• Title/Summary/Keyword: Small Samples

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In situ analysis of the bacterial community associated with the Korean salty fermented seafood jeotgal

  • Hyunjun Kim;Yoomin Ahn;Chulhee Park;Eungbin Kim
    • Korean Journal of Environmental Biology
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    • v.39 no.4
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    • pp.515-522
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    • 2021
  • Jeotgal is a salty and fermented traditional Korean fish sauce. Unlike most other previous studies that investigated samples purchased from retail markets, this study focused on samples of jeotgal with traceable history to Yeonggwang, a timehonored fishing village in Korea. Three jeotgal samples, which were made from small yellow croakers, largehead hairtail, and miscellaneous fish, were selected based on information obtained from interviews with local craftsmen and literature reviews. Bacterial community profiles of the three jeotgal samples were investigated to identify indicator (and potentially core) bacteria for jeotgal ripening. The 16S rRNA gene-based metagenomic analysis revealed that the dominant phyla and classes, (Gammaproteobacteria, Betaproteobacteria, Bacilli, and Clostridia) of the three different jeotgal were identical, albeit with different composition ratios. Diversification was evident beginning at the order level. Interestingly, each dominant order was mainly comprised of single members even at the genus level. The dominant genera included Halomonas, Tetragenococcus, Halanaerobium, Pseudomonas, Massilia, and Lentibacillus. This observed genus-level heterogeneity suggests that there are diverse bacterial signatures in jeotgal and that these can be used as indicators for jeotgal ripening and/or as starters to increase its sensory quality and functionality.

Tailings fluidization under cyclic triaxial loading - a laboratory study

  • Do, Tan Manh;Laue, Jan;Mattson, Hans;Jia, Qi
    • Geomechanics and Engineering
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    • v.29 no.5
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    • pp.497-508
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    • 2022
  • Tailings fluidization (i.e., tailings behave as being fluidized) under cyclic loading is one concern during the construction of tailings dams, especially in the shallow tailings layers. The primary goal of this study is to evaluate the responses of tailings under cyclic loadings and the tailings potential for fluidization. A series of cyclic triaxial undrained and drained tests were performed on medium and dense tailings samples under various cyclic stress ratios (CSR). The results indicated that axial strain and excess pore water pressure accumulated over time due to cyclic loading. However, the accumulations were dependent on CSR values, densities, and drainage conditions. The fluidization potential analysis in this study was then evaluated based on the obtained cyclic axial strain and excess pore water pressure. As a result, tailings samples were stable (unfluidized) under small CSR values, and the critical CSR values, where the tailings fluidized, varied depending on the density of tailings samples. Tailings fluidization is triggered as cyclic stress ratios reach critical values. In this study, the critical CSR values were found to be 0.15 and 0.40 for medium and dense samples, respectively.

Load of Soil Layers Established with Perlite (펄라이트로 조성된 토양층의 하중)

  • 이성기;류남형;허근영
    • Journal of the Korean Institute of Landscape Architecture
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    • v.30 no.1
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    • pp.87-95
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    • 2002
  • This study was carried out to investigate the load of soil layers affected by soil depth in artificial soil alone or in blends with Loam with various ratio. The artificial soils were perlite large grain, perlite small grain, and perlite small grains blended with Loam (sand 46%, silt 40%, clay 14%) at a ratio of 8:2, 6:4, 5:5 (v/v). The soil layers were divided into a planting layer and a well-drained layer, then the weight of each layer in the air-dried state and in the field capacity were determined. The data were subjected to correlation analysis, regression analysis, and paired samples t-test. The summarized results are as follows; 1) In the air-dried state, the regression equations of the well-drained layer weight(kg/m2) in perlite large grain, planting layer weight in perlite small grain, planting layer weight in perlite small grain biended with loam(8:2, v/v), perlite small grain blended with loam(6:4, v/v), and perlite small grain blended with loam(5:5, v/v) were; 1.65824*X+0.026, 1.52292*X-0.052, 3.21468*X+0.515, 6.17549*X+ 0.083, and 6.02100*X + 33.133, respectively, where X is soil depth measured in Centimeters. 2) In the field capacity, the regression equations of the well-drained layer weight(kg/m2) in perlite large grain, planting layer weight in perlite small grain, planting layer weight in perlite small grain blended with loam(8:2, v/v), perlite small grain blended with loam(6:4, v/v), and perlite small grain blended with loam(5:5, v/v) were 5.055*X - 2.006, 7.073*X + 100.008, 8.092*X + 116.676, 10.766*X + 100.112, and 10.974*X + 124.423, respectively, where X is the soil depth measured in Centimeters. 3) All of the equations mentioned above were statistically reliable and therefore easily applicable in practical business affairs.

