• Biomedical Science Letters
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• v.19 no.1
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• pp.32-40
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• 2013

Pluripotent stem cells (PSCs) have enormous potential in the biomedical sciences because they can grow continuously and differentiate into any kind of cell in the body. However, for future application in regenerative medicine, it is still a challenge to control the differentiation of PSCs without using genetic materials. To control the differentiation of PSCs, small molecules might be the best substitute for genetic materials considering the following advantages: small size, which enables penetration of plasma membrane; easy-to-modify structure; and low chance of genetic recombination in treated cells. Herein, we introduce small molecules that induce the neuroectodermal differentiation of mouse embryonic stem cells (ESCs). The small molecules were identified via ESC-based consecutive screenings of small-molecule libraries composed of 324 natural compounds or 93 selected drugs. The natural compounds discovered in the first screening were used to select 93 structurally similar drugs out of 1,200 approved drugs. In the second screening, among the 93 compounds, we found 4 drugs that induced the neuroectodermal differentiation of ESCs. These drugs were progesteroneor corticoid-derivatives. Our results suggest that small molecules targeting the progesterone receptor or glucocorticoid receptor could be used as chemical tools to induce the differentiation of PSCs into a specific germ lineage.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.9-17
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• 2013

Ran is a small GTP-binding protein that binds and subsequently hydrolyzes GTP. The functions of Ran in nuclear transport and mitotic progression are well conserved in plants and animals. In animal cells, stress treatments cause Ran relocalization and slowing of nuclear transport, but the role of Ran proteins in plant cells exposed to stress is still unclear. We have therefore compared Ran genes from three EST libraries construed from different cell types of sweetpotato and the distribution pattern of Ran ESTs differed according to cell type. We further characterized two IbRan genes. IbRan1 is a specific EST to the suspension cells and leaf libraries, and IbRan2 is specific EST to the root library. IbRan1 showed 94.6 % identity with IbRan2 at the amino acid level, but the C-terminal region of IbRan1 differed from that of IbRan2. These two genes showed tissue-specific differential regulation in wounded tissues. Chilling stress induced a similar expression pattern in both IbRan genes in the leaves and petioles, but they were differently regulated in the roots. Hydrogen peroxide treatment highly stimulated IbRan2 mRNA expression in the leaves and petioles, but had no significant effect on IbRan1 gene expression. These results showed that the transcription of these two IbRan genes responds differentially to abiotic stresses and that they are subjected to tissue-specific regulation. Plant Ran-type small G-proteins are a multigenic family, and the characterization of each Ran genes under various environmental stresses will contribute toward our understanding of the distinctive function of each plant Ran isoform.

• Journal of Korean Library and Information Science Society
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• v.39 no.3
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• pp.247-265
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• 2008

One Book One City' project is regarded as one of the most successful reading promotion campaign conducted by public libraries in this age. The purpose of this research is to analyze the present stage of this campaign in Korea, and to suggest the actual plans to make this campaign successful. To look into deep context and process of this campaign in Korea, I did a case study for 'One Book One Busan' project. After analyzing the strength and weakness of the project, I suggested the strategies for the development of 'One Book One City' campaigns in Korea.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.29 no.2
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• pp.247-274
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• 2018

In 2013, the Japanese bookstore company CCC entrusted the management and operation of the library as the designated administrator of Takeo City Library. The library has a commercial facility in the hall and uses its own classification system. It was reported the number of visiting users was increased as 3.7 times in the first year of the reopening and it has attracted the attention of the public. The purpose of this study is to investigate the background, current status and operational characteristics of the Takeo City Library by conducting the literature review, content analysis of newspaper articles and interview survey on the library. As a result of the research, newspaper articles positively assessed the fact that the library provided a 'multicultural space' to small city residents, increased visitors, and local economic effects, but pointed out that the operating deficit continued, and the problem occurred in the selection of collections.

