• Title/Summary/Keyword: Single species

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The complete chloroplast genome of Zoysia macrostachya (Poaceae): Insights into intraspecific variations and species delimitation of the Zoysia species

  • OH, Sung-Dug;LEE, Seong-Kon;YUN, Doh-Won;SUN, Hyeon-Jin;KANG, Hong-Gyu;LEE, Hyo-Yeon;XI, Hong;PARK, Jongsun;LEE, Bumkyu
    • Korean Journal of Plant Taxonomy
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    • v.51 no.3
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    • pp.326-331
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    • 2021
  • The complete chloroplast genome of Zoysia macrostachya Franch. & Sav. isolated in Korea is 135,902 bp long (GC ratio is 38.4%) and has four subregions; 81,546 bp of large single-copy (36.3%) and 12,586 bp of small single-copy (32.7%) regions are separated by 20,885 bp of inverted repeat (44.1%) regions, including 130 genes (83 protein-coding genes, eight rRNAs, and 39 tRNAs). Thirty-nine single nucleotide polymorphisms and 11 insertions and deletion (INDEL) regions were identified from two Z. macrostachya chloroplast genomes, the smallest among other Zoysia species. Phylogenetic trees show that two Z. macrostachya chloroplast genomes are clustered into a single clade. However, we found some incongruency with regard to the phylogenetic position of the Z. macrostachya clade. Our chloroplast genome provides insights into intraspecific variations and species delimitation issues pertaining to the Zoysia species.

Single Particle Characterization of Aerosol Particles Collected During "Asian Dust" Storm Events in the Spring of 2000 and 2001, Using Low-Z Electron Probe X-ray Microanalysis (단일입자분석 (Low-Z Electron Probe X-ray Microanalysis)을 이용한 2000년, 2001년에 발생한 황사 입자의 특성분석)

  • 황희진;김혜경;노철언
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.4
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    • pp.415-425
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    • 2003
  • A single particle analysis, called low-Z electron probe X-ray microanalysis was applied to characterize the atmospheric aerosols collected during Asian Dust storm events in the year of 2000 and 2001. Most frequently encountered chemical species were the soil-originated species such as aluminosilicates, silicon dioxide, and calcium carbonate. Also various species such as carbon -rich, organics, sea salts, and some reacted calcium carbonate were identified. The observation of internally mixed particles oi calcium carbonate, calcium nitrate and/or calcium sulfate shows the occurrence of the heterogeneous reaction between Asian Dust particles and NO$_{x}$ and/or SO$_{x}$ species in the atmosphere.ere.

ESTIMATING CROWN PARAMETERS FROM SPACEBORNE HIGH RESOLUTION IMAGERY

  • Kim, Choen;Hong, Sung-Hoo
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.247-249
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    • 2007
  • Crown parameters are important roles in tree species identification, because the canopy is the aggregate of all the crowns. However, crown measurements with spaceborne image data have remained more difficult than on aerial photographs since trees show more structural detail at higher resolutions. This recognized problem led to the initiation of the research to determine if high resolution satellite image data could be used to identify and classify single tree species. In this paper, shape parameters derived from pixel-based crown area measurements and texture features derived from GLCM parameters in QuickBird image were tested and compared for individual tree species identification. As expected, initial studies have shown that the crown parameters and the canopy texture parameters provided a differentiating method between coniferous trees and broad-leaved trees within the compartment(less than forest stand) for single extraction from spaceborne high resolution image.

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STANDARDIZATION OF TEST ORGANISMS AND DEVELOPMENT OF TOXICITY TESTS METHODS

