Browse > Article

Cloning of Small Plasmids from Bacillus thuringiensis Subsp. israelensis Using Plasmid Capture System  

Choi, Jae Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Roh, Jong Yul (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Li, Ming Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Shim, Hee Jin (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Kang, Joong Nam (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Woo, Soo Dong (Department of Agricultural Biology, Chungbuk National University)
Jin, Byung Rae (College of Natural Resources and Life Science, Dong-A University)
Je, Yeon Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.9, no.2, 2004 , pp. 183-186 More about this Journal
Abstract
Recently, we have developed an easy, simple and convenient circular DNA cloning system named plasmid capture system (PCS). To investigate usefulness of PCS in cloning of plasmids from Bacillus thuringiensis strains, PCS donors, pPCS-S and pPCS-L were applied to clone plasmids of B. thuringiensis subsp. israelensis by in vitro transposition using 4{TnsABC^*}$ transposase. In result, 3 small plasmids were cloned, and these were consistent with pTX14-1, pTX14-2 and pTX14-3 reported previously from B. thuringiensis subsp. israelensis. Therefore, the PCS can be successfully applied to clone small plasmids from B. thuringiensis strains.
Keywords
Bacillus thuringiensis; Plasmid; Plasmid capture system;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Andrup, L., J. Damgaard and K. Wassermann (1993) Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation. J. Bacteriol. 175, 6530-6536   DOI
2 Glare, T. R. and M. O'Callaghan (2000) Bacillus thuringiensis: Biology, Ecology and Safety. John Wiley & Sons, Chichester.
3 Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. BioI. 166, 557-580   DOI
4 Kanda, K., Y. Tan and K. Aizawa (1989) A novel phage genome integrated into a plasmid in Bacillus thuringiensis strain AF1O1. J. Gen. Microbiol. 135, 3035-3041
5 Lereclus, D. and O. Arantes (1992) spbA locus ensures the segregational stability of pHT1030, a novel type of Gram-positive replicon. Mol. Microbiol. 6, 35-46   DOI   ScienceOn
6 Madsen, S. M., L. Andrup and L. Boe (1993) Fine mapping and DNA sequence of replication functions of Bacillus thuringiensis plasmid pTX14-3. Plasmid 30, 119-130   DOI   ScienceOn
7 Nickerson, K. W., G. St Julian and L. A. Bulla Jr. (1974) Physiology of spore forming bacteria associated with insects: Radiorespirometric survey of carbohydrate metabolism in the 12 serotypes of Bacillus thuringiensis. Appl. Microbiol. 28, 129-132
8 Schnepf, E., N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigier and D. H. Dean (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. BioI. Rev. 62, 775-806
9 Berry, C., S. ONeil, E. Ben-Dov, A. F. Jones, L. Murphy, M. A. Quail, M. T. G. Holden, D. Harris, A. Zaritsky and J. Parkhill (2002) Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 68, 5082-5095   DOI   ScienceOn
10 Battisti, L., B. D. Green and C. B. Thorne (1985) Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. J. Bacteriol. 162, 543-550
11 Kronstad, J. W., H. E. Schnepf and H. R. Whiteley (1983) Diversity of location for Bacillus thuringiensis crystal protein genes. J. Bacteriol. 154, 419-428
12 Hoflack, L., J Seurinck and J. Mahillon (1997) Nucleotide sequence and characterization of the cryptic Bacillus thuringiensis plasmid pGI3 reveal a new family of rolling circle replicons. J. Bacteriol. 179, 5000-5008   DOI
13 Jensen, G. B., A. Wilcks, S. S. Petersen, J. Damgaard, J. A. Baum and L. Andrup (1995) The genetic basis of the aggregation system in Bacillus thuringiensis subsp. israelensis is located on the large conjugative plasmid pXO16. J. Bacteriol. 177, 2914-2917   DOI
