• Microbiology and Biotechnology Letters
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• v.24 no.6
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• pp.678-686
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• 1996

To improve the preservation of Kimchi, we isolated Lactobacillus plantarum lytic enzyme-producing strain from soil, and the enzyme was purified and characterized. From the observation of cultural and morpho- logical characteristics, the isolated strain was identified as Metarrisium anisopliae (Metschn.) Sorok. The enzyme was purified to 75-folds with 40% yields through affinity adsorption and CM-Sephadex C-50 column chromatog- raphy. The optimum pH and temperature for lytic activity are 4.0 and 40$\circ$C, respectively, and the enzyme acitvity is stable between pH 3.0 and 9.0, and up to 50$\circ$C. The enzyme is a monomeric protein with molecular weight of 40,000 daltons by SDS-PAGE and gel filtration. The enzyme is endopeptidase which breaks the peptide linkage of Lactobacillus plantarum peptidoglycan. The lytic action spectra confirmed that Leuconostoc mesenteroides, a useful strain for the fermentation of Kimchi, is not lysed by the enzyme. The enzyme activity is inhibited by N-bromosuccinimide (NBS), which probably indicates the involvement of tryptophan residue in active site of the enzyme, and also inhibited by Ag$^{+}$. The amino acid composition analysis showed that the enzyme contains more acidic amino acids than basic ones, and composition of alanine, glycine, proline and tyrosines was very high.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.705-712
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• 2005

Among soft ground treatment methods with granular soil used in domestic, the sand compaction pile method has been utilized greatly, but, as a result of exhaustion of sand and increase of unit cost, a necessity of an alternative method is suggested. In this study, the static load tests for group crushed-stone compaction piles which were constructed at in-situ site were performed. Pile diameter was 700mm and area of loading plates were changed. The static load tests of single and group piles were performed for area replacement ratio of 20, 30 and 40%. Based on test results, bearing capacity of group crushed-stone compaction pile were estimated. The more both single pile and group pile increase, the more yield bearing capacity tended to increase. Also, the yield bearing capacity of a group pile is about 50% less than the yield bearing capacity of a single pile. If the ground reinforced with the crushed-stone compaction pile is replacement ratio of $20{\sim}40%$, RIYB of both single pile and group pile increases qualitative tendency of linear more than original ground

• Journal of the Korean Geotechnical Society
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• v.27 no.9
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• pp.55-66
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• 2011

The compulsory replacement method for soft ground treatment is simple but excellent in economic feasibility. However, the accurate replacement depth is not easy to properly predicted since an theoretical algorithm has not presently been established so far. In this research a prediction equation is proposed in a new form based on the liquid limit and natural moisture content rather than on the bearing capacity of the soft soil layer. The equation is based on the monitoring as well as the confirmatory boring at the site. In addition, the equation has been derived from the data obtained from the analysis of the characteristics of silt/clay of Sihwa region. The final prediction equation has been drawn by applying the regression analysis method.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.9 no.1
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• pp.71-78
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• 1989

This Paper measures and analyzes ground vibrations induced during precast concrete pile-driving using diesel hammer at radii varying from 9m to 30m to evaluate effects of such vibrations associated with deep foundation piling operations near the residential of commercial areas. From this study, characteristics for attenuation and frequency of the vibrations casued by pile-driving are established and the empirical equation for predicting peak velocity and acceleration levels are obtained. This equation can be used to predict the peak vibration levels and select the appropriate hammers for future projects where similar soil conditions to this test site are encountered.

• 한국지구물리탐사학회:학술대회논문집
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• 2003.11a
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• pp.529-531
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• 2003

In order to investigate the level of heavy metal contamination and the seasonal variation of metal concentrations in soils and sediments influenced by past mining activities, tailings, soil and sediment samples were collected from the Hwacheon mine in Korea. The main pollution sources in this mine site are suggested as tailings and mine waste rocks. Elevated levels of Cd, Pb and Zn were found in soils and sediments. In a study of seasonal variation on the heavy metals in soils and sediments, heavy metals were higher enriched collected from before rainy season ($2^{nd}$ sampling) than after rainy season ($1^{st}$ sampling). Also, in order to estimate the microbial effects on Cd speciation in sediments, bacteria which can adsorb Cd was isolated and Cd adsorption characteristics of isolated bacteria in Cd solution was evaluated. The Cd bioremoval efficiency in Cd solution (5 ppm) by bacteria was more than $90\%$. Bioremoval efficiency in single metal solution was higher than that in mixed metal solution of Pb and Zn.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.273-281
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• 1999

In this study, linear programming (LP) was applied to solving for optimal harvesting schedules of multiple-use forest management in Mt. Kari area managed by Chunchun National Forest Station. Associated with the geographic characteristics, the study area was classified into 4 large management units or watersheds and simultaneously applied were the site-specific levels of management constraints : nondeclining yield, initial cut for existing stands, % cut area, the volume of soil erosion, timber production and carbon storage, ending inventory condition and % area species selection for regeneration. The problem was formulated using both Model I and Model II techniques. In this paper, the formulations are presented and the results of the optimal solutions are discussed for comparison purposes.

