05) than that of the each control group. However, ATP content of DO was recovered to the level of the control group ailer removal of the cryoprotectant, but failed to restore for CO. In the observation of the ultrastructure by a transmission electron microscope, all of the mitochondria in the ooplasm of CO and DO equilibrated with the cryoprotectant were swollen with peripherally located cristae following decreased electron density. However, a large proportion of these swollen mitochondria were restored to the normal shape which can be observed usually in the control group after removal from the cryoprotectant. To the contrary, the morphology of many mitochondria of the cumulus cells in CO were not recovered to that of the control group after removal of the cryoprotectant. CO with removed cryoprotectant had significantly lower embryonic development up to the blastocysts stage (p<0.05) after in vitro fertilization compared with that in the control group. These results suggest that the addition and removal of a cryoprotectant has a negative effect for the ATP content of cumulus enclosed oocytes. One of the factor(s) causing the lower embryonic development after removal of cryoprotectant, may be associated with ATP metabolism.

• Asian-Australasian Journal of Animal Sciences
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• 제32권5호
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• pp.607-613
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• 2019

Objective: Intramuscular fat (IMF) content plays an important role in meat quality. Identification of single nucleotide polymorphisms (SNPs) and genes related to pig IMF, especially using pig populations with high IMF content variation, can help to establish novel molecular breeding tools for optimizing IMF in pork and unveil the mechanisms that underlie fat metabolism. Methods: We collected muscle samples of 453 Chinese Lulai black pigs, measured IMF content by Soxhlet petroleum-ether extraction method, and genotyped genome-wide SNPs using GeneSeek Genomic Profiler Porcine HD BeadChip. Then a genome-wide association study was performed using a linear mixed model implemented in the GEMMA software. Results: A total of 43 SNPs were identified to be significantly associated with IMF content by the cutoff p<0.001. Among these significant SNPs, the greatest number of SNPs (n = 19) were detected on Chr.9, and two linkage disequilibrium blocks were formed among them. Additionally, 17 significant SNPs are mapped to previously reported quantitative trait loci (QTLs) of IMF and confirmed previous QTLs studies. Forty-two annotated genes centering these significant SNPs were obtained from Ensembl database. Overrepresentation test of pathways and gene ontology (GO) terms revealed some enriched reactome pathways and GO terms, which mainly involved regulation of basic material transport, energy metabolic process and signaling pathway. Conclusion: These findings improve our understanding of the genetic architecture of IMF content in pork and facilitate the follow-up study of fine-mapping genes that influence fat deposition in muscle.

• Animal Bioscience
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• 제37권4호
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• pp.640-654
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• 2024

Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca²⁺ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.622-629
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• 2010

The effects of dietary supplementation of feather meal (FM) and pyridoxine ($B_6$) on the taurine content of egg yolk and performance of laying hens were investigated. A feeding trial was conducted in nine hundred 31-wk-old $Hy-Line^{\circledR}$ Brown layers over 4 wk. The hens received 6 dietary treatments: Control, FM 3% supplemented diet (FM 3%), FM 3%+$B_6$ supplemented diet (FM 3%+$B_6$), FM 6% supplemented diet (FM 6%), FM 6%+$B_6$ supplemented diet (FM 6%+$B_6$), and synthetic taurine 0.25% supplemented diet (Taurine). Parameters of production were significantly (p<0.05) affected by treatments. The egg production of hens fed FM 3% was the highest and hens fed FM diets were more productive than the Taurine and Control groups. The egg weights of the Taurine group were significantly lower than those of the FM 3% and FM 6% groups, but not significantly different from those of other treatments. The feed intake of the Control group was highest among all groups. The feed conversion ratio of the Control group was higher than in groups receiving other treatments of which FM 6% was the lowest. The broken egg production of the Taurine group was highest, while that of the Control group was lowest among treatments. The taurine content of egg yolk was significantly (p<0.01) increased by supplementation of taurine (64.7%), FM 6%+$B_6$ (57%), FM 3%+$B_6$ (32.1%), and FM 6% (16.6%) over a 4 wk average. Sensory evaluation data of the Taurine group showed the highest score in all of the sensory attributes and those of other treatments were not significantly (p<0.05) different. In conclusion, taurine can be enriched in egg yolk by supplementation of 6% FM and $B_6$, as well as 0.25% synthetic taurine.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.648-654
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• 2004

