Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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In situ analysis of the bacterial community associated with the Korean salty fermented seafood jeotgal

  • Hyunjun Kim (Department of Systems Biology, Yonsei University) ;
  • Yoomin Ahn (Department of Systems Biology, Yonsei University) ;
  • Chulhee Park (Department of Systems Biology, Yonsei University) ;
  • Eungbin Kim (Department of Systems Biology, Yonsei University)
  • Received : 2021.05.24
  • Accepted : 2021.12.07
  • Published : 2021.12.31
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Abstract

Jeotgal is a salty and fermented traditional Korean fish sauce. Unlike most other previous studies that investigated samples purchased from retail markets, this study focused on samples of jeotgal with traceable history to Yeonggwang, a timehonored fishing village in Korea. Three jeotgal samples, which were made from small yellow croakers, largehead hairtail, and miscellaneous fish, were selected based on information obtained from interviews with local craftsmen and literature reviews. Bacterial community profiles of the three jeotgal samples were investigated to identify indicator (and potentially core) bacteria for jeotgal ripening. The 16S rRNA gene-based metagenomic analysis revealed that the dominant phyla and classes, (Gammaproteobacteria, Betaproteobacteria, Bacilli, and Clostridia) of the three different jeotgal were identical, albeit with different composition ratios. Diversification was evident beginning at the order level. Interestingly, each dominant order was mainly comprised of single members even at the genus level. The dominant genera included Halomonas, Tetragenococcus, Halanaerobium, Pseudomonas, Massilia, and Lentibacillus. This observed genus-level heterogeneity suggests that there are diverse bacterial signatures in jeotgal and that these can be used as indicators for jeotgal ripening and/or as starters to increase its sensory quality and functionality.

Keywords

Acknowledgement

This work was supported by the Strategic Initiative for Microbiomes in Agriculture and Food (918011-4) funded by the Ministry of Agriculture, Food and Rural Affairs and by the Ministry of Education of the Republic of Korea and the National Research Foundation of Korea (NRF-2019S1A5C2A04083293).

