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Isolation and Nucleotide Sequence Analysis of ADP-glucose Pyrophosphorylase gene from Chinese cabbage (Brassica rapa L.)

Kim, In-Jung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Park, Jee-Young (Department of Horticulture, College of Agriculture and Life Science, Chungnam National University)
Lee, Young-Wook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Chung, Won-Il (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Lim, Yong-Pyo (Department of Horticulture, College of Agriculture and Life Science, Chungnam National University)

Publication Information

Journal of Plant Biotechnology / v.4, no.2, 2002 , pp. 59-65 More about this Journal

Abstract

ADP-glucose pyrophosphorylase (AGPase) catalyzes the key regulatory step in starch biosynthesis. Two cDNA clones encoding AGPase subunits were isolated from the leaf cDNA library of Chinese cabbage (Brassica campestris L. spp. pekinensis). One was designated as BCAGPS for the small subunit and the other as BCAGPL for the large subunit. Both cDNAs have uninterrupted open reading frames deriving 57 kDa and 63 kDa polypeptides for BCAGPS and BCAGPL, respectively, which showed significant similarity to those of other dicot plants. Also, However, the deduced amino acid sequence of BCAGPL has a unique feature. That is, it contains two regions (Rl and R2) lacking in all other plant enzymes. This is the first report of BCAGPL containing Rl and R2 among plant large subunits as well as small subunits. From the genomic Southern analysis and BAC library screening, we inferred the genomic status of BCAGPS and BCAGPL gene.

Keywords

ADP-glurose pyrophosphorylase; cDNA cloning; Chinese cabbage (Brassica rapa L. spp. pekinensis); BAC (Bacterial Artificial Chromosome);

Citations & Related Records

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