  • Yasuno, M.
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1993.06a
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    • pp.4-5
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    • 1993
  • Toxicity tests in our laboratory are conducted usually with mass-reared organisms. They are under the same environmentel conditions throughout seasons and are supplied at specific age. A total of 38 species of aquatic organisms are being reared. We have attempted to establish pruified strains or to select clones of various parthenogenic organisms. The merits or demerits of our culturing test organisms are discussed. The differences in the susceptibility among clones or strains of test organism are also discussed. For a single species test, algae, daphnia, fish are often used. However, we usually use early stages, but occasionally, adults fish are used for reproduction tests. As an another important aspect, the toxicity through food chains has been studied. In this study, we select a pair of species belonging different trophic levels. The differences between single species tests and multispecies tests will be discussed. Even a single species test intends to assess the effects of chemicals on ecosystem levels, however, this idea is not applicable to ecosystems. Single species tests with standard organisms and multispecies tests are contradictory in concept. One type of multispecles tests is indoor microcosms being composed of severel species artificially assembled, and another is composed of natural components (both indoor and outdoor). We have used three types of outdoor mesocosms using ponds and three types of artificial streams. The mesocosms is useful to not only to analyze the floral or faun61 changes but also to study the fate or behaviour of chemicals in naturd environments. Lastly, usefulness of the field observation or experiments or semi-field experiments will be discussed. This will enhance the exploitation of early warning systems utilizing indicator organisms or animal behaviour.aviour.

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A Study on the Multi-gear and Multi-species Fisheries Assessment Models in Korean Waters II. Single-species by Multiple Fisheries (한국 근해 복수어구 및 다종어업 자원 평가모델 연구 II. 복수어구에 의한 단일 어종 자원의 이용)

  • SEO Young Il;ZHANG Chang Ik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.4
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    • pp.359-364
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    • 2001
  • This paper presents case studies on the multi-species fisheries in Korean waters, Multi-species fisheries were divided into two types, that is, multi-species by a single fishery and single species by multiple fisheries. For the case of single species by multiple fisheries, the small yellow croaker stock caught mainly by the Korean pair trawl fishery and the Korean stow net fishery was selected. This approach uses both standardized fishing efforts for the two fisheries by a general linear model and some data for the economic analysis, and then estimates maximum sustainable yield (MSY), maximum economic yield (MEY) and fishing efforts for MSY and MEY, An analysis of interaction aspects between pair trawl and stow net fisheries was carried out to predict the optimal level of fishing effort from the economic point of view, which gives the largest benefits to the two fisheries.

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Random Amplified Polymorphic DNA Analysis of Genetic Relationships Among Acanthopanax Species

  • Park, Sang-Yong;Yook, Chang-Soo;Nohara, Toshihiro;Mizutani, Takayuki;Tanaka , Takayuki
    • Archives of Pharmacal Research
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    • v.27 no.12
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    • pp.1270-1274
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    • 2004
  • Random amplified polymorphic DNA (RAPD) analysis was used to determine the genetic relationships among seventeen species of the Acanthopanax species. The DNA isolated from the leaves of the samples was used as template in polymerase chain reaction (PCR) with twenty random decamer primers in order to distinguish plant subspecies at the level of their genomes. The RAPD patterns were compared by calculating pairwise distances using Dice similarity index, and produced to the genetic similarity dendrogram by unweighted pair-group method arithmetic averaged (UPGMA) analysis, showing three groups; a major cluster(twelve species), minor cluster (4 species) and single-clustering species. The results of RAPD were compatible with the morphological classification, as well as the chemotaxonomic classification of the Acanthopanax species. The Acanthopanax species containing 3,4-seco-lupane type triterpene compounds in their leaves corresponded to the major cluster, another species having oleanane or normal lupane type constituents to minor clusters, and one species not containing triterpenoidal compound to single-cluster.

First Report of Two Species of Mantid Shrimps (Stomatopoda: Squilloidea: Squillidae) from Korean Waters

  • Kang, Jung-Ha;Lee, Seok Hyun;Ko, Hyun Sook
    • Animal Systematics, Evolution and Diversity
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    • v.32 no.3
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    • pp.234-239
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    • 2016
  • Two species of mantid shrimps were collected from southern part of Korea and identified as Kempella mikado and Squilloides leptosquilla of the Squillidae. The former species is characterized by having a dactylus of the raptorial claw with six teeth, only the fifth thoracic somite with a single spinous lateral process, and the fifth abdominal somite with a pair of black dorsal patches. However, the latter species is characterized by having a dactylus of the raptorial claw with four teeth, and the fifth to seventh thoracic somites each with a single lateral process. Both species are described with figures and photographs. The present records are extensions of their geographical distributions and new records in Korea. Hence, a total of six species of the stomatopods have been recorded in Korea up to date: Oratosquilla oratoria, Faughnia formosae, Taku spinosocarinatus, Chorisquilla spinosissima, Kempella mikado, and Squilloides leptosquilla.

