• 한국식품영양학회지
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• 제1권1호
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• pp.33-40
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• 1988

A Study was conducted to investigate the effect of feeding Lactobacillus casei YS on the growing performance and gastrointestinal flora of the suckling piglets, which were delivered from 2 heads of three-way crossbred(Landrace$\times$Large White$\times$Duroc) pigs, for 4 weeks. The results from the present study was summarized as follows. Average body weight gains of feeding group was slightly better than that of control group and diarrhea was prevented by successive 7 days feeding. Population levels of lactic acid bacteria were maintained about 107 colony forming unit(cfu) per gram of the contents in both feeding and control group at upper parts of small intestine. In this part, coliform count was greatly reduced in (ceding group but not in control group. pH values of the intestinal contents were gradually decreased especially at the upper part of alimentary track of feeding group. Among lactic acid bacteria, L. salivarius, L. cases and L. fermentum were found most predominant strains in feeding group, Wheareas L. salivarius, L. acidophilus and L. cunts in control group. In the other hand, Escherichia coli recovered from scouring pigs were resistant to the drug such as streptomycin, ampicillin and sensitive to gentamycin and neomycin in vitro test.

• Journal of Microbiology
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• 제33권2호
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• pp.95-102
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• 1995

A probability identification matrix of acidophilic Streptomyces was constructed. The phenetic data of the strains were derived from numerical classification described by Seong et al. The minimum number of diagnostic characters was determined using computer programs for calculation of different separation indices. The resulting matrix consisted of 25 clusters versus 53 characters. Theoretical evaluation of this matrix was achieved by estimating the chuster overlap and the identification scores for the Hypothetical Median Organisms (HMO) and for the representatives of each cluster. Cluster overlap was found to be relatively small. Identification scores for the HMO and the randomly selected representatives of each cluster were satisfactory. The matrix was assessed practically by applying the matrix to the identification of unknown isolates. Of the unknown isolates, 71.9% were clearly identified to one of eight clusters. The numerical classification data was also used to design a selective isolation medium for antibiotic-producing organisms. Four chemical substances including 2 antibiotics were determined by the DLACHAR program as diagnostic for the isolation of target organisms which have antimicrobial activity against Micrococcus luteus. It was possible to detect the increased rate of selective isolation on the synthesized medium. Theresults show that the numerical phenetic data can be applied to a variety of purposes, such as construction of identification matrix and selective isolation medium for acidophilic antinomycetes.

• Child Health Nursing Research
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• 제3권1호
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• pp.42-51
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• 1997

Although a family-centered approach to health care for developmentally disabled children has been advocated, existing systems of care have not adequately addressed the support needs of the family system and the essential role that parents play in the daily care of these children. The overall purpose of this research is to examine family system adaptation to the care of a developmetally disabled child using the Resiliency Model of Family Stress, Adjustement, and Adaptation Framework. Relationships among family system demands (stressors, strains, transitions, child's illness factors) and family system strengths and capabilities(resource of social support) were examined to determine their separate and combined contribution to explanining the variability in family system outcomes (family system coping ). The subject for this study was 46 families who have a child with developmental disabilities(mental retardation and / or autism) from three special educational programs in Seoul, Korea. Results from correlation and hierachial regression analysis revealed that perceived social support operated as a resiliency factor between family stress and coping. Child and family characteristics appeared to be important predictors of perceived social support and coping. In summary, there is evidence that the resource of social support as a family strength and capability was found to improve the family coping. These findings also must be viewed within the context that sample of families of children with disabilities was relatively small and eligible families from support group of special educational program.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.507-513
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• 2000

A bacteriocin-producing organism, Enterococcus sp. T7, was isolated from human fecal samples. Bacteriocin T7, named tentatively as the bacteriocin, was produced by Enterococcus sp. T7 and it inhibited some strains of Lactobacillus. Staphylococcus, Enterococcus, and Streptococcus, but not all the lactococci and gram-negative bacteria tested. Bacteriocin T7 inhibited the growth of Listeria monocytogenes Scott A, but the degree of inhibition was less than those for other sensitive gram-positive vacteria. Bacteriocin T7 in MRS broth started to produce at the middle of the exponential growth phase and the inhibitory activity reached its maximum level during the stationary growth phase. Bacteriocin T7 was stable against heat treatments, pH variations (pH 2-10), and exposure to organic solvents. The molecular weight of bacteriocin T7 was estimated to be 6.500 Da by SDS-PAGe. All these facts, including physico-chemical stabilities, small molecular size, and inhibition of Kisteria monocytogenes, indicate that bacteriocin T7 is likely to be a member of the class IIa bacteriocins.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.585-595
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• 2019

Analysis of bacterial communities based on their 16S rDNA sequences revealed the predominance of Proteobacteria (Aeromonas sp., Acinetobacter sp. and Thaueraamino aromatica sp.) and uncultured bacterium in activated sludge from the effluent treatment plant (ETP) of Mother Dairy, Calcutta (India). Each isolate was used for bioremediation of dairy wastewater with simultaneous conversion of nitrogenous pollutants into ammonia. A consortium developed using seven of these isolates and three Bacillus strains from different environmental origins could reduce 93% nitrate with simultaneous production of ammonia (626 ㎍/100 ml) within 20 h in non-aerated, immobilized conditions as compared to 82% nitrate reduction producing 2.4 ㎍/100 ml ammonia in 96 h with extensive aeration in a conventional ETP. The treated ammonia-rich effluent could be used instead of freshwater and fertilizer during cultivation of mung bean with 1.6-fold increase in grain yield. The ETP with the surrounding agricultural land makes this process a zero liquid discharge technology for using the biofertilizer generated. In addition, the process requires minimal energy supporting sustained environmental health. This method is thus proposed as an alternative approach for small-scale dairy ETPs.

