• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2001.10b
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• pp.176-176
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• 2001

• Journal of Electrical Engineering and Technology
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• v.11 no.2
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• pp.367-376
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• 2016

This paper presents a cell balancing method for a single switch flyback converter with a multi-winding transformer. The conventional method using a flyback converter with a multi-winding transformer is simple and easy to control, but the voltage of each secondary winding coil might be non-uniform because of the unequal effective turn-ratio. In particular, it is difficult to control the non-uniform effect using turn-ratios because secondary coil has a limited number of turns. The non-uniform secondary voltages disturb the cell balancing procedure and induce an unbalance in cell voltages. Individual cell control by adding a switch for each cell can reduce the undesirable effect. However, the circuit becomes bulky, resulting in additional loss. The proposed method here uses the conventional flyback converter with an adjustment made to the output filters of the cells, instead of the additional switch. The magnitude of voltage applied to a particular cell can be reduced or increased according to the adjusted filter and the selected switching frequency. An analysis of the conventional converter configuration and the filter design method reveals the possibility of adequate cell balancing control without any additional switch on the secondary side.

• 한국신재생에너지학회:학술대회논문집
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• 2006.06a
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• pp.96-98
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• 2006

Proper water management is vital to achieve high performance and durability of PEFC (Polymer Electrolyte Fuel Cell). The effects of the residual water from PEFC after purge in shut-down processes on GDL/MEAs were investigated with freeze/thaw cycles Freeze/thaw cycle tests were conducted with single cells which were designed from transparent acryl plates. Single cells which contain several amount of residual water were cycles from $80^{\circ}C$ to $-28^{\circ}C$. The resistance changes of the single cells which have various amount of residual water were evaluated by ac-impedance analysis with 24 times of freeze/thaw cycles. Also, after the freeze/thaw cycles, the property changes were characterized by visual methods such as SEM, EPMA. Though it was difficult to observe noticeable property changes in the visual characterizations, the resistance of cells dramatically increased with the amount of remained water.

• Proceedings of the KSR Conference
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• 2005.11a
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• pp.229-234
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• 2005

Thin-walled multicell box girders subjected to an eccentric load can he produced the three global behaviors of flexure, torsion, and distortion. Specially in railway bridges subjected to much eccentric load, it is quite important to evaluate influences of torsion and distortion. But it is very difficult to evaluate each influences of major behaviors numerically. If we can decompose an eccentric load P into flexural, torsional, and distortional forces. we can execute quantitative analysis each influences of major behaviors. Decomposition of Applied Load for Thin-walled Rectangular multi-cell box girders is reserched by Park, Nam- Hoi(Development of a multicell Box Beam Element Including Distortional Degrees of Freedom, 2003). But researches about trapezoidal multi-cell section is insufficient. So, this paper deals with multi-cell trapezoidal box girders. An expanded method, which is based on the force decomposition method for a single cell box girder given by Nakai and Yoo, is developed herein to decompose eccentric load Pinto flexural, torsional, and distortional forces. Derive formulas by decomposition of eccentric load is verified by 3D shell-modelling numerical analysis.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.847-859
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Korean Chemical Engineering Research
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• v.50 no.4
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• pp.743-748
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• 2012

This study presents the effects of micro-geometries on the swimming behavior of Pseudomonas aeruginosa. First, we have measured parameters of single-cell motility including cell speed, run duration time, and tumble angle under two dimensional space. The results are used to calculate motility coefficients in the width of microchannels ranging from 10 to $100{\mu}m$. Since the single-cell motility parameters measured depend on the interaction of flagella with the microchannel wall, the duration time of the running cell in restricted geometries is distinctively different. Therefore, the motility of bacteria is decreased by restricted geometries. This study suggests that microfluidic approach is useful tool for the analysis of bacterial motility under the restricted space and rapid analytical tool.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2006.05a
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• pp.927-930
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• 2006

While Intel has been increasing its exclusive possession in the system IC semiconductor market, IBM, Sony, and Toshiba founded an alliance to develop the next entertainment multi-core processor, which is named CELL. Cell is designed upon the Power architecture and includes 8 SPE (Synergistic processor Element) cores for data handling, and supports SIMD architecture for optimal execution of multimedia, or game applications. Also, it includes expanded Power microarchitecture. In this paper, we analyzed and researched the Cell microprocessor, which is evaluated as the most powerful processor in this era.

• IMMUNE NETWORK
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• v.13 no.2
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• pp.43-54
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• 2013

Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells modulating the immune network. These advances significantly support highthroughput and high-content analyses and enable an integrated understanding of the cellular and molecular interactions that underlie complex biological systems.

• Applied Microscopy
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• v.46 no.3
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• pp.134-139
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• 2016

Detailed structural and molecular imaging of intact organs has incurred academic interest because the associated technique is expected to provide innovative information for biological investigation and pathological diagnosis. The conventional methods for volume imaging include reconstruction of images obtained from serially sectioned tissues. This approach requires intense manual work which involves inevitable uncertainty and much time to assemble the whole image of a target organ. Recently, effective tissue clearing techniques including CLARITY and ACT-PRESTO have been reported that enables visualization of molecularly labeled structures within intact organs in three dimensions. The central principle of the methods is transformation of intact tissue into an optically transpicuous and macromolecule permeable state without loss of intrinsic structural integrity. The rapidly evolving protocols enable morphological analysis and molecular labeling of normal and pathological characteristics in large assembled biological systems with single-cell resolution. The deep tissue volume imaging will provide fundamental information about mutual interaction among adjacent structures such as connectivity of neural circuits; meso-connectome and clinically significant structural alterations according to pathologic mechanisms or treatment procedures.

• Animal cells and systems
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• v.7 no.2
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• pp.159-164
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• 2003

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2.8 kb was detected by Northern blot analysis, The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV-inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60％ identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.