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Function of the Tethered rec-eCG in Rat and Equine Receptors  

Park, Jong-Ju (Animal Biotechnology, Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Jargal, Naidansuren (Animal Biotechnology, Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Yoon, Jong-Taek (Animal Biotechnology, Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Min, Kwan-Sik (Animal Biotechnology, Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Publication Information
Reproductive and Developmental Biology / v.33, no.4, 2009 , pp. 229-236 More about this Journal
Abstract
The glycoprotein hormone family represents a class of heterodimers, that includes the placental hormone equine chorionic gonadotropin (eCG) and the anterior pituitary hormones- follitropin (FSH), lutropin (LH), and thyrotropin (TSH). The 4 hormones are heterodimers, with a common $\alpha$-subunit and unique $\beta$-subunits. eCG is the most heavily glycosylated of the known pituitary and placental glycoprotein hormones. Recent observations using single chain glycoprotein hormone analogs in which, the $\beta$-and $\alpha$-subunits are linked, implied that heterodimeric-like quaternary configuration is not a prerequisite for receptor/signal transduction. To study the function and signal transduction of tethered rec-eCG, a single chain eCG molecule was constructed and rec-eCG protein was produced. Molecular mass of the single chain is about 45 kDa. All mice were ovulated by tethered rec-eCG treatment. The dual activity of tethered rec-eCG was determined in receptor cell lines of nonequid species; in fact, this dual activity was proven in species other than horse. Tethered rec-eCG in equids does not bind to FSH receptors, suggesting that eCG is primarily an LH-like hormone in the horse. Taken together, these data suggest that tethered rec-eCG has dual activity in nonequid species in vitro. However, it has only LH-like activity in equid species in vitro.
Keywords
Tethered rec-eCG; Dual activity; LH/CG receptor; FSH receptor;
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