• Journal of Korean Association for Spatial Structures
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• v.19 no.1
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• pp.45-51
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• 2019

In this study, the static load test and the load transfer test were carried out to evaluate the structural performance of the circular anchorage proposed by the previous study. Specimens were fabricated according to KCI-PS101 and ETAG 013. As a result of the static load test, it was verified that the displacement of the wedge and the strand was kept constant when the tensile force of 80% of the nominal strength of the strand was applied. In the load transfer test, it was confirmed that all the specimens satisfied the stabilization formula of KCI-PS101 and ETAG 013. Post-tensioned one-way slab with circular anchorage were fabricated to evaluate the flexural behavior. All specimens exhibited the same flexural behavior and maximum load. However, the specimen with circular anchorage were advantageous than the rectangular anchorage one in terms of crack control of the anchorage zone.

• Journal of Applied Biological Chemistry
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• v.44 no.4
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• pp.163-166
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• 2001

Piezoelectric (PZ) crystal biosensor system was used to detect the DNA of food pathogenic Listeria monocytogenes. L. monocytogenes-specific DNA was multiplied via the polymerase chain reaction using LM1 oligonucleotide (5'-TTACGAATTAAAAAGGAGCG-3') and LM2 oligonucleotide (5'-TTAAATCAGCAGGGGTCTTT-3') as primers. DNA fragment of 161 bp, which was specific only for L. monocytogenes, was observed. To obtain a large amount of single-stranded DNA containing an SH group used for coupling to the gold electrode chemisorptively, LM1 oligonucleotide containing a mercaptohexyl group was utilized as a single strand PCR primer. The PCR product was immobilized onto the gold electrode of PZ crystal, and hybridization was monitored in quartz crystal microbalance (QCM) system by injecting the antisense single-stranded DNA of 161 nucleotides obtained via the single strand PCR using the unmodified LM2 primer. Approximately 70 Hz of frequency drop was observed in the QCM system in the case of two consecutive injections of $5{\mu}g$ of the antisense single-stranded DNA.

• Journal of the Korean Chemical Society
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• v.55 no.5
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• pp.887-891
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• 2011

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.276-276
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• 2013

Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (~104) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

• Journal of Microbiology
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• v.44 no.5
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• pp.508-514
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• 2006

In this study, the double-stranded DNA-dependent activities of Deinococcus radiodurans RecA protein (Dr RecA) were characterized. The interactions of the Dr RecA protein with double-stranded DNA were determined, especially dsDNA-dependent ATP hydrolysis by the Dr RecA protein and the DNA strand exchange reaction, in which multiple branch points exist on a single RecA protein-DNA complex. A nucleotide cofactor (ATP or dATP ) was required for the Dr RecA protein binding to duplex DNA. In the presence of dATP, the nucleation step in the binding process occurred more rapidly than in the presence of ATP. Salts inhibited the binding of the Dr RecA protein to double-stranded DNA. Double-stranded DNA-dependent ATPase activities showed a different sensitivity to anion species. Glutamate had only a minimal effect on the double-stranded DNA-dependent ATPase activities, up to a concentration of 0.7 M. In the competition experiment for Dr RecA protein binding, the Dr RecA protein manifested a higher affinity to double-stranded DNA than was observed for single-stranded DNA.

• Journal of the Korean Chemical Society
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• v.52 no.6
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• pp.630-636
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• 2008

• The Plant Pathology Journal
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• v.18 no.6
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• pp.308-312
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• 2002

Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf（MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100％ homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.164-168
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• 2002

Phthalate analogues are a plasticizer and solvent used in industry and were reported to be a potential carcinogen classified in the category of suspected endocrine disruptors. Most common human exposure to these compounds may occur with contaminated food. They may migrate into food from plastic wrap or may enter food from general environmental contamination. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of phthalates that possibly threaten the public health. To determine whether seven phthalate analogues i.e. diallyl phthalate, diisodecyl phthalate, di-n-nonyl phthalate, butyl benzyl phthalate, di-n-octyl phthalate, di-tridecyl phthalate, and dibutyl phthalate, can induce DNA strand breakage that is one of the various factors related to the mechanism of carcinogenicity, the comet assay which has been widely used for the detection and measurement of DNA strand breaks, was conducted in L5178Y mouse lymphoma cells. From these results, seven phthalates revealed dose-dependent decrease of cell viability, however, no remarkable cytotoxicity was observed even at high concentration of 100 $\mu\textrm{g}$/$m\ell$ phthalates. And also, the results showed that the induction of DNA strand breaks by seven phthalates was not significantly different from the control in this study.

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.338.2-338
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• 1995

No Abstract, See Full Text

• Journal of the Korean Wood Science and Technology
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• v.28 no.3
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• pp.1-8
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• 2000

To develop the technology of producing structural board from low grade materials, an attempt was made to produce strand/particle composites from split wood strand(S) and particle(P) of (Cryptomeria japonica D. Don), which changed the layer construction and the ratio of S/P. The influence of layer construction on board properties was determined, focusing on the number and alignment of the S layers. The effect of weight ratio of S/P (3:7, 1:1, 7:3) on mechanical properties was also discussed on seven layered panel. Mechanical properties were determined from static bending tests to give parallel and perpendicular modulus of rupture (MOR) and modulus of elasticity (MOE), and the internal bond (IB) strength. In general, the surface strand layers contributed to the MOR and MOE. The parallel MOR and MOE values were the largest for the single layered S panel (only Slayers: S1), but the perpendicular MOR and MOE was the smallest. Perpendicular MOR and MOE were the largest for seven layered composite that had two cross oriented strand layers (SPSPSPS: SP7). Specimens retained more than half of their MOE and MOR after two hours in boiling water and one hour soaking. IB was the largest for the panel having only P layers, however, differences in IB strength were not identified among the other multi-layered composite panels thus the effect of layer construction on IB strength was small. Thickness swelling (TS) and surface roughness were smaller for the composite having P layers on the surface than for those having S layers. The addition of strands did not enhance the mechanical properties (MOR, MOE, IB). TS values for the panels, with which the S/P ratio was over than 1:1, was the similar to the value for the single layered S panels.