• IMMUNE NETWORK
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• 제8권3호
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• pp.82-89
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• 2008

Background: Although a skin test is the primary option for detecting allergen-specific IgE in clinics, the serum IgE immunoassay is also important because it allows for the diagnosis of allergy without any accompanying adverse effect on the patient. However, the low detection limit of IgE levels by immunoassay may restrict the use of the method in some occasions, and improving its sensitivity would thus have a significant implication in allergy-immunology clinics. Methods: In this study, we attempted to detect specific serum IgE by using immuno-polymerase chain reaction (IPCR) which combines the antigen-antibody specificity of enzyme-linked immunosorbent assays (ELISAs) with the amplification power of PCR. Results: Our results demonstrated that Blo t5-specific serum IgE can be detected by IPCR with a 100-fold higher sensitivity than ELISA, and cross-reactivity of serum IgE to other mite allergens is able to be analyzed by using only $0.3{\mu}l$ of serum sample. Use of real-time IPCR seemed to permit more convenient determination of specific serum IgE as well. Conclusion: We believe that IPCR can serve as a valuable tool in determining specific serum IgE, especially when the amount of serum sample is limited.

• 한국콘텐츠학회논문지
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• 제6권12호
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• pp.105-112
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• 2006

본 연구에서는 일반적으로 잘 알려진 기저 세포암 검출을 위한 간단한 전처리 방법을 제안하였다. 전처리 과정은 half Hanning 윈도우와 함께 데이터를 클리핑하고 PCA(principal components analysis)를 이용하여 차원을 감소하였다. Half Hanning 윈도우는 $1650cm^{-1}$ 피크 부근의 크기를 낮춤으로써 음성 오류율을 줄여 분류 성능을 향상시켰다. 이 실험에서 사용한 MAP(maximum a posteriori), KNN (k-nearest neighbor), PNN(probabilistic neural network), MLP(multilayer perceptron), SVM(support vector machine)와 MSE(minimum squared error)의 분류결과는 제안한 방법이 효과적임을 입증하고 있다. KNN 분류방법은 216개 라만 스펙트럼에 대한 분류실험에서 민감도가 약 97.3%로 제안한 윈도우를 적용한 이 실험에서 기저 세포암 검출 성능이 가장 많이 개선되었다.

• 공업화학
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• 제32권5호
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• pp.556-561
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• 2021

최근 산화철 나노입자는 외부의 자기장에 반응하는 자성의 특성과 생체적합성이 뛰어나 약물전달시스템(drug delivery system, DDS)에 관한 많은 연구의 소재로 사용되어져 왔다. 본 연구에서는 마이크로니들(microneedles, MNs)의 매트릭스 물질로 HA (hyaluronic acid)와 CMC (carboxy methyl cellulose)를 이용하여 SIONs (superparamagnetic iron oxide nanoparticles)이 함유된 HA-SMNs (hyaluronic acid-superparamagnetic microneedles)와 CMC-SMNs (carboxy methyl cellulose-superparamagnetic microneedles)를 제조하였으며, SEM (scanning electron microscopic), SQUD-VSM (superconducting quantum interference device-vibrating sample magnetometer), FMMD (frequency mixing magnetic detection), 고분자 및 바이오 멤브레인을 이용하여 SMNs의 다양한 특성을 조사하였다. SQUID-VSM 측정 결과 SIONs이 포함된 HA-SMNs와 CMC-SMNs에서 초상자성의 특성이 나타났으며, FMMD 측정에서는 SIONs 농도가 증가함에 따라 신호 강도에 변화가 확인되었다. 또한 SMNs의 바이오 막을 통한 HA-SMNs와 CMC-SMNs의 투과도 분석에서는 각각 평균 92.5%와 98.5%의 피부 투과율이 조사되었다. 이러한 결과를 통해 SMNs 제형은 경피약물전달시스템(transdermal drug delivery system, TDDS) 및 MR(magnetic mesonance) molecular imaging 분야의 전달소재로 활용될 수 있을 것으로 기대한다.

