• Asian-Australasian Journal of Animal Sciences
• /
• 제32권3호
• /
• pp.350-356
• /
• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

• 대한물리치료과학회지
• /
• 제8권1호
• /
• pp.907-915
• /
• 2001

The purpose of this study is to effectively treat low back pain for the out patients who have been treated at the oriental hospital. Sorting the low back pain patients into four types based on scapular and ilium tilting hyper/hypo mobility characteristics, it has used several kinds of acupuncture stimuli on the 4 motor points of limb skeletal muscles, analyzing the acupuncture treatment on the 12 acupuncture points with which oriental hospitals has given to the low back pain patients and comparing with this effect. It has been analyzed how effectively the acupuncture stimuli has been contributed to the low back pain patients who suffered from skeletal muscles of spinal limb thorax abdominal and spinal skeletal system. The results are as follows. 1. There has been changes after treatment in both the control and experimental groups for low back pain patients, but the latter has been more increased in changes than the former. 2. There were much effects on the balanced restorations for spinal skeletal muscle in both the control group and experimental one, but on terms of restoration degree, the degree of the experimental group was higher than that of the control group. 3. Both the contrastive treatment and the experimental one have been contributed to the balanced restoration for all of the limb thorax abdominal muscles, but there were a few differences in terms of the way for restoration and the effects for improvement according to body types. 4. On both the control and the experimental group, spinal skeletal system has been restored with balance, but the experimental group has been higher effective on the balanced restoration than the one of the control group. Considering these results both the control and the experimental treatments have been contributed to the balanced restorations for all of low back pain patients who were suffering from skeletal muscles of spinal limb thorax abdominal and spinal skeletal system, but the contribution degree for the experimental group has been higher than that of the control group.

• 대한의용생체공학회:의공학회지
• /
• 제17권2호
• /
• pp.155-164
• /
• 1996

In the clinical environment, measurements of some characteristics of the skeletal muscle are currently used to assess the severity of a neuromuscular disease or in some cases to assist in making a diagnosis. But a quantitative method of evaluation has not yet been introduced satisfactorily. In this paper, the skeletal EMG(biceps muscle, masseter muscle) analysis has been processed both in the time and in the frequency domain by designing the digital signal processing system based on pentium PC and transputer (IMS 7805). The experiment have been performed in five normal subjects, and various parameters have been statistically tested and compare4 As a results, the effective parameters obtained for the evaluation of skeletal EMG electrical activity are turn analysis, MiTi, MiTa, IEMG, PDF in the time domain, and are mean frequency, median frequency, skewness, kurtosis, muscle fatigue slope in the frequency domain. The designed H/W and S/W in this study can be used effectively for the establishment of EMG data base and for clinical research.

• 대한치과의사협회지
• /
• 제40권1호통권392호
• /
• pp.68-74
• /
• 2002

• 대한추나의학회지
• /
• 제3권1호
• /
• pp.111-123
• /
• 2002

Objectives : There have been many studies of the effect of Bee Venom therapy about arthritis, but no one study was reported about its whole functional mechanism to musculo-skeletal system. This study was designed to investigate the effect, Indication, and side effect of Bee Venom therapy on musculo-skeletal disease by literature review of articles. Results : The effects of Bee Venom therapy to musculo-skeletal system are divided to Anti_inflammatory effect and Anti-nociceptive effect. Anti_inflammatory effect is achieved through competitive chemotaxis, immuno-regulation, increasing of cortisol secretion by stimulating hypothalamus-pituitary gland-adrenal cortex axis. Anti-nociceptive effect is achieved by Anti-inflammatory mechanism and it works similar to anti-nociceptive effect of the acupuncture acting on central and peripheral nociceptive transduction system. The Bee Venom therapy could cause severe side effect, for example, hypersensitivity and anaphylaxis, injury to central nerve system and cardiovascular system, peripheral blood system, and renal dysfunction. Conclusions : With its Anti-inflammatory and Anti-nociceptive mechanism, Bee Venom therapy is considered that has good effects to autoimmune disease, chronic inflammation of various musculo-skeletal disease and various pain syndrome. But the clinician must be careful for its side effects.

• Animal cells and systems
• /
• 제16권6호
• /
• pp.431-437
• /
• 2012

Calpains are a class of proteins that belong to the calcium-dependent, non-lysosomal cysteine proteases. There are three major types of calpains expressed in the skeletal muscle, namely, ${\mu}$-calpain, m-calpain, and calpain 3, which show proteolytic activities. Skeletal muscle fibers possess all three calpains, and they are $Ca^{2+}$-dependent proteases. The functional role of calpains was found to be associated with apoptosis and myogenesis. However, calpain 3 is likely to be involved in sarcomeric remodeling. A defect in the expression of calpain 3 leads to limb-girdle muscular dystrophy type 2A. Calpain 3 is found in skeletal muscle fibers at the N2A line of the large elastic protein, titin. A substantial proportion of calpain 3 is activated 24 h following a single bout of eccentric exercise. In vitro studies indicated that calpain 3 can be activated 2-4 fold higher than normal resting cytoplasmic [$Ca^{2+}$]. Characterization of the calpain system in the developing muscle is essential to explain which calpain isoforms are present and whether both ${\mu}$-calpain and m-calpain exist in differentiating myoblasts. Information from such studies is needed to clarify the role of the calpain system in skeletal muscle growth. It has been demonstrated that the activation of ubiquitous calpains and calpain 3 in skeletal muscle is very well regulated in the presence of huge and rapid changes in intracellular [$Ca^{2+}$].

