• Title/Summary/Keyword: Slide Method

Search Result 297, Processing Time 0.026 seconds

Morphometric Study on Fine Needle Aspirates from Follicular Adenoma and Follicular Carcinoma of the Thyroid (세침흡인된 갑상선의 여포상 선종과 여포상 암종의 형태계측학적 연구)

  • Chu, Young-Chae;Cha, Hee-Jung;Min, Soo-Kee;Kim, Joon-Mee;Hwang, Tae-Sook
    • The Korean Journal of Cytopathology
    • /
    • v.9 no.1
    • /
    • pp.63-68
    • /
    • 1998
  • Fine needle aspiration cytology of "cold" nodules of the thyroid has proved to be of great value in their preoperative diagnosis. Most types of thyroid tumors are readily recognizable from characteristic cellular patterns in the smears of needle aspirates. But follicular neoplasms present some problems because the cytomorphology of the adenomas frequently is same as in carcinoma. For differentiation of benign from malignant follicular neoplasms of the thyroid we tested the usefulness of two objective parameters - nuclear area and perimeter - by morphometry. This study was made on fine needle aspirates from 30 cases with cytologic diagnosis of follicular neoplasm of thyroid. The histologic classification was follicular adenoma in 22 cases and follicular carcinoma in 8 cases. As a reference group we used seven caes with nodular hyperplasia. The smears of aspirates were stained by Papanicolaou method. On each slide 200 randomly selected cells with intact nuclei were measured. The mean value of nuclear area are $25.32{\pm}5.50{\mu}m^2,\;34.08{\pm}7.50{\mu}m^2\;and\;39.97{\pm}6.63{\mu}m^2$ in nodular hyperplasia, follicular adenoma, and follicular carcinoma, respectively. The mean value of perimeter are $19.48{\pm}2.26{\mu}m,\;22.95{\pm}2.65{\mu}m\;and\;24.78{\pm}2.23{\mu}m$ in nodular hyperplasia, follicular adenoma and follicular carcinoma, respectively. The mean nuclear areas and perimeters of cells from follicular adenoma were significantly larger than those from nodular hyperplasia (p<0.05). The mean nuclear areas and perimeters of cells from follicular carcinoma were larger than those from follicular adenoma but the differences are not significant statistically(p>0.05). Therefore, morphometric assessment alone is inadequate to predict malignancy in thyroid aspirates.

  • PDF

Liquid-Based Cytology Using $MonoPrep2^{TM}$ System in Cervicovaginal Cytology: Comparative Study with Conventional Pap Smear and Histology (고식적 직접 도말법과 $MonoPrep2^{TM}$ system 법에 의한 자궁경부질 세포검사 성적의 비교 검토)

  • Jeon, Yoon-Kyung;Kim, Ok-Ran;Park, Ki-Wha;Kang, Soon-Beom;Park, In-Ae
    • The Korean Journal of Cytopathology
    • /
    • v.15 no.1
    • /
    • pp.33-39
    • /
    • 2004
  • We compared the diagnostic accuracy of liquid-based cervicovaginal cytology using $MonoPrep2^{TM}$ system (Monogen, Herndon, Virginia, USA), a manual system based on membrane filtration method, with conventional Pap smear. Study population included 92 patients visiting the gynecologic department under the suspicion of uterine cervical disease. In thirty of them, surgical biopsy was performed. $MonoPrep2^{TM}$ system provided well-preserved monolayer specimen with good nuclear morphology. However, about 19% of specimens were inadequate to interpret due to low cellularity. The detection rate of abnormal cells more than ASCUS (atypical squamous cells of unknown significance) was 23.9% and higher than 19.4 % of conventional Pap smear. Diagnostic concordance rate with conventional Pap smear was 81%, and severe discordance rate influencing on the management of patient was 7.6 %. Among these seven cases, $MonoPrep2^{TM}$ system was more diagnostic only in four. In comparison with histology, the sensitivity of diagnosis of $MonoPrep2^{TM}$ system was 78.9% and slightly higher than 73.5% of conventional Pap smear. However, the specificity was 81.1% and lower than 90.9% of Pap smear. In conclusion, $MonoPrep2^{TM}$ system provided diagnostic accuracies similar to the conventional Pap smear. The inexpertness of slide preparation and the low cellularity were considered to endow a limitation in more accurate evaluation.

