• Journal of Environmental Science International
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• v.23 no.6
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• pp.1101-1109
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• 2014

In this study we followed biofilm formation and development in a granular activated carbon (GAC) filter on pilot-scale during the 12 months of operation. GAC particles and water samples were sampled from four different depths (-5, -25, -50 and -90 cm from surface of GAC bed) and attached biomass were measured with adenosine tri-phosphate (ATP) analysis and heterotrophic plate count (HPC) method. The attached biomass accumulated rapidly on the GAC particles of top layer throughout all levels in the filter during the 160 days (BV 23,000) of operation and maintained a steady-state afterward. During steady-state, biomass (ATP and HPC) concentrations of top layer in the BAC filer were $2.1{\mu}g{\cdot}ATP/g{\cdot}GAC$ and $3.3{\times}10^8cells/g{\cdot}GAC$, and 85%, 83% and 99% of the influent total biodegradable dissolved organic carbon ($BDOC_{total}$), $BDOC_{slow}$ and $BDOC_{rapid}$ were removed, respectively. During steady-state process, biomass (ATP and HPC) concentrations of middle layer (-50 cm) and bottom layer (-90 cm) in the BAC filter were increased consistently. Biofilm development (growth rate) proceed highest rate in the top layer of filter (${\mu}_{ATP}=0.73day^{-1}$; ${\mu}_{HPC}=1,74day^{-1}$) and 78%~87% slower in the bottom layer (${\mu}_{ATP}=0.14day^{-1}$; ${\mu}_{HPC}=0.34day^{-1}$). This study shows that the combination of different analytical methods allows detailed quantification of the microbiological activity in drinking water biofilter.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6663-6668
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• 2015

Background: Treatment failure in leukemia is due to either pharmacokinetic resistance or cell resistance to drugs. Materials and Methods: Gene expression of multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and low resistance protein (LRP) was assessed in 45 pediatric ALL cases and 7 healthy controls by real time PCR. The expression was scored as negative, weak, moderate and strong. Results: The male female ratio of cases was 2.75:1 and the mean age was 5.2 years. Some 26/45 (58%) were in standard risk, 17/45(38%) intermediate and 2/45 (4%) in high risk categorie, 42/45 (93%) being B-ALL and recurrent translocations being noted in 5/45 (11.0%). Rapid early response (RER) at day 14 was seen in 37/45 (82.3%) and slow early response (SER) in 8/45 (17.7%) cases. Positive expression of MDR-1, LRP and MRP was noted in 14/45 (31%), 15/45 (33%) and 27/45 (60%) cases and strong expression in 3/14 (21%), 11/27 (40.7%) and 8/15 (53.3%) cases respectively. Dual or more gene positivity was noted in 17/45 (38%) cases. 46.5 % (7/15) of LRP positive cases at day 14 were in RER as compared to 100% (30/30) of LRP negative cases (p<0.05). All 8 (100%) LRP positive cases in SER had strong LRP expression (p=<0.05). Moreover, only 53.3% of LRP positive cases were in haematological remission at day 30 as compared to 100% of LRP negative cases (p=<0.05). Conclusions: Our study indicated that increased LRP expression at diagnosis in pediatric ALL predicts poor response to early treatment and hence can be used as a prognostic marker. However, larger prospective studies with longer follow up are needed, to understand the clinical relevance of drug resistance proteins.

• Annals of Clinical Neurophysiology
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• v.2 no.1
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• pp.8-12
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• 2000

