• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.4
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• pp.81-97
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• 2019

The purpose of this study is to examine what people in Korea recognize the cultural symbolism and the planting patterns of 'Guǐmok', pagoda tree(Sophora japonica). The species planted in the 'Guǐjeong' was empirically investigated and analyzed to determine which species of pagoda tree or Zelkova tree(Zelkova serrata) was taken through literature surveys, field surveys, and interviews with persons. This 'Guǐjeong' was combined to track how the culture of the 'Guǐmok' planting introduced in China was ultimately accepted and transformed in Korea. In this study, we tried to analyze the meaning implicit in the mystery while checking the distribution of the mystery and the form of the mystery, the name of the pavilion and its relevance to the contrast medium. Essentially, the trees that govern the characterization of the nectar plant, regardless of the region, are considered to be a pagoda tree, which is considered an internal factor in which the pagoda tree culture was not completely transformed into a zelkova tree. It was recognized throughout the Joseon Dynasty that the species representing 'Prime ministers(三公)' was judged from all the Joseon Dynasty periods, based on the builder of Guǐjeong's Aho(雅號) and Dangho(堂號). It was confirmed that the tree was very likely to be planted in place of the painting tree. But now is selectively zelkova tree is in accordance with the preparation of planting site conditions and areas on behalf of the Change is very high probability that is planted. Cultural variables that led to the cultural transformation of the 'Guǐmok' seem to have been deeply involved in the geographical space of China and Korea, Confucian practices of the Choson society, comings and goings and letter bridge, and network strength with China through the book spread. In addition, the culture of 'Guǐmok' is presumed to have led to cultural custom of the upper class, not the whole class, in the Yeongnam region, it can be said that the independent adaptation to act to recognize 'Guǐmok' as a pagoda tree, that is a Sophora japonica, has occurred very strongly. The difference between the cultural areas of Yeongnam and non-Yeongnam is also considered to be an internal factor that has played a major role in the cultural transformation of planting of 'Guǐmok'.

• The Journal of Engineering Geology
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• v.28 no.2
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• pp.133-160
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• 2018

$CO_2$ storage is a very important technology for reduction of greenhouse gas emissions and has been considered as almost the only viable and effective option for immediate large-scale $CO_2$ sequestration. Small-scale demonstration project for offshore $CO_2$ storage in the Pohang Basin is the transitional stage R&D program for technological preparation of large-scale $CO_2$ storage project in Korea. Through the extensive exploration research for prospective $CO_2$ storage sites, the offshore strata in the Pohang Basin was recommended for the storage formation of the small-scale demonstration project. The Pohang Offshore Storage Project launched at 2013, and has accomplished the technical demonstration and technological independence in a wide range of $CO_2$ storage technology, such as geophysical exploration, storage site characterization, storage design, offshore platform construction, injection-well drilling and completion, deployment of injection facility, operation of $CO_2$ injection, and $CO_2$ monitoring. The project successfully carried out $CO_2$ test injection in early 2017, and achieved its final goal for technical development and demonstration of $CO_2$ storage in Korea. The realization of $CO_2$ injection in this project is the measurable result and has been recorded as the first success in Korea. The Pohang Offshore Storage Project has a future plan for the continuous operation of $CO_2$ injection and completion of $CO_2$ monitoring system. The project has provided in-house technical and practical expertises, which will be a solid foundation for the commercial-scale $CO_2$ storage business in Korea. Additionally, the project will help to secure national technical competitiveness in growing international technology market for $CO_2$ storage.

• The Korean Journal of Pharmacology
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• v.30 no.1
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• pp.125-136
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• 1994

