• Mass Spectrometry Letters
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• v.12 no.3
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• pp.106-111
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• 2021

Several pretreatment methods have been developed to reduce the inorganic arsenic, which is known to be highly harmful to humans, among various arsenic species present in hijiki and rice. The pretreatment methods were selected and developed as methods that can be non-harmful even after treatment and easily applied. Hijiki was applied by two methods. One was soaking in water at room temperature for various durations and the other was boiling of it in water for a short period of time. Rice was soaked in water with different rice-to-water ratios for various durations. The most effective method that reduced the inorganic arsenic in hijiki was to repeat parboiling for 5 minutes twice, which led to 79% reduction of the inorganic arsenic in it. In the case of rice, soaking for 24 hours at the ratio of 1:5 (rice:water) resulted in 51% reduction of inorganic arsenic in rice.

• Korean Chemical Engineering Research
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• v.53 no.6
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• pp.682-689
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• 2015

A two-step process was investigated for pretreatment and fractionation of rice straw. The two-step fractionation process involves first, soaking rice straw in aqueous ammonia (SAA) in a batch reactor to recover lignin-rich hydrolysate. This is followed by a second-step treatment in a fixed-bed flow-through column reactor to recover xylo-oligomer-rich hydrolysate. The remaining glucan-rich solid cake is then subjected to an enzymatic process. In the first variant, SAA treatment in the first step dissolves lignin at moderate temperature (60 and $80^{\circ}C$), while in the second step, hot-water treatment is used for xylan removal at higher temperatures ($150{\sim}210^{\circ}C$). Under optimal conditions ($190^{\circ}C$ reaction temperature, 30 min reaction time, 5.0 ml/min flow rate, and 2.3 MPa reaction pressure), the SAA-hot-water fractionation removed 79.2% of the lignin and 63.4% of the xylan. In the second variant, SAA was followed by treatment with dilute sulfuric acid. With this process, optimal treatment conditions for effective fractionation of xylo-oligomer were found to be $80^{\circ}C$, 12 h reaction time, solid-to-liquid ratio of 1:12 in the first step; and 5.0 ml $H_2SO_4/min$, $170^{\circ}C$, and 2.3 MPa in the second step. After this two-step fractionation process, 85.4% lignin removal and 78.9% xylan removal (26.8% xylan recovery) were achieved. Use of the optimized second variant of the two-step fractionation process (SAA and $H_2SO_4$) resulted in enhanced enzymatic digestibility of the treated solid (99% glucan digestibility) with 15 FPU (filter paper unit) of CTec2 (cellulase)/g-glucan of enzyme loading, which was higher than 92% in the two-step fractionation process (SAA and hot-water).

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.240-245
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• 1995

This study was conducted to find out environment conditions in order to increase of growth and yield of tuberlets in pinellia ternata (Thunb.) Breit. The optimal temperature conditions for the growth of tuberlets were good rather temperature fluctuations than constant one and the optimal photoperiod was a 6hr-photoperiod. The growth of tuberlets to the kinds and concentration of growth regulators was good for 0. lppm 2.4- D, 10ppm kinetin and 50ppm gibberellin treatment. In colchicine soaking treatment, the growth of tuberlets was bad, but bulblets formation was good for 0. 05% colchicine treatment for 48hr. -soaking. In colchicine dropping treatment, dry tuber yield was good for 0. 1% colchicine treatment with once dropping for one day.

• The Journal of Advanced Prosthodontics
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• v.12 no.5
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• pp.322-328
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• 2020

