• Animal Systematics, Evolution and Diversity
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• 제36권2호
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• pp.139-142
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• 2020

The family Trogossitidae Latreille occurs worldwide, including some Pacific islands. The family contains about 50 genera and 600 described species, and the group was recently classified by Kolibáč into three subfamilies, Lophocaterinae, Peltinae and Trogossitinae. Among the lophocaterine genera, a genus Ancyrona Reitter includes 62 species in most parts of the world except the Nearctic region, and is divided into five informal species groups by Kolibáč. In this study, the genus Ancyrona and its a single species, A. diversa (Pic), is newly reported in the Korean fauna. A diagnosis, habitus photographs, and illustrations of female diagnostic characters are provided, with a key to Palaearctic species of the genus Ancyrona.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.467-469
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• 2003

This research aimed to develop forest type classification technique for the mixed forest with coniferous and broad-leaved species using the high resolution satellite data. QuickBird data was used as satellite data. The method of this research was to extract satellite data for every single tree crown using image segmentation technique, then to evaluate the accuracy of classification by changing grouping criteria such as tree species, families, coniferous or broad-leaved species, and timber prices. As a result, the classification of tree species and families level was inaccurate, on the other hand, coniferous or broad-leaved species and timber price level was high accurate.

• ALGAE
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• 제35권1호
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• pp.33-43
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• 2020

Two species of the agar-yielding genus Gelidium, G. galapagense and G. isabelae, have previously been reported from Korea but their occurrence has not been confirmed with molecular data. We intensively collected samples of Gelidium from Jeju Island, where the two species were reported, and the southern coast of Korea. Phylogenetic analyses based on cox1 and rbcL sequences revealed that only a single species occurred in Korea. The Korean species was distantly related to G. galapagense and G. isabelae from the Galápagos Islands, and formed a clade with G. microdonticum, G. millarianum, and G. pakistanicum. A new species, G. palmatum, is described for those specimens that were previously recognized as either G. galapagense or G. isabelae from Korea. G. palmatum is small in size (up to 0.7 cm), with compressed, lanceolate axes, irregular, digitate to palmate branches, abundant rhizines in the medulla, tetrasporangial sori without sterile margins, and rounded bilocular cystocarps borne subapically on palmate branchlets.

• Animal Systematics, Evolution and Diversity
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• 제34권4호
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• pp.220-222
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• 2018

The family Tetratomidae Billberg occurs in most parts of the world but many species are discovered from Australia and New Zealand. The family contains 13 genera and over 150 described species in five subfamilies, Eustrophinae, Hallomeninae, Penthinae, Piseninae and Tetratominae. Among the eustrophine genera, a genus Eustrophus Illiger includes four species in the Holartic region including three Palaearctic species, E. dermestoides (Fabricius), E. niponicus Lewis and E. yunnanensis Nikitsky. In this study, the genus Eustrophus and its a single species, E. niponicus, are newly discovered from the Korean fauna. A diagnosis, habitus photographs and illustrations of diagnostic characters including aedeagus of the species are provided.

• Food Science and Biotechnology
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• 제15권5호
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• pp.664-671
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• 2006

Modeling the growth kinetics of lactic acid bacteria (LAB), one of the most valuable microbial groups in the food industry, has been actively pursued in order to understand, control, and optimize the relevant fermentation processes. Most modeling approaches have focused on the development of single population models. Primary single population models provide fundamental kinetic information on the proliferation of a primary LAB species, the effects of biological factors on cell inhibition, and the metabolic reactions associated with cell growth. Secondary single population models can evaluate the dependence of primary model parameters, such as the maximum specific growth rate of LAB, on the initial external environmental conditions. This review elucidates some of the most important single population models that are conveniently applicable to the LAB fermentation analyses. Also, a well-defined mixed population model is presented as a valuable tool for assessing potential microbial interactions during fermentation with multiple LAB species.

• The Plant Pathology Journal
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• 제35권6호
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• pp.654-661
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• 2019

The soybean cyst nematode, Heterodera glycines, is a major plant-parasitic nematode that has caused important economic losses to Korea's soybean production. Four species of cyst nematodes, H. schachtii, H. glycines, H. trifolii, and H. sojae, all belong to schachtii group are coexist in field soil in Korea. The rapid identification of the nematode is crucial for preventing crop damage and in decision making for controlling this nematode. This study aimed to develop a species-specific primer set for quantitative PCR (qPCR) assay of H. glycines. The specific primer set (HGF1 and HGR1) for H. glycines was designed based on the cytochrome c oxidase subunit I (COI) sequence of mitochondrial DNA. After optimization, it is possible to identify the H. glycines using a qPCR assay with DNA extracted from a single cyst and single second-stage juvenile (J2). The specificity was confirmed by the absence of SYBR fluorescent signals of three other Heterodera species. A serial dilution of DNA extracted from a single cyst was obtained for the sensitivity test. The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value (R² = 0.996, slope = -3.49) and that the detection limit concentration of DNA of the primer set was 10 pg of DNA per reaction. Our findings suggested that H. glycines could be distinguished from H. sojae and other Heterodera species when a qPCR assay is used with a specific primer set.

