• 화약ㆍ발파
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• 제16권3호
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• pp.25-34
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• 1998

This area is covered in Andesite of high compression strength and located in PUSAN SEO-KU. There are many old houses around shaft site. So, we must have a cautious blasting operation. A total of 40 blasts were test at DAE-SHIN Shaft site to study the magnitude and frequency characterization of blast-induced vibration. The effect of viblating frequency on structual damage and site-specific scaling to define th empirical equations were also discussed. The result can be summarized as follows: 1. Some empirical equations were obtained. $V=K\{{\frac{D}{W}}1/3\}^{-n}$ where the values for n and K are estimated to be -1.407 to -2.202 and 643.3489 to 7283.2104 respectively. 2. Dominant frequencies at short distance are in the range of about 75.0 to 91.8 Hz, with some exceptions of about 50Hz, Frequencies observed at long distance are in the range of 10 to 2Hz. It is apparent the shift of dominant frequency down to lower levels at long distance.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.173-177
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• 1997

Glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents diethylpyrocarbonate, N-bromosuccinimide, or carbodiimide resulted in complete loss of enzyme activity, which shows histidine, tryptophan, and glutamic acid or aspartic acid residues are at or near the active site. In each case, inactivation followed pseudo first-order kinetics. Inclusion of glycerol-3-phosphate and/or CTP prevented the inactivation, indicating the presence of tryptophan and glutamic acid or aspartic acid residues at the substrate binding site. Analysis of kinetics of inactivation showed that the loss of enzyme activity was due to modification of a two histidine residues, single tryptophan residue, and two glutamic acid or aspartic acid residues.

• Archives of Pharmacal Research
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• 제14권3호
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• pp.231-236
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• 1991

The high affinity binding sites for angiotensin II were solubilized from rat liver membranes by treatment with CHAPS. The binding protein was also partially purified by angiotensin III inhibitor-coupled Affi-gel affinity chromatography. Binding to the intact membrances as well as to the solubilized preparation was specific and saturable. According to the Scatchard plot, the membrane preparations exhibited a single class of high affinity binding sites with a Kd OF 0.71 nM. The solubilized preparation also showed the presence of a single class of bindings sites with less affinity (Kd of 14 nM). Meanwhile the competition studies using angiotensin II analogues represented two separate binding sites for angiotensin II and single binding site for antagonist. These latter findings were correlated to the results provided by Garrison's research group. More works are needed to clarify this discrepancy.

• Wind and Structures
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• 제31권4호
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• pp.311-319
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• 2020

India is a developing nation and heavily spends on the development of wind power plants to meet the national energy demand. The objective of this paper is to investigate wind power potential of Ennore site using wind data collected over a period of two years by three parameter Weibull distribution. The Weibull parameters are estimated using maximum likelihood, least square method and moment method and the accuracy is determined using R² and root mean square error values. The site specific capacity factor is calculated by the mathematical model developed by three parameter Weibull distribution at different hub heights above the ground level. At last, the wind energy economic analysis is carried out using capacity factor at 30 m, 40 m and 50 m height for different wind turbine models. The analysis showed that the site has potential to install utility wind turbines to generate energy at the lowest cost per kilowatt-hour at height of 50 m. This research provides information of wind characteristics of potential sites and helps in selecting suitable wind turbine.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.55-55
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• 1993

Farnesyl Protein transferase(FPT)는 발암유전자 ras의 단백질 산물인 p$^{21}$의 post-translational modification의 첫 단계인 ras-farnesylation에 관여하는 효소로 본 연구에서는 정제된 FPT와 E. coli에서의 발현 system을 이용하여 FPT의 구조와 기능을 밝히고 이를 FPT 방해제의 설계에 이용하고자 한다. Bovine testis에 존재하는 FPT를 30%-50%의 Ammonium sulfate로 fractionation하고, DEAE-Sephacel, Sephacryl S-300 column을 통과시킨 후 peptide(KKCVIM) affinity column을 이용하여 순수 정제하였다. 정제된 효소의 분자량은 gel-filtration에 의해 100KDa으로 추정되었고 SDS-PAGE 결과 49KDa과 46KDa의 두 subunit로 구성되었음이 확인되었다. 효소활성에는 $Mg^{2＋}$ 와 $Zn^{2＋}$가 필수적이며 최적 pH는 7.0이었다. Yeast의 FPT의 두 subunit 유전자는 Yeast genomic DNA를 template로 사용하고 각 subunit에 specific한 합성된 primer들과 vent polymerase를 이용하여 Polymerase chain reaction을 통하여 얻었다. 두 유전자를 pBluescriptII SK＋ vector를 변형시킨 두 vector, pBSK＋4와 pBChl＋4에 재조합 시킨 후 E.coli에 transformation시켜 발현시켰다. 현재 정제된 Bovine FPT와 E. coli에서 발현된 Yeast FPT의 chemical modification과 site-directed mutagenesis를 통하여 FPT의 active site와 substrate binding site에 관한 연구를 진행시키고 있다.

• Archives of Pharmacal Research
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• 제20권2호
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• pp.115-121
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• 1997

Ile-269 in horse liver alcohol dehydrogenase isoenzyme S(HLADH-S) was mutated to serine by phosphorothioate-based site-directed mutagenesis in order to study the role of the residue in coenzyme binding. The specific activity of the mutant(1269S) enzyme to ethanol was increased 49-fold. All turnover numbers of 1269S enzyme toward 9 primary alcohols were increased. The mutant enzyme showed 3.6, 4.6, 11.6-fold higher catalytic efficiency for $5{\beta}$-androstane-3, 17-dione, $5{\beta}$-cholanic acid-3-one and retinal than wild-type, respectively. The reaction mechanism of 1269S enzyme was ordered bi bi as wild-type's. These results indicate that the hydrophobic interaction of Ile-269 residue with coenzyme plays an important role in dissociation of coenzyme from enzyme-coenzyme complex, which has been known as the rate limiting step of ADH reaction.