To characterize the SR Ca-release channel protein complex of crustacean, $^{45}Ca-release,\;[^3H]ryanodine$ binding, and immunoblot studies were carried out in the crayfish and/or lobster skeletal sarcoplasmic reticulum. Bmax and affinity of crayfish SR to ryanodine were lower than those of lobster SR. AMP (5mM) increased $[^3H]ryanodine$ binding significantly in both vesicles (P<0.05). $Mg^{2+}$(5mM) or tetracaine(1mM) inhibited $[^3H]ryanodine$ binding significantly in both vesicles (P<0.001), but ruthenium red $(10\;{\mu}M)$ inhibited it moderately. In SDS polyacrylamide gel electrophoretic analysis of crayfish SR vesicles, there was a high molecular weight band that showed similar mobility with Ca-release channel protein of lobster skeletal SR, but more rapid mobility (HMWBr) than that of rabbit skeletal SR (HMWBS). Immunoblot analysis showed that polyclonal Ab to lobster skeletal SR Ca-release channel protein was react with HMWBr of crayfish skeletal SR, but not with that of HMWBs of rabbit skeletal SR. ^{45}Ca-release from crayfish skeletal SR vesicles was increased by the increase of extravesicular calcium from $1{\mu}M$ to 1mM. This Ca-release phenomenon was similar, but more sensitive in the low concentration of $Ca^{2+}$, compared to that from lobster SR vesicles. AMP (5mM) or caffeine (10mM) did not affect to $^{45}Ca-release.\;^{45}Ca-release$ was inhibited slightly ($3{\sim}8%\;by\;Mg^{2+}$) (5mM) or tetracaine (1mM), and moderately (23%) by high concentration of ruthenium red $(300\;{\mu}M)$. From the above results, it is suggested that SR Ca-release channel protein of crustacean has different properties from that of the rabbit, and similar properties between crayfish and lobster in functional and immunological aspects, but Ca-release via crayfish channel may be more sensitive to calcium.

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.183-190
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• 2009

Characterization of the analysis of forest vegetation, soil environmental conditions and water quality were performed from March 2003 to March 2007. The two basins were characterized by cultivated area (Kaesim reservoir) and mountain area (Jangchan reservoir), and divided into eleven small basins, where dynamics of pollutants, forest vegetation and soil environmental conditions were surveyed. The vegetation can be divided into 10 types by $Z\ddot{u}rich$-Montpellier school's method. Pearson coefficients between vegetation type and water quality were correlated with dissolved oxygen (DO) in the Quercus variabilis community at the 5% level and total phosphorus (TP) in the Larix leptolepis plantation at the 1% level. Especially total phosphorous and total nitrogen increased in small basins where the proportion of cultivated and residential area increased. The analysis of influences of pollutant discharge on water quality showed that pollutant charge was very low in forest land area ($Y_{T-P}$=-0.0017X+0.2215, r=0.16, $Y_{COD}$=- 0.0395X+8.5051 r=0.47). The soil types of western area were comparatively simple, but those of eastern area were complicated with regosols, red-yellow soils, lithosoles, etc. The pH, total solid (TS) and volatile substance (VS) of the forest and agricultural land soils collected in each site were 5.4~6.9, 75.8~80.2%, and 3.80%~5.80%, respectively. According to the analytical result of soil environmental conditions, heavy metal contents fell short to the mean value of natural conditions. Runoff amount (Y) and depth of topsoil (X) were negatively correlated, $Y_{ron}=-1.0088X_{top}+35.378$ (r=0.68). The correlation was much lower in up-stream but much higher in down-stream, because permeation into soil particle was larger on down-stream due to its more or less gentle slope. Pearson coefficients between soil pH and water pH were statistically significant at 1% level.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.167-173
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• 2007

To study the transcriptional mechanisms by which expression of the murine glial cell-derived neurotrophic factor inducible transcription factor (mGIF) gene is regulated, a murine genomic clone was iso-lated using a mGIF cDNA as probe. A 13-kb genomic fragment, which comprises 4-kb upstream of the transcription initiation site was sequenced. The promoter region lacks a TATA box and CAAT box, is rich in G+C content, and has multiple putative binding sites for the transcription factor Spl. The mGIF gene also has consensus sequences for AP2 binding sites. The transcriptional activity of five deletion mutants of a 2.1-kb fragment was analyzed by modulating transcription of the heterologous luciferase gene in the promoterless plasmid pGL2-Basic. All mutants showed significant transcriptional activity in the murine neuroblastoma cell line NB41A3. Transient expression assays suggested the presence of a positive regulator between -213 and -129 while a negative regulator was found in the region between -806 and -214. Relatively strong transcriptional activity was observed in neuronal NB41A3, glial C6 cells and hepatic HepG2, but very weak activity in skeletal muscle C2C12 cells. These findings confirm the tissue-specific activity of the mGIF promoter and suggest that this gene shares structural and functional similarities with the dopamine receptor genes that it regulates.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1133-1140
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• 2006

