• Title/Summary/Keyword: Skeletal system

Search Result 381, Processing Time 0.023 seconds

Effects of exercise on myokine gene expression in horse skeletal muscles

  • Lee, Hyo Gun;Choi, Jae-Young;Park, Jung-Woong;Park, Tae Sub;Song, Ki-Duk;Shin, Donghyun;Cho, Byung-Wook
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.32 no.3
    • /
    • pp.350-356
    • /
    • 2019
  • Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

The Experimental Study for the Balanced Restoration on the part of Spinal Skeletal Muscles in Low Back Pain Patients - By Acupuncture Stimuli Model of Limb Skeletal Muscle - (요통환자의 척주골격근 균형회복을 위한 실증적 연구 - 사지골격근의 침 자극모형을 중심으로 -)

  • Moon, Sang-Eun;Lee, Cheon-Bok
    • Journal of Korean Physical Therapy Science
    • /
    • v.8 no.1
    • /
    • pp.907-915
    • /
    • 2001
  • The purpose of this study is to effectively treat low back pain for the out patients who have been treated at the oriental hospital. Sorting the low back pain patients into four types based on scapular and ilium tilting hyper/hypo mobility characteristics, it has used several kinds of acupuncture stimuli on the 4 motor points of limb skeletal muscles, analyzing the acupuncture treatment on the 12 acupuncture points with which oriental hospitals has given to the low back pain patients and comparing with this effect. It has been analyzed how effectively the acupuncture stimuli has been contributed to the low back pain patients who suffered from skeletal muscles of spinal limb thorax abdominal and spinal skeletal system. The results are as follows. 1. There has been changes after treatment in both the control and experimental groups for low back pain patients, but the latter has been more increased in changes than the former. 2. There were much effects on the balanced restorations for spinal skeletal muscle in both the control group and experimental one, but on terms of restoration degree, the degree of the experimental group was higher than that of the control group. 3. Both the contrastive treatment and the experimental one have been contributed to the balanced restoration for all of the limb thorax abdominal muscles, but there were a few differences in terms of the way for restoration and the effects for improvement according to body types. 4. On both the control and the experimental group, spinal skeletal system has been restored with balance, but the experimental group has been higher effective on the balanced restoration than the one of the control group. Considering these results both the control and the experimental treatments have been contributed to the balanced restorations for all of low back pain patients who were suffering from skeletal muscles of spinal limb thorax abdominal and spinal skeletal system, but the contribution degree for the experimental group has been higher than that of the control group.

  • PDF

A Digital Signal Processing System for Analysis of Skeletal Muscle EMG Signal (골격근의 근전도 신호 분석을 위하 디지탈 신호처리 시스템의 설계)

  • 전철완
    • Journal of Biomedical Engineering Research
    • /
    • v.17 no.2
    • /
    • pp.155-164
    • /
    • 1996
  • In the clinical environment, measurements of some characteristics of the skeletal muscle are currently used to assess the severity of a neuromuscular disease or in some cases to assist in making a diagnosis. But a quantitative method of evaluation has not yet been introduced satisfactorily. In this paper, the skeletal EMG(biceps muscle, masseter muscle) analysis has been processed both in the time and in the frequency domain by designing the digital signal processing system based on pentium PC and transputer (IMS 7805). The experiment have been performed in five normal subjects, and various parameters have been statistically tested and compare4 As a results, the effective parameters obtained for the evaluation of skeletal EMG electrical activity are turn analysis, MiTi, MiTa, IEMG, PDF in the time domain, and are mean frequency, median frequency, skewness, kurtosis, muscle fatigue slope in the frequency domain. The designed H/W and S/W in this study can be used effectively for the establishment of EMG data base and for clinical research.

  • PDF

The Study of Literature Review on Mechanism of Bee Venom Therapy for Musculo-skeletal Disorder (봉독요법(蜂毒療法)의 근골격계질환(筋骨格界疾患) 치료기전(治療機轉)에 대한 문헌적(文獻的) 고찰(考察))

