• Title/Summary/Keyword: Slow mixing

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Enhancement of DNA Microarray Hybridization using Microfluidic Biochip (미세유체 바이오칩을 이용한 DNA 마이크로어레이 Hybridization 향상)

  • Lee, H.H.;Kim, Y.S.
    • KSBB Journal
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    • v.22 no.6
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    • pp.387-392
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    • 2007
  • Recently, microfluidic biochips for DNA microarray are providing a number of advantages such as, reduction in reagent volume, high-throughput parallel sample screening, automation of processing, and reduction in hybridization time. Particularly, the enhancement of target probe hybridization by decrease of hybridization time is an important aspect highlighting the advantage of microfluidic DNA microarray platform. Fundamental issues to overcome extremely slow diffusion-limited hybridization are based on physical, electrical or fluidic dynamical mixing technology. So far, there have been some reports on the enhancement of the hybridization with the microfluidic platforms. In this review, their principle, performance, and outreaching of the technology are overviewed and discussed for the implementation into many bio-applications.

An Experimental Study on the Early Compressive Strength Improvement of Cement Mortar Mixed with Blast Furnace Slag using Powdered Stimulants (분말형 자극제를 이용한 고로슬래그미분말 혼입 시멘트 모르타르의 조기 압축강도 향상에 관한 실험적 연구)

  • Lee, Kang Jin;Kim, Jin Hyoung;Park, Ki Bong;Lee, Han Seung
    • KIEAE Journal
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    • v.12 no.3
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    • pp.115-122
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    • 2012
  • Based on previous research and existing literature, this study examines the development of admixture, which increases the early concrete strength (1 and 3 day) by mixing blast furnace slag cement and concrete stimulant. The research on early strength development of concrete is necessary in dealing with the drawbacks of slow early strength concrete on site and to shorten the construction time. The study confirmed that when a high alkaline mortar mixture is mixed with blast furnace slag, the early strength of admixture exceeds that of ordinary portland cement (OPC). The use of calcium chloride ($CaCl_2$) promotes hydration of cement at low temperature and show similar strength as the blast furnace slag admixture. Although calcium chloride seems economically advantageous, it causes steel corrosion and its use in concrete should be further studied in-depth.

The Flow Field Structure of Jet-in-Cross Flow through the Perforated Damage Hole (관통 손상 구멍으로부터의 제트-교차 흐름의 유동장 구조)

  • Lee, Ki-Young
    • Journal of the Korea Institute of Military Science and Technology
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    • v.17 no.4
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    • pp.551-559
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    • 2014
  • The influence of the battle damage hole on the velocity and vorticity flow field have been studied by using particle image velocimetry. Time averaged velocity and vorticity vector fields in the vicinity of jet are presented. The perforated damage hole on a wing created from a hit by anti-air artillery was modeled as a 10% chord size hole which positioned at quarter chord. At low angles of attack, the vorticity in the forward side of the jet is cancelled due to mixing with the wing surface boundary layer. Stretching of vorticity in the backside of the jet generates a semi-cylindrical vortical layer that enclosing a domain with slow moving reverse flow. Conversely, at higher the angles of attack, the jet vorticity advected away from the wing surface and remains mostly confined to the jet. The mean flow behind the jet has a wake-like structure.

Numerical research for Gate Type Waste Incinerators In Environment energy facilities (환경에너지시설내 화격자식 소각로 수치해석 연구)

  • Kim, Jong-Yoon;Jeon, Yong-Han
    • Journal of the Korea Safety Management & Science
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    • v.19 no.4
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    • pp.149-155
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    • 2017
  • This study is analyzed combustion phenomena based on the environmental energy facility incinerator. It is assumed that combustible components of waste are composed of carbon and hydrogen, and the combustion process of fuel is by setting as multi-component / multistage reaction. As the combustion chamber is burned, the high temperature environment is achieved, also the heat transfer accompanied by the turbulent flow and the generation of NOx, a pollutant, are interpreted to predict the thermal and fluid characteristics and pollution emissions of the grate incinerator. As the result of internal flow analysis, the slow flow around the ash chute and the mixing effect due to the complicated turbulence around the combustion chamber were predicted to show excellent performance. It is shown to the internal average temperature was about $1024^{\circ}C$, around the about $1000^{\circ}C$ homogeneous temperature distribution. Due to the sudden temperature decrease in the boiler, the flue gas temperature at the outlet was estimated to be about $220^{\circ}C$.