Analysis of Factors in Visual Preference for River Scenery to estimate the Optimal Ratio of Water Surface Width.River Width - With a Focus on the Youngsan and Sumjin Rivers - (적정 수면폭.하천폭비 산정을 위한 하천경관의 시각적 선호요인 분석 - 영산강과 섬진강을 중심으로 -)

  • Yoo, Sang-Wan;Lee, Joo-Heon;Hong, Hyoung-Soon
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.35 no.1 s.120
    • /
    • pp.28-35
    • /
    • 2007
  • The purpose of this study is to evaluate the visual preference factors for river scenery, which can vary according to changes in water levels, to estimate the optimal ratio of water surface/river width. Five locations on the Youngsan and Sumjin Rivers were selected as representative samples and field survey data such as channel geometry with water levels were prepared to develop the slide of river scenery, To estimate the level of satisfaction in river scenery, slides of 4 different water levels at each of the representative locations were developed through questionnaire. To analyse the correlation between the visual preference for river scenery and preference factors, a multi regression analysis method was adopted in this study. According to the results of the multi regression analysis, Factor B(Aesthetic factors) have the greatest affect on visual preferences and Factor A(A Feeling of Open space and Physical factors) affect significantly to visual preferences for river scenery. The results of analysis shows that the most preferred W/B ratio varies from 0.5 to 0.7 and this result indicates that many people prefer high levels river flow to maintain a natural and harmonious view of rivers. The results of this study will contribute to the field of river landscape design and river restoration projects in order to maximize the human being's satisfaction as a part of nature.

Preparation of SiO2-TiO2-MxOy ( M = Co, Cr or Cu ) Thin Films by the Chemical Solution Process (스핀코팅에 의한 SiO2-TiO2-MxOy (M = CO, Cr or Cu)계 비정질 박막의 제조)

  • Kim, Sangmoon;Lim, Yongmu;Hwang, Kyuseog
    • Journal of Korean Ophthalmic Optics Society
    • /
    • v.3 no.1
    • /
    • pp.223-228
    • /
    • 1998
  • Glass films of $SiO_2-TiO_2-M_xO_y$ (M = Co, Cr or Cu) have been prepared on soda-lime-silica slide glasses by the chemical solution method using a spin-coating technique. Commercially available tetraethyl orthosilicate, titanium trichloride, and cobalt-, chromium- and copper-nitrates were used as starting materials. No crystalline segregations of $Co_3O_4$, $Cr_2O_3$ and CuO were observed by X-ray diffraction ${\theta}-2{\theta}$ scans. From the optical transmission analysis, cobalt existed as $ Co^{2+}$ in tetrahedral coordination, chromium as $Cr^{6+}$ in tetrahedral symmetry and copper as $Cu^{2+}$ in octahedral coordination. Films with a crack-free and no texture exhibited homogeneous interfaces between the films and the substrates along the cross-section.

  • PDF

Chacterization and Preparation of SiO2-TiO2-AgO thin Films by the Chemical Solution Process (용액법에 의한 SiO2-TiO2-AgO계 박막의 제조 및 특성에 관한 연구)

  • Kim, Sangmoon;Shim, Moon-Sik;Lim, Yongmu;Hwang, Kyuseog
    • Journal of Korean Ophthalmic Optics Society
    • /
    • v.3 no.1
    • /
    • pp.217-222
    • /
    • 1998
  • Coating films of $SiO_2-TiO_2-AgO$ have been prepared on soda-lime-silica slide glasses and single crystal silicon wafer by the sol-gel method using a spin-coating technique. Commercially available tetraethyl orthosilicate, titanium trichloride, and silver-nitrates were used as starting materials. The heat treatment temperature of this coating films was $500^{\circ}C$ properly, obtained from TG-DTA result. The films with thickness of 310 nm were prepared by 5 times coating. In the case of l0 mol% AgO, the film showed a crack-free and smooth surface, but the higher Ago content exhibited the more pin hole and the segregated cluster of AgO. The IR absorbance of the films decreased in the range of 400 nm to 700 nm with the increase of annealing temperature. And the reflectance of the coating films decreased and the color was changed light yellow to white yellow with the increase of Ago content.

  • PDF

Level of Safety Awareness of Construction Workers (건설현장 작업자의 내면적 안전의식 수준)

  • Lee, Wang Gi;Park, Seong Yong;Son, Ki Sang
    • Journal of the Korean Society of Safety
    • /
    • v.32 no.3
    • /
    • pp.48-53
    • /
    • 2017
  • Many construction accidents can be lead to a death occurring at construction sites. These are considerably due to potentially-hazardous equipment and machine or unexpected collapsion at earth work due to land slide and so on. Almost 50% of the total death and injury, occurred in construction sites in 2015. 66% of those serious accidents are due to falling occurring from construction sites when they work. Therefore, causes and recommendations of each accident should be deeply thought and analysed The indirect causes are directly related to safe consciousness of the construction workers. Actually, their safety consciousness are not high, even very low, it is thought. Questionnaire survey sheets have been distributed to Seoul, Incheon, and Gyunggi-Do area, first. And then, the authors have collected those directly at sites, in order to increase collection rate of the sheets. The totally, collected sheets are 295 sheets. And, they are analysed using SPSS version 19 package program. Workers internal consciousness has been investigated and reviewed and analysed by statistical method such as frequency rate, crossed, and correlated analysis. And finally the conclusions for the above analyses are as follows; Heavy weight worth a crew of more than two workers should be necessarily considered for the advanced safety plan and needed for making a highly potential hazard group at construction sites. Safety consciousness, earing p.p.e, workman ship should be mainly considered for investing safety costs with an aspect of human factor.