• Journal of the Korean Society for information Management
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• v.31 no.3
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• pp.89-110
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• 2014

In this study, we analyzed KERIS Document Delivery Service (DDS) using its transaction data for the period of nine years from 2004 to 2012. We first examined the overall statistics focusing on member contributions, and conducted a network analysis based on the records of request/response (supply) between member libraries. Key findings include the following: First, in over 80% of member libraries, the number of outgoing requests exceeded the number of their responses to incoming requests. That is, for the vast majority of member libraries, their participation was concentrated on the request side. Second, KERIS DDS relies heavily on a relatively small number of top contributors, especially on the supply side. While the top contributors were active in both requests and responses (supplies), in most cases, they received and processed a disproportionally large number of requests. Third, the network analysis based on DDS requests for journal articles in 2012 further revealed the central role of top contributors. The level and pattern of concentration, however, appeared to differ by subjects (DDC). Three main patterns of centralization were found in different subjects - a network centered on a single member, a network having multiple centers, or a distributed network.

• International Journal of Oral Biology
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• v.39 no.4
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• pp.169-176
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• 2014

A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format $O_{(1-6)}XXXXX-NH_2$, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-$NH_2$ (pep-1) and WXXXXN-$NH_2$ (pep-2), and one positioned peptide, RRRWRN-$NH_2$ (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic ${\alpha}$-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.181-187
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• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.

• Korean Journal of Microbiology
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• v.37 no.2
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• pp.137-144
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• 2001

In order to investigate the diversity of bacterial community in the mud flat of Sunchon Bay, Chunnam province, diversity of amplified 16S rDNA was examined. Total DNA was extracted from sediment soils and 16S rDNAs were amplified using PCR primers based on the universally conserved sequences in bacteria. Clonal libraries were constructed and 111 clones were examined by amplified rDNA restriction analysis (ARDRA) using HaeIII. Clones were clustered based on restriction patterns using computer program, GelCompar II. One hundred different RFLP types were detected from 111 clones. The 20 clones were selected and sequenced according to dendrograms derived from ARDRA, to cover most of the bacterial diversity in the clone libraries. None of the clones were identical to any representatives in the Ribosomal Database Project small subunit RNA databases and GenBank. All sequences showed between 77 and 96.8% similarity to the known 16s rRNA sequence from cultured organisms. The 20 clones sequenced fell into seven major lineages of the domain Bacteria: alpha-, delta-, gamma-Proteobacteria, low G+C Gram positive bacteria, high G+C Gram positive bacteria, Sphingobacteria (Cytophaga) and Cyanobacteria (chloroplast). Among the clones, the Proteobacteria were dominant.

• Human Ecology Research
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• v.61 no.2
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• pp.233-245
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• 2023

The aim of this study was to identify community-level childcare infrastructure for infants and toddlers and to use the data to categorize community types using K-Means cluster analysis with spatial constraints. Seven indicators of childcare resource supply were used for the purpose of categorization and the results revealed six types of community cluster. Communities in the Type 1 cluster provided sufficient parks, libraries, and kindergartens, but lacked pediatric facilities and private education institutions. This cluster comprised small cities and rural areas in Gangwon-do, Gyeongsangbuk-do, Chungcheongbuk-do, and Jeollabuk-do. The Type 2 cluster had numerous pediatric facilities and childcare centers, but lacked other childcare infrastructure. This comprised small and medium-sized cities in Gyeonggi-do, some areas in Chungcheongnam-do, Chungcheongbuk-do, and Gangwon-do bordering Gyeonggi-do. The Type 3 cluster comprised Busan, Daegu, and Gyeongsangnam-do, but had insufficient childcare infrastructure as a whole. Type 4 had the largest number of childcare centers, libraries, and private education institutions and comprised Jeollabuk-do, areas near Gwangju, and Jeju-do. Type 5, consisting of Seoul, Incheon and the southern part of Gyeonggi-do had many pediatric facilities and certified childcare centers, but lacked other childcare infrastructure. Type 6, being the rural areas and islands in Jeollanam-do, had sufficient kindergartens, but other infrastructure was insufficient. These results are expected to provide local government with policy implications in terms of relieving the childcare burden on residents with infants and toddlers.

• The KIPS Transactions:PartD
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• v.19D no.3
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• pp.247-256
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• 2012

It's not easy for medium or large sized libraries to effectively manage their vast array of books and media data. Recently, in place of magnetic stripes and barcodes, RFID technology has been applied on a small scale to simple book management and theft-prevention initiatives. The development of RFID and USN applied systems and technology has led to RFID and USN being used in a diverse range of industrial fields, including book management systems in libraries. Using the aforementioned technology, the intelligent book management system suggested in this thesis can provide a more practical, effective, content-rich and convenient book management system.