• 한국육종학회지
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• 제43권4호
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• pp.260-264
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• 2011

Leaf structure is one of the important agronomic traits. A rolled leaf mutant was induced from an ethyl methane sulfonate (EMS)-treated japonica rice, 'Koshihikari'. The rolled leaf mutant showed phenotypes of reduced leaf width and leaf rolling. In addition, several abnormal morphological characteristics were observed, including dwarfism, defected panicle, delayed germination, and lower seed-setting. Microscopic analysis revealed that the number of small veins was decreased and the sizes of adaxial bulliform cells were reduced in the mutant leaves. The genetic study with two $F_2$ populations from the crosses of the rolled leaf mutant with 'Koshihikari' and Milyang23 suggested that the mutant phenotype might be controlled by a single dominant gene.

• Journal of Plant Biotechnology
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• 제46권3호
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• pp.205-216
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• 2019

Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.

• 한국기술사회:학술대회논문집
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• 한국기술사회 1984년도 제14회 한일기술사 합동 심포지움 참관기
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• pp.77-82
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• 1984

The small group activities (SGA) was introduced into some of enterprises in Korea in 1967 and plant division, Saemaul undong Headquarters had encouraged Quality Control Circles (QCC) within the manufacturing corporations in assistance of central government. Registered small groups as of august 31, 1984 amounted up to 78,243 units and the number of members were 791,512. Meanwhile, small and medium industries have mostly introduced small group activities considerably later and less actively managed than large business. Main reasons for the less effectiveness of the activities are due to lack of management skills and less awareness of it from management and workers group. Effective small group activities are presumed to be successful only with labor management cooperation on the basis of human-oriented management philosophy. The small group activities are also prevalent in service sector. More derivative methods have been developed and more members are willingly participating in training programs. The small group which is basically a horizontal organization unit, promotes communication within the whole organization. In consideration of the social circumstances and traditions, the flexible model of the small group activities suitable to the corporate environment, will contribute to industrial development.

• 농업과학연구
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• 제16권2호
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• pp.138-151
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• 1989

In order to find the effects of seed size on the early seedling growth and yield of soybean, three soybean cultivars in Korea were investigated. Seed size was classified into large and small according to the weight and planted in pots(1/5000a) and in the field. Three soybean cultivars respresenting large, medium and small grains were Hwangkeum-kong, Kwangkyo and Bangsa-kong respectively. These cultivars were planted on June 20, 1987. 1. The plant height, stem diameter, root length and leaf area index(LAI) of the seed with large size seemed larger than the seed with small size regardless of cultivars. 2. The fresh and dry weight were different depending upon the grain sizes. The large grain had heavier fresh and dry weight than the small grains. 3. The protein consumption rate of the cotyledon of Bangsa-kong with small grain size was faster than the Hwangkeum-kong with large grain size. 4. The stem length, stem diameter and number of main stem node of the seed with large size seemed larger than the seed with small size. Large grains of Hwangkeum-kong were the highest in the number of branch node and number of node. 5. The number of pods and grains per plant of Bangsa-kong with small grain size was larger than the Kwangkyo with large grain size. 6. The yield per 10a for Hwangkeum-kong, Hwangkyo and Bangsa-kong were 226.3kg, 193.0kg and 192.8kg, respectively and they were all statistically different. The yield increases of large grains over small grains in the Hwangkeum-kong, Kwangkyo, and Bangsa-kong were 7.4%, 8.0% and 9.2%, respectively.

• The Plant Pathology Journal
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• 제18권2호
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• pp.77-80
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• 2002

A simple method for sporangial formation of the rice downy mildew pathogen, Sclerophthora macrospora, on infected leaf tissues was developed to facilitate diagnosis of the disease. Freshly infected young leaves showing whitish to yellowish small spots were selected and cut into small pieces about 2-3 cm in length. About 10-20 pieces were surface sterilized in a 100 ml Duran bottle with 40 ml of 70% ethanol by vigorous shaking for 30 seconds. After washing three times with distilled water, the leaf cuts were submerged in 10 ml of Millipore-filtered paddy water and incubated at $20^{\circ}C$ in the dark. After 8-10 h of incubation, the bottle was vigorously agitated on a vortex mixer, Aliquot amount of the suspension, 0.1-1.0 m1, was spread on a slide glass and examined under a light microscope at 50 or 100x magnification. It was found that light and 1% NaClO strongly inhibit sporangial formation of S. macrospora. Meanwhile, the use of freshly infected young loaves and washing with 70% ethanol stimulated sporangial formation of the fungus on rice leaves.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.59-65
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• 2002

ADP-glucose pyrophosphorylase (AGPase) catalyzes the key regulatory step in starch biosynthesis. Two cDNA clones encoding AGPase subunits were isolated from the leaf cDNA library of Chinese cabbage (Brassica campestris L. spp. pekinensis). One was designated as BCAGPS for the small subunit and the other as BCAGPL for the large subunit. Both cDNAs have uninterrupted open reading frames deriving 57 kDa and 63 kDa polypeptides for BCAGPS and BCAGPL, respectively, which showed significant similarity to those of other dicot plants. Also, However, the deduced amino acid sequence of BCAGPL has a unique feature. That is, it contains two regions (Rl and R2) lacking in all other plant enzymes. This is the first report of BCAGPL containing Rl and R2 among plant large subunits as well as small subunits. From the genomic Southern analysis and BAC library screening, we inferred the genomic status of BCAGPS and BCAGPL gene.