Comparative analysis on genome-wide DNA methylation in longissimus dorsi muscle between Small Tailed Han and Dorper×Small Tailed Han crossbred sheep

  • Cao, Yang;Jin, Hai-Guo;Ma, Hui-Hai;Zhao, Zhi-Hui
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.11
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    • pp.1529-1539
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    • 2017
  • Objective: The objective of this study was to compare the DNA methylation profile in the longissimus dorsi muscle between Small Tailed Han and Dorper${\times}$Small Tailed Han crossbred sheep which were known to exhibit significant difference in meat-production. Methods: Six samples (three in each group) were subjected to the methylated DNA immunoprecipitation sequencing (MeDIP-seq) and subsequent bioinformatics analyses to detect differentially methylated regions (DMRs) between the two groups. Results: 23.08 Gb clean data from six samples were generated and 808 DMRs were identified in gene body or their neighboring up/downstream regions. Compared with Small Tailed Han sheep, we observed a tendency toward a global loss of DNA methylation in these DMRs in the crossbred group. Gene ontology enrichment analysis found several gene sets which were hypomethylated in gene-body region, including nucleoside binding, motor activity, phospholipid binding and cell junction. Numerous genes were found to be differentially methylated between the two groups with several genes significantly differentially methylated, including transforming growth factor beta 3 (TGFB3), acyl-CoA synthetase long chain family member 1 (ACSL1), ryanodine receptor 1 (RYR1), acyl-CoA oxidase 2 (ACOX2), peroxisome proliferator activated receptor-gamma2 (PPARG2), netrin 1 (NTN1), ras and rab interactor 2 (RIN2), microtubule associated protein RP/EB family member 1 (MAPRE1), ADAM metallopeptidase with thrombospondin type 1 motif 2 (ADAMTS2), myomesin 1 (MYOM1), zinc finger, DHHC type containing 13 (ZDHHC13), and SH3 and PX domains 2B (SH3PXD2B). The real-time quantitative polymerase chain reaction validation showed that the 12 genes are differentially expressed between the two groups. Conclusion: In the current study, a tendency to a global loss of DNA methylation in these DMRs in the crossbred group was found. Twelve genes, TGFB3, ACSL1, RYR1, ACOX2, PPARG2, NTN1, RIN2, MAPRE1, ADAMTS2, MYOM1, ZDHHC13, and SH3PXD2B, were found to be differentially methylated between the two groups by gene ontology enrichment analysis. There are differences in the expression of 12 genes, of which ACSL1, RIN2, and ADAMTS2 have a negative correlation with methylation levels and the data suggest that DNA methylation levels in DMRs of the 3 genes may have an influence on the expression. These results will serve as a valuable resource for DNA methylation investigations on screening candidate genes which might be related to meat production in sheep.

Analysis of Spatial Variability in a Korean Paddy Field Using Median Polish Detrending (Median polish 기법을 이용한 한국 논의 공간변이 분석)