Human HtrA1 (High temperature requirement protein A1) is a homologue of the E. coli periplasmic serine protease HtrA. A recent study has demonstrated that HtrA1 is a serine protease involved in processing of insulin like growth factor binding protein (ICFBP), indicating that it serves as an important regulator of IGF activity. Additionally, several lines of evidence suggest a striking correlation between proteolytic activity of HtrA1 serine protease and the pathogenesis of several diseases; however, physiological roles of HtrA1 remain to be elucidated. We used the pGEX bacterial expression system to develop a simple and rapid method for purifying HtrA1, and the recombinant HtrA1 protein was utilized to investigate the optimal conditions in executing its proteolytic activity. The proteolytically active HtrA1 was purified to approximately 85% purity, although the yield of the recombinant HtrA1 protein was slightly low $460{\mu}g$ for 1 liter E. coli culture). Using in vitro endoproteolytic cleavage assay, we identified that the HtrA1 serine protease activity was dependent on the enzyme concentration and the incubation time and that the best reaction temperature was $42^{\circ}C$ instead of $37^{\circ}C$. We arbitrary defined one unit of proteolytic activity of the HtrA1 serine protease as 200nM of HtrA1 that cleaves half of $5{\mu}M\;of\;{\beta}-casein$ during 3 hr incubation at $37^{\circ}C$. Our study provides a method for generating useful reagents to investigate the molecular mechanisms by which HtrA1 serine protease activity contributes in regulating its physiological function and to identify natural substrates of HtrA1.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.11
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• pp.1213-1221
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• 2006

In this study, granular sludge used in an anaerobic process treating brewery waste was inoculated in a laboratory scale of reactor to induce anaerobic ammonium oxidation(ANAMMOX). The reactor was operated with synthetic wastewater, which prepared at 1:1 ratio of $NH_4^+-N$ over $NO_2^--N$. Changes in nitrogen concentration, COD, alkalinity and gas production were analyzed. There are 3 phases of spanning in experimental period according to influent nitrogen concentration. In the Phase 1, each of the concentration of $NH_4^+-N$ and $NO_2^--N$ were increased from 1.91 $gN/m^3{\cdot}d$ to 14.29 $gN/m^3{\cdot}d$. Ammonium nitrogen loading(same as nitrite nitrogen) was 23.81 $gN/m^3{\cdot}d$ in the Phase 2 and 19.05 $gN/m^3{\cdot}d$ in the Phase 3, respectively $NO_2^--N$ has been removed up to 99% during whole period while the removal efficiency of $NH_4^+-N$ was significantly varied. In Phase 2, $NH_4^+-N$ was removed up to 75%. Microorganisms varied temporally through three phases were characterized by 16s rDNA analysis methods. ANAMMOX bacteria were dominantly found in phase 2 when the removal rate of $NO_2^--N$and $NH_4^+-N$ was the highest up to 99% and 75%, respectively. Due to erroneous exposed to air, the removal efficiency of $NH_4^+-N$ was unexpectedly lowered, but ANAMMOX bacteria still existed.

• The Journal of Engineering Geology
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• v.28 no.2
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• pp.217-246
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• 2018

During the selection and characterization of target formations in the Small-scale Offshore $CO_2$ Storage Demonstration Project in the Pohang Basin, we have carefully investigated the possibility of induced earthquakes and leakage of $CO_2$ during the injection, and have designed the storage processes to minimize these effects. However, people in Pohang city have a great concern on $CO_2$-injection-intrigued seismicity, since they have greatly suffered from the 5.4 magnitude earthquake on Nov. 15, 2017. The research team of the project performed an extensive self-investigation on the safety issues, especially on the possible $CO_2$ leakage from the target formation and induced earthquakes. The target formation is 10 km apart from the epicenter of the Pohang earthquake and the depth is also quite shallow, only 750 to 800 m from the sea bottom. The project performed a pilot injection in the target formation from Jan. 12 to Mar. 12, 2017, which implies that there are no direct correlation of the Pohang earthquake on Nov. 15, 2017. In addition, the $CO_2$ injection of the storage project does not fracture rock formations, instead, the supercritical $CO_2$ fluid replaces formation water in the pore space gradually. The self-investigation results show that there is almost no chance for the injection to induce significant earthquakes unless injection lasts for a very long time to build a very high pore pressure, which can be easily monitored. The amount of injected $CO_2$ in the project was around 100 metric-tonne that is irrelevant to the Pohang earthquake. The investigation result on long-term safety also shows that the induced earthquakes or the reactivation of existing faults can be prevented successfully when the injection pressure is controlled not to demage cap-rock formation nor exceed Coulomb stresses of existing faults. The project has been performing extensive studies on critical stress for fracturing neighboring formations, reactivation stress of existing faults, well-completion processes to minimize possible leakage, transport/leakage monitoring of injected $CO_2$, and operation procedures for ensuring the storage safety. These extensive studies showed that there will be little chance in $CO_2$ leakage that affects human life. In conclusion, the Small-scale Offshore $CO_2$ Storage Demonstration Project in the Pohang Basin would not cause any induced earthquakes nor signifiant $CO_2$ leakage that people can sense. The research team will give every effort to secure the safety of the storage site.