High Performance Hardware Implementation of the 128-bit SEED Cryptography Algorithm (128비트 SEED 암호 알고리즘의 고속처리를 위한 하드웨어 구현)

  • 전신우;정용진
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.11 no.1
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    • pp.13-23
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    • 2001
  • This paper implemented into hardware SEED which is the KOREA standard 128-bit block cipher. First, at the respect of hardware implementation, we compared and analyzed SEED with AES finalist algorithms - MARS, RC6, RIJNDAEL, SERPENT, TWOFISH, which are secret key block encryption algorithms. The encryption of SEED is faster than MARS, RC6, TWOFISH, but is as five times slow as RIJNDAEL which is the fastest. We propose a SEED hardware architecture which improves the encryption speed. We divided one round into three parts, J1 function block, J2 function block J3 function block including key mixing block, because SEED repeatedly executes the same operation 16 times, then we pipelined one round into three parts, J1 function block, J2 function block, J3 function block including key mixing block, because SEED repeatedly executes the same operation 16 times, then we pipelined it to make it more faster. G-function is implemented more easily by xoring four extended 4 byte SS-boxes. We tested it using ALTERA FPGA with Verilog HDL. If the design is synthesized with 0.5 um Samsung standard cell library, encryption of ECB and decryption of ECB, CBC, CFB, which can be pipelined would take 50 clock cycles to encrypt 384-bit plaintext, and hence we have 745.6 Mbps assuming 97.1 MHz clock frequency. Encryption of CBC, OFB, CFB and decryption of OFB, which cannot be pipelined have 258.9 Mbps under same condition.

Surface Ozone Episode Due to Stratosphere-Troposphere Exchange and Free Troposphere-Boundary Layer Exchange in Busan During Asian Dust Events

  • Moon, Y.S.;Kim, Y.K.;K. Strong;Kim, S.H.;Lim, Y.K.;Oh, I.B.;Song, S.K.
    • Journal of Environmental Science International
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    • v.11 no.5
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    • pp.419-436
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    • 2002
  • The current paper reports on the enhancement of O$_3$, CO, NO$_2$, and aerosols during the Asian dust event that occurred over Korea on 1 May 1999. To confirm the origin and net flux of the O$_3$, CO, NO$_2$, and aerosols, the meteorological parameters of the weather conditions were investigated using Mesoscale Meteorological Model 5(MM5) and the TOMS total ozone and aerosol index, the back trajectory was identified using the Hybrid Single-Particle Lagrangian Integrated Trajectory Model(HYSPLIT), and the ozone and ozone precursor concentrations were determined using the Urban Ashed Model(UAM). In the presence of sufficiently large concentrations of NO$\sub$x/, the oxidation of CO led to O$_3$ formation with OH, HO$_2$, NO, and NO$_2$ acting as catalysts. The sudden enhancement of O$_3$, CO, NO$_2$ and aerosols was also found to be associated with a deepening cut-off low connected with a surface cyclone and surface anticyclone located to the south of Korea during the Asian dust event. The wave pattern of the upper trough/cut-off low and total ozone level remained stationary when they came into contact with a surface cyclone during the Asian dust event. A typical example of a stratosphere-troposphere exchange(STE) of ozone was demonstrated by tropopause folding due to the jet stream. As such, the secondary maxima of ozone above 80 ppbv that occurred at night in Busan, Korea on 1 May 2001 were considered to result from vertical mixing and advection from a free troposphere-boundary layer exchange in connection with an STE in the upper troposphere. Whereas the sudden enhancement of ozone above 100 ppbv during the day was explained by the catalytic reaction of ozone precursors and transport of ozone from a slow-moving anticyclone area that included a high level of ozone and its precursors coming from China to the south of Korea. The aerosols identified in the free troposphere over Busan, Korea on 1 May 1999 originated from the Taklamakan and Gobi deserts across the Yellow River. In particular, the 1000m profile indicated that the source of the air parcels was from an anticyclone located to the south of Korea. The net flux due to the first invasion of ozone between 0000 LST and 0600 LST on 1 May 1999 agreed with the observed ground-based background concentration of ozone. From 0600 LST to 1200 LST, the net flux of the second invasion of ozone was twice as much as the day before. In this case, a change in the horizontal wind direction may have been responsible for the ozone increase.

A DLL-Based Multi-Clock Generator Having Fast-Relocking and Duty-Cycle Correction Scheme for Low Power and High Speed VLSIs (저전력 고속 VLSI를 위한 Fast-Relocking과 Duty-Cycle Correction 구조를 가지는 DLL 기반의 다중 클락 발생기)

  • Hwang Tae-Jin;Yeon Gyu-Sung;Jun Chi-Hoon;Wee Jae-Kyung
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.2 s.332
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    • pp.23-30
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    • 2005
  • This paper describes a DLL(delay locked loop)-based multi-clock generator having the lower active stand-by power as well as a fast relocking after re-activating the DLL. for low power and high speed VLSI chip. It enables a frequency multiplication using frequency multiplier scheme and produces output clocks with 50:50 duty-ratio regardless of the duty-ratio of system clock. Also, digital control scheme using DAC enables a fast relocking operation after exiting a standby-mode of the clock system which was obtained by storing analog locking information as digital codes in a register block. Also, for a clock multiplication, it has a feed-forward duty correction scheme using multiphase and phase mixing corrects a duty-error of system clock without requiring additional time. In this paper, the proposed DLL-based multi-clock generator can provides a synchronous clock to an external clock for I/O data communications and multiple clocks of slow and high speed operations for various IPs. The proposed DLL-based multi-clock generator was designed by the area of $1796{\mu}m\times654{\mu}m$ using $0.35-{\mu}m$ CMOS process and has $75MHz\~550MHz$ lock-range and maximum multiplication frequency of 800 MHz below 20psec static skew at 2.3v supply voltage.

