• 한국공간구조학회논문집
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• 제19권1호
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• pp.45-51
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• 2019

In this study, the static load test and the load transfer test were carried out to evaluate the structural performance of the circular anchorage proposed by the previous study. Specimens were fabricated according to KCI-PS101 and ETAG 013. As a result of the static load test, it was verified that the displacement of the wedge and the strand was kept constant when the tensile force of 80% of the nominal strength of the strand was applied. In the load transfer test, it was confirmed that all the specimens satisfied the stabilization formula of KCI-PS101 and ETAG 013. Post-tensioned one-way slab with circular anchorage were fabricated to evaluate the flexural behavior. All specimens exhibited the same flexural behavior and maximum load. However, the specimen with circular anchorage were advantageous than the rectangular anchorage one in terms of crack control of the anchorage zone.

• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.163-166
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• 2001

Piezoelectric (PZ) crystal biosensor system was used to detect the DNA of food pathogenic Listeria monocytogenes. L. monocytogenes-specific DNA was multiplied via the polymerase chain reaction using LM1 oligonucleotide (5'-TTACGAATTAAAAAGGAGCG-3') and LM2 oligonucleotide (5'-TTAAATCAGCAGGGGTCTTT-3') as primers. DNA fragment of 161 bp, which was specific only for L. monocytogenes, was observed. To obtain a large amount of single-stranded DNA containing an SH group used for coupling to the gold electrode chemisorptively, LM1 oligonucleotide containing a mercaptohexyl group was utilized as a single strand PCR primer. The PCR product was immobilized onto the gold electrode of PZ crystal, and hybridization was monitored in quartz crystal microbalance (QCM) system by injecting the antisense single-stranded DNA of 161 nucleotides obtained via the single strand PCR using the unmodified LM2 primer. Approximately 70 Hz of frequency drop was observed in the QCM system in the case of two consecutive injections of $5{\mu}g$ of the antisense single-stranded DNA.

• 대한화학회지
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• 제55권5호
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• pp.887-891
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• 2011

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.276-276
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• 2013

Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (~104) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

• Journal of Microbiology
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• 제44권5호
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• pp.508-514
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• 2006

In this study, the double-stranded DNA-dependent activities of Deinococcus radiodurans RecA protein (Dr RecA) were characterized. The interactions of the Dr RecA protein with double-stranded DNA were determined, especially dsDNA-dependent ATP hydrolysis by the Dr RecA protein and the DNA strand exchange reaction, in which multiple branch points exist on a single RecA protein-DNA complex. A nucleotide cofactor (ATP or dATP ) was required for the Dr RecA protein binding to duplex DNA. In the presence of dATP, the nucleation step in the binding process occurred more rapidly than in the presence of ATP. Salts inhibited the binding of the Dr RecA protein to double-stranded DNA. Double-stranded DNA-dependent ATPase activities showed a different sensitivity to anion species. Glutamate had only a minimal effect on the double-stranded DNA-dependent ATPase activities, up to a concentration of 0.7 M. In the competition experiment for Dr RecA protein binding, the Dr RecA protein manifested a higher affinity to double-stranded DNA than was observed for single-stranded DNA.

• 대한화학회지
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• 제52권6호
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• pp.630-636
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• 2008

• The Plant Pathology Journal
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• 제18권6호
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• pp.308-312
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• 2002

Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf（MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100％ homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.164-168
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• 2002

Phthalate analogues are a plasticizer and solvent used in industry and were reported to be a potential carcinogen classified in the category of suspected endocrine disruptors. Most common human exposure to these compounds may occur with contaminated food. They may migrate into food from plastic wrap or may enter food from general environmental contamination. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of phthalates that possibly threaten the public health. To determine whether seven phthalate analogues i.e. diallyl phthalate, diisodecyl phthalate, di-n-nonyl phthalate, butyl benzyl phthalate, di-n-octyl phthalate, di-tridecyl phthalate, and dibutyl phthalate, can induce DNA strand breakage that is one of the various factors related to the mechanism of carcinogenicity, the comet assay which has been widely used for the detection and measurement of DNA strand breaks, was conducted in L5178Y mouse lymphoma cells. From these results, seven phthalates revealed dose-dependent decrease of cell viability, however, no remarkable cytotoxicity was observed even at high concentration of 100 $\mu\textrm{g}$/$m\ell$ phthalates. And also, the results showed that the induction of DNA strand breaks by seven phthalates was not significantly different from the control in this study.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.338.2-338
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• 1995

No Abstract, See Full Text

• Journal of the Korean Wood Science and Technology
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• 제28권3호
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• pp.1-8
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• 2000

본 연구는 미이용 자원인 소경목(小經木) 조생수(早生樹) 등의 저질원료로부터 고성능을 지니는 구조용 보드를 제조하는 기술을 개발하는 것을 목적으로 하여 할렬 스트랜드 (S)/파티클(P) 복합체의 층구조와 S와 P의 혼합비율을 바꾼 단면구성에 따른 기초적 물성을 검토했다. 그 결과 스트랜드층을 포함하고 있는 SP 복합체의 경우, 전체적으로 휨성능(MOR, MOE)이 매우 높았다. 또 S단층 보드는 현저한 이방성을 나타내었지만 층구조가 PB에 가까워질수록 이방성은 감소하여, 7층 구조의 복합체는 이방성이 적었으며 특히 SP7은 직교방향도 휨강도가 높았다. 습윤시 휨강도 성능도 같은 경향을 나타내었다. 박리강도(IB)는 PB가 가장 높았고, 스트랜드층을 가진 복합체는 거의 같은 값을 나타내었다. 두께팽창율(TS) 은 PB가 가장 적은 값을 나타내었으며, 표층이 P층인 구조가 S표층구조보다 적은 경향을 나타내었다. 복합체의 표면특성은 표층 엘리먼트의 영향을 받아, 적고 미세한 엘리먼트인 P표층의 복합체가 크고 두꺼운 S표층 복합체보다 양호하였다. S와 P의 혼합비율의 영향은, SP비(比)를 증가시켜도 강도물성(MOR, MOE, IB)은 향상되지 않으며, 오히려 저하되는 것도 있었다. 두께팽창율(TS)은 SP 비(比) 1:1 이상의 복합체에서는 S만으로 제조한 보드와 같은 정도의 값을 나타내었다.