Purpose : The rapid development and wide popularity of Digital EEG(DEEG) is due to its convenience, accuracy and applicability for quantitative analysis. These advantages of DEEG make one hesitate to use analog EEG(AEEG). To assess the advantage of DEEG system utilizing AEEG(DAEEG) over conventional AEEG and the clinical applicability, a DAEEG system was developed and applied to animal model Methods : Sprague-Dawley rat as status epilepticus model were used for collecting the EEG data. After four epidural electrodes were inserted and connected to 8-channel analog EEG(Nihon-Kohden, Japan), continous. EEG monitoring via computer screen was done from two rats simultaneously. EEG signals through analog amplifier and filters were digitized at digital signal processor and stored in Windows-based pentium personal computer. Digital data were sampled at a rate of 200 Hz and 12 bit of resolution. Acquisition software was able to carry out 'real-time view, sensitivity control and event marking' during continuous EEG monitoring. Digital data were stored on hard disk and hacked-up on CD-ROM for off-line review. Review system consisted of off-line review, saving and printing out interesting segment and annotation function. Results: This DAEEG system could utilize most major functions of DEEG sufficiently while making a use of an AEEG. It was easy to monitor continuously compared to Conventional AEEG and to control sensitivity during ictal period. Marking the event such as a clinical seizure or drug injection was less favorable than AEEG due to slowed processing speed of digital processor and central processing unit. Reviewing EEG data was convenient, but paging speed was slow. Storage and management of data was handy and economical. Conclusion : Relatively simple digital EEG system utilizing AEEG can be set-up at n laboratory level. It may be possible to make an application for clinical purposes.

• Journal of Periodontal and Implant Science
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• v.24 no.2
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• pp.283-302
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• 1994

Gingivectomy has been utilized as a therapeutic method to remove diseased periodontal pocket wall in order to aid in root planing. Although chemical agents and electrosurgery has been used in addition to the conventional method of using surgical blades, difficulties in controling the depth of chemical penetration and effectively regenerating the gingival tissue as well as the slow wound healing has been pointed out as shortcomings of these methods. This study was designed to assess the effect of Aloe vera on wound healing of gingivectomy sites created by $CO_2$ laser on palatal gingiva of maxillary molar region of white rats. Those sites treated by surgical blades were designated as control, by $CO_2$ laser as Experimental group I, by surgical blades in addition to topical application of Aloe vera as Experimental group II, and by $CO_2$ laser and Aloe vera application as Experimental group III. Animals were sacrificed at 2 days, 3 days, 1 week, 2 weeks and 3 weeks postoperatively, and the specimens were histologically analyzed. The results were as follows : 1. Resorption of blood clots were observed in the control at 3rd day, followed by the rest of the experimental groups at 1 week postoperatively. 2. Persistent inflammation was observed up to 1 week in the control and Experimental group II and III, and until the 2nd week in Experimental group I. 3. Granulation tissue was observed up to 1 week in the control, and 2 weeks in the rest of the groups. 4. Epithelization started on the 2nd day. The control showed the most rapid epithelization, and the process was completed by the 2nd week in all groups. 5. Experimental group II and III, which were treated with Aloe vera, showed similar healing patterns to the control and Experimental group I.

• Journal of Korean Neurosurgical Society
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• v.51 no.3
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• pp.127-134
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• 2012

Objective: To analyze the prognostic factors thought to be related with survival time after a spinal metastasis operation. Methods: We retrospectively analyzed 217 patients who underwent spinal metastasis operations in our hospital from 2001 to 2009. Hematological malignancies, such as multiple myeloma and lymphoma, were excluded. The factors thought to be related with postoperative survival time were gender, age (below 55, above 56), primary tumor growth rate (slow, moderate, rapid group), spinal location (cervical, thoracic, and lumbo-sacral spine), the timing of radiation therapy (preoperative, postoperative, no radiation), operation type (decompressive laminectomy with or without posterior fixation, corpectomy with anterior fusion, corpectomy with posterior fixation), preoperative systemic condition (below 5 points, above 6 points classified by Tomita scoring), pre- and postoperative ambulatory function (ambulatory, non-ambulatory), number of spinal metastases (single, multiple), time to spinal metastasis from the primary cancer diagnosis (below 21 months, above 22 months), and postoperative complication. Results: The study cohort mean age at the time of surgery was 55.5 years. The median survival time after spinal operation and spinal metastasis diagnosis were 6.0 and 9.0 months. In univariate analysis, factors such as gender, primary tumor growth rate, preoperative systemic condition, and preoperative and postoperative ambulatory status were shown to be related to postoperative survival. In multivariate analysis, statistically significant factors were preoperative systemic condition (p=0.048) and postoperative ambulatory status (p<0.001). The other factors had no statistical significance. Conclusion: The factors predictive for postoperative survival time should be considered in the surgery of spinal metastasis patients.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1078-1087
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• 2018

