• Smart Structures and Systems
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• v.9 no.3
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• pp.287-301
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• 2012

It is well known that overloaded vehicles may cause severe damages to bridges, and how to estimate and evaluate the status of the overloaded vehicles passing through bridges become a challenging problem. Therefore, based on the monitored strain data from a structural health monitoring system (SHM) installed on a bridge, a method is recommended to identify and analyze the probability of overloaded vehicles. Overloaded vehicle loads can cause abnormity in the monitored strains, though the abnormal strains may be small in a concrete continuous rigid frame bridge. Firstly, the abnormal strains are identified from the abundant strains in time sequence by taking the advantage of wavelet transform in abnormal signal identification; secondly, the abnormal strains induced by heavy vehicles are picked up by the comparison between the identified abnormal strains and the strain threshold gotten by finite element analysis of the normal heavy vehicle; finally, according to the determined abnormal strains induced by overloaded vehicles, the statistics of the overloaded vehicles passing through the bridge are summarized and the whole probability of the overloaded vehicles is analyzed. The research shows the feasibility of using the monitored strains from a long-term SHM to identify the information of overloaded vehicles passing through a bridge, which can help the traffic department to master the heavy truck information and do the damage analysis of bridges further.

• The Journal of the Korean Society for Microbiology
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• v.21 no.4
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• pp.461-471
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• 1986

Shigella strains isloated in the Teagu area during the period from 1973 to 1985 were studied for species distribution, drug resistance, and R plasmids. Approximately 1,200 strains were isolated during this period, and most of them were classified into Shigella flexneri, S. sonnei occupied less than 20%, and S. dysenteriae and S. boydii were very rarely isolated. More than 95% of them were resistant to one or more of these drugs; chloramphenicol (Cm), tetracycline (Tc), streptomycin (Sm), sulfisomidine (Su), ampicillin (Ap), and trimethoprim (Tp). Strains resistant to kanamycin, nalidixic acid (Na), and rifampin (Rf) were rare, and no strain was resistant to cephaloridine, gentamicin, and amikacin. Approximately half of the isolates were resistant to drugs in 1973, but the rate of resistant strains increased to more than 95% from 1977. Strains resistant to the four drugs (Cm, Tc, Sm, and Su) occupied the majority of resistant strains until 1977, but the most prevalent multiplicity of drug resistance increased to six drugs (Cm, Tc, Sm, Su, Ap, and Tp) from 1978 with the marked increase of Ap- and Tp-resistant strains. Approximately 75% of them transferred resistance to Escherichia coli by conjugation, and the resistance was considered to be mediated by R plasmids. Almost all of them transferred the complete patterns of resistance to drugs except Na and Rf. However, among some strains of recent isolates, small numbers of segregants of transferred resistance were observed. The R plasmids in Shigella were mostly classified into Inc FII, and only small numbers into Inc B. Segregants were in most cases unclassified.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.940-951
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• 2009

A comparative study on dynamic and static measurement of initial stiffness was conducted. Because soil stiffness decreases even at very small strains, the initial stiffness has been measured by dynamic tests using shear wave velocity measurement. On the other hand, due to the advance of local strain measurement, the triaxial testing device is capable of measuring the static initial stiffness. It has been known that initial stiffness measured by static triaxial tests is generally lower than that measured by dynamic tests possibly due to the limitation of static measurement of displacement at very small strains. This study presents experimental results indicating that the elastic shear moduli could be the same both in dynamic and static measurements owing to the soil anisotropy induced by anisotropic stresses.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.759-768
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• 1994

E coli strains isolated from pigs were investigated with respect to antimicrobial resistances and prevalence of R-plasmids. Also determined were properties of R-plasmids by plasmid conjugation, curing and southern hybridization using gene probes. All of 400 E coli strains were resistant to CL and SU, and 0.3% to 96.8% of the strains were resistant to most antimicrobials such as TC, PG, AM, SM, CP, GM, EM, NM, etc, while all strains were sensitive to AK. All strains were also multiply resistant to three to twelve antimicrobials. The resistances to PG, SM, TC, AM, CP, SU and ST were transferable and supposed to be mediated by R-plasmids which were opportunistic for transposition into chromosome. Plasmids bigger in size than chromosomal DNA were considered as R-plasmids and most plasmids in small size (<4Kb) proved as cryptic plasmids or nonconjugative R-plasmids. In a strain(No 99), AM resistant property was determined from both chromosomal DNA and R-plasmid DNA which is bigger in size than chromosome.

• Journal of Microbiology and Biotechnology
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• v.19 no.8
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• pp.749-759
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• 2009

A survey of Bacillus thuringiensis (Berliner) strains isolated from Spanish citrus orchards has been performed, and the strains were tested for insecticidal activity against the Mediterranean fruit fly Ceratitis capitata (Wiedemann), a key citrus pest in Spain. From a total of 150 environmental samples, 376 isolates were selected, recording a total B. thuringiensis index of 0.52. The collection was characterized by means of phase-contrast microscopy, SDS-PAGE, and PCR analysis with primer pairs detecting toxin genes cry1, cry2, cry3, cry4, cry5, cry7, cry8, cry9, cry10, cry11, cry12, cry14, cry17, cry19, cry21, cry27, cry39, cry44, cyt1, and cyt2. Diverse crystal inclusion morphologies were identified: bipyramidal (45%), round (40%), adhered to the spore (7%), small (5%), and irregular (3%). SDS-PAGE of spore-crystal preparations revealed 39 different electrophoresis patterns. All primer pairs used in PCR tests gave positive amplifications in strains of our collection, except for primers for detection of cry3, cry19, cry39, or cry44 genes. Strains containing cry1, cry2, cry4, and cry27 genes were the most abundant (48.7%, 46%, 11.2%, and 8.2% of the strains, respectively). Ten different genetic profiles were found, although a total of 109 strains did not amplify with the set of primers used. Screening for toxicity against C. capitata adults was performed using both spore-crystal and soluble fractions. Mortality levels were less than 30%. We have developed a large and diverse B. thuringiensis strain collection with huge potential to control several agricultural pests; however, further research is needed to find out Bt strains active against C. capitata.

