• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1591-1598
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• 2004

To isolate the $\beta$-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated strains, the strain of KNOUC112 produced the highest amounts of $\beta$-galactosidase at 40 h incubation time (0.013 unit). This strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment producing. Optimum growth was at 70-72$^{\circ}C$, pH 7.0-7.2, and it could grow in the presence of 3% NaCl. The main fatty acids of cell components were iso-15:0 (30.26%), and iso-17:0 (31.31%). Based on morphological and biochemical properties and fatty acid composition, the strain could be identified as genus Thermus, and finally as Thermus thermophilus by phylogenetic analysis based on 16S rRNA sequence. So the strain is designated as Thermus thermophilus KNOUC112. A gene from Thermus thermophilus KNOUC112 encoding $\beta$-galactosidase was amplified by PCR using redundancy primers prepared based on the structure of $\beta$-galactosidase gene of Thermus sp. A4 and Thermus sp. strain T2, cloned and expressed in E. coli JM109 DE3. The gene of Thermus thermophilus KNOUC112 $\beta$-galactosidase(KNOUC112$\beta$-gal) consisted of a 1,938 bp open reading frame, encoding a protein of 73 kDa that was composed of 645 amino acids. KNOUC112$\beta$-gal was expressed as dimer and trimer in E. coli JM109 (DE3) via pET-5b.

• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.49-54
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• 2008

The gene encoding human pancreatic pro-carboxypeptidase B (CPB) was cloned and fused to Saccharomyces cerevisiae mating factor alpha-1 secretion signal $(MF{\alpha}1)$, in which the transcription of $MF{\alpha}1$-pro-CPB was under the control of GAL10 promoter. The constructed plasmid $pY{\alpha}$-hproCPB(7.72 kb) was transformed into S. cerevisiae 2805. The recombinant human pro-CPB (hproCPB) was successfully expressed in S. cerevisiae after induction of galactose, and could be secreted into the culture medium. By analyses of SDS-PAGE and western blotting, the molecular weight of the purified hproCPB was estimated to be a 45.9kDa. The activity of extracellular hCPB after removal of pro-region by trypsin treatment reached about 10.16 unit/ml at batch culture of S. cerevisiae $2805/pY{\alpha}$-hproCPB for 60 h. Also, the Km value of partially purified recombinant hCPB is about 0.43 mM.

• Korean Journal of Head & Neck Oncology
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• v.5 no.1
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• pp.21-25
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• 1989

It is frequently difficult to assess the presence of malignancy in patients with parotid tumor, because of unreliable diagnostic tools and riskful preoperative biopsy, In our case, a parotid adenocarcinoma and pleomorphic adenoma was revealed on permanent histologic section, which was made a impression as pleomorphic adenoma on preoperative Tru-Cut biopsy and intraoperative frozen section, So, the most accurate diagnosis of the parotid malignancy is a complete removal of tumor tissue, accompanied with permanent histologic section.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.9-19
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• 2003

Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. Many efforts have made characterization of GGTA1 in structure and function, improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTAl gene by Missouri group in 2002 To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. Of other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.

• Journal of the Korean Society of Costume
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• v.21
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• pp.19-30
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• 1993

There are record that ancient Korean WOOl fabrics male from sheep, camel, rabbit. bear, horse. calile, raccoon dog, fox and so on. Wool fiber fabriher were also reported Kye, Gal, China, Tap Dung, Kuyu anul ram. These wool fabrics wre named amongling to their fineness and weaving method in the fabric. Technology of wool fabric weaving was developed and from Sam Kuk dynasties to to Koryo Dynasty wool fabrics were reported to weave and trade to China, Japan and Arabia. These fabrics were mainly used as clothes, rugs or blankets. In Koryo Dynasty, especially Kye Kurn was used for trading goods to China. There is another record that two thousand sheep, camels offord Koryo by Yo and Kum Dynasty. In this study, the characteristics of Korean wool fabrics will be disscussed from the literature survey of the relevant references.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.9-19
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• 2003

Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. ry1any efforts have made characterization of GGTA1 in structure and function. improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTA1 gene by Missouri group in 2002. To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. If other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.

• Journal of the Korean Society of Safety
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• v.24 no.3
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• pp.25-31
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• 2009

Safety engineers should be reasonably assigned to construction sites in order to prevent accidents specially for large-scaled projects. More engineering-oriented safety engineers should be assigned to those large-scaled projects because accident causes are found to be due to more technical aspects rather than general safety problems. more experienced engineers should be assigned to larger scaled projects for covering more technical aspects. This one is analyzed by the existing accident results. This study is to find out some formulae to calculate how many safety engineers should be assigned to construction sites, by amounts, by project kinds, by project scales, by engineer grades. So that construction contractor can assign them to their site economically.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2589-2593
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• 2008

In a fuel cell vehicle using polymer electrolyte membrane fuel cell(PEMFC), hydrogen is over-supplied to gain higher stack efficiency. So it is needed considering fuel efficiency to re-circulate hydrogen which is not reacted in stack. And to re-circulate hydrogen, a blower or an ejector is used. Ejector re-circulation system has several merits compared with blower system, for example no parasite energy, simple structure and no lubrication system. But the secondary flow of an ejector in fuel cell vehicle, has high humidity because of crossover problem in stack. Therefore in this paper, ejector is designed by 1-D modeling and CFD with the primary and secondary flow of hydrogen. And the ejector which has the primary and secondary flow of air, is designed to have the same Reynolds number and Mach number at the nozzle exit as the hydrogen ejector's. And this air ejector is tested while the humidity of the secondary flow is varied.

• Herbal Formula Science
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• v.15 no.2
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• pp.21-33
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• 2007

At this moment, because there're lots of necessity to have an attention on diabetus mellitus (DM), in this article searched over all the Korean oriental medical academic journals about DM from 2003 to 2007, Method: Through data base system that Daegu Haany University affiliated information center & Korean Studies Information Center manage, selected 60 articles worthy to look up that are searched by the keyword 'DM' & '消渴'. Result and Conclusion: 5 articles of observing 'DM' & '消渴' itself academically, 29 articles of experimental study, 8 articles of clinical research study and 18 articles of case report. For experimental study, mostly it were concerning type II DM modeled rat medicated herb and its effect, so there were only a few type I experimental study. For observing articles, generally the causes, pathology, treatments, acupuncture methods and medication et al., variable studies were done. In clinical research, diverse categories were subject, which is to prove the efficacy of medication, acupuncture, physical therapy and diet supplements. And in case reports, focused not only on improvement in blood sugar level but also its complications.

• Membrane Journal
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• v.18 no.2
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• pp.109-115
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• 2008

To remove total dissolved solid (TDS) from wastewater, a carbon membrane system was prepared, using carbon membranes made from conductive activated carbon and poly(vinylidene fluoride) (PVDF). Using 100 ppm aqueous solutions of NaCl, $Na_2SO_4,\;MgCl_2,\;MgSO_4$, the basic properties of the carbon membranes used were studied. For the treatment of the real dye wastewater supplied from Kyungin Corp., a pilot scale carbon membrane system was also prepared, which was consisted of 240 plies of carbon membranes of $20cm{\times}20cm$ (length${\times}$width). Using the real wastewater with different TDS such as 941, 2050, 2810, 3830, 4960, 6030 ppm, prepared by the dilution of the original wastewater with pure water, the performance of the pilot scale carbon membrane system was studied. The effect of the operational conditions was studied.