14 Kim, U. -J., H. Shizuya, P. J. de Jong, B. Birren and M. I. Simon (1992) Stable propagation of cosmid sized human DNA inserts in an F factor based vector. Nucleic Acid Res. 11, 1083-1085
15 Ozawa, K. and H. Iwahana (1986) Involvement of a transmissible plasmid in heat-stable exotoxin and delta-endotoxin in Bacillus thuringiensis subspecies darmstadiensis. Curr. Microbiol. 13, 337-340   DOI
16 Jensen, G. B., L. Andrup, A. Wilcks, L. Smidt and O. M. Poulsen (1996) The aggregation-mediated conjugation system of Bacillus thuringiensis subsp. israelensis: host range and kinetics. Curr. Microbiol. 33, 228-236   DOI   ScienceOn
17 Andrup, L., J. Damgaard, K. Wassermann, L. Boe, S. M. Madsen and F. G. Hansen (1994) Complete nucleotide sequence of the Bacillus thuringiensis subsp. israelensis plasmid pTX14-3 and its correlation with biological properties. Plasmid 31, 72-88   DOI   ScienceOn
18 Boe, L., T. T. Nielsen, S. M. Madsen, L. Andrup and G. Bolander (1991) Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis. Plasmid 25, 190-197   DOI   ScienceOn
19 Andrup, L., G. B. Jensen, A. Wilcks, L. Smidt, L. Hoflack and J. Mahillon (2003) The patchwork nature of rolling-circle plasmid: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid 49, 205-232   DOI   ScienceOn
20 Wilcks, A., N. Jayaswal, D. Lereclus and L. Andrup (1998) Characterization of plasmid pAW63: a second self-transmissible plasmid in Bacillus thuringiensis subspecies kurstaki HD-73. Microbiology 144, 1263-1270   DOI   ScienceOn
21 McDowell, D. G. and N. H. Mann (1991) Characterization and sequence analysis of a small plasmid from Bacillus thuringiensis var. kurstaki HD1-DIPEL. Plasmid 25, 113-120   DOI   ScienceOn
22 Lopez-Meza, J. E., J. E. Barboza-Corona, M. C. D. Rincon-Castro and J. E. Ibarra (2003) Sequencing and characterization of plasmid pUIBI-1 from Bacillus thuringiensis serovar entomocidus LBIT-113. Curr. Microbiol. 47, 395-399
23 Mahillon, J. and J. Seurinck (1988) Complete nucleotide sequence of pGI2 a Bacillus thuringiensis plasmid containing Tn4430. Nucleic Acid Res. 16, 11827-11829   DOI   ScienceOn
24 Andrup, L., H. H. Bendixen and G. B. Jensen (1995) Mobilization of Bacillus thuringiensis plasmid pTX14-3. Plasmid 33, 159-167   DOI   ScienceOn
25 Lereclus, D., M. M. Lecadet, J. Ribier and R. Dedonder (1982) Molecular relationships among plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains. Mol. Gen. Genet. 186, 391-392   DOI   ScienceOn
26 Ben-Dov, E., G. Nissan, N. Pelleg, R. Manasherob, S. Boussiba and A. Zaritsky (1999) Refind, circular restriction map of the Bacillus thuringiensis subsp. israelensis plasmid carrying the mosquito larvicidal genes. Plasmid 42, 186-191   DOI   ScienceOn
27 Khan, S. (1997) Rolling-circle replication of bacterial plasmids. Microbiol. Mol. Biol. Rev. 61, 442-455
28 Khan, S. (2000) Plasmid rolling-circle replication: recent developments. Mol. Microbiol. 37,477-484   DOI   PUBMED   ScienceOn
29 Carlton, B. C. and J. M. Gonzales Jr. (1985) Plasmid and deltaendotoxin production in different subspecies of Bacillus thuringiensis; in Molecular Biology of Microbial Differentiation. Hoch, J. A. and P. Seltow (eds.), pp. 246-252, American Society for Microbiology, Washington, D.C.
30 Verheust, C., G. Jensen and J. Mahillon (2003) pGlL01, a linear tectiviral plasmid prophage originating from Bacillus thuringiensis serovar israelensis. Microbiology 149, 2083-2092   DOI   ScienceOn
31 Reddy, A., L. Battisti and C. B. Thorne (1987) Identification of self-transmissible plasmids in four Bacillus thuringiensis subspecies. J. Bacteriol. 169, 5263-5270   DOI
32 Shizuya, H., B. Birren, U. -J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M. Simon (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797   DOI   ScienceOn
33 Mahillon, J., R. Rezsohazy, B. Hallet and J. Delcour (1994) IS231 and other Bacillus thuringiensis transposable elements: a review. Genetica 93, 13-26   DOI   PUBMED