• 대한통합의학회지
• /
• 제8권2호
• /
• pp.139-147
• /
• 2020

Purpose : Proprioceptive position sense plays a key role in providing joint stability, and multiple factors are related to proprioceptive position sense. Thus, this study aimed to determine the effects of body composition, particularly skeletal muscle mass on proprioceptive position sense following muscle fatigue. Methods : Healthy female subjects agreed to have their body composition analyzed. Only subjects who had 18.5-22.9 kg/㎡ of BMI (body mass index) were included in this study, and the participants were divided into two groups by skeletal muscle mass level. The experimental group had a level of skeletal muscle lower than the standard level (n=9), while the control group showed a standard or high level of skeletal muscle mass (n=11). To determine the change in proprioceptive position sense of the knee joint, the absolute angle error (AAE) was evaluated following muscle fatigue on low extremity. The muscle fatigue was induced by isokinetic resistance exercise program of Biodex system. AAE was measured by the Biodex system and compared the result before and after muscle fatigue. Results : The experimental group showed a significant AAE difference between before (3.16±2.48 °) and after (5.40±2.61 °) muscle fatigue. In addition, there was a AAE difference between the experimental (5.40±2.61 °) and control groups (3.53±1.67 °) after fatigue; however, there was no significance. Those results indicated that low level of skeletal muscle mass might influence the proprioceptive position sense of the knee joint after muscle fatigue. Conclusion : Thus, maintaining the proper level of skeletal muscle mass is pivotal to reduce the risk of injury following muscle fatigue in ADL or sport activities.

• Transactions on Control, Automation and Systems Engineering
• /
• 제4권1호
• /
• pp.43-48
• /
• 2002

We show that humans execute the postural control ingeniously by regulating the impedance properties of the musculo-skeletal system as the motor command against the alteration of the environment. Adjusting muscle activity can control the impedance properties of the musculo-skeletal system. To quantify the changes in human arm viscoelasticity on the vertical plane during interaction with the environment, we asked our subject to hold an object. By utilizing surface electromyographic(EMG) studies, we determined a relationship between the perturbation and a time-varying muscle co-activation. Our study showed when the subject lifts the object by himself the muscle stiffness increases while the torque remains the same just before the lift-off. These results suggest that the central nervous system(CNS) simultaneously controls not only the equilibrium point(EP) and the torque, but also the muscle stiffness as themotor command in posture control during the contact task.

• 한국치위생학회지
• /
• 제15권4호
• /
• pp.649-659
• /
• 2015

Objectives: The purpose of the study is to investigate the musculo-skeletal pain prevalence and severity in the dental hygienists based on PRECEDE model. Methods: A self-reported questionnaire was completed by 483 dental hygienists in Gwangju from September 13 to October 12, 2013. Data were analyzed by frequency analysis, chi-square test, t-test, and multiple logistic regression analysis using SPSS 18.0 program. Musculo-skeletal pain severity was classified from 1 to 5 by PRECEDE model. The questionnaire consisted of six questions of the general characteristics of the subjects, one question of musculo-skeletal pain prevalence, one question of body part musculo-skeletal pain prevalence, one question of subjective health status, three questions of activities of daily living, six questions of working environment, one question of musculoskeletal system diseases knowledge, two questions of social support, two questions of education experience and data use method, and five questions of necessity of health education. Results: The prevalence rate of musculo-skeletal pain within a year was 83.9% and 22.8% of the dental hygienists complained of severe pain. The odds ratio of moderate pain severity was 1.99(95% CI, 1.10-3.60) and the odds ratio of unhealthiness was 3.27 (95% CI, 1.35-7.94). The odds ratio of pain severity in those working for 4-6 years was 0.21(95% CI, 0.08-0.57). The odds ratio of pain severity in those practicing 6-10 scaling cases per day was 0.33(95% CI, 0.17-0.65). The odds ratio of pain severity in wrist turning and bending was 3.56(95% CI, 1.19-10.62). Conclusions: The muscolu-skeletal pain severity in the dental hygienists was closely associated with subjective health condition, work duration, the number of scaling practice activity, and a treatment posture. Regular physical checkup for the dental hygienists will improve the musculo-skeletal pain due to scaling practice.

• 대한치과교정학회지
• /
• 제34권5호
• /
• pp.458-464
• /
• 2004

교정치료에 있어서 고정원의 조절은 매우 중요한 요소이며, 상악에서 특히 그러하다. 이를 얻기 위해 많은 노력을 해 왔으나 대부분이 환자의 협조에 대한 의존이 필수적이었기 때문에 고정원 보강에 대하여 확신할 수 없었다. 하지만 최근 skeletal anchorage를 이용하여 환자의 협조를 최소화하면서도 보다 효과적으로 고정원 보강을 할 수 있는 방법이 시행되고 있다. 또한 과거의 골 융합성 임프란트와 달리 미니 스크류는 구강 내의 어느 부위에나 식립할 수 있을 정도로 식립 부위의 제한성이 적다는 장점이 있다. 저자는 titanium miniscrew를 구개 정중부의 약간 측방에 식립하고, indirect active P.S.A.(Palatal skeletal anchorage)를 이용하여 치료한 결과 상악 구치부에서의 고정원 보강을 얻을 수가 있었다. 이 치료 결과로 보아 PSA는 상악 구치부의 고정원 보강 역할을 할 수 있을 것으로 생각되며. 또한 transpalatal arch system의 다양한 design을 응용한다면 효율적인 치아 이동을 하는데 많은 도움이 될 것으로 생각된다.