  • Kim, Sung-Soo;Chung, Won-Suk
    • The Journal of Korea CHUNA Manual Medicine
    • /
    • v.3 no.1
    • /
    • pp.111-123
    • /
    • 2002
  • Objectives : There have been many studies of the effect of Bee Venom therapy about arthritis, but no one study was reported about its whole functional mechanism to musculo-skeletal system. This study was designed to investigate the effect, Indication, and side effect of Bee Venom therapy on musculo-skeletal disease by literature review of articles. Results : The effects of Bee Venom therapy to musculo-skeletal system are divided to Anti_inflammatory effect and Anti-nociceptive effect. Anti_inflammatory effect is achieved through competitive chemotaxis, immuno-regulation, increasing of cortisol secretion by stimulating hypothalamus-pituitary gland-adrenal cortex axis. Anti-nociceptive effect is achieved by Anti-inflammatory mechanism and it works similar to anti-nociceptive effect of the acupuncture acting on central and peripheral nociceptive transduction system. The Bee Venom therapy could cause severe side effect, for example, hypersensitivity and anaphylaxis, injury to central nerve system and cardiovascular system, peripheral blood system, and renal dysfunction. Conclusions : With its Anti-inflammatory and Anti-nociceptive mechanism, Bee Venom therapy is considered that has good effects to autoimmune disease, chronic inflammation of various musculo-skeletal disease and various pain syndrome. But the clinician must be careful for its side effects.

  • PDF

The role of calpain in skeletal muscle

  • Pandurangan, Muthuraman;Hwang, Inho
    • Animal cells and systems
    • /
    • v.16 no.6
    • /
    • pp.431-437
    • /
    • 2012
  • Calpains are a class of proteins that belong to the calcium-dependent, non-lysosomal cysteine proteases. There are three major types of calpains expressed in the skeletal muscle, namely, ${\mu}$-calpain, m-calpain, and calpain 3, which show proteolytic activities. Skeletal muscle fibers possess all three calpains, and they are $Ca^{2+}$-dependent proteases. The functional role of calpains was found to be associated with apoptosis and myogenesis. However, calpain 3 is likely to be involved in sarcomeric remodeling. A defect in the expression of calpain 3 leads to limb-girdle muscular dystrophy type 2A. Calpain 3 is found in skeletal muscle fibers at the N2A line of the large elastic protein, titin. A substantial proportion of calpain 3 is activated 24 h following a single bout of eccentric exercise. In vitro studies indicated that calpain 3 can be activated 2-4 fold higher than normal resting cytoplasmic [$Ca^{2+}$]. Characterization of the calpain system in the developing muscle is essential to explain which calpain isoforms are present and whether both ${\mu}$-calpain and m-calpain exist in differentiating myoblasts. Information from such studies is needed to clarify the role of the calpain system in skeletal muscle growth. It has been demonstrated that the activation of ubiquitous calpains and calpain 3 in skeletal muscle is very well regulated in the presence of huge and rapid changes in intracellular [$Ca^{2+}$].

The Effects of Skeletal Muscle Mass and Muscle Fatigue on the Proprioceptive Position Sense of the Knee Joint (뼈대근육량과 근피로가 무릎관절 고유수용성 위치감각에 미치는 영향)

  • Park, Sookyoung;Park, Kanghui
    • Journal of The Korean Society of Integrative Medicine
    • /
    • v.8 no.2
    • /
    • pp.139-147
    • /
    • 2020
  • Purpose : Proprioceptive position sense plays a key role in providing joint stability, and multiple factors are related to proprioceptive position sense. Thus, this study aimed to determine the effects of body composition, particularly skeletal muscle mass on proprioceptive position sense following muscle fatigue. Methods : Healthy female subjects agreed to have their body composition analyzed. Only subjects who had 18.5-22.9 kg/㎡ of BMI (body mass index) were included in this study, and the participants were divided into two groups by skeletal muscle mass level. The experimental group had a level of skeletal muscle lower than the standard level (n=9), while the control group showed a standard or high level of skeletal muscle mass (n=11). To determine the change in proprioceptive position sense of the knee joint, the absolute angle error (AAE) was evaluated following muscle fatigue on low extremity. The muscle fatigue was induced by isokinetic resistance exercise program of Biodex system. AAE was measured by the Biodex system and compared the result before and after muscle fatigue. Results : The experimental group showed a significant AAE difference between before (3.16±2.48 °) and after (5.40±2.61 °) muscle fatigue. In addition, there was a AAE difference between the experimental (5.40±2.61 °) and control groups (3.53±1.67 °) after fatigue; however, there was no significance. Those results indicated that low level of skeletal muscle mass might influence the proprioceptive position sense of the knee joint after muscle fatigue. Conclusion : Thus, maintaining the proper level of skeletal muscle mass is pivotal to reduce the risk of injury following muscle fatigue in ADL or sport activities.