References

  1. Cha IT, MH Lee, BY Kim, YJ Cho, DW Kim, KJ Yim, HS Song, MJ Seo, JK Rhee, JS Choi, HJ Choi, C Yoon, SW Roh and YD Nam. 2015. Genome sequence of the haloarchaeon Haloterrigena jeotgali type strain A29 (T) isolated from salt-fermented food. Stand. Genomic Sci. 10:49.
  2. Chao A. 1987. Estimating the population size for capture-recapture data with unequal catchability. Biometrics 43:783-791. https://doi.org/10.2307/2531532
  3. Choi EJ, HM Jin, KH Kim and CO Jeon. 2014. Salimicrobium jeotgali sp. nov., isolated from salted, fermented seafood. Int. J. Syst. Evol. Microbiol. 64:3624-3630. https://doi.org/10.1099/ijs.0.062042-0
  4. Choi EJ, SH Lee, JY Jung and CO Jeon. 2013. Brevibacterium jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 63:3430-3436. https://doi.org/10.1099/ijs.0.049197-0
  5. Guan L, KH Cho and JH Lee. 2011. Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria. Food Microbiol. 28:101-113.
  6. Huh SH. 1999. Feeding habits of hairtail, Trichiurus lepturus. Korean J. Ichthyol. 11:191-197.
  7. Hyun DW, MJ Jung, MS Kim, NR Shin, PS Kim, TW Whon and JW Bae. 2016. Proteus cibarius sp. nov., a swarming bacterium from jeotgal, a traditional Korean fermented seafood, and emended description of the genus Proteus. Int. J. Syst. Evol. Microbiol. 66:2158-2164. https://doi.org/10.1099/ijsem.0.001002
  8. Jeong DW, SH Han and JH Lee. 2014. Safety and technological characterization of Staphylococcus equorum isolates from jeotgal, a Korean high-salt-fermented seafood, for starter development. Int. J. Food Microbiol. 188:108-115. https://doi.org/10.1016/j.ijfoodmicro.2014.07.022
  9. Jeong DW, HR Kim and JH Lee. 2014. Genetic diversity of Staphylococcus equorum isolates from saeu-jeotgal evaluated by multilocus sequence typing. Antonie Van Leeuwenhoek 106:795-808. https://doi.org/10.1007/s10482-014-0249-6
  10. Jeong DW, HJ Na, SR Ryu and JH Lee. 2016. Complete genome sequence of Staphylococcus equorum KS1039 isolated from saeu-jeotgal, Korean high-salt-fermented seafood. J. Biotechnol. 219:88-89. https://doi.org/10.1016/j.jbiotec.2015.12.025
  11. Jeong SH, JH Lee, JY Jung, SH Lee, MS Park and CO Jeon. 2013. Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Antonie van Leeuwenhoek 103:503-512. https://doi.org/10.1007/s10482-012-9832-x
  12. Jung DW, GS Jung and JH Lee. 2016. Cultivable bacterial community analysis of saeu-jeotgal, a Korean high-salt-fermented seafood, during ripening. Microbiol. Biotechnol. Lett. 44:293-302. https://doi.org/10.4014/mbl.1606.06007
  13. Jung J, S Choi, CO Jeon and W Park. 2013. Pyrosequencing-based analysis of the bacterial community in Korean traditional seafood, ojingeo jeotgal. J. Microbiol. Biotechnol. 23:1428-1433. https://doi.org/10.4014/jmb.1305.05018
  14. Jung MY, TW Kim, C Lee, JY Kim, HS Song, YB Kim, SW Ahn, JS Kim, SW Roh and SH Lee. 2018. Role of jeotgal, a Korean traditional fermented fish sauce, in microbial dynamics and metabolite profiles during kimchi fermentation. Food Chem. 265:135-143. https://doi.org/10.1016/j.foodchem.2018.05.093
  15. Kim JA, Z Yao, HJ Kim and JH Kim. 2019. Physicochemical properties and bacterial communities of meongge (Halocynthia roretzi) jeotgal prepared with 3 different types of salts. J. Microbiol. Biotechnol. 29:527-537. https://doi.org/10.4014/jmb.1901.01016
  16. Kim JA, Z Yao, V Perumal and HJ Kim. 2018. Properties of Tetragenococcus halophilus strains isolated from myeolchi (anchovy)-jeotgal. Microbiol. Biotechnol. Lett. 46:313-319. https://doi.org/10.4014/mbl.1804.04016
  17. Kim KH, SH Lee, BH Chun, SE Jeong and CO Jeon. 2019. Tetragenococcus halophilus MJ4 as a starter culture for repressing biogenic amine (cadaverine) formation during saeu-jeot (salted shrimp) fermentation. Food Microbiol. 82:465-473. https://doi.org/10.1016/j.fm.2019.02.017
  18. Kim MS and EJ Park. 2014. Bacterial communities of traditional salted and fermented sea foods from Jeju Island of Korea using 16s rRNA gene clone library analysis. J. Food Sci. 79:927-934.
  19. Kim MS, SW Roh and JW Bae. 2010. Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. J. Microbiol. 48:404-410. https://doi.org/10.1007/s12275-010-0032-y
  20. Koo OK, SJ Lee, KR Chung, DJ Jang, HJ Yang and DY Kwon. 2016. Korea traditional fermented fish products: jeotgal. J. Ethn. Foods 3:107-116. https://doi.org/10.1016/j.jef.2016.06.004
  21. Lee JH, SJ Heo, MR Jeong and DW Jeong. 2019. Transfer of a mobile Staphylococcus saprophyticus plasmid isolated from fermented seafood that confers tetracycline resistance. PLoS One 14:e0213289.
  22. Lee JM, YR Kim, JH Kim, GT Jeong, JC Ha and IS Kong. 2015. Characterization of salt-tolerant β -glucosidase with increased thermostability under high salinity conditions from Bacillus sp. SJ-10 isolated from jeotgal, a traditional Korean fermented seafood. Bioproc. Biosyst. Eng. 38:1335-1346. https://doi.org/10.1007/s00449-015-1375-x