• 한국산업보건학회지
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• 제9권2호
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• pp.167-176
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• 1999

A new analytical procedure for the measurement of monomeric isocyanates and total isocyanate group in workplaces has been investigated. The method described herd involves derivatization of the isocyanate sample upon collection with the reagent 9-anthracenylmethyl 1-piperazinecarboxylate (PAC). Laboratory investigations have demonstrated that excess PAC reagent can be satisfactorily removed from PAC-derivatized monomeric isocyanates-a requirement for the success f the analytical procedure. After removal of excess PAC reagent, the PAC derivatives of butyl isocyanate, phenyl isocyanate, HDI, MDI, and TDI were reacted with sodium thiomethoxide to convert them all to 9-anthracenylmethyl methyl sulfide (AMMS). Total isocyanate group was determined by HPLC analysis and quantification of the single AMMS peak. This circumvents many of the disadvantages associated with current HPLC methods. There were no longer problems associated with quantifying late-eluting peaks and analysis times were very short. A single detector was used for quantification because a standard of the analyte existed and the retention time could be determined. Because all species were converted to a single analyte, the problem of variability of response factors among different species was averted. Finally, there were no complex chromatograms to interpret. Monomers of other individual species were measured by analysis of the sample before the individual species were converted to AMMS. The favorable performance of PAC warrants its further study as a reagent for the determination of total isocyanate group in air.

• Molecules and Cells
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• 제39권9호
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• pp.692-698
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• 2016

Advances in next generation sequencing (NGS) technologies have enabled population-level studies for many animals to unravel the relationships between genotypic differences and traits of specific populations. The objective of this study was to perform evolutionary analysis of single nucleotide polymorphisms (SNP) in genes of Korean native cattle Hanwoo in comparison to SNP data from four other cattle breeds (Jersey, Simmental, Angus, and Holstein) and four related species (pig, horse, human, and mouse) obtained from public databases through NGS-based resequencing. We analyzed population structures and differentiation levels for the five cattle breeds and estimated species-specific SNPs with their origins and phylogenetic relationships among species. In addition, we identified Hanwoo-specific genes and proteins, and determined distinct changes in protein-protein interactions among five species (cattle, pig, horse, human, mouse) in the STRING network database by additionally considering indirect protein interactions. We found that the Hanwoo population was clearly different from the other four cattle populations. There were Hanwoo-specific genes related to its meat trait. Protein interaction rewiring analysis also confirmed that there were Hanwoo-specific protein-protein interactions that might have contributed to its unique meat quality.

• Reproductive and Developmental Biology
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• 제33권4호
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• pp.229-236
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• 2009

The glycoprotein hormone family represents a class of heterodimers, that includes the placental hormone equine chorionic gonadotropin (eCG) and the anterior pituitary hormones- follitropin (FSH), lutropin (LH), and thyrotropin (TSH). The 4 hormones are heterodimers, with a common $\alpha$-subunit and unique $\beta$-subunits. eCG is the most heavily glycosylated of the known pituitary and placental glycoprotein hormones. Recent observations using single chain glycoprotein hormone analogs in which, the $\beta$-and $\alpha$-subunits are linked, implied that heterodimeric-like quaternary configuration is not a prerequisite for receptor/signal transduction. To study the function and signal transduction of tethered rec-eCG, a single chain eCG molecule was constructed and rec-eCG protein was produced. Molecular mass of the single chain is about 45 kDa. All mice were ovulated by tethered rec-eCG treatment. The dual activity of tethered rec-eCG was determined in receptor cell lines of nonequid species; in fact, this dual activity was proven in species other than horse. Tethered rec-eCG in equids does not bind to FSH receptors, suggesting that eCG is primarily an LH-like hormone in the horse. Taken together, these data suggest that tethered rec-eCG has dual activity in nonequid species in vitro. However, it has only LH-like activity in equid species in vitro.

• Journal of Ginseng Research
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• 제34권1호
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• pp.41-46
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• 2010

Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.