• Advances in materials Research
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• 제5권4호
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• pp.299-318
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• 2016

Analytical investigations were performed of a longitudinal crack representing a cylindrical surface in circular shafts loaded in torsion with taking into account the non-linear material behavior. Both functionally graded and multilayered shafts were analyzed. It was assumed that the material is functionally graded in radial direction. The mechanical behavior of shafts was modeled by using non-linear constitutive relations between the shear stresses and shear strains. The fracture was studied in terms of the strain energy release rate. Within the framework of small strain approach, the strain energy release rate was derived in a function of the torsion moments in the cross-sections ahead and behind the crack front. The analytical approach developed was applied to study the fracture in a clamped circular shaft. In order to verify the solution derived, the strain energy release rate was determined also by considering the shaft complimentary strain energy. The effects were evaluated of material properties, crack location and material non-linearity on the fracture behavior. The results obtained can be applied for optimization of the shafts structure with respect to the fracture performance. It was shown that the approach developed in the present paper is very useful for studying the longitudinal fracture in circular shafts in torsion with considering the material non-linearity.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.403-407
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• 2007

Several marine. bacterial strains, which were isolated from seawater off the island Dokdo, Korea, were screened to find new bioactive compounds such as antibiotics. Among them, Donghaeana dokdonensis strain DSW-6 was found to produce antibacterial agents, and the agents were then purified and analyzed by LC-MS/MS and 1D- and 2D-NMR spectrometries. The bioactive compounds were successfully identified as cholic acid and glycine-conjugated glycocholic acid, the $7{\alpha}$-dehydroxylated derivatives (deoxycholic acid and glycodeoxycholic acid) of which were also detected in relatively small amounts. Other marine isolates, taxonomically different from DSW-6, were also able to produce the compounds in a quite different production ratio from DSW-6. As far as we are aware of, these bile acids are produced by specific members of the genus Streptomyces and Myroides, and thought to be general secondary metabolites produced by a variety of bacterial taxa that are widely distributed in the sea.

• Smart Structures and Systems
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• 제21권1호
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• pp.15-25
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• 2018

This work presents the buckling investigation of embedded orthotropic nanoplates such as graphene by employing a new refined plate theory and nonlocal small-scale effects. The elastic foundation is modeled as two-parameter Pasternak foundation. The proposed two-variable refined plate theory takes account of transverse shear influences and parabolic variation of the transverse shear strains within the thickness of the plate by introducing undetermined integral terms, hence it is unnecessary to use shear correction factors. Nonlocal governing equations for the single layered graphene sheet are obtained from the principle of virtual displacements. The proposed theory is compared with other plate theories. Analytical solutions for buckling loads are obtained for single-layered graphene sheets with isotropic and orthotropic properties. The results presented in this study may provide useful guidance for design of orthotropic graphene based nanodevices that make use of the buckling properties of orthotropic nanoplates.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.120-125
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• 1994

By using a synthetic medium simulated on the amino acid composition of soybeam, the effect of salt concentrations on the production of volatile organic acid by the strains of Zygosaccharomyces rouxii So-3101, a soy sauce yeast, was studied at the concentrations of 12.5, 18.0, 22.0 and 28.5% NaCl. The growth, consumption of glucose, and production of alcohol, total acid and volatile organic acid, showed the highest values at a concentration of 12.5% NaCl, and those values were decreased with an increase in the salt concentration. The ratio of volatile organic acid to total organic acid was remained at approximately the same level within the range of salt concentrations between 12.5~22.0%, whereas the ratio was decreased at a salt concentration of 28.5%. After incubation for 16 days, 8 volatile organic acids, i.e. acetic, propionic, n-butyric, isobutyric, isovaleric, isocaproic, n-caproic, and heptanoic acids, were detected by gas chromatography. Among the volatile organic acids, acetic acid was produced in the appreciable amiunt and its ratio to the other volatile acids was increased with an increase in the salt concentration.A small amount of isocaproic, propionic, isobutyric and isovaleric acids were produced, and n-caproic, n-butyric and heptanoic acids were detected only at the lower salt concentration.

• Computers and Concrete
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• 제6권2호
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• pp.167-185
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• 2009

Reinforced concrete floors constructed between movement restraints often crack seriously due to shrinkage after completion. One common mitigation measure is to construct the concrete floors in stages to allow part of the shrinkage movement to take place before completion. However, shrinkage movement analysis of concrete floors constructed in stages is quite cumbersome, as the structural configuration changes during construction, thus necessitating reanalysis of the partially completed structure at each stage. Herein, a finite element method for shrinkage movement analysis of concrete floors constructed in stages is developed. It analyses the whole structure, including the completed and uncompleted portions, at all stages. The same mesh is used all the time and therefore re-meshing and location matching are no longer necessary. This is achieved by giving negligibly small stiffness to the uncompleted portions, which in reality do not exist yet. In the analysis, the locked-in strains due to increase in elastic modulus as the concrete hardens and the creep of the hardened concrete are taken into account. Most important of all, this method would enable fully automatic shrinkage movement analysis for the purpose of construction control.