• Molecules and Cells
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• 제39권1호
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• pp.53-59
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• 2016

Peroxiredoxins are cysteine-dependent peroxide reductases that group into 6 different, structurally discernable classes. In 2011, our research team reported the application of a bioinformatic approach called active site profiling to extract active site-proximal sequence segments from the 29 distinct, structurally-characterized peroxiredoxins available at the time. These extracted sequences were then used to create unique profiles for the six groups which were subsequently used to search GenBank(nr), allowing identification of ~3500 peroxiredoxin sequences and their respective subgroups. Summarized in this minireview are the features and phylogenetic distributions of each of these peroxiredoxin subgroups; an example is also provided illustrating the use of the web accessible, searchable database known as PREX to identify subfamily-specific peroxiredoxin sequences for the organism Vitis vinifera (grape).

• 한국토양비료학회지
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• 제45권1호
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• pp.9-15
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• 2012

This study was performed to produce basic data for silty reclaimed tidal lands and to develop techniques of environmentally-friendly utilization in agricultural system. We chose the two sites in Saemangeum reclaimed tidal lands, one (Site I) has been treated with cultivating green manure and conducting the desalinization process through submergence since April, 2007 and the other (Site II) has been under natural condition without artificial treatment. In situ and ex situ physic-chemical properties were determined and comparisons were made for soil profiles examined at these two sites in April 2009. Surface soil of Site I had lower EC and higher field saturated hydraulic conductivity than those of Site II, uncultivated land. Especially, exchangeable sodium content was lowest in Site I Ap1 layer than in other layers. This is probably due to flooding desalination and green manure cultivation. Besides, Ap1 and A2 layers of soil profile in Site I showed brighter soil color and more root observation than those of Site II. This is probably due to green manure cultivation. By the large, for high cash upland crops and intensive agricultural use of silty reclaimed tidal land, site-specific soil ripening such as flooding desalination and green manure cultivation could be useful.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.35-39
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• 2001

The Integration Host factor (IHF) of Escherichia coli is a small, basic protein that is required for a variety of functions including site-specific recombination, transposition, gene regulation, plasmid replication, and DNA packaging. It ,is composed of two subunits that are encoded by the ihfA ($\alpha$-subunit) and ihjB ($\beta$-subunit) genes. IHF binding sites are composed of three elements called the WATCAR, TTG, and poly (dAT) elements. We have characterized IHF binding to the H site of bacteriophage λ. We have isolated suppressors that bind to altered H' sites using a challenge phage selection. Two different suppressors were isolated that changed the adjacent $\alpha$P64 and $\alpha$K65 residues. The suppressors recognized both the wild-type site and a site with a change in the WATCAR element. Three suppressors were isolated at $\beta$-E44. These suppressors bound the wild-type and a mutant site with a T：A to A：T change (H44A) in the middle of the TIR element. Site-directed mutagenesis was used to make several additional changes at $\beta$E44. The wild-type and $\beta$E44D mutant could not bind the wild-type site but were able to bind the H44A mutant site. Other mutants with neutral, polar, or a positive charge at $\beta$E44 were able to repress both the wild-type and H44A sites. Examination of the IHF crystal structure suggests that the ability of the wild-type and $\beta$E44D proteins to discriminate between the T：A and A：T basepairs is due to indirect interactions. The $\beta$-E44 residue does not contact the DNA directly. It imposes binding specificity indirectly by interactions with residues that contact the DNA. Details of the proposed interactions are discussed.

• Journal of Forest and Environmental Science
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• 제39권1호
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• pp.27-39
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• 2023

Twenty-five Eucalyptus clones (14 E. camaldulensis - EC and 11 interspecific eucalypt hybrid clones - EH) grown in three contrasting sites were evaluated for the growth and few wood traits at 4 years of age. The stability, genotype-site interaction and suitability of these clones for pulp and solid wood industry sectors were studied. Growth of eucalypt clones was significantly higher at site 1 with higher rainfall, but wood density did not differ significantly from lower rainfall sites. Kraft pulp yield (KPY) decreased from sites 1 to 3 based on moisture availability, but not between two groups of clones. Volumetric shrinkage (VS) was significantly higher in EC clones at site 3 with lowest rainfall, but there was no specific trend at other two sites with maximum (site 1) and intermediate (site 2) rainfall. The mechanical traits modulus of rupture (MOR) and modulus of elasticity (MOE) were at par in sites 1 and 2, but significantly lower at the driest site 3. The growth rate had a significant positive correlation with KPY, MOR and MOE and a negative correlation with VS, but no significant impact on wood density in both groups of clones. Genotype×environment interaction (G×E) was evident in most traits due to the difference in response of clones to moisture availability. Since wood density was negatively correlated to KPY, it has to be kept at an optimum level for the profitability of pulp industry. There was no significant difference between EC and EH clones for most traits except VS at site 3. Stability of clones varied across sites in different traits, and hence clones may be selected for deployment at each site by screening for growth, followed by wood density, considering the relationship of growth and density with other traits required by pulp and solid wood industry sectors.