• Proceedings of the Korean Geotechical Society Conference
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• 1992.12a
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• pp.33-66
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• 1992

Recently, waste landfills built on seashores have been increased because of the easy availability of broad area near the urban communities. To evaluate the performance of the marine clay landfill liner numerical contaminant transport analyses are performed by selecting the typical section of a waste landfill built on seashores and using hydraulic conductivity data obtained from the site. Also, the laboratory electrical resistivity test and the in-situ corrosion test are performed in order to analyze the influence of the soil and leachates composing the landfills on the construct ion materials. From the results of contaminant transport analyses, it is shown that the leachates can be migrated faster through narrow pervious channels than the wide homogeneous pervious tedium and the importance of good quality barriers to prevent the contaminant migration is recognized. In the laboratory electrical resistivity test all the earth materials except the cover soils saturated with distilled water have small resistivities, which shows a high potential of corrosivity of soils composing landfills. However, the degree of corrosion of specimens buried in the landfills was not so severe except the zinc and carbon steel specimens. This apparently conflict results present the necessity of the investigation of other major factors and the long term in-situ corrosion test.

• 보존과학연구
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• s.24
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• pp.99-130
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• 2003

This research carried out composition analysis the subject of potteries from kiln site at Gangwon-do, Chungcheong Nam∙Buk-do and the followings are the results. Firstly, XRD analysis results showed that soft potteries were consisted of $\alpha$-quartz, feldspar and soil minerals while hard potteries were consisted of high temperature crystals like mullite, tridymite and cristobalite. Secondly, the firing temperatures of the soft potteries were determined using XRD analysis. It ranged from $550^{\circ}C$ to $870^{\circ}C$.While the firing temperatures of the hard potteries were divided into 3 groups; Group Ⅰ: Potteries with Mullite and Feldspar - ranged from $1000^{\circ}C$ to$1200^{\circ}C$. Group II : Potteries with Mullite and Tridymite - ranged from $875^{\circ}C$ to $1200^{\circ}C$. Group III : Potteries with Cristobalite - ranged above $1200^{\circ}C$.Thirdly, the result of correlation analysis using trace element such asSc, Rb of the potteries form Gangwon do and Chungcheong Nam.Bukdokilns allowed us to divide the area with their own characteristics into 4-groups. Using the same result, kilns sited at Chungcheong Nam.Buk-do were classified into 3-groups.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.1
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• pp.38-45
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• 1998

To extract insoluble proteins and to improve funtional properties of abolished proteins, an phytase producing Aspergillus sp. SM-15 was isolated from soil. The enzyme was purified and its enzymological characteristics were investigated. Phytase production reached to maximum when the wheat bran medium containing 1% mannose, 1% yeast extract, 1% (NH4)2HPO4 and 0.2% calcium chloride was cultured for 4 days. Phytase was purified 17.1 fold and specific activity was 244.32unit/mg by a sequencial process of ammonium sulfate fraction, ion exchange chromatography and gel filtrations Pruified enzyme was confirmed as a single band by the polyacrylamide gel electro-phoresis. The molecular weight of phytase was estimated to be 46,000. The optimum pH and temperature for the phytase activity were 5.5 and 5$0^{\circ}C$. The enzyme is stable in pH 4.5~5.5, 6$0^{\circ}C$. The activity of purified enzyme was inhibited by Hg2+ whereas activited by Pb2+ and Fe2+. The activity of phytase was inhibited by the treatment with iodine. The result indicate the possible involvement of histidine at active site. Km and Vmax of the puridied phytase were 37.037mM/L and 159.87umol/min, respectively.

• Journal of Industrial Technology
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• v.29 no.A
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• pp.9-17
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• 2009

The main purpose of this work is to analyse damages caused by debris flows during the heavy rainfall at Inje province in Kangwondo, Korea. A series of site investigations have been performed to survey the characteristics of debris flows occurred during the summer season of 2006. It has been found that major losses and costs are triggered by discharge of soil and rock fragments from landslides. During the rainfall unexpectedly high precipitation rate of 113.5mm/hour and 355mm/day was recorded, which could happen at a 80-500 year period. Comparing the period of the rainfall with the time of the landslides, it has been found that the occurrence of the landslides is directly related to heavy rainfalls. At present, several debris barriers have been built at the valleys and natural slopes have been protected by the seed spray method. It is intended to propose an appropriate solutions of restoration of landslide damages and maintenance based on findings from the current study.