• Plant Biotechnology Reports
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• 제4권1호
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• pp.75-83
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• 2010

Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in plants. We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice. In this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach. Transgenic tobacco plants expressing the OsBADH1 gene were generated under the control of a promoter from the maize ubiquitin gene. Three homozygous lines of $T_2$ progenies with single transgene insert were chosen for gene expression analysis. RT-PCR and western blot analysis results indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco leading to the accumulation of glycine betaine. Transgenic lines demonstrated normal seed germination and morphology, and normal growth rates of seedlings under salt stress conditions. These results suggest that the OsBADH1 gene could be an excellent candidate for producing plants with osmotic stress tolerance.

• Molecules and Cells
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• 제42권2호
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• pp.104-112
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• 2019

Tracking the fate of individual cells and their progeny through lineage tracing has been widely used to investigate various biological processes including embryonic development, homeostatic tissue turnover, and stem cell function in regeneration and disease. Conventional lineage tracing involves the marking of cells either with dyes or nucleoside analogues or genetic marking with fluorescent and/or colorimetric protein reporters. Both are imaging-based approaches that have played a crucial role in the field of developmental biology as well as adult stem cell biology. However, imaging-based lineage tracing approaches are limited by their scalability and the lack of molecular information underlying fate transitions. Recently, computational biology approaches have been combined with diverse tracing methods to overcome these limitations and so provide high-order scalability and a wealth of molecular information. In this review, we will introduce such novel computational methods, starting from single-cell RNA sequencing-based lineage analysis to DNA barcoding or genetic scar analysis. These novel approaches are complementary to conventional imaging-based approaches and enable us to study the lineage relationships of numerous cell types during vertebrate, and in particular human, development and disease.

• 대한한의학방제학회지
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• 제31권4호
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• pp.389-413
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• 2023

Objectives : This study aimed to review the current trends in experimental studies on the use of natural products for treatment of gastroesophageal reflux disease (GERD). Methods : Experimental studies assessing the efficacy of natural products against GERD were searched on PubMed. Articles were selected based on predefined inclusion and exclusion criteria and then analyzed for experimental methods, interventions, and result analysis techniques. Results : A total 37 studies were included in this review. Predominantly, in vivo experiments were conducted to induce GERD through surgery, involving the ligation of the pylorus and the transitional junction between the corpus and the forestomach using 7-week-old male Sprague-Dawley rats. The acute induction model, sacrificing animals after a single administration following GERD induction, was mainly used.The utilization of cell experiments was relatively infrequent, with a focus on assessing antioxidant and anti-inflammatory effects via the treatment of the RAW 264.7 cell line with lipopolysaccharides treatment. Glycyrrhizae Radix et Rhizoma, Pinelliae Tuber, Ginseng Radix and Zingiberis Rhizoma were used as single ingredients, and herbal formula, STW-5 (iberogast), Rikkunshito (六君子湯), Banhasasim-tang (半夏瀉心湯), and Hewei Jiangni granule (和胃降逆湯) were used. Outcome analysis methods encompassed Macroscopic evaluation, esophageal function assessment, blood biomarker analysis, histological examination, protein analysis, gene expression analysis, and gastric juice analysis. Proton pump inhibitors were predominantly employed as positive controls. Conclusions : This study revealed the current trends in non-clinical research evaluating natural products for GERD. Based on the results of this study, we expect that non-clinical research on clinically effective natural products will be revitalized.

• 대한전자공학회논문지TC
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• 제44권7호통권361호
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• pp.47-52
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• 2007

본 논문은 다중 셀 환경에서 MMSE 수신기를 가지는 MIMO MC-CDMA시스템의 출력 SINR을 점근적으로 분석한다. 단일 셀에서의 점근적 성능 분석이 다중셀 환경으로 확장 적용된다. 점근적 분석을 위한 Haar 유니터리 코드의 사용은 다른 셀로부터의 간섭성분이 대각성분들의 값이 다른 대각행렬로 나타나게 한다. 본 논문에서는 다른 셀의 코드 간섭 성분을 mean square측면에서 간섭의 전력으로 수렴함을 보이고, 셀간 간섭 성분이 주어질 때 점근적으로 특정 SINR값을 찾는다. 다중 셀에서의 거리에 따른 느린 페이딩을 로그노말 분포를 가정하여 구한 이론적인 비트오차 확률과 실험을 비교하여 비슷함을 보이고, 점근적 성능에 의한 데이터 전송 수율의 셀 반경에 따른 성능을 보인다.

• Tuberculosis and Respiratory Diseases
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• 제80권2호
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• pp.179-186
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• 2017

Background: Although the World Health Organization (WHO) classification of lung squamous cell carcinoma (SCC) was revised in 2015, its clinical implications for lung SCC subsets remain unclear. We investigated whether the morphologic characteristics of lung SCC, including keratinization, were associated with clinical parameters and clinical outcome of patients. Methods: A total of 81 patients who underwent curative surgical resection of diagnosed lung SCC, were enrolled in this study. Attributes such as keratinization, tumor budding, single cell invasion, and nuclear size within the tumor, as well as immunohistochemistry of Bcl-xL and pS6 expressions, were evaluated. Results: The keratinizing and nonkeratinizing subtypes did not differ with respect to age, sex, TNM stage, and morphologic parameters such as nuclear diameter, tumor budding, and single cell invasion at the tumor edge. Most patients with the keratinizing subtype (98.0%) had a history of smoking, whereas the nonkeratinizing group had a relatively higher proportion of never-smokers relative to the keratinizing group (24.0% vs. 2.0%; p=0.008, chi-square test). Expression of pS6 (a surrogate marker of mammalian target of rapamycin complex 1 [mTORC1] signaling that regulates keratinocyte differentiation), and Bcl-xL (a key anti-apoptotic molecule that may inhibit keratinization), did not correlate significantly with the presence of keratinization. Patients with the keratinizing subtype had a significantly shorter overall survival (85.2 months vs. 135.7 months, p=0.010, log-rank test), and a multivariate analysis showed that keratinization was an independent, poor prognostic factor (hazard ratio, 2.389; 95% confidence interval, 1.090-5.233; p=0.030). Conclusion: In lung SCC, keratinization is associated with a poor prognosis, and might be associated with smoking.

