• 한국식품과학회지
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• 제25권1호
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• pp.57-62
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• 1993

본 연구는 사후 저장온도 $15^{\circ}C$가 한우근육(M. sternomandibularis)의 생화학, 물리학적 변화에 미치는 영향을 조사하였다. 도살 1.5시간 후의 pH는 6.7/6.8이었으며, 저장온도 $15^{\circ}C$에서 $18{\sim}20$시간 후 최종 pH인 5.5에 도달했다. 한우육의 불가역적인 신전성의 상실 즉, 사후강직은 $15^{\circ}C$에서 도살 $9{\sim}10$시간 후 pH 6.1{\sim}6.0$, R-value $1.05{\sim}1.10$에서 시작하여 도살 $18{\sim}20$시간, pH 5.9에서 강직이 완료되어 신전성을 완전히 상실하였다. 저장온도 $15^{\circ}C$에서 한우근육은 약 20%, 근절은 15% 정도 수축하였다. 한우육은 도살 약 1일 후 전단력이 4.7 kg으로 가장 높았으며, 9일 숙성 후 3.0 kg으로 숙성기간이 경과함에 따라 연도가 증가하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.1011-1017
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• 2002

Conjugated linoleic acids (CLA) have been shown to decrease body fat content of individually-housed pigs but little is known about the responses under commercial conditions. Two studies were conducted to evaluate the effect of CLA under commercial conditions using contemporary genotypes. The experimental designs were similar between the two sites. Briefly, the studies were 2${\times}$2 factorial designs with the respective factors being sex (boar and gilt) and supplemental dietary CLA (0 and 4 g/kg). The studies involved 16-20 pens of pigs with 4-5 pens of each sex${\times}$CLA group. The first study was conducted with 144 pigs in 16 pens consuming a pelleted feed for 6 weeks at Bunge Meat Industries, Corowa, NSW. In the second study, 160 pigs were obtained from a commercial source and put into 20 pens in simulated commercial conditions and fed a mash diet for 7 weeks at Medina Research Station, WA. In Study 2 some aspects of meat quality were also investigated. Data from Study 1 showed that, although CLA had no significant effect upon feed intake and daily gain, the small changes in both resulted in a reduction in (-0.10 g/g, p=0.10) feed conversion ratio (FCR). While there was no significant effect of CLA on ultrasonic backfat depths, there was a significant decrease in carcass P2 (-1.0 mm, p=0.014) and estimated carcass fat (-7 g/kg, p=0.049). In the study conducted at Medina CLA had no significant effect upon feed intake, feed:gain or most measures of back fat. The exception was that dietary CLA decreased the rate of accumulation of fat at the shoulder, particularly in gilts, resulting in a significantly lower amount of shoulder fat at slaughter (-1.3 mm, p=0.044). CLA tended to increase dressing percentage although this was not significant (+0.5%, p=0.14). Meat from CLA treated pigs tended to be darker (p=0.12) and had a higher ultimate pH (p=0.06). These data suggest that under commercial conditions dietary CLA can improve growth performance and decrease P2 in pigs of an improved genotype, particularly gilts.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.26-33
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• 2002