  23. Lee SH, JY Jung and CO Jeon. 2014. Microbial successions and metabolite changes during fermentation of salted shrimp (saeu-jeot) with different salt concentrations. PLoS One 9:e90115.
  24. Lee SH, HJ Ku, MJ Ahn, JS Hong, SH Lee, HD Shin, KC Lee, JS Lee, SR Ryu, CO Jeon and JH Lee. 2015. Weissella jogaejeotgali sp. nov., isolated from jogae jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 65:4674-4681. https://doi.org/10.1099/ijsem.0.000631
  25. Lee Y, Y Cho, E Kim, HJ Kim and HY Kim. 2018. Identification of lactic acid bacteria in galchi- and myeolchi-jeotgal by 16s rRNA gene sequencing, MALDI-TOF mass spectrometry, and PCR-DGGE. J. Microbiol. Biotechnol. 28:1112-1121. https://doi.org/10.4014/jmb.1803.03034
  26. Magurran AE. 2003. Measuring Biological Diversity. Wiley-Blackwell. Hoboken, NJ.
  27. Mah JH, YH Chang and HJ Hwang. 2008. Paenibacillus tyraminigenes sp. nov. isolated from myeolchi-jeotgal, a traditional Korean salted and fermented anchovy. Int. J. Food Microbiol. 127:209-214. https://doi.org/10.1016/j.ijfoodmicro.2008.07.002
  28. Nam YD, MJ Seo, SI Lim and SL Park. 2012. Genome sequence of Kocuria atrinae C3-8, isolated from jeotgal, a traditional Korean fermented seafood. J. Bacteriol. 194:5996.
  29. Roh SW, KH Kim, YD Nam, HW Chang, EJ Park and JW Bae. 2010. Investigation of archaeal and bacterial diversity in fermented seafood using barcoded pyrosequencing. ISME J. 4:1-16. https://doi.org/10.1038/ismej.2009.83
  30. Roh SW, YD Nam, HW Chang, YB Sung, KH Kim, HM Oh and JW Bae. 2007. Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57:2296-2298. https://doi.org/10.1099/ijs.0.65121-0
  31. Roh SW, YD Nam, HW Chang, Y Sung, KH Kim, HJ Lee, HM Oh and JW Bae. 2007. Natronococcus jeotgali sp. nov., a halophilic archaeon isolated from shrimp jeotgal, a traditional fermented seafood from Korea. Int. J. Syst. Evol. Microbiol. 57:2129-2131. https://doi.org/10.1099/ijs.0.65120-0
  32. Shim JM, KW Lee, Z Yao, JA Kim, HJ Kim and JH Kim. 2017. Microbial communities and physicochemical properties of myeolchi jeotgal (anchovy jeotgal) prepared with different types of salts. J. Microbiol. Biotechnol. 27:1744-1752. https://doi.org/10.4014/jmb.1702.02027
  33. Song EJ, ES Lee, SL Park, HJ Choi, SW Roh and YD Nam. 2018. Bacterial community analysis in three types of the fermented seafood, jeotgal, produced in South Korea. Biosci. Biotechnol. Biochem. 82:1444-1454. https://doi.org/10.1080/09168451.2018.1469395
  34. Sundararaman A, S Srinivasan, JH Lee and SS Lee. 2017. Virgibacillus jeotgali sp. nov., isolated from myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy. Int. J. Syst. Evol. Microbiol. 67:158-163. https://doi.org/10.1099/ijsem.0.001596
  35. Sundararaman A, S Srinivasan, JH Lee and SS Lee. 2018. Lentibacillus alimentarius sp. nov., isolated from myeolchi-jeotgal, a traditional Korean high-salt fermented anchovy. Antonie van Leeuwenhoek 111:1065-1071. https://doi.org/10.1007/s10482-017-1006-4
  36. Sung JS, J Chun, S Choi and W Park. 2012. Genome sequence of the halotolerant Staphylococcus sp. strain OJ82, isolated from Korean traditional salt-fermented seafood. J. Bacteriol. 194:6353-6354. https://doi.org/10.1128/JB.01653-12
  37. Udomsil N, S Rodtong, YJ Choi, Y Hua and J Yongsawatdigul. 2011. Use of Tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation. J. Agr. Food Chem. 59:8401-8408. https://doi.org/10.1021/jf201953v
  38. Whon TW, DW Hyun, YD Nam, MS Kim, EJ Song, YK Jang, ES Jung, NR Shin, SJ Oh, PS Kim, HS Kim, CH Lee and JW Bae. 2015. Genomic and phenotypic analyses of Carnobacterium jeotgali strain MS3 (T), a lactate-producing candidate biopreservative bacterium isolated from salt-fermented shrimp. FEMS Microbiol. Lett. 362:fnv058.
  39. Yao Z, JA Kim and JH Kim. 2018. Properties of a fibrinolytic enzyme secreted by Bacillus subtilis JS2 isolated from saeu (small shrimp) jeotgal. Food Sci. Biotechnol. 27:765-772. https://doi.org/10.1007/s10068-017-0299-4
  40. Yao Z, JA Kim and JH Kim. 2019. Characterization of a fibrinolytic enzyme secreted by Bacillus velezensis BS2 isolated from sea squirt jeotgal. J. Microbiol. Biotechnol. 29:347-356. https://doi.org/10.4014/jmb.1810.10053
  41. Yoon JH, SH Yeo, TK Oh and YH Park. 2005. Psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 55:171-176. https://doi.org/10.1099/ijs.0.63140-0
  42. Yuk KJ, YT Kim, CS Huh and JH Lee. 2018. Lelliottia jeotgali sp. nov., isolated from a traditional Korean fermented clam. Int. J. Syst. Evol. Microbiol. 68:1725-1731. https://doi.org/10.1099/ijsem.0.002737
  43. Yun JH, SW Roh, MS Kim, MJ Jung, EJ Park, KS Shin, YD Nam and JW Bae. 2011. Leucobacter salsicius sp. nov., from a salt-fermented food. Int. J. Syst. Evol. Microbiol. 61:502-506. https://doi.org/10.1099/ijs.0.021360-0