• 태양에너지
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• 제9권3호
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• pp.81-91
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• 1989

This study represents the method to predict the flow duration curve and primary design specifications of small hydropower plant at hydropower site through analyzing the monthly rainfall data. Weibull distribution was selected to characterize the rainfall data and Thiessen method was used to calculate monthly average flowrate at site. Application of these results, primary design specifications such as design flowrate, annual average load factor and utility factor, annual average hydropower density and annual electric energy production were estimated and discussed for surveyed site located in Daigi-ri, Kangwon province. And performance characteristic model of small hydro-power plant was applied to estimate these specifications.

• 한국환경복원기술학회지
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• 제4권1호
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• pp.90-97
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• 2001

For improvement of water quality, $20m^2$ of artificial floating plant islands planted with Iris pseudoacorus, were installed in small pond on March, 1999. Small pond has surface area $1,000m^2$ and mean depth 1.5 m. The density of plants was 16 per $m^2$ by using jute pot. Environmental parameters such as COD, SS, T-N, T-P and planktons were biweekly measured from 29 March to 28 September. Because of the small portion of floating island, the effect for water quality improvement was not sufficient. But considering the data of plant growth and nitrogen and phosphorus uptake capacity of plant, about 40% of coverage by artificial floating island was needed for elimination of whole nutrients from inflow.

• The Plant Pathology Journal
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• 제38권5호
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• pp.533-540
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• 2022

Thunberg fritillary (Fritillaria thunbergii), a perennial used in traditional Chinese herbal medicine, is a members of the family Liliaceae. The degeneration of germplasm is a severe problem in the production of Fritillaria thunbergii var. chekiangensis. However, no information about viral infections of F. thunbergii var. chekiangensis has been reported. In this study, we sequenced the small RNAs of F. thunbergii var. chekiangensis from leaves and bulbs, and viruses were identified using a phylogenetic analysis and BLAST search for sequence. In addition, multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was used to rapidly detect viruses in this variety. Our study first reported that five viruses infected F. thunbergii var. chekiangensis. Among them, fritillary virus Y (FVY), lily mottle virus (LMoV), Thunberg fritillary mosaic virus (TFMV), and hop yellow virus (HYV) had been reported in F. thunbergii, while apple stem grooving virus was first reported in the genus Fritillaria. A multiplex RT-PCR method was developed to rapidly test the four viruses FVY, LMoV, TFMV, and HYV in F. thunbergii var. chekiangensis. Our results provide a better understanding of the infection of F. thunbergii var. chekiangensis by viruses and a basic reference for the better design of suitable control measures.

• The Plant Pathology Journal
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• 제22권3호
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• pp.235-238
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• 2006

Chrysanthemum stunt viroid (CSVd) induced systemic symptoms on chrysanthemum. We detected small RNAs of approximately 22 nucleotides with sequence specificity to CSVd in chrysanthemum infected with CSVd: an indication of the presence of RNA silencing. Regardless of symptom differences associated with CSVd, the small RNAs distributed similarly in amount. Small RNAs were detected with partial-length or full-length probes, indicating that they are not restricted to specific viroid regions but likely representing most of the viroid molecule.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.134-137
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• 2008

A methodology to evaluate the performance analysis for small hydropower at existing water treatment facilities has been studied. It consists of two main parts; flow duration function which can describe existing water treatment facilities and performance analysis to estimate the output characteristics of small hydro power plants. The output performance characteristics for Mi-ho reservoir, Sum-kang low dam, Sun-cheon sewage treatment plant and Nam-dong purification plant were analyzed, using developed model. According to the simulation results, the predicted data show that the data were in good agreement with measured results. Also, it was found that the developed model in this study can be used to analyze the output characteristics for small hydro power at existing water treatment facilities.

• 신재생에너지
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• 제2권2호
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• pp.81-85
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• 2006

The output performance characteristics for surveyed sites were analyzed, using developed model. It consists of two main parts, the deciding flow duration characteristic of river and performance prediction model to estimate the output characteristics of small hydropower plants. As a result, It was found that the flowrate concerning with 25% of time ratio on flow duration curve can be selected to design flowrate of small hydropower plants, and the output characteristics of small hydropower plants having overflow dam are different from large scale hydropower plants.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.491-494
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• 2006

The output performance characteristics for surveyed sites were analyzed, using developed model. It consists of two main parts, the deciding flow duration characteristic of river and performance prediction model to estimate the output characteristics of small hydropower plants. As a result, it was found that the flowrate concerning with 25% of time ratio on flow duration curve can be selected to design flowrate of small hydropower plants, and the output characteristics of small hydropower plants having overflow dam are different from large scale hydropower plants.