• Nuclear Engineering and Technology
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• 제49권3호
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• pp.621-631
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• 2017

Many developing countries considering the introduction of nuclear power find that large-scale reactor plants in the range of 1,000 MWe to 1,600 MWe are not grid appropriate for their current circumstance. By contrast, small modular reactors are generally too small to make significant contributions toward rapidly growing electricity demand and to date have not been demonstrated. This paper proposes a radically simplified re-design for the nuclear steam cycle for a medium-sized reactor plant in the range of 600 MWe. Historically, balance of plant designs for units of this size have emphasized reliability and efficiency. It will be demonstrated here that advances over the past 50 years in component design, materials, and fabrication techniques allow both of these goals to be met with a less complex design. A disciplined approach to reduce component count will result in substantial benefits in the life cycle cost of the units. Specifically, fabrication, transportation, construction, operations, and maintenance costs and expenses can all see significant reductions. In addition, the design described here can also be expected to significantly reduce both construction duration and operational requirements for maintenance and inspections.

• 한국산업융합학회 논문집
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• 제23권6_2호
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• pp.1081-1091
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• 2020

The most recent issue of offshore plants that produce oil and gas are the decommissioning engineering of aged or discontinued platforms. There are many platforms that are being dismantled in the United States, Europe, and areas in Southeast Asia. In particular, more than 400 old platforms in Southeast Asia (Indonesia, Malaysia) are preparing to dismantle. They are spread out across Southeast Asia with a water level of 50 meters and small-scale of less than 10,000 tons. However, this offshore plant decommissioning market is a very suitable market for small and medium-sized shipyards in Korea to enter with their established equipment and engineers. Platform decommissioning is conducted according to decommissioning procedures. However, there are some difficulties in market advances as no developed case studies or process models are established on how platform structures and components are to be dismantled and how the dismantled material is to be reused and recycled. Therefore, this study presented domestic and foreign regulations on the reuse and recycling of oil and gas producing offshore plant platforms, case analyses on developed decommissioning engineering, platform reuse and recycling guidelines, and platform and pipeline decommissioning processes and methods.

• The Plant Pathology Journal
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• 제38권5호
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• pp.432-448
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• 2022

Planthopper infestation in rice causes direct and indirect damage through feeding and viral transmission. Host microbes and small RNAs (sRNAs) play essential roles in regulating biological processes, such as metabolism, development, immunity, and stress responses in eukaryotic organisms, including plants and insects. Recently, advanced metagenomic approaches have facilitated investigations on microbial diversity and its function in insects and plants, highlighting the significance of microbiota in sustaining host life and regulating their interactions with the environment. Recent research has also suggested significant roles for sRNA-regulated genes during rice-planthopper interactions. The response and behavior of the rice plant to planthopper feeding are determined by changes in the host transcriptome, which might be regulated by sRNAs. In addition, the roles of microbial symbionts and sRNAs in the host response to viral infection are complex and involve defense-related changes in the host transcriptomic profile. This review reviews the structure and potential functions of microbes and sRNAs in rice and the associated planthopper species. In addition, the involvement of the microbiota and sRNAs in the rice-planthopper-virus interactions during planthopper infestation and viral infection are discussed.

• Journal of Forest and Environmental Science
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• 제26권1호
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• pp.9-15
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• 2010

This paper analyzes data on the plant species diversity and their contribution to the livelihoods of rural people in five villages of Domar upazila, Nilphamari district, Bangladesh. Assessment was done by means of multistage random sampling. Information collected from a total of 40 households ranging from small, medium and large categories. A total of 52 plant species belonging to 34 families were identified as being important to local livelihoods. Fruits (37%), timber (23%) and medicinal (17%) species were the most important plant use categories. Determination of the relative density of the different species revealed that Areca catechu constitutes 19.17% of homestead vegetation of the area followed by Artocarpus heterophyllus, which occupies 10.34%. Margalef index showed that there is no major difference (5.11 for large, 5.49 for medium, 4.73 for small) across the different size classes and Shannon-Weiner Index of the study area varies from 2.75 to 2.98. Results show that the average annual homestead income varied from US$108.69 to US$291.67 and contribute 6.63% of the household income.