PURPOSE. This study evaluated the shear bond strength between 3D printed provisional resin and conventional provisional resin depending on type of conventional provisional resin and different surface treatments of 3D printed resin. MATERIALS AND METHODS. Ninety-six disc-shaped specimens (Ø14 mm × 20 mm thickness) were printed with resin for 3D printing (Nextdent C&B, Vertex-Dental B. V., Soesterberg, Netherlands). After post-processing, the specimens were randomly divided into 8 groups (n=12) according to two types of conventional repair resin (methylmethacrylate and bis-acryl composite) and four different surface treatments: no additional treatment, air abrasion, soaking in methylmethacrylate (MMA) monomer, and soaking in MMA monomer after air abrasion. After surface treatment, each repair resin was bonded in cylindrical shape using a silicone mold. Specimens were stored in 37℃ distilled water for 24 hours. The shear bond strength was measured using a universal testing machine at a crosshead speed of 0.5 mm/min. Failure modes were analyzed by scanning electron microscope. Statistical analysis was done using one-way ANOVA test and Kruskal-Wallis test (α=.05). RESULTS. The group repaired with bis-acryl composite without additional surface treatment showed the highest mean shear bond strength. It was significantly higher than all four groups repaired with methylmethacrylate (P<.05). Additional surface treatments, neither mechanical nor chemical, increased the shear bond strength within methylmethacrylate groups and bis-acryl composite groups (P>.05). Failure mode analysis showed that cohesive failure was most frequent in both methylmethacrylate and bis-acryl composite groups. CONCLUSION. Our results suggest that when repairing 3D printed provisional restoration with conventional provisional resin, repair with bis-acryl composite without additional surface treatment is recommended.

• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.347-354
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• 2016

The present study was performed to investigate the effects of the colchicine concentrations on chromosome doubling for producing of tetraploid plants of Codonopsis lanceolata, and its effect on plant morphology. A total of 180 individuals germinated from 16 treatment groups, were exposed to various concentrations (0.05-1.0% w/v) of colchicine for different soaking duration (3-24 hour). The highest numbers of tetraploid plants (3) were observed from the lowest concentration of colchicine (0.05%), and one (1) tetraploid plant was obtained from the 0.5% concentration group with a 6 hour treatment. However, no tetraploid individual was observed in any other treatment groups. The plant height of the diploid (18.1 ㎝) was slightly shorter than that of the tetraploid (13.4 ㎝). The fresh weight of the main root in the diploid (0.5 g) was four-fold higher than the tetraploid (2.2 g). The colchicine-treated plant regeneration rate in C. lanceolata was decreased when the plants were subjected to high concentration of colchicine. In particular, the highest number of tetraploid plants (5 and 3) was obtained from the lower concentration (0.05% and 0.1%) of colchicine for 6-hour treatment, which were a higher rate (29.4% and 30%) of regenerated tetraploid plants than other regenerated plants. As in the seed treatment result, the plant height of the diploid was significantly higher (10.4 ㎝) than tetraploid. The higher morphological changes were observed comparatively from tetraploid plants than the diploid.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.251-257
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• 2001

This experiment was conducted to investigate the effect of chitosan on the growth and yield of fall-planted potatoes. The research was carried out in fall-planted potatoes from August 2000 to Aprial 2001 at the height of 250m above sea level in Jeju Island. Soaking for 30 min. of seed potatoes in 200 times diluted solution of 3% liquid chitosan shortened days to emergence by 4 to 6 days. Stem length, number of stems and number of stolons per plant were 56.5 cm, 4.3, and 19.0, respectively, when seed potatoes were soaked for 30 min. in 200 times diluted solution of 3% liquid chitossan. Total potato yield in this treatment reached 2,963 kg per 10a and was significantly greater than in other treatments. Yield of marketable tubers (greater than 30g) per 10a for treatment of seed potatoes soaking in 200 times diluted solution of 3% liquid chitosan and for treatment of foliage spray with 200 times diluted solution of 3% liquid chitosan plus chitosna power (10 kg per 10a) miximg with soil was 2,761 kg and 2,628 kg, respectively. Contents of Mg, Fe and B were the greatest in the treatment of 30 min. soaking of seed potatoes in 200 times diluted solution of 3% liquid chitosanm. The increased contents of these elements are considered to have caused yield increase as a result of increased chlorophyll content for photosynthesis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.42-51
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• 2010

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.123-137
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• 2015