• 한국정보기술학회 영문논문지
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• 제10권1호
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• pp.71-83
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• 2020

In this paper, we propose a system that can detect the shape of a hand at high speed using an FPGA. The hand-shape detection system is designed using Verilog HDL, a hardware language that can process in parallel instead of sequentially running C++ because real-time processing is important. There are several methods for hand gesture recognition, but the image processing method is used. Since the human eye is sensitive to brightness, the YCbCr color model was selected among various color expression methods to obtain a result that is less affected by lighting. For the CbCr elements, only the components corresponding to the skin color are filtered out from the input image by utilizing the restriction conditions. In order to increase the speed of object recognition, a median filter that removes noise present in the input image is used, and this filter is designed to allow comparison of values and extraction of intermediate values at the same time to reduce the amount of computation. For parallel processing, it is designed to locate the centerline of the hand during scanning and sorting the stored data. The line with the highest count is selected as the center line of the hand, and the size of the hand is determined based on the count, and the hand and arm parts are separated. The designed hardware circuit satisfied the target operating frequency and the number of gates.

• 비파괴검사학회지
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• 제29권6호
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• pp.591-602
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• 2009

The Compton backscatter technique has been applied to lap-joint in aircraft structure in order to determine mass loss due to exfoliative corrosion of the aluminum alloy sheet skin. The mass loss of each layer has been estimated from Compton backscatter A-scan including the aluminum sheet, the corrosion layer, and the sealant. A Compton backscattering imaging system has been also developed to obtain a cross-sectional profile of corroded lap-splices of aging aircraft using a specially designed slit-type camera. The camera is to focus on a small scattering volume inside the material from which the backscattered photons are collected by a collimated scintillator detector for interpretation of material characteristics. The cross section of the layered structure is scanned by moving the scattering volume through the thickness direction of the specimen. The theoretical model of the Compton scattering based on Boltzmann transport theory is presented for quantitative characterization of exfoliative corrosion through deconvolution procedure using a nonlinear least-square error minimization method. It produces practical information such as location and width of planar corrosion in layered structures of aircraft, which generally cannot be detected by conventional NDE techniques such as the ultrasonic method.

• 한국잠사곤충학회지
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• 제39권2호
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• pp.146-153
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• 1997

The microsporidian infection with Nosema bombycis, reconfirmed its high virulence and transovarial tranmissibility, however, the characteristic symptom of the spots like pepper grains on the diseased larval skin was no more recognized by present varieties of the silkworm. Transovarial transmission rate detected from moth was above 90% in dead eggs or dead larvae in the rearing by mulberry leaves, 80% in the newly hatched larvae starved to death. Transovarially transmitted N. bombycis was easily observed from dead eggs and larvae, and were suggested an individual inspection of a few of dead eggs for detection of the pathogenic spores. The progeny population provided indicative factors on the sampling of predictive and corrective inspection. The higher concentration of N. bombycis spores included in the hindabdormal part of infected moth, applicative on the simple method of indivisual moth inspection. For the predictive inspection of growned 5th-instar larvae, N. bombycis infection was detectable without microscopic observation by the unique symptom of turbid milky-white spots on the silk gland. Inspection of the meconia artificially discharged from silkworm moth, was also succesful of microscopic observation before crossing, without killing or homogenize the moths. The results provided a basis of rational methods for the inspection of N. bombycis infection of the silkworm.

• 한국멀티미디어학회논문지
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• 제8권4호
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• pp.461-472
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• 2005

본 논문에서는 스테레오 영상의 정합값(matching)을 통한 얼굴 특징추출 알고리즘을 제안한다. 제안된 알고리즘에서는 얼굴색상 정보의 RGB컬러공간을 YCbCr컬러공간으로 변환하여 얼굴영역 검출하였다. 추출된 얼굴영역으로부터 눈 형판(template)을 적용하여 눈 사이의 거리와 기울어짐, 코와 입에 대한 특징의 기하학적인 특징 벡터를 추출하였다. 또한 제안한 방법은 2차원 특징정보 뿐만 아니라 스테레오 영상의 정합을 통한 얼굴의 눈, 코, 입의 특징을 추출할 수 있었다. 실험을 통하여 약 1m이내 거리에서 73%의 일치율을 보였고, 약 1m이후 거리에선 52%의 일치율을 보였다.

