• 한국미생물·생명공학회지
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• 제13권4호
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• pp.321-327
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• 1985

색소를 형성하지 못하는 Serratia sp. LW-1 균주의 균체의 protease생성조건을 검토하고 효소를 간단한 방법으로 정제하였다. 효소 생산을 위한 최적온도는 brain heart infusion배지에서 $25^{\circ}C$이었으며, 배양후 76-80시간에 최고의 균체의 효소활성을 나타냈다. Aeration효과는 5$\ell$용 flask에 배지량을 1$\ell$주입하여 180 cycles/min으로 진탕배양 하였을 때가 aeration하지 않았을 경우보다 약 8배의 균체의 효소생산을 보였다. 효소의 정 제는 ammonium sulfate침 전, ammonium sulfate분별염석 및 두번의 DEAE-cellulose column 크로마토그라피에 의하여 수행하였으며, 정제된 효소는 정제도가 약 100배, 회수율이 16%이었다. 정제된 효소는 analytical ultracentrifuge pattern에서 단일 단백질로 나타났으며 최고 활성을 나타내는 pH는 vitamin free casein을 substrate로 사용하였을 때 pH 8.5-9.5이었고 최적온도는 4$0^{\circ}C$ 근처이었다

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.

• 한국치위생학회지
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• 제20권3호
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• pp.243-256
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• 2020

Objectives: This study aimed to determine and facilitate provisions for the unmet dental needs of Koreans, stratified by time, influencing factors, and research trends, through a systematic review of related published studies since 2006. Methods: this review focused on previous studies published between January 2006 and November 2019 that analyzed influencing the factors underlying the unmet dental needs of koreans. We followed the guidelines set for each phase of research and selected the final 32 studies that met the selection criteria for the analysis. Results: The number of studies has rapidly increased since 2015 (22 studies, 68.7%). the were 68.9% in 2006, 25.9% in 2009, 41.3% in 2010- 2012, and 33.3% in 2013-2015 for adults and 27.9% in 2010, 24.6% in 2015, and 16.1% in 2017 for the rates of older adults. the rates of unmet dental needs related to economic factors, were 38.6% in 2006, 41.4% in 2007-2009, and 35.9% in 2013-2015 for adults and 50.5% in 2010 and 41.2% in 2015 for the older adults. There were common influencing factors for unmet dental needs. the rate of unmet dental needs was increased by with female gender, younger age, single marital status, low family income, low educational level, worsened subjective health condition, and the presence of chronic diseases. Conclusions: Standardized studies with more accurate definitions and assessment tools are required. however, our study emphasizes the need for a policy intervention that accounts for the characteristics of subjects to reduce unmet dental needs.

• 조명전기설비학회논문지
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• 제20권6호
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• pp.69-74
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• 2006

[ $Mg_2Ni$ ] 합금 및 $Mg_2Ni$와 카본 혼합물 입자의 수소저장 특성에 대한 기계적 분쇄(MG, Mechanical Grinding) 처리 효과를 고온 가스상의 PCT 측정 및 전기화학적인 마이크로 전극 측정법 등에 의해 검토되었다. PCT 측정은 약 $300[^{\circ}C]$의 고온에서 실시되었으며 전기화학적인 실험은 카본-섬유로 구성된 마이크로 전극을 1M KOH 수용액 속에서 조정자를 사용하여 MG 처리한 합금 단일입자에 접촉시켰다. 그 결과 $Mg_2Ni$ 합금과 카본 혼합물 입자의 경우 가스상에서 수소 해리압이 감소하고 상온에서 전기화학적인 수소화 특성이 크게 개선되었다. 이것은 기계적 분쇄(MG) 작용에 의한 합금의 미세화 및 나노화에 기인한다고 판단된다. 즉 고온 가스상의 PCT 측정 결과 수소 해리압이 MG 처리에 의해 0.55[MPa]에서 0.42[MPa]로 감소하였으며 동일 샘플 입자에 대해 마이크로 전극에 의한 평가에서도 수소화 피크가 보다 분명하게 관찰되었다.

• 조명전기설비학회논문지
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• 제27권3호
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• pp.100-106
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• 2013

In this paper, in order to analyze lightning impulse response characteristics in combined installations of SPDs and RCDs, surge protection coordination between SPDs and RCDs are experimentally investigated by using the combination wave generator. Six different types of single-phase residual current operated circuit-breakers with integral overcurrent protection for household and similar uses(RCBOs) being present on the domestic market are tested according to KS C IEC 61009-1 standard. As a result, when a class I SPD is located on the source side of an RCBO, all kinds of specimens are able to provide the proper coordination between the SPD and RCBOs without nuisance tripping, unintended operation or damage due to test impulse currents. However, in the case that the class II SPD is located on the load side of RCBOs, a lot of L-N mode injected currents is split into the RCBO, and a few RCBOs are damaged. Coordination between SPDs and RCDs is not valid and a role of SPDs is of no use. When combining SPDs with RCDs, it is necessary to select SPDs and RCDs in consideration of the protection voltage level of metal oxide varistor embedded in RCDs.

• 전력전자학회논문지
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• 제12권3호
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• pp.267-275
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• 2007

본 논문은 현재 청정에너지의 개념으로 대두되고 있는 에너지원 가운데 기계적인 에너지로 저장하여 필요 시 꺼내어 사용할 수 있는 플라이휠 에너지 저장장치에 대한 에너지 입, 출력 모델링 및 PWM 인버터 시스템의 해석 및 제어에 관한 논문으로서, 플라이휠 저장장치 특성 및 시스템 모델링에 관해 논의한다. 재질에 따른 플라이휠의 특성과 플라이휠 에너지 저장장치의 속도 특성에 따른 전압과 전류의 변화량을 수식으로 간략화 하여 분석하고, 시뮬레이션을 통하여 플라이휠의 에너지 저장상태를 분석하였다. 또한, 부하측 전원의 이상유무에 관계없이 에너지를 공급할 수 있는 Online UPS로 사용하기 위해 PDFF 제어기법을 이용하여 전압제어 및 전류제어의 이중루프 제어로 구성된 단상 PW방식의 인버터 시스템 제작하였으며, 실험을 통하여 실제로 0.1[p.u], 1[p.u]에서의 제어되는 전압, 전류제어파형 및 THD 특성에 관하여 평가한다.