In order to assess the effect of feeding raw or processed cotton (Gossypium) seed meal (CSM) on meat and wool production, 30 male crossbred lambs (3-4 months) of uniform body weight were assigned equally to five dietary treatments in a completely randomised design. The CSM was processed by three different methods i.e., cooking the meal at $100^{\circ}C$ for 45 minutes, treatment with 1% calcium hydroxide ($Ca(OH)_2$) for 24 h and iron treatment in the ratio of 1 part free gossypol (FG) to 0.3 parts of iron for 30 minutes. The lambs were fed isonitrogenous and isocaloric concentrate mixtures, containing 30% deoiled peanut meal (reference diet) and 40% of either raw, cooked, $Ca(OH)_2$ or iron treated CSM for 180 days. The raw and variously processed CSM replaced about 50% nitrogen of reference concentrate mixture. The concentrate mixtures were fed to meet 80% of the protein requirements (NRC, 1985) along with ad libitum chopped maize (Zea mays) hay. The slaughter weight, empty body weight and carcass weight was higher ($p{\leq}0.01$) in lambs fed cooked CSM incorporated diets, compared to diets containing deoiled peanut meal (DPNM). These parameters were not influenced by feeding diets containing either raw, $Ca(OH)_2$ or iron treated CSM in comparison DPNM diets. The carcass length, loin eye area and edible and inedible portion of carcass and the meat: bone ratio in whole carcass were also not affected by feeding CSM based diets. Among various primal cuts, the yield of legs was lower ($p{\leq}0.05$) from raw CSM fed lambs in comparison to DPNM fed lambs. The fat content in the Longissimus dorsi muscle was reduced ($p{\leq}0.05$) in lambs fed processed CSM based diets compared to those fed DPNM diet. Replacing DPNM with either raw or processed CSM based diets did not influence the sensory attributes and overall acceptability of meat. The wool yield was higher ($p{\leq}0.05$) in iron treated CSM fed lambs. The fibre length and fibre diameter were comparable among lambs on various dietary regimes. Among lambs fed variously processed CSM diets, the feed cost per kg of edible meat production was lower ($p{\leq}0.05$) on $Ca(OH)_2$ treated CSM, followed by cooked CSM diet and then on raw CSM based diets compared to DPNM diet. The CSM after 1% $Ca(OH)_2$ treatment or cooking for 45 minutes appears to be a satisfactory protein supplement in lamb diets for meat and wool production to replace at least 50% nitrogen of scarce and costly peanut meal.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.155-160
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• 2000

In order to understand the effects of sex or age on cellular characteristics of adipocytes from Hanwoo and sheep, samples were obtained from omental, subcutaneous, intermuscular and intramuscular adipose tissue depots of bulls, steers, heifers and cows in Hanwoo, and perirenal, omental and subcutaneous adipose tissues of fetal lambs, suckling lambs and wethers in sheep. In case of Hanwoo, mean diameter, surface area and volume of adipocytes from each depot were obtained by multisizer II (Coulter Co., UK). Osmium-fixed adipocytes were sized and counted using $560{\mu}m$ aperture. For samples obtained from sheep, cellularity was measured by using microscope and MCV program of Texas Instrument. Bulls had less subcutaneous and kidney fat than steers even though their slaughter and carcass weight were heavier. The amounts of fat from cows were greater in subcutaneous, kidney and internal organs than heifers. Steers had larger adipocytes in subcutaneous, intermuscular and intramuscular adipose tissues than bulls, although the differences were significant only for the subcutaneous adipose tissue depots. Adipocytes appeared to be largest in omental and smallest in intramuscular adipose tissue, although there were no significant differences among tissues. In a comparison of heifers and cows, significant site effects (p<0.05) were shown in adipocyte diameter, surface area and volume, and adipocyte appeared to be largest in omental tissue. Statistical difference (p<0.05) was only shown in cell volume of intramuscular tissue which was higher in cow than heifer. Intramuscular adipose tissue tended to have relatively greater numbers of cells per gram tissue and reflect lesser maturity of intramuscular adipose tissue relative to other adipose tissues. In sheep, regardless of adipose tissue depots, wethers had the greater adipocyte diameters than those at any other growth stage of sheep. Within adipose depots, the ranking of cell size was the greatest in the omental tissue of wether and the lowest in the renal and subcutaneous adipose tissue depots of fetal lamb. The cell size of adipocyte became larger with age, especially from fetal to suckling lamb due to a rapid hypertrophy of both perirenal and subcutaneous adipocytes during the suckling period.

• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1437-1449
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• 2006