This study was carried out to assess whether feeding of alcohol-fermented feeds (AFF) affects the nutritional metabolism and growth characteristics of Korean native steers. Ten steers were randomly assigned to one of two treatment groups. The dietary treatments were AFF (50% commercial beef cattle feed+30% alcohol-fermented soybean curd dregs+20% rice straw) and control (80% commercial beef cattle feed+20% rice straw). The change of serum metabolites and growth characteristics were measured every two months during the whole twelve months experimental period and the relationships between serum metabolites and growth characteristics were simultaneously analyzed. Four hours after feeding AFF, serum alcohol concentration reached its peak with a significantly higher value than that after control feeding (11.9 and 4.9 mg/dl, respectively). Serum glucose and inorganic phosphorus (IP) concentrations (63.1 and 8.4 mg/dl, respectively) of steers fed AFF were higher than those (56.6 and 7.0 mg/dl) fed the control diet. In both treatments, the serum glucose concentration rapidly increased when body weight (BW) of the steer reached about 600kg, while IP concentrations were rapidly diminished at that BW. Lower concentrations of both blood urea nitrogen (BUN) and cholesterol were observed in steers fed AFF up to 450 kg of BW. The IP concentration was correlated with concentrations of BUN, cholesterol and glucose in AFF fed cattle but not in the cattle fed control diets. Average daily gain was higher in steers fed AFF than steers fed control, particularly during the growing stage of cattle. These findings indicated a capability of AFF to improve BW gain of Korean native steers by decreased protein degradation as well as increased fat synthesis.

• Asian-Australasian Journal of Animal Sciences
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• 제30권3호
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• pp.370-378
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• 2017

Objective: An experiment was conducted to investigate the effects of a specific mixture of essential oils (MEO), containing thyme, clove and cinnamon EO, on rumen microbial fermentation, nutrient apparent digestibility and blood metabolites in fistulated sheep. Methods: Six sheep fitted with ruminal fistulas were used in a repeated measurement design with two 24-d periods to investigate the effect of adding MEO at 0 (control), 0.8, and 1.6 mL/d on apparent nutrient digestibility, rumen fermentation characteristics, rumen microbial population and blood chemical metabolites. Animals were fed with a 50:50 alfalfa hay:concentrate diet. Results: Ruminal pH, total volatile fatty acids (VFA) concentration, molar proportion of individual VFA, acetate: propionate ratio and methane production were not affected with MEO. Relative to the control, Small peptides plus amino acid nitrogen and large peptides nitrogen concentration in rumen fluid were not affected with MEO supplementation; while, rumen fluid ammonia nitrogen concentration at 0 and 6 h after morning feeding in sheep fed with 1.6 mL/d of MEO was lower (p<0.05) compared to the control and 0.8 mL/d of MEO. At 0 h after morning feeding, ammonia nitrogen concentration was higher (p<0.05) in sheep fed 0.8 mL/d of MEO relative to 1.6 mL/d and control diet. Ruminal protozoa and hyper ammonia producing (HAP) bacteria counts were not affected by addition of MEO in the diet. Relative to the control, no changes were observed in the red and white blood cells, hemoglobin, hematocrit, glucose, beta-hydroxybutyric acid, cholesterol, total protein, albumin, blood urea nitrogen and aspartate aminotransferase and alanine aminotransferase concentration. Apparent total tract digestibility of dry matter, crude proten, organic matter, and neutral detergent fiber were not influenced by MEO supplementation. Conclusion:The results of the present study suggested that supplementation of MEO may have limited effects on apparent nutrient digestibility, ruminal fermentation and protozoa and HAP bacteria count, blood cells and metabolites.

• Asian-Australasian Journal of Animal Sciences
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• 제22권10호
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• pp.1407-1413
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• 2009

An experiment was conducted to investigate the effects of dietary supplementation of feather meal (FM) on the performance of pigs and taurine content in pork. A total of 135 cross-bred (L${\times}$Y${\times}$D) pigs, weighing an average 46 kg, were assigned to five dietary treatments; Control, 3% FM supplemented diet (3% FM), 3% FM and 10 ppm pyridoxin supplemented diet (3% FMpyridoxin), 6% FM supplemented diet (6% FM) and 6% FM and 10 ppm pyridoxine supplemented diet (6% FM-pyridoxin). Each treatment had 3 replications of 9 pigs (4 gilts and 5 barrows) each. Pigs were fed for 11 weeks under a phase feeding program which consisted of grower (0-3 week), grow-finisher (4-8 week) and finisher (9-11 week) periods. During the whole feeding period, ADG and ADFI of pigs fed FM treatments tended to increase in general compared to the control. Feed conversion ratio (feed/gain) of the control was significantly (p<0.05) lower than FM treatments. Taurine content of the control was highest in heart muscle (1,393.8 ppm) followed by liver (647.3 ppm), tenderloin (601.2 ppm), ham (462.4 ppm) and loin (375.8 ppm). Taurine contents of heart, tenderloin, ham and loin were significantly (p<0.05) increased by FM treatments. Taurine content was highest in the 6% FM-pyridoxin treatment. Taurine content of heart, tenderloin, ham and loin on 6% FM-pyridoxin supplementation were increased by 91%, 23%, 27% and 29%, respectively, compared with the control. Sensory tests of the pork showed that there was no consistent response among the treatments. In conclusion, supplementation of FM and pyridoxin to a pig diet can increase taurine content of the pork.