This study was carried out to investigate the effect of pre-germination soaking on germination in 90 Korean soybean varieties and identification of protein in seeds of 7 soybean varieties. The results obtained that germination rate of soybean seeds was decreased as amount of soaking water and soaking duration in number of days. Difference in germination rate of soybean seeds was significant at three days soaking with water volume of 90 ml. Water absorption of seeds was rapidly increased during the first 6 hours, followed by slow increase until 24 hours and then decreased 24 to 48 hours after soaking soybean varieties for bean sprout soaked the lowest amount of water, while soybean varieties for cooking with rice showed the lowest seed water content. Dissolved oxygen (DO) in soaking water was rapidly decreased during the first 3 hours after soaking, and then slowly decreased. Soybean varieties for vegetable and early maturity showed the lowest DO during early soaking periods, but showed higher DO after 24 hours than other groups of soybean varieties. Electrical conductivity and Total Dissolved Solid (TDS) were increased as number of soaking days increased. Soybean varieties for vegetable and early maturity showed the highest electrical conductivity and TDS, followed by those for sauce and paste or cooking with rice, while showed the lowest electrical conductivity and TDS, varieties for bean sprout. Among 90 Korean soybean varieties, varieties which showed the highest germination rate were Jangsu-kong for sauce and paste, Sobaegnamul-kong for bean sprout, Seonheuk-kong for cooking with rice, Seunnokkong for vegetable and early maturity. On the ather hand varieties which showed the lowest germination rate were Iksan and Songhak-kong for sauce and paste, Pangsa-kong for bean sprout, Jinyeul-kong for cooking with rice, Sinlok-kong for vegetable and early maturity. Germination rates of soybean seeds were higher when electrical conductivity, TDS and water absorption of seeds were lower. There were negative correlations between electrical conductivity, TDS and water absorption of seeds and germination rate, while there were positive correlations among electrical conductivity, TDS and soybean seed weight.

• Journal of Korean Society of Forest Science
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• v.44 no.1
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• pp.26-35
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• 1979

Factors affecting the survival of out planted Cryptomena and Japaneses Cypress seedlings. (I) - Especially on the drying and water soaking duration ­ There are often the cases of poor survival percentage for the economic forest establishments of Cryplomeria japonica D. Don. and Chamaecyparis obtusa End. This study was carried out to investigate the survival factors of both 1-1 seedlings and Cryptomena cutting Seedlings by room drying conditions, day drying conditions, OED green water soaking treatment and drying duration at the forest nursery of Chonnam National University from February to September. 1979. The results are as follows: 1. From 11 a.m. to 3 p.m. fluctuation in then moisture contents(MC) rapidly decreased and M.C. fluctuation in indoor until 5 p.m. showed the same results with the amount of an hour fluctuation in outdoor. 2. M.C. fluctuations between OED green and non-treatment of these seedlings were silghtly different. 3. The survival percentage of OED green and non-treatment of seedlings were highly significant in the room drying conditions and the day drying conditions respectively The effects of water soaking treatment on rooting was negligible. The survival percentage of seedlings of OED green was higher than that of non-treatment. 4. Although survival percentage of Japanese Cypress was almost in accord with Cryptomena, the power of resistance to drying was lower m Japanese Cypress than m Cryptomeria. 5. Compared with Cryptomena seedlings the survival percentage of Cryptomeria cutting seedlings was higher and not influenced at all in case of being drying for two hours; but it was highly significant in interactions between the drying time and the water soaking time. 6. When Cryptomena and Japanese Cypress seedling become drying in indoor for four days, the survival percentage remarkably reduced after two days and also was highly significant among numbers of drying days.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.1
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• pp.9-15
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• 2007

The study was conducted to find out the harvesting time, storage duration, cold treatment and sterilization on seed germination of Miscanthus sinensis. The optimum harvesting time of the Miscanthus seed in native to Jeju island was December. And the seed germination passible 3 years after harvesting. When the seeds had cold treatment, seed germination nate was 74% rather than 63% of non-treatment. For seed sterilization treatment, soaking in solution of Benomyl 7% during from 3 hours to 12 hours germinated 9% over. But the non-treatment on 24 hours treatment reduced the seed germination of Miscanthus sinensis.