Thirty six pre-weaned Barbari kids at 4 months age were reared on four rations computed using coarse cereal grains and by-products of pulses and oil seeds with Crude Protein (CP) and Total Digestible Nutrients (TDN) of 12 and 55% (Low protein Low energy); 12 and 60% (Low protein High energy); 14 and 55% (High protein Low energy); and 14 and 60% (High protein High energy), respectively. After 180 days on feed, male animals ($4{\times}5=20$) were slaughtered to study the effect of diet on carcass characteristics and meat quality. To asses the effect, if any, of such diet on product quality, meat balls were prepared and evaluated for quality changes when fresh as well as during storage ($-20{\pm}1^{\circ}C$). Feeding a ration with CP12 and TDN 60% (LH) to kids produced animals with highest slaughter weight (20.3 kg) yielding higher carcass weight and dressing percentage, lean (65.6%) and fat (6.6%) contents with low bone and trim losses. Although total variety meat yield was markedly higher in HL, the non-carcass fat deposition was relatively higher in LH carcasses. The water activity ($a_w$) of fresh goat meat ranged from 0.994-0.995 and total cholesterol 72.8-90.5 mg/100 g meat. The pH was high in HL and HH meat resulting in decreased ($p{\leq}0.05$) extract release volume (ERV). Meat balls were prepared using meat obtained from goats fed different rations (treatments) and stored at $-20{\pm}1^{\circ}C$. They were evaluated on day 0 and months 1, 2, 3, 4 for physicochemical, microbiological and organoleptic changes. Overall moisture (%), $a_w$, TBA number and pH value were 67.9, 0.987, 0.17, 6.6 respectively and were not affected by treatments except pH that was significantly ($p{\leq}0.01$) lower on LH. As the storage period advanced moisture, pH, $a_w$ and TBA number increased irrespective of treatments. Feeding various diets had no marked effect on microbial load of meat balls but with increasing storage period Standard Plate Count (SPC) and psychrotrophs declined ($p{\leq}0.01$). Treatment LL and LH produced meat balls with better flavour.

• Safety and Health at Work
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• 제8권2호
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• pp.198-205
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• 2017

Background: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. Methods: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of $Fc{\gamma}$ receptor I (CD64), $Fc{\gamma}RIII$ (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay. Results: Compared with the controls, ragpickers had significantly (p < 0.0001) higher levels of CD8-T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group (p < 0.0001), but their absolute number was relatively unchanged (p = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls (p < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes. Conclusion: Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and $Fc{\gamma}$ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).

• 한국가금학회지
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• 제45권2호
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• pp.81-88
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• 2018

본 연구에서는 농산부산물의 사료첨가 급여가 육계의 생산성과 혈액성상에 미치는 효과를 메타분석이라는 통계적 방법을 이용하여 조사하였다. 본 분석에 사용된 연구논문들은 모두 대한민국에 소재한 일반 상업 육계농가에서 사양시험을 수행하였고, 농산부산물의 사료첨가 급여 효과를 조사하였다. 총 11개의 연구논문에서 32개의 실험들을 메타분석에 이용하였다. 사양 단계는 총 사육일수를 기준으로 두 단계로 구분하였고, 생산성으로는 체중, 증체량, 사료섭취량 그리고 사료요구율을 평가하였다. 그리고 도체중, 도체율, 간장 및 비장 무게에 대한 효과를 평가하였다. 혈액성상으로는 혈중 포도당, 콜레스테롤, 중성지방, 고밀도지방단백 및 저밀도 지방단백함량에 대한 효과를 조사하였다. 농산부산물의 사료첨가 급여는 육계의 출하체중을 유의적으로 증가시키는 결과를 나타내었다. 사료요구율도 농산부산물을 사료첨가하여 급여한 처리구에서 유의적으로 낮게 나타났다. 즉, 농산부산물의 사료첨가급여는 육계 생산성을 개선할 수 있는 가능성이 존재함을 확인할 수 있었다. 도체중, 도체율, 간장 및 비장 무게에서는 유의적인 처리효과를 관찰할 수 없었으나, 복강지방은 농산부산물 급여 처리에 의해 유의적으로 감소하는 것을 확인하였다. 혈액성상에 있어서는 총 콜레스테롤 함량 및 중성지방 수치가 처리효과에 의해 유의적으로 감소하였고, 고밀도지방단백은 유의적으로 증가하였다. 일련의 결과를 통하여 농산부산물의 사료첨가 급여는 육계의 혈액성상 개선과 복강내 지방 침착 억제를 통한 생산성 개선의 효과를 갖는 것을 알 수 있었다.

• 한국방사선학회논문지
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• 제14권5호
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• pp.651-660
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• 2020