An Experimental Study on Lean-burn Limit and Emission Characteristics of Air-fuel Ratio in a CNG Engine (수소-CNG 혼소기관의 공기과잉률 변화에 따른 희박가연한계 및 배출가스 특성에 관한 연구)

  • KIM, INGU;SON, JIHWAN;KIM, JOUNGHWA;KIM, JEONGSOO;Lee, Seong-Uk;KIM, SUNMOON
    • Transactions of the Korean hydrogen and new energy society
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    • v.28 no.2
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    • pp.174-180
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    • 2017
  • Recently, the world faces the environmental problem such as air pollution due to harmful gas discharged from car and abnormal climate due to the green-house gases increased by the discharge of $CO_2$. Compressed Natural Gas (CNG), one of alternative for this problem, is less harmful, compared to the existing fossil fuel, as gaseous fuel, and less carbon in fuel ingredients and carbon dioxide generation rate relatively favorable more than the existing fuel. However, CNG fuel has the weakness of slow flame propagation speed and difficult fast burn. On the other hand, hydrogen does not include carbon in fuel ingredients, and does not discharge harmful gas such as CO and HC. Moreover, it has strength of quick burning velocity and ignition is possible with small ignition energy source and it's has wide Lean Flammability Limit. If using this hydrogen with CNG fuel, the characteristics of output and discharge gas is improved by the mixer's burning velocity improved, and, at the same time, is possible to have stable lean combustion with the reduction of $CO_2$ expected. Therefore, this research tries to identify the characteristics of engine and emission gas when mixing CNG fuel and hydrogen in each portion and burning them in spark igniting engine, and grasp the lean combustion limit and emission gas characteristics according and use it as the basic data of hydrogen-CNG premixed engine.

Improvement of Cooling Water Quality by Coagulation and Sedimentation in Steel Mill (응집침전에 의한 제철공장 냉각수질향상)

  • Jo, Kwan-Hyung;Woo, Dal-Sik;Hwang, Byung-Gi;Lee, Seon-Ju;Park, Duck-Weon
    • Journal of Environmental Health Sciences
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    • v.35 no.5
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    • pp.411-416
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    • 2009
  • This study was initiated to improve the cooling water quality by chemical coagulation and sedimentation in steel mills. Due to the inefficient flocculation in the settling tanks of blast furnace cooling water systems, the solid particles in the cooling water overflow accumulate and clog the cooling system. To protect the cooling water system from such fouling, proper flocculants must be continuously used. Laboratory tests were performed for the indirect cooling water system of a plate mill. The batch test in the gas scrubbing cooling water system of a blast furnace showed that the proposed coagulant was more effective for the improvement of coagulation and sedimentation than the existing one. During the tests, cationic flocculants were more effective than use of only an anionic flocculant. The suggested combination of anionic and cationic flocculants can probably improve the turbidity removal efficiency of the cooling water. Proper control of the overflow rate by the designed residence time would help turbidity removal efficiency in the settling tank. In addition, the settling can be enhanced by adopting rapid and slow mixing alternatively. Scale problems in blast furnace cooling water system were reduced to some extent by efficient settling.

A Study on the Volatilization of Ammonia from Flooded Soils Mixed with Rice Straw and Liming Materials (담수토양(湛水土壤)에서 볏짚과 석회물질(石灰物質) 시용(施用)이 암모니아 휘산(揮散)에 미치는 영향(影響))

  • Oh, Wang-Keun;Hwang, Kwang-Nam;Lee, Myong-Gu
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.3
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    • pp.166-171
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    • 1982
  • A laboratiory experiment incubated at about $30^{\circ}C$ for 34 days was conducted in order to learn the effect of liming materials and rice straw on the volatilization of ammonia from flooded soils applied with urea. 1. The application of calcium hydroxide and calcium silicate increased buffer action of flood soil, though it resulted in increase in the volatilization of ammonia through raising flooded soil pH containing bicarbonate. 2. The mixing of rice straw powder to soil lowered pH of flooded soil, and decreased the volatilization of ammonia. The effect was particulary large when noliming material was used. 3. Calcium hydroxide depressed the evolution of $CO_2$ in the early days of incubation after flooding, while calcium silicate promoted the ammonification of soil nitrogen from the begining of flooding giving slow change in soil chemical properties. The rice straw was also effective in providing a favorable soil condition for the ammonification rather quickly.

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