• Economic and Environmental Geology
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• v.40 no.2 s.183
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• pp.191-207
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• 2007

A paleomagnetic investigation for the Cretaceous rocks in the Buyeo and Hampyeong Basins, located out of the Gyeongsang Basin, was carried out in order to elucidate the paleomagnetic directions in conjunction with the formation of the basins. Typical stepwise thermal demagnetization and measurement methods were used to determine the directions of characteristic remanent magnetizations (ChRMs). The mean direction of the sedimentary rocks from the Buyeo Basin after bedding correction $(D/I=356.5^{\circ}/61.5^{\circ},\;k=39.3\;\alpha_{95}=7.4^{\circ})$, is more dispersed than that before bedding correction $(D/I=356.5^{\circ}/61.5^{\circ},\;k=39.3\;\alpha_{95}=7.4^{\circ})$, which suggests that the rocks in the Buyeo Basin were remagnetized. However, the statistics and dispersion of the ChRM directions after bedding correction are still acceptable and the paleomagnetic pole position after tilt correction $(Lat./Long.=69.3^{\circ}N/186.7^{\circ}E,\;K=11.6\;A_{95}=14.0^{\circ})$ is closer to that of the Late Cretaceous pole of the Korean Peninsula. More detailed study is needed to confirm the nature of the remagnetization in the Buyeo Basin. On the other hand, the paleomagnetic pole before bedding correction $(Lat./Long.=81.6^{\circ}N/106.9^{\circ}E,\;K=25.1\;A_{95}=9.3^{\circ})$ is positioned near the paleogene pole of the Eurasian APWP. The mean ChRM direction of the sedimentary rocks from the Hampyeong Basin after bedding correction is $D/I=32.5^{\circ}/55.4^{\circ},\;(k=35.6,\;\alpha_{95}=8.7^{\circ})$. It is more clustered than that before bedding correction $D/I=18.3^{\circ}/62.5^{\circ},\;k=14.1,\;\alpha_{95}=14.2^{\circ})$, indicating that the ChRM was acquired before tilting of the strata. The paleomagnetic pole position of the Cretaceous sedimentary rocks in the Hampyeong Basin, averaged out of site pole positions calculated from the tilt-corrected ChRMs, is $Lat./Long.=63.9^{\circ}N/202.7^{\circ}E,\;(K=21.3,\;A_{95}=7.6^{\circ})$, similar to the Late Cretaceous paleomagnetic pole of the Korean Peninsula $(Lat./Long.=70.9^{\circ}N/215.4^{\circ}E,\;A_{95}=5.3^{\circ})$, suggesting that the Hampyeong Basin has been stable since the Late Cretaceous period. One normal and two reversed ChRM directions are revealed through the measurements of the volcanic rocks from the Hampyeong Basin. Although these normal and reversed directions are not exactly antipodal, it is interpreted that the normal direction is the representative primary direction of the volcanic rocks of the Hampyeong Basin and the mixed polarity is the records of geomagnetic field at the time of the formation of the volcanic rocks. Paleomagnetic poles are at $Lat./Long.=70.2^{\circ}N/199.5^{\circ}E,\;(K=18.1,\;A_{95}=9.6^{\circ})$ for the normal direction, and $Lat./Long.=65.5^{\circ}S/251.3^{\circ}E,\;(K=7.1,\;A_{95}=20.7^{\circ})$ for the reversed direction. Compared with the representative pole positions of the Cretaceous period of the Korean Peninsula, it is concluded that the age of the volcanic rocks in the Hampyeong Basin is of the Late Cretaceous.