본 연구에서는 보급형 3D 프린터를 이용하여 인체 Femur와 유사한 HU 값을 가진 팬텀을 제작하여 기존 돼지 뼈를 대체할 수 있는지 분석하고자 하였다. 인체 Femur의 HU 값을 알아보기 위해 연령별 총 372명의 데이터를 분석하였다. 보급형 3D 프린터를 이용하여 PLA-Cu 20%를 이용하여 인체 뼈 모형 팬텀을 제작하여 CT 검사하였다. 돼지 뼈는 생후 6개월 된 돼지로 도축된 지 2일이 지난 뼈를 이용하였다. 검사결과 내부채움 80%로 제작한 3D 프린팅 팬텀이 인체의 모든 데이터와 유사한 값이 나타났고(p<0.05) 돼지뼈와는 차이가 있었다(p>0.05). 또한 연령대별 Femur의 HU 값의 경우 연령대가 증가할수록 HU의 값은 줄어드는 것으로 확인 되었다(p<0.05). 3D 프린팅과 HU 값은 적층 높이에 대해서는 약한 음의 상관성을 확인 하였지만 내부 채움에서는 182.13±1.290으로 강한 양의 상관성(R²=0.996)을 확인하였다(p<0.05). 결론적으로 3D 프린팅을 이용한 인체 모형 팬텀이 기존 돼지뼈 팬텀에 비해 인체와 유사한 정도의 HU 값을 나타낼 수 있음을 확인할 수 있었으며 이에 본 연구가 3D 프린터를 이용한 인체 모형 팬텀의 제작에 기초자료를 제공할 수 있을 것이라 사료된다.

• 한국축산식품학회지
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• 제31권5호
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• pp.748-755
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• 2011

팜유의 급여가 한우의 도체성적 및 육질에 미치는 영향을 구명하고자 실시하였다. 30개월령 거세 한우를 팜유의 급여(100 g/head/d) 유무에 따라 3개월 동안 공시하였으며, 시료는 등심(M. longissimus) 부위를 $4^{\circ}C$에 9일 동안 저장하였다. 도체성적, 총육색소 함량, pH, 총환원력 및 근소편화지수는 팜유의 급여에 따른 차이가 없었다. 일반성분 함량은 팜유 급여구의 조지방 함량이 대조구에 비해 높았으며(p<0.05), 이로 인해 보수력과 연도는 팜유 급여구가 높았다(p<0.05). 지방산 조성은 팜유 급여구에서 대조구에 비해 높은 palmitic acid(C16:0) 함량과 낮은 다가 불포화지방산(PUFA) 함량 을 보였다 (p<0.05). 저장 중 품질은 팜유 급여구가 대조구보다 높은 지방산화 안정성, 육색소산화 안정성 및 육색 안정성을 보였다. 따라서 한우에게 팜유를 급여했을 때 쇠고기의 마블링 및 저장성은 증가되었으나, 다가불포화지방산은 감소되었다.

• 한국축산식품학회지
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• 제27권3호
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• pp.351-356
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• 2007

본 연구는 포유류의 Y-염색체상에 존재하는 SRY 및 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 PCR 기법으로 쇠고기 성판별 기술을 개발하고자 수행하였다. 성 결정 유전자 영역의 특정 염기서열을 포함하는 primer를 각각 설계 합성하고 이들 primer를 이용하여 PCR증폭을 실시한 다음, 각각의 증폭산물을 1.5% agarose gel에 전기영동하여 웅성 특이적 DNA band의 증폭여부를 확인하였다. SRY 유전자에서 웅성개체 쇠고기는 1,348 bp 크기의 단편을 가진 DNA band가 검출되었으나, 자성개체의 경우 DNA band가 전혀 검출되지 않은 것을 확인 할 수 있었다. 또한, ZFY 유전자에서 웅성개체의 쇠고기는 979 bp 크기의 단편을 가진 DNA band가 모두 검출되었으나, 자성개체의 쇠고기에서는 역시 DNA band가 전혀 검출되지 않았다. 즉, SRY 및 ZFY 유전자는 모두 숫소에서 유래한 쇠고기에서 웅성 특이적인 DNA band가 정확히 검출된 반면 암소에서 유래한 쇠고기에서는 웅성 특이적 DNA band가 전혀 검출되지 않았다. 또한 쇠고기 성 감별법을 도축 후 가공 유통판매단계에 실제 활용 가능성을 검증하고자 시중에 유통되고 있는 등심 및 갈비 포장육 350시료를 무작위 추출하여 성 판별을 실시한 결과 숫소가 252시료(72%) 그리고 암소가 98두(28%)로 조사되었다. 따라서 본 연구에서 개발한 SRY 또는 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 쇠고기 성 감별기술은 생산단계는 물론 도축 후 가공 유통 판매되고 있는 모든 쇠고기의 암수 성감별을 위한 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다.