• Toxicological Research
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• 제17권
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• pp.59-69
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• 2001

Arsenic exposure is associated with several human diseases, including cancers, atherosclerosis, hypertension, and cerebrovascular diseases. In cultured cells, arsenite, an inorganic arsenic com-pound, was demonstrated to interfere with many physiological functions, such as enhancement of oxidative stress, delay of cell cycle progression, and induction of structural and numerical changes of chromosomes. The objective of this study is to investigate the effects of arsenic exposure on gene expression profiles by colorimetric cDNA microarray technique. HFW (normal human diploid skin fibroblasts), CL3 (human lung adenocarcinoma cell line), and HaCaT (immortalized human keratinocyte cell line) were treated with 5 $\mu\textrm{M}$ or 10 $\mu\textrm{M}$ sodium arsenite for 6 or 16 h, respectively. By a dual-color detection system, the expression profile of arsenite-treated cultures was compared to that of control cultures. Several genes expressed differentially were identified on the microarray membranes. For example, MDM2, SWI/SNF, ubiquitin specific protease 4, MAP3K11, RecQ protein-like 5, and Ribosomal protein Ll0a were consistently induced in all three cell types by arsenite, whereas prohibitin, cyclin D1, nucleolar protein 1, PCNA, Nm23, and immediate early protein (ETR101) were apparently inhibited. The present results suggest that arsenite insults altered the expression of several genes participating in cellular responses to DNA damage, stress, transcription, and cell cycle arrest.

• The Korean Journal of Pain
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• 제18권2호
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• pp.210-213
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• 2005

Acute viral meningitis and myositis are rare complications of varicella-zoster virus (VZV) reactivation. A 71-years-old immunocompetent man, who presented with lower back pain radiating to the left lower extremities, developed vesicles on the L5 dermatomal area. The next day, he had complained of aberrant vesicles on the trunk, face and scalp, with generalized myalgia, headache and dizziness. He was confirmed with VZV meningitis and myositis, as demonstrated by the presence of VZV DNA in the blood and cerebral spinal fluid using a polymerase chain reaction (PCR) amplification. PCR has been used in patients with a VZV infection associated neurological symptoms, and provides a useful tool for the early diagnosis of VZV-associated neurological disease. The patient was treated with bed rest, with intravenous acyclovir for the VZV infection, and intravenous Patient-controlled Analgesia for pain management and the prevention of postherpetic neuralgia. When he visited the outpatient department 3 months later, the skin lesion, leg pain, headache and myalgia had all improved, without sequelae. Here, this case is reported, with a discussion of the relevant literature on its diagnosis and management.

• 로봇학회논문지
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• 제10권3호
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• pp.171-177
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• 2015

A telerobot offers a more engaging and enjoyable interaction with people at a distance by communicating via audio, video, expressive gestures, body pose and proxemics. To provide its potential benefits at a reasonable cost, this paper presents a telepresence robot system for video communication which can deliver speaker's head motion through its display stanchion. Head gestures such as nodding and head-shaking can give crucial information during conversation. We also can assume a speaker's eye-gaze, which is known as one of the key non-verbal signals for interaction, from his/her head pose. In order to develop an efficient head tracking method, a 3D cylinder-like head model is employed and the Harris corner detector is combined with the Lucas-Kanade optical flow that is known to be suitable for extracting 3D motion information of the model. Especially, a skin color-based face detection algorithm is proposed to achieve robust performance upon variant directions while maintaining reasonable computational cost. The performance of the proposed head tracking algorithm is verified through the experiments using BU's standard data sets. A design of robot platform is also described as well as the design of supporting systems such as video transmission and robot control interfaces.