  • Chung, Sun-Ok;Jung, In-Kyu;Sung, Je-Hoon;Sudduth, Kenneth A.;Drummond, Scott T.
    • Journal of Biosystems Engineering
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    • v.33 no.5
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    • pp.362-369
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    • 2008
  • There is developing interest in precision agriculture in Korea, despite the fact that typical Korean fields are less than 1 ha in size. Describing within-field variability in typical Korean production settings is a fundamental first step toward determining the size of management zones and the inter-relationships between limiting factors, for establishment of site-specific management strategies. Measurements of rice (Oriza Sativa L) yield, chlorophyll content, and soil properties were obtained in a small (100-m by 30-m) Korean rice paddy field. Yield data were manually collected on 10-m by 5-m grids (180 samples with 3 samples in each of 60 grid cells) and chlorophyll content was measured using a Minolta SPAD 502 in 2-m by 2-m grids. Soil samples were collected at 275 points to compare results from sampling at different scales. Ten soil properties important for rice production in Korea were determined through laboratory analyses. Variogram analysis and point kriging with and without median polishing were conducted to determine the variability of the measured parameters. Influence of variogram model selection and other parameters on the interpretation of the data was investigated. For many of the data, maximum values were greater than double the minimum values, indicating considerable spatial variability in the small paddy field, and large-scale spatial trends were present. When variograms were fit to the original data, the limits of spatial dependency for rice yield and SP AD reading were 11.5 m and 6.5 m, respectively, and after detrending the limits were reduced to 7.4 m and 3.9 m. The range of spatial dependency for soil properties was variable, with several having ranges as short as 2 m and others having ranges greater than 30 m. Kriged maps of the variables clearly showed the presence of both large-scale (trend) variability and small-scale variability in this small field where it would be reasonable to expect uniformity. These findings indicate the potential for applying the principles and technology of precision agriculture for Korean paddy fields. Additional research is needed to confirm the results with data from other fields and crops.d similar tendency with the result for the frequency less than 20 Hz, but the width of change was reduced highly.

Characteristics of Nonpoint Source Pollutant Loads from Forest watershed with Various Water Quality Sampling Frequencies (수질샘플빈도에 따른 산림유역의 비점원오염부하특성)

  • Shin, Min-Hwan;Shi, Yong-Chul;Heo, Sung-Gu;Lim, Kyoung-Jae;Choi, Joong-Dae
    • Journal of The Korean Society of Agricultural Engineers
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    • v.50 no.2
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    • pp.65-71
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    • 2008
  • A monsoon season monitoring data from June to September, 2005 of a small forested watershed located at the upstream of the North Han River system in Korea was conducted to analyze the flow variations, the NPS pollutant concentrations, and the pollution load characteristics with respect to sampling frequencies. During the 4-month period, 1,423 mm or 79.2% of annual rainfall(1,797 mm) were occurred and more than 77%, 54% and 68% of annual T-N, $NO_3$-N and T-P loads discharged. Flow rate was continuously measured with automatic velocity and water level meters and 58 water quality samples were taken and analyzed. It was analyzed that the flow volume by random measurement varied very widely and ranged from 79% to 218% of that of continuous measurement. It was recommended that flow measurement of small forested watersheds should be continuously measured with automated flow meters to precisely measure flow rates. Flow-weighted mean concentrations of T-N, $NO_3$-N and T-P during the period were 2.114 mg/L, 0.836 mg/L, and 0.136 mg/L, respectively. T-N, $NO_3$-N and T-P loads were sensitive to the number of samples. And it was analyzed that in order to measure the pollution load within the error of 10% to the true load, the rate of sampling frequency should be higher than 89.7% of the sample numbers that were required to compute the true pollution load. If it is compared to selected foreign research results, about 10 water samples for each rainfall event were needed to compute the pollution load within 10% error. It is unlikely in Korea and recommended that thorough NPS pollution monitoring studies are required to develop the standard monitoring procedures for reliable NPS pollution quantification.

Ultrasound Probe Contamination Classification using ATP Meter (ATP meter를 이용한 초음파 탐촉자의 오염도 분류)

  • Ha, Myeong-Jin;Kim, Jeong-Koo
    • Journal of radiological science and technology
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    • v.43 no.1
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    • pp.1-7
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    • 2020
  • In this study, the contamination level was measured using an ATP meter using adenosine triphosphate bioluminescent material to find effective infection control to compensate for the disadvantages of the microbial culture method used for hygiene control of ultrasound probe. The convex probes were selected from six ultrasound probe in the hospital, and the samples were taken in real time before and after cleaning to check the contamination of the probe. In order to classify the pollution degree using the APT meter was classified by category. A total of 78 samples were collected from the ultrasound probe. When the pollution levels before and after cleaning were classified by category, 76.6% of the samples were classified into category 3·4 before cleaning, but they decreased to 23.3% after cleaning. 13.3% before cleaning was in category 1, but increased to 43.3% after cleaning. By classifying the pollution level, it was confirmed that the pollution level was significantly reduced by category. Until now, there was no suitable criterion for determining the contamination level by using ATP meter in medical machines where sample area is small and reused. In this study, criteria for each category were set to measure the contamination level of ATP meter suitable for small sample area such as ultrasound probe, so that contamination level could be determined in real time at the site. Therefore, it is considered that hygiene management for ultrasound probe can be more actively performed.