• 대한기계학회논문집B
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• 제36권9호
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• pp.901-905
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• 2012

본 논문에서는 다수의 전기장 분포가 생성되는 단일 미세유로를 이용한 폐암세포 전기천공 및 활성도 분석칩을 제안하였다. 종래의 세포 전기천공 분석칩은 다수의 전기장 분포를 형성하기 위해 다수의 전극패턴 또는 다수의 미세유로를 필요로 하여 구조가 복잡하였다. 반면, 제안된 세포 전기천공 및 활성도 분석칩은 한 쌍의 전극 사이에서 계단 형상으로 폭이 변화하는 단일 미세유로를 이용하여 다수의 전기장 분포를 형성함으로써 간단한 구조로 세포 전기천공 및 활성도를 분석할 수 있다. 제안된 세포 전기천공 및 활성도 분석칩은 0.3kV/cm에서 0.5kV/cm까지 5단계의 전기장이 발생되도록 설계하였다. A549와 H23의 두 종류의 비소세포 폐암세포주를 이용한 성능실험 결과, 활성을 유지하면서 전기천공된 세포의 비율이 0.4kV/cm의 전기장에서 각각 $26.6{\pm}0.7%$ 및 $51.4{\pm}3.0%$로 가장 높은 값을 보였다. 제안된 세포 전기천공 및 활성도 분석칩은 세포의 형질주입 연구를 위한 집적화된 세포칩으로 응용될 수 있다.

• 대한수의학회지
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• 제44권1호
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• pp.41-47
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• 2004

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${\gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.

• Journal of Veterinary Science
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• 제24권3호
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• pp.43.1-43.8
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• 2023

Background: Meloxicam is used widely for exotic animal analgesia, but its toxicity in common raptor species in Thailand is unclear. Objectives: This study evaluated the single-dose effect of intramuscular meloxicam in common raptor species in Thailand for short-term and long-term periods. Methods: Twenty-two raptors were administered a single 1 mg/kg dose of meloxicam individually via intramuscular injection. The following were evaluated: clinical appearance, body weight, body condition score, body temperature, fecal appearance, complete blood cell count, and biochemistry panel before (day 0) and after the injection (1, 7, and 30 days). The collected samples were categorized into three groups: Brahminy kite (Haliastur indus) (n = 10), adult Barn owl (Tyto javanica) (n = 4), and juvenile Barn owl (n = 8). Results: None of the raptors in the study groups showed any abnormalities. The hematological profiles were significantly different in the short-term period (day 1 and day 7). The creatinine, aspartate aminotransferase, and creatinine kinase increased in several groups. On the other hand, the packed cell volume decreased in the Brahminy kite and juvenile Barn owl groups. According to the findings, an intramuscular injection of 1 mg/kg meloxicam affected the blood biochemistry panel of the muscle, but the affected raptors recovered within one week. Conclusions: An intramuscular injection of meloxicam at a single 1 mg/kg dose in Brahminy kites and Barn owls was not associated with the morbidity, hepatotoxicity, gastrointestinal toxicity, and nephrotoxicity in the short- and long-term periods.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.4959-4962
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• 2012

The objective of this retrospective study was to investigate prognostic factors associated with survival of patients with extensive stage small cell lung cancer (ES-SCLC). Included were 200 patients admitted to the Liberation Army General Hospital with a diagnosis of ES-SCLC. The demographics of patients, disease characteristics, pre-treatment biochemical parameters and therapeutic plan were assessed or evaluated. Univariate analysis found that second-line chemotherapy, radiotherapy, and no liver metastasis were associated with improved survival. Tumor response to first-line chemotherapy and normal initial hemoglobin levels were also associated with a survival benefit (all P-values ${\leq}$ 0.0369). Multivariate Cox regression analysis indicated that liver metastasis and the total number of all chemotherapy cycles were independent prognostic factors of survival. The morbidity risk in patients with liver metastasis was 2.52-fold higher than that in patients without liver metastasis (hazard ratio (HR)=2.52 (1.69-3.76); P<0.0001). However, one unit increase in the total number of chemotherapy cycles decreased the risk of death by 0.86-fold (HR=0.86 (0.80-0.92); P<0.0001). Absence of liver metastasis and ability of a patient to receive and tolerate multiple lines of chemotherapy were associated with longer survival.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 C
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• pp.1850-1852
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• 2000

In this paper, new Partial Discharge (PD) detection technique using Pockels cell was proposed and considerable apparent chaotic characteristics were discussed. For this purpose, PD was generated from needle-plane electrode in air and detected by optical measuring system using Pockels cell, based on Mach-Zehnder interferometer, consisting of He-Ne laser, single mode optical fiber, 50/50 beam splitter and photo detector. A qualitative analysis was carried out by drawing Return map for the normalized time series of the detected PD signals. The results are as follows:(a) Fixed points, between 0.7 and 1.0, are appeared clearly in the right upper area of the return map as the increase in the number of obtained data.(b) Considerable periodicity have been remarked even though exact period and length can not be determined.(c) The self-similarity can be also observed inasmuch as the late paths do not follow the previous ones. Accordingly, exact quantitative analysis such as embedding dimension, fractal dimension, and Lyapunov exponents should be carried out for deducing the quantitative properties regarding PD phenomena.