• The Plant Pathology Journal
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• 제20권2호
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• pp.142-146
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• 2004

Woody plant tissues contain great amounts of phenolic compounds and polysaccharides. These substances inhibit the activation of reverse transcriptase and/or Taq polymerase in RT-PCR. The commonly used multiple-step protocols using several additives to diminish polyphenolic compounds during nucleic acid extraction are time consuming and laborious. In this study, sodium sulfite was evaluated as an additive for nucleic acid extraction from woody plants and the efficiency of RT-PCR assay of commercial nucleic acid extraction kits and small-scale dsRNA extraction was compared. Sodium sulfite was used as an inhibitor against polyphenolic oxidases and its effects were compared in RNA extraction by commercial extraction kit and small-scale double-stranded RNA (dsRNA) extraction method for RT-PCR. During nucleic acid extraction, addition of 0.5%-1.5%(w/v) of sodium sulfite to lysis buffer or STE buffer resulted in lighter browning by oxidation than extracts without sodium sulfite and improved the RT-PCR detection. When commercial RNA extraction kit was used, optimal concentrations of sodium sulfite were variable according to the tested plant. However, with dsRNA as RT-PCR template, sodium sulfite 1.5% in STE buffer improved the detection efficiency of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in fruit trees, and reduced the unspecific amplifications signi-ficantly. Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

• 농촌계획
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• 제12권4호
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• pp.83-88
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• 2006

Recently, management and restoration efforts using the plants promote the disturbed habitats such as a set-asides or field boundaries. But, side effects are coming out because of using the exotic plants in restoration process. This study was conducted to select the native plant species used for restoration through the vegetation survey and plant community analysis at small stream-side in rural villages. We surveyed at two small stream-sides in open field and high land area in 2005 as a case study. Total 126 species was found at small stream side in open field area. As the result of important value' calculation, Persicaria thunbergii 23.0%, Humulus japonicus 18.6%, Phragmites communis 4.4%, Bromus japonicus 4.2%, and Rosa multiflora 3.4% were in the order of important value. Total 92 species were found at small stream side in high land area. As the result of important value' calculation, Dactylis glomerata 16.1%, Artemisia princeps var. orientalis 11.0%, Persicaria thunbergii 10.9%, Humulus japonicus 9.3%, Phragmites japonica 5.2% and Phragmites communis 3.5% were in the order of important value. We selected some plant species to use in restoration after due consideration of problems of exotic plant and high coverage and density species, and ecological process; Persicaria thunberii, Phragmites communis, Bromus japonicus, Rosa multiflora, Equisetum arvense, Digtaria sanguinalis, Impatiens textori, and Artemisia princeps var. orientalis in open field stream-side, and Artemisia princeps var. orientalis, Persicaria thunbergii, Phragmites japonica, Phragmites communis, Artemisia selengensis, Panicum bisulcatum, Rorippa indica, and Equisetum arvense at hghland stream-side. We will verify the selected native plants and plan the utilization of the native plants in restoration at stream-side in rural village.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1628-1634
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• 2009

Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17.7, cytosolic class I sHSPs, were cloned from a tropical plant, Ageratina adenophorum. Their C-terminal domains were highly conserved with those of sHSPs from other plants, indicating the importance of the C-terminal domains for the structure and activity of sHSPs. The recombinant HSP17.6 and HSP17.7 were applied to determine their chaperone function. In vitro, HSP17.6 and HSP17.7 actively participated in the refolding of the model substrate citrate synthase (CS) and effectively prevented the thermal aggregation of CS at $45^{\circ}C$ and the irreversible inactivation of CS at $38^{\circ}C$ at stoichiometric levels. The prior presence of HSP17.7 was assumed to suppress the thermal aggregation of the model substrate CS. Therefore, this report confirms the chaperone activity of HSP17.6 and HSP17.7 and their potential as a protectant for active proteins.