• Food Science and Preservation
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• v.23 no.7
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• pp.931-938
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• 2016

To evaluate the effect of freezing temperature on quality characteristics of dried persimmons, dried persimmons were frozen at $-50^{\circ}C$ (quick frozen, QF) and $-20^{\circ}C$ (slow frozen, SF) for 24 hr. Frozen persimmons were then stored at -20, -10, 0 and $10^{\circ}C$ for 80 days. Total free sugar content of SF persimmons was higher than those of QF in the $+10^{\circ}C$ and $-20^{\circ}C$ stored samples. Except for samples stored at $10^{\circ}C$, the CIE $L^*$ values for QF persimmons were higher than those for SF persimmons. For samples stored at $10^{\circ}C$ and $0^{\circ}C$, the CIE $a^*$ and $b^*$ values of SF samples were higher than those for QF samples. The texture of frozen dried persimmons was investigated to determine springiness, chewiness and hardness. Chewiness and hardness of samples held at $0^{\circ}C$ were higher in SF than in QF persimmons. However, when stored at $-10^{\circ}C$, chewiness and hardness were higher in QF than in SF samples. Springiness results were similar among the QF and SF persimmons held at different storage temperatures. For all storage temperatures, QF persimmons had a high soluble tannin content. All of the sample, the average soluble tannin contents of QF is 236.09 mg%. On the other hands, those of SF is 226.87 mg%. The results indicate that freezing rate and holding temperatures have significant effects on dried persimmon texture, soluble tannin level, and free sugar content. Further studies that include sensory evaluations are needed to determine the optimum freezing rate and holding temperature for dried persimmons.

• 대한생식의학회:학술대회논문집
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• 2005.11a
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• pp.131.2-132
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• 2005

• 대한생식의학회:학술대회논문집
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• 2007.11a
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• pp.129.1-129.1
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• 2007

• Journal of computational fluids engineering
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• v.7 no.4
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• pp.19-27
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• 2002

An efficient implicit multigrid method is presented for the Navier-Stokes and k-ω turbulence equations. Freezing and limiting strategies are applied to improve the robustness and convergence of the multigrid method. The eddy viscosity and strongly nonlinear production terms of turbulence are frozen in the coarser grids by passing down the values without update of them. The turbulence equations together with the Navier-Stokes equations, however, are consecutively solved on the coarser grids in a loosely coupled fashion. A simple limit for k is also introduced to circumvent slow-down of convergence. Numerical results for the unseparated and separated transonic airfoil flows show that all computations converge well without any robustness problem and the computing time is reduced to a factor of about 3 by the present multigrid method.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.31-34
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• 2002

An efficient implicit multigrid method is presented for the Navier-Stokes and $k-{\omega}$ turbulence equations. Freezing and limiting strategies are applied to improve the robustness and convergence of the multigrid method. The eddy viscosity and strongly nonlinear production terms of turbulence are frozen in the coarser grids by passing down the values without update of them. The turbulence equations together with the Navier-Stokes equations, however, are consecutively solved on the coarser grids in a loosely coupled fashion. A simple limit for k is also introduced to circumvent slow-down of convergence. Numerical results for the unseparated and separated transonic airfoil flows show that all computations converge well without any robustness problem and the computing time is reduced to a factor of about 3 by the present multigrid method.

• International Journal of Internet, Broadcasting and Communication
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• v.6 no.2
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• pp.20-24
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• 2014

These days the large-scale car accidents have often been occurred by overspeeding in disregard of sharp curve, foggy and freezing regions. This paper has proposed the algorithm to calculate the safety speed in real time that can protect the car accidents under these weather and road conditions using Fuzzy reasoning theory. Under raining and snowing, drivers have to slow down the traffic safety speed by 1/3 of the traffic safety speed indicated on the existing speed sign plate based on their decision. So it is difficult to calculate and then observe the safety speed. This paper has performed the simulation that provides the deivers with the optimal safety speed considering the road and weather conditions in real time to improve these problems. We have proved this method can improve more 25% than the existing one.

• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.97-97
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• 2002

• Geomechanics and Engineering
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• v.36 no.4
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• pp.391-406
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• 2024

In order to study the soil seasonal dynamic characteristics in the regions with four distinct seasons, the soil dynamic triaxial experiments were conducted by considering the environmental temperature range from -30℃ to 30℃. The results demonstrate that the dynamic soil properties in four seasons can change greatly. Firstly, the dynamic triaxial experiments were performed to obtain the dynamic stress-strain curve, elastic modulus, and damping ratio of soil, under different confining pressures and temperatures. Then, the experiments also obtain the dynamic cohesion and internal friction angle of the clay under the initial strain, and the changing rule was summarized. Finally, the results show that the dynamic elastic modulus and dynamic cohesion will increase significantly when the clay is frozen; as the temperature continues to decrease, this increasing trend will gradually slow down, and the dynamic damping ratio will go down when the freezing temperature decreases. In this paper, the change mechanism is objectively analyzed, which verifies the reliability of the conclusions obtained from the experiment.

• Journal of Embryo Transfer
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• v.23 no.3
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• pp.223-227
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• 2008

The cryopreservation of Hanwoo embryos has become an integral part of assisted reproduction in animal. The objective of this study was to assess the effect of The objectives of this study were: (1) to evaluate the influence of bovine embryo developmental stage on in vitro embryo development after freezing, (2) to study the efficiency compared with conventional freezed embryos at different embryo source. For conventional slow-freezing, day 7 or 8 expanded blastocysts were collected. The standard freezing medium was 1.8 M ethylene glycol (EG). Embryos were equilibrated in 1.8 Methylene glycol(EG) with 0.1 M sucrose in Dulbecco's phosphate-buffered saline (D-PBS) supplemented with 0.5% bovine serum albumin. Embryos were then loaded individually into 0.25 ml-straw and placed directly into cooling chamber of programmable freezer precooled to $-7^{\circ}C$, after 2 min, the straw was seeded, maintained at $-7^{\circ}C$ for 8 min, and then cooled to $-35^{\circ}C$ at $0.3^{\circ}C$/min, plunged and stored in liquid nitrogen for at least 3 days. For thawing, the straw containing embryos were warmed in air for 10 see and exposed to $37^{\circ}C$ water for 20 sec. Straws were then removed from $37^{\circ}C$ water. Rates of blastocyst survive and hatched were evaluated at 12 to 48h post-warming. The re-expansion and hatched rates of morula embryos were significantly lower than those obtained for blastocysts and expansion blastocysts (31.6%, 10.5% vs, 68.9%, 22.2% vs, 73.7%, 53.6%, respectively). No differences in re-expansion rates were found between in vivo and in vitro blastocysts. whereas hatched rates was significantly higher (51.2%) in vivo compared with in vitro embryos (18.6%). in conclusion, demonstrate that conventional freezing can be used successfully in cryopreservation of in vitro and in vivo bovine embryos, and that it might be considered for use in commercial programs and embryo preservation.

• Clinical and Experimental Reproductive Medicine
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• v.27 no.1
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• pp.59-65
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• 2000

Objective: The purpose of this study was to determine the important factors affecting survival and pregnancy rate in frozen-thawed embryo transfer cycles. Methods: we performed retrospective analysis in 738 cycles of frozen-thawed embryo transfers, in relation to the insemination methods, the freezing stage of embryo, patient's age, infertility factors and the origin of injected sperm in ICSI cycles. After conventional IVF or ICSI, the supernumerary PN stage zygotes or multicellular embryos were cryopreserved by slow freezing protocol with 1,2-propanediol (PROH) as a cryoprotectant. Results: The survival rates of thawed embryos were 69.3% (1585/2287) in conventional IVF group and 71.7% (1645/2295) in ICSI group. After frozen-thawed embryo transfers, 27.0% (92/341) and 32.0% (109/341) of pregnancy rates were achieved in conventional IVF and ICSI group, respectively. There were no significant difference in the survival and pregnancy rates according to the insemination methods, the freezing stage and patient's age. However, the pregnancy rate (36.2%) of male factor infertility was significantly higher than the tubal (27.2%) and other female factor infertility (22.9%). In ICSI group, the origin of injected sperm did not affect the outcome of frozen-thawed embryo transfer cycles. Conclusion: The present study demonstrates that acceptable clinical outcomes can be achieved after the transfer of frozen-thawed embryos regardless of the stage of embryos for freezing, the patient's age and the origin of injected sperm.