Human Arm Posture Control Using the Impedance Controllability of the Musculo-Skeletal System Against the Alteration of the Environments

  • Kim, Jaehyo;Makoto Sato;Yasuharu Koike
    • Transactions on Control, Automation and Systems Engineering
    • /
    • v.4 no.1
    • /
    • pp.43-48
    • /
    • 2002
  • We show that humans execute the postural control ingeniously by regulating the impedance properties of the musculo-skeletal system as the motor command against the alteration of the environment. Adjusting muscle activity can control the impedance properties of the musculo-skeletal system. To quantify the changes in human arm viscoelasticity on the vertical plane during interaction with the environment, we asked our subject to hold an object. By utilizing surface electromyographic(EMG) studies, we determined a relationship between the perturbation and a time-varying muscle co-activation. Our study showed when the subject lifts the object by himself the muscle stiffness increases while the torque remains the same just before the lift-off. These results suggest that the central nervous system(CNS) simultaneously controls not only the equilibrium point(EP) and the torque, but also the muscle stiffness as themotor command in posture control during the contact task.

The related factors of severity of musculo-skeletal pain in the dental hygienists based on PRECEDE model (PRECEDE 모형을 적용한 치과위생사의 근골격계 통증 심각도 관련 요인)

  • Moon, Ae-Eun;Park, Jong
    • Journal of Korean society of Dental Hygiene
    • /
    • v.15 no.4
    • /
    • pp.649-659
    • /
    • 2015
  • Objectives: The purpose of the study is to investigate the musculo-skeletal pain prevalence and severity in the dental hygienists based on PRECEDE model. Methods: A self-reported questionnaire was completed by 483 dental hygienists in Gwangju from September 13 to October 12, 2013. Data were analyzed by frequency analysis, chi-square test, t-test, and multiple logistic regression analysis using SPSS 18.0 program. Musculo-skeletal pain severity was classified from 1 to 5 by PRECEDE model. The questionnaire consisted of six questions of the general characteristics of the subjects, one question of musculo-skeletal pain prevalence, one question of body part musculo-skeletal pain prevalence, one question of subjective health status, three questions of activities of daily living, six questions of working environment, one question of musculoskeletal system diseases knowledge, two questions of social support, two questions of education experience and data use method, and five questions of necessity of health education. Results: The prevalence rate of musculo-skeletal pain within a year was 83.9% and 22.8% of the dental hygienists complained of severe pain. The odds ratio of moderate pain severity was 1.99(95% CI, 1.10-3.60) and the odds ratio of unhealthiness was 3.27 (95% CI, 1.35-7.94). The odds ratio of pain severity in those working for 4-6 years was 0.21(95% CI, 0.08-0.57). The odds ratio of pain severity in those practicing 6-10 scaling cases per day was 0.33(95% CI, 0.17-0.65). The odds ratio of pain severity in wrist turning and bending was 3.56(95% CI, 1.19-10.62). Conclusions: The muscolu-skeletal pain severity in the dental hygienists was closely associated with subjective health condition, work duration, the number of scaling practice activity, and a treatment posture. Regular physical checkup for the dental hygienists will improve the musculo-skeletal pain due to scaling practice.

Indirect palatal skeletal anchorage (PSA) for treatment of skeletal Class I bialveolar protrusion (Indirect palatal skeletal anchorage (PSA)를 이용한 골격성 I급 양악 치성 전돌 환자의 치험례)

  • Chae, Jong-Moon
    • The korean journal of orthodontics
    • /
    • v.34 no.5 s.106
    • /
    • pp.458-464
    • /
    • 2004
  • Anchorage plays an important role in orthodontic treatment especially in the maxillary arch. In spite of many efforts for anchorage control. it was difficult for clinicians to predict the result of treatment because most of the treatment necessitated an absolute compliance of patients, But recently, skeletal anchorage has been used widely because it does not necessitate patient compliance but produces absolute anchorage. In addition titanium miniscrews have several advantages such as ease of insertion and removal. possible immediate leading and use in limited implantation spaces. In this case, a skeletal Class I bialveolar protrusion Patient was treated with standard edgewise mechanics using indirect active P.S.A. (palatal skeletal anchorage). The miniscrews in the paramedian area of the hard palate provided anchorage for retraction of the upper anterior teeth and remained firm and stable throughout treatment This indicates that the PSA can be used to reinforce anchorage for orthodontic treatment in the maxillary arch Consequently, this new approach can help effective tooth movement without patient compliance, when used with various transpalatal arch systems.