• 한국정보처리학회논문지
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• 제4권10호
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• pp.2485-2492
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• 1997

오늘날 자동차의 급격한 증가에 따른 교통 체증을 완화시키기 위해 효율적인 교차로 신호 제어기의 필요성이 대두되고 있다. 최근에는 신호등의 주기를 적응적으로 제어하기 위하여 퍼지 논리가 도입되었다. 일반적인 퍼지 제어 방식은 녹색신호에 교차로로 진입하는 차량 수와 적색신호 동안 대기하는 차량 수를 퍼지 제어의 입력 변수로 사용하여 확장시간을 조정한다. 그러나 이 방법은 가변 교통량을 갖는 교차로에는 부적합하다. 본 논문에서는 대칭적인 소속함수를 갖는 기존의 퍼지 논리 제어기들보다 적은 수의 제어규칙들을 가지면서도 교통 흐름을 보다 잘 반영하는 비대칭적인 소속함수를 갖는 새로운 퍼지 논리 제어기를 제안한다. 제안된 방법의 효율성은 단일 교차로 시뮬레이션을 통해 테스트되었다. 실험결과는 제안된 방법이 평균 지체시간, 통과 대수, 포화도면에서 우수한 성능을 보여주었다.

• 전기학회논문지P
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• 제65권3호
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• pp.194-198
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• 2016

The electric power industry have recently been building out Smart Grids, a two-way electricity grid that connects power consumers and producers to a network that enables one to respond quickly to any eventuality. The construction of a two-way electricity grid means that the power control process becomes unified, from what used to be separate processes that originate individually from the consumption phase and the production and supply phases. The role of power control that takes place within each section of the power system may be independent. However, this does not mean the independent control sections are operated individually, but are configured to meet a single target and purpose. Each control section possesses enough degree of independency to respond to eventualities that may occur within different stages of the power system, but at the same time, possesses unified system elements for the stability of the entire power system. From this perspective, Smart Grids are widely regarded as the most rational power industry operation plan. A variety of different control and communication systems can be applied for an effective deployment of Smart Grids. Recently, we have seen systems such as PMS(Power Management System) and PAS(Process Automation System) applied in the deployment of Smart Grids, which are developed from the techniques utilized in the industry. The PMS is attracting particular attention for its power operations management ability. In this study, we propose plans for improvement in the rational development of power system controls through case studies of live PMS operations.

• 대한방사선기술학회지:방사선기술과학
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• 제21권1호
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• pp.29-34
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• 1998

The purpose of simple abdomen erect projection is to see the fluid level which indicates gastrointestinal ileus or free air due to perforation. we do not have to insist on low kVp technique in simple abdomen erect position as long as we can detect the fluid level and free air shadow. Therefore, the author tried to decrease patient dose by high kVp technique and to improve the image quality due to motion artifact by reduction of exposure time. [Methods] Experiment 1. * screen/film SRO1000/HRH * exposure factor : $140\;kvp{\pm}5\;kv$ with added filters, 200 mA, 0.01 sec * phantom : Acryles : 15.0 cm(equivalent to 17 cm body thickness) 17.5 cm(equivalent to 21 cm body thickness) 20.0 cm (equivalent to 25 cm body thickness) With the exposure factor for same film density($D=0.8{\pm}0.1$) and with the materials above, we tried to find out entrance skin dose and gonad dose for both male and female. Experiment 2. Burger's phantom radiography were checked to see whether there was any change of image quality according to the kVp and the added filters. Experiment 3. Using rotating meter(self made), we examined the motion artifact and the exposure time limitation. [Results and conculution] 1. Using high voltage technique of 140 kVp with added filter, Skin dose, testicle dose and ovary dose decrease to 89.3%, 47% and 71.4% respectively compare to 70 kVp technique, 2. No great changes of Burger's phantom image has detected as from 70 kVp to 140 kVp and the air hole size of Burger's phantom over 0.028 cc(Diameter 3 mm, hight 4 mm) can be distinghished. 3. 0.01 sec(1 pulse) exposure time is possible in the single phase full wave rectification that why we can quitely reduce the unsharness caused by patient's movement.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 하계학술대회 논문집 Vol.7
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• pp.271-271
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• 2006

The structural, dielectric and piezoelectric properties of $[Bi_{1-x}(Na_{0.7-x}K_{0.2}Li_{0.1})]_{0.5}BaxTiO_3$ (BNKLBxT) ceramics were studied for the compositional range, x = 0-0.08. The samples were prepared by conventional sintering technique. The result of X-ray diffraction (XRD) suggest that $Ba^{2+}$ diffuse into the $[Bi(Na_{0.7}K_{0.2}Li_{0.1})]_{0.5}TiO_3$ (BNKLT) lattices to form a solid solution with a single phase perovskite structure. The ceramic show excellent piezoelectric and ferroelectric properties, and optimum properties measured are as follows: piezoelectric constant $d_{33}=230pC/N$, planar electromechanical coupling factor $k_p\;=\;40.3%$, remanent polarization $P_r\;=\;30\;{\mu}C/cm^2$, and coercive field $E_c\; =\;2.5\;kV/mm$, respectively.