Objective: The genetic diversity of the Landrace population, a representative maternal pig breed in Korea, is important for genetic improvement. Previously, the effective population size (Ne) has been used to infer the genetic diversity of a population of interest. In this study, we aimed to use single nucleotide polymorphism (SNP) data to characterize linkage disequilibrium (LD) and the Ne of the Korean Landrace population. Methods: We genotyped 1,128 Landrace individuals from three representative Korean major grand-grand-parent (GGP) farms using the Illumina PorcineSNP60 version2 BeadChip, which covers >61,565 SNPs located across all autosomes and mitochondrial and sex chromosomes. We estimated the expected LD and current Ne, as well as ancestral Ne. Results: In the Korean Landrace population, the mean LD ($r^2$) of 3.698 million SNP pairs was $0.135{\pm}0.204$. The mean $r^2$ decreased slowly with as the distance between SNPs increased, and remained constant beyond 3 Mb. According to the $r^2$ calculations, 8,085 of 3.698 million SNP pairs were in complete LD. The current Ne (${\pm}$standard deviation) of the Korean Landrace population is approximately 92.27 [79.46; 105.07] individuals. The ancestral Ne exhibited a slow and steady decline from 186.61 to 92.27 over the past 100 generations. Additionally, we observed more a rapid Ne decrease from the past 20 to 10 generations ago, compared with other intervals. Conclusion: We have presented an overview of LD and the current and ancestral Ne values in the Korean Landrace population. The mean LD and current Ne for the Korean Landrace population confirm the genetic diversity and reflect the history of this pig population in Korea.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.9
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• pp.1193-1200
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• 2001

There are many regulation test methods to be related with engine emissions such as CVS-75, D-13, ECE-15 modes and so on. Most of these modes are consisted of lots of transient conditions that have rapid acceleration, deceleration and cranking modes. In this experimental research, the engine characteristics of cranking, accelerating and power output in a S.I. engine were studied to compare with neat gasoline and alternative fuels of M30 (methanol 30%, aromatic series 32%, non-aromatic 38%) and M50 (methanol 50%, aromatic 30%, non-aromatic 20%) for performance and exhaust emissions. The results show that reformulated methanol fuels are better emissions reduction of 15.7% over than that of neat gasoline fuel especially in HC and CO emissions at cranking mode. And the accelerating performances coincide with the results of distillation curve. CO concentration for M50 fuel is varied in a just little for the condition of slow acceleration. At wide-open throttle condition, brake specific energy consumption of reformulated fuels is increased and thermal efficiency is some what lower than that of gasoline fuel.

• Journal of Sasang Constitutional Medicine
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• v.15 no.3
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• pp.22-32
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• 2003

The pulse diagnosis is the basic method of oriental medicine diagnosis. But in sasang constitutional medicine, it is said that it's not a major diagnotic method. But we don't have any sasang constitutional study of the pulse diagnosis. So I made this study by research of changing concept of the pulse diagnosis in chinese medical history and Dongyi Suse Bowon (longevity and life preservation in oriental medicine). The conclusion as follows. 1. There were many kinds of the pulse diagnosis in the ancient times, it has developed to simple and effective diagnotic method. Simple and effective is the major point of medical development, the 24 pulse is abridged to Floating Pulse(浮), Deep Pulse(沈), Slow Pulse(遲), Rapid Pulse(數) 2. The latter term of Chosun, the practical study was developed. In the view of the practical study, the pulse diagnosis has a lot of cricical point. Jung Yak-Yong, in his writing Mak Lon(脈論), criticize the pulse diagnosis. 3. In the sasang constitutional medicine, the constitutional diagnosis is very important. The methods of the constitutional diagnosis are three, the way of mind and greed, knowledge and deed, external figure and physical traits. But the pulse diagnosis is one of the way of external figure and physical traits, so we can't diagnose the exact constitution by the pulse diagnosis. 4. Dong-mu conclude that the pulse diagnosis is just the diagnostic way of symptom. But in the clinical situation, the ordinary symptoms are more important than the pulse diagnosis, because it is useful to know the condition of the ingestive food metabolism and the Qi-yack metabolism