• Journal of the Korean Geotechnical Society
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• v.16 no.4
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• pp.95-105
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• 2000

Anisotropy of stiffiness, from extremely small strains to post-failure strains, of isotropically consolidated air-pulviated sands in plane strain compression was studied by using the newly developed instrumentation for small strain measurements. Seven types of sand of the world-wide origins were tested, which have been extensively used for research purposes. Stress-strain relationships for a wide range of strain from about 0.0001% to 10% were obtained with measuring axial and lateral strains locally free from the effects of bedding and membrane penetration errors at the specimen boundaries. It was found that the maximum shear modulus Gmax was irrespective of the angle $\delta$of the $\sigma$1 direction relative to the bedding plane. However, the normalized Gmax was varied with the types of sand. Furthermore, the dependency of the strain and stress level on the stiffness increased as decreased.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.2
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• pp.183-186
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• 2004

Recently, we have developed an easy, simple and convenient circular DNA cloning system named plasmid capture system (PCS). To investigate usefulness of PCS in cloning of plasmids from Bacillus thuringiensis strains, PCS donors, pPCS-S and pPCS-L were applied to clone plasmids of B. thuringiensis subsp. israelensis by in vitro transposition using 4{TnsABC^*}$ transposase. In result, 3 small plasmids were cloned, and these were consistent with pTX14-1, pTX14-2 and pTX14-3 reported previously from B. thuringiensis subsp. israelensis. Therefore, the PCS can be successfully applied to clone small plasmids from B. thuringiensis strains.

• Parasites, Hosts and Diseases
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• v.37 no.3
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• pp.181-188
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• 1999

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18s rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18s riboprinting. Acanthamoeba griffini of morhological group 2 branched between pathogenic (A. culbertsoni A-1 and A. healyi OC-3A) and nonpathogenic (A.palestinensis Reich, A. pustulosa GE-3a, A. royreba Oak Ridge, and A lenticulata PD2S) strains of morphological group 3. Among the remaining isolates of morphological group 2, the Chang strain had the identical mitochondrial riboprints as the type strain of A. hatchetti. AA2 and AA1, the type strains of A. divionensis and A. paradivionensis, respectively, had the identical riboprints as A. quina Vil3 and A. castellanii Ma. Although the branching orders of A. castellanii Neff, A. polyphaga P23, A. triangularis SH621, and A. lugdunensis L3a were different from those in 18S riboprinting analysis, the results obtained from this study generally coincided well with those from 18S riboprinting. Mitochondrial riboprinting may have an advantage over nuclear 18S rDNA riboprinting beacuse the mt SSU rDNAs do not seem to have introns that are found in the 18S genes of Acanthamoeba and that distort phylogenetic analyses.

• Journal of the Korea institute for structural maintenance and inspection
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• v.9 no.1
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• pp.135-145
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• 2005

Geotechnical design routinely requires that in-situ strength, stiffness of the ground be determined. In the working stress conditions, the strain level in a ground experienced by existing structures and during construction is less than about 0.1%~1%. In order to analyze the deformational behavior accurately, the in-situ testing technique which provides the reliable deformational characteristics at small strains, needs to be developed. Cone pressuremeter tests were performed on the western off-shore region of korea, and analyzed using cavity expansion theory and curve fitting technique to obtain the shear modulus at small strain level of $10^{-1}%$. The value of $E_u/S_u$ ratio for the marine clay shows about 589 at the small strain. However the value of $E_u/S_u$ estimated by lab tests are much smaller values ranged from 81 to 91. It is indicated that the curve fitting technique from CPM tests results can be used to obtain the shear modulus at small strain.

• Korean journal of applied entomology
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• v.14 no.2 s.23
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• pp.53-58
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• 1975

The local variabilities of resistance to Malathion and NAC (Sevin) were studied during 1973 with the strains of small brown planthopper (Laodelphax striatellus Fallen) collected from Jinjoo, Sunsan, Milyang, Kwangjoo and Iri districts of southern part of Korea. The susceptible lab strain used for this study has been reared successively in the laboratory without exposure to any insecticide since 1970. The insecticides were applied topically in acetone to the thoracic sternum of the adult insects. $LD_{50}$ values of Malathion to the insect were varied with the locality of the planthoppers. Relatively high levels of resistance to Malathion were observed in the strains of Jinjoo and Suntan; showing 27.7 times in female and 24.9 times in male for Jinjoo stram and 18.4 times in female and 13.8 times in male for Sunsan strain as compared with the susceptible lab strain, and in other strains of Milyang, Kwangjoo and Iri the levels of resistance to Malathion were still less than 5 times. However, there was no any specific difference among the strains in susceptibility to NAC. The malathion-resistant strain of the small brown planthopper seemed to be still localized in some regions of· Korea. The susceptibility of the planthoppers to Malathion was varied with the sex; $LD_{50}$ values based on the individual (ug/insect) were higher in female than in male, while the values based on the body weight (ug/g) conversely higher in male than in female.