Research on the Actual Condition of Shape and Make-up of Peanut-Shaped Cloth Diaper for Baby on Market (시판중인 유아용 땅콩기저귀의 형태와 구성에 관한 실태조사)

  • Lee, Jung-Soon;Han, Gyung-Hee;Koo, Mi-Ran
    • Journal of Fashion Business
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    • v.15 no.4
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    • pp.122-134
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    • 2011
  • The peanut-shaped cloth diaper has cut uncomfortable crotch region off the square-shaped cloth diaper into a peanut-shape, and finished the edge by bias with multi-layers of fabric. This study has the purpose on providing the basic data for designing the peanut-shaped cloth diaper. For this purpose, the peanut-shaped cloth diapers on market were collected and analyzed by their shape and size, and also the patterns and differences in making were compared. 26 products from 17 brands were collected as samples. The analysis factors are the shape and size of the peanut-shaped cloth diaper, and characteristics and patterns in making. Among the samples, only 3 diapers were straight-shaped; the rest of them were peanut-shaped. The size difference between the big and small peanut-shaped cloth diapers was very large; the total length of the small one was 36.5cm-39cm which was very common, and the total length of the big one was more than 44cm. All diapers had longer width in the back than the front. The width of the front and back was different for each sample, therefore, it seemed to have some difficulties to set the standard. The peanut-shaped cloth diaper had different make-up depending on its characteristics including. absorbance, washing and drying. It was usually made by cotton woven of diamond jacquard or knit, and finished the edge by woven bias or knit bias. The peanut-shaped cloth diapers were various including a sheet of diaper, two sheets of diapers fixed with velcro, three sheets of diapers, and etc. For the patterns of the diapers, only two samples out of all had three dimensional shape, and the rest were produced using flat shape. There were no distinction between the diapers of girls and boys. Therefore, it is necessary to make the product based on scientific data about the peanut-shaped cloth diaper.

Redesigning KNSO s Household Survey Sample (통계청 가구부문 조사의 표본설계)

  • 윤연옥;김규영;이명호
    • Survey Research
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    • v.5 no.1
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    • pp.103-130
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    • 2004
  • Main monthly household surveys conducted by Korea National Statistical Office are economically active population survey(EAPS) and household income and expenditure survey(HIES). Samples of these two surveys are redesigned every 5 years based on Census. This paper is about sample redesign of household survey conducted in 2002 based on 2000 Census. Main improvements of 2002 sample redesign are the introduction of rotation sampling system, the expansion of HIES survey area from urban to whole country and the foundation of basement to make small area estimation for the unemployment statistics. Also the number of sample households within a enumeration district(ED) is reduced from 24 to 20. That makes it possible to select more ED samples which provides better precision for EAPS and HIES. To select representative samples for the population, different classification index is used for each metropolitan area and provinces.

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Cracks evolution and multifractal of acoustic emission energy during coal loading

  • Kong, Xiangguo;Wang, Enyuan;He, Xueqiu;Liu, Xiaofei;Li, Dexing;Liu, Quanlin
    • Geomechanics and Engineering
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    • v.14 no.2
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    • pp.107-113
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    • 2018
  • Coal samples with different joints morphology were subjected to uniaxial compression experiments, cracks evolution was recorded by Nikon D5300 and acoustic emission (AE) energy signals were collected by AEwin Test for Express-8.0. During loading process, coal samples deformed elastically with no obvious cracks changes, then they expanded gradually along the trace of the original cracks, accompanied by the formation of secondary cracks, and eventually produced a large-scale fracture. It was more interesting that the failure mode of samples were all shear shape, whatever the original cracks morphology was. With cracks and damage evolution, AE energy radiated regularly. At the early loading stage, micro damage and small scale fracture events only induced a few AE events with less energy, while large scale fracture leaded to a number of AE events with more energy at the later stage. Based on the multifractal theory, the multifractal spectrum could explain AE energy signals frequency responses and the causes of AE events with load. Multifractal spectrum width (${\Delta}{\alpha}$), could reflect the differences between the large and small AE energy signals. And another parameter (${\Delta}f$) could reflect the relationship between the frequency of the least and greatest signals in the AE energy time series. This research is helpful for us to understand cracks evolution and AE energy signals causes.