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.6
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• pp.875-885
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• 2022

This study was performed to confirm the optimal extraction method for antimicrobial peptides from the Hard-shelled mussel. Extractions were performed with two processes including 1% HAc/boiling and 1% HAc/non-boiling methods and used extracts for the comparison of the antimicrobial activity, protease stability, action mechanism, AU-PAGE (acid-urea PAGE), and HPLC chromatograms. 1% HAc/boiling extract showed potent antibacterial activities both against Gram-positive and negative bacterium but 1% HAc/non-boiling extract showed antibacterial activity only against Gram-positive bacteria. Treatment of 1% HAc/boiling extract with proteases retained almost antibacterial activity against B. subtilis, but abolished significant antibacterial activity against E. coli D31. Only 1% HAc/boiling extract showed two discrete clearing antibacterial zones including slow migrating and rapid migrating zones. Both extracts showed strong DNA-binding ability but did not show bacterial membrane permeabilizing ability. In comparison of the chromatogram obtained from C₁₈ or cation-exchange HPLC, the eluted peaks from 1% HAc/boiling extract showed high hydrophobic property or absorbance compared to 1% HAc/non-boiling extract, respectively. The concentration of the purified antimicrobial peptide was also higher in 1% HAc/boiling extract than in 1% HAc/non-boiling extract. Our results suggest that the effective extraction condition for antimicrobial peptides from marine invertebrate is boiling process in a weak acetic acid solution (1%).

• Journal of Sasang Constitutional Medicine
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• v.18 no.1
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• pp.118-131
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• 2006

1. Objective Pulse diagnosis is generally applied to Traditional Oriental Medicine but not to Sasang Constitution diagnosis. Recently new pulse analyzer using array piezoresistive sensor and multi-channel robot arm developed. It reflects Oriental Medical Doctors' diagnostic processes, and its reproducibility test was done at Korea Institute of Oriental Medicine. We performed this study to set parameters diagnosing Sasang Constitution. 2. Methods One hundred thirty three subjects participated in this study. They are healty and approved this study. Before being tested with pulse analyzer, they had interview with Sasang Constitution Specialist to diagnose their Sasang Constitution. We established some useful parameters from parameters of pulse analyzer according to the Original Texts of Oriental Medicine and clinical experiences to analyze with clinical data of this study. 3. Results (I) There is a significant difference in pre-dicrotic notch time among all parameters of pulse analyzer in Sasang Constitution groups(P=0.047). (2) There is a significant difference in maximum pulse pressure in 33 to 48 year Sasang Constitution groups(P=0.010). (3) There is a significant difference in frequency width in 17 to 32 year Sasang Constitution groups(P=0.002). (4) There is a significant difference in CFS value in groups which OMD diagnoses; Floating & Sinking pulse(P=0.020). (5) There is a significant difference in pulse rate in groups which OMD diagnoses; Rapid & Slow pulse(P=0.000). (6) There is a significant difference in maximum pulse pressure in groups which OMD diagnoses; Deficient & Solid pulse(P=0.000). 4. Conclusions Analyzing parameters in each Sasang Constitution group, we found it shows significant difference in maximum pulse pressure and corresponding tendency in coefficient of floating & sinking pulse with theories of Sasang Consti-tutional Medicine. As we accumulate more clinical data, we will establish algorithm to diagnose Sasang Constitution using a pulse analyzer.