Modified Lorenz Chaos Synchronization Via Active Sliding Mode Controller (능동 슬라이딩 모드 제어기를 이용한 변형된 Lorenz 카오스 동기화)

  • Ryu, Ki-Tak;Lee, Yun-Hyung
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.19 no.7
    • /
    • pp.16-23
    • /
    • 2018
  • Chaos is one of the most significant topics in nonlinear science, and has been intensively studied since the Lorenz system was introduced. One characteristic of a chaotic system is that the signals produced by it do not synchronize with any other system. It therefore seems impossible for two chaotic systems to synchronize with each other, but if the two systems exchange information in just the right way, they can synchronize. This paper addresses the problem of synchronization in a modified Lorenz chaotic system based on active control, sliding mode control, and the Lyapunov stability theory. The considered synchronization scheme consists of identical drive and response generalized systems coupled with linear state error variables. For this, a brief overview of the modified Lorenz chaotic system is given. Then, control rules are derived for chaos synchronization via active control and slide mode control theory, with a strategy for solving the chattering problem. The asymptotic stability of the overall feedback system is established using the Lyapunov stability theory. A set of computer simulation works is presented graphically to confirm the validity of the proposed method.

Comparison of diagnostic methods for detection of Brucella species in dog blood samples (개 혈액 재료에서의 Brucella 검출을 위한 진단방법의 비교)

  • Kwon, Soon-Oh;Lam, Truong Quang;Her, Moon;Ahn, Dong-Chun;Park, Sang-Hee;Park, Mi-Yeoun;Lee, Young-Ju;Hahn, Tae-Wook
    • Korean Journal of Veterinary Service
    • /
    • v.32 no.4
    • /
    • pp.335-341
    • /
    • 2009
  • Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

Slope Stability Analysis of Unsaturated Soil Slopes Due to Rainfall Infiltration (강우침투에 따른 불포화 토사사면의 안정해석)

  • 조성은;이승래
    • Journal of the Korean Geotechnical Society
    • /
    • v.16 no.1
    • /
    • pp.51-64
    • /
    • 2000
  • This paper presents a procedure of calculating a safety factor of the unsaturated slope suffering from the rainfall infiltration. The process of infiltration into a slope due to rainfall and its effect on the behavior of the soil slope are examined by using a two dimensional finite element flow-deformation coupled analysis. A factor of safety is calculated at various elapsed times after the commencement of rainfall as in the following procedure. First, stresses are estimated at each Gaussian point from the coupled finite element analysis. Then, the global stress smoothing method is applied to get a continuous stress field. Based on this stress field, a factor of safety is calculated for a specified slip surface by a stress integration scheme. Then, a search strategy is used to find out a critical slip surface which is associated with the minimum factor of safety. Some numerical examples are analyzed in order to study the effect of hydraulic conductivity on the slope stability during rain-induced infiltration. According to the results, local failure zone can be formed near the slope surface due to inhomogeneous distribution of hydraulic conductivity If the failure zone is once formed, then the region extends until a large amount of slide activates. Therefore the local failure can be neglected no longer in the stability analysis.

  • PDF

The contamination check before inoculation at the liquid Spawn on Flammulina velutipes (팽나무버섯 액체 종균의 접종 전 오염 검사)

  • Shim, Kyu-Kwang;Yoo, Young-Jin;Koo, Chang-Duck;Kim, Myung-Koon
    • Journal of Mushroom
    • /
    • v.10 no.1
    • /
    • pp.44-48
    • /
    • 2012
  • In this study, whether Giemsa staining solution can accurately determine bacterial contamination of liquid spawn for Flammulina velutipes in a short period of time was investigated. Giemsa solution staining cells of blood, bone marrow, lymph node, malaria parasites, rickettsia et al. was prepared by dissolving basic methylene azul and methylene blue, and acidic eosine in methyl alcohol-glycerine. Supernatant samples of Flammulina velutipes liquid spawn cultured under explosive aeration were placed on a slide, mixed with Gimesa solution and examined with optical microscope after staining. In 40 to 60 seconds bacterial cells were distinguishable from soybean meal residual and hyphal cell fragments. Thus we conclude that microscopy using Gimesa staining solution is a quick, simple and accurate method for the mushroom growers to effectively use to detect bacterial contamination of the liquid spawn.