Machine Learning SNP for Classification of Korean Abalone Species (Genus Haliotis) (전복류(Genus Haliotis)의 분류를 위한 단일염기변이 기반 기계학습분석)

  • Noh, Eun Soo;Kim, Ju-Won;Kim, Dong-Gyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.4
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    • pp.489-497
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    • 2021
  • Climate change is affecting the evolutionary trajectories of individual species and ecological communities, partly through the creation of new species groups. As population shift geographically and temporally as a result of climate change, reproductive interactions between previously isolated species are inevitable and it could potentially lead to invasion, speciation, or even extinction. Four species of abalone, genus Haliotis are present along the Korean coastline and these species are important for commercial and fisheries resources management. In this study, genetic markers for fisheries resources management were discovered based on genomic information, as part of the management of endemic species in response to climate change. Two thousand one hundred and sixty one single nucleotide polymorphisms (SNPs) were discovered using genotyping-by-sequencing (GBS) method. Forty-one SNPs were selected based on their features for species classification. Machine learning analysis using these SNPs makes it possible to differentiate four Haliotis species and hybrids. In conclusion, the proposed machine learning method has potentials for species classification of the genus Haliotis. Our results will provide valuable data for biodiversity conservation and management of abalone population in Korea.

Preliminary research for molecular markers of two invasive toxic weeds, Cenchrus L. (Poaceae) species, based on NGS technique

  • Hyun, JongYoung;Jung, Joonhyung;Do, Hoang Dang Khoa;Kim, Joo-Hwan
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.71-71
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    • 2019
  • The genus Cenchrus (Poaceae), containing ca. 23 species, is distributed throughout Australia, Africa, Indian sub-continent, and America. In Korea, Cenchrus longispinus (Hack.) Fernald, especially introduced to Daecheong Island in 1999, is one of the most hazardous invasive plant which causes serious environmental threats, biodiversity damages and physically negative impact on humans and animals. Based on the next-generation sequencing (NGS) technology, we characterized the chloroplast (cp) genome sequences of C. longispinus which contains a large single copy (LSC; 80,223 bp), a small single copy (SSC; 12,449 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). Additionally, we analyzed the cp genome sequences of Cenchrus echinatus L. which contains a large single copy (LSC; 80,220 bp), a small single copy (SSC; 12,439 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). These cp genomes consist of 75 unique genes, 4 rRNA coding genes, 33 tRNA coding genes and 21 duplicated in the IR regions, of which the gene content and organization are similar to the other Poaceae cp genomes. We selected 40 potential regions in cp genomes of two Cenchrus species and one Korean Pennisetum species to develop new single nucleotide polymorphism (SNP) markers for identifying C. longispinus based on amplification-refractory mutation system (ARMS) technique. The markers, inferred from SNP in matK and ndhF genes, show effectiveness to recognize C. longispinus from C. echinatus and Korean native species Pennisetum alopecuroides (L.) Spreng.

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Evolutionary and Functional Analysis of Korean Native Pig Using Single Nucleotide Polymorphisms

  • Lee, Jongin;Park, Nayoung;Lee, Daehwan;Kim, Jaebum
    • Molecules and Cells
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    • v.43 no.8
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    • pp.728-738
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    • 2020
  • Time and cost-effective production of next-generation sequencing data has enabled the performance of population-scale comparative and evolutionary studies for various species, which are essential for obtaining the comprehensive insight into molecular mechanisms underlying species- or breed-specific traits. In this study, the evolutionary and functional analysis of Korean native pig (KNP) was performed using single nucleotide polymorphism (SNP) data by comparative and population genomic approaches with six different mammalian species and five pig breeds. We examined the evolutionary history of KNP SNPs, and the specific genes of KNP based on the uniqueness of non-synonymous SNPs among the used species and pig breeds. We discovered the evolutionary trajectory of KNP SNPs within the used mammalian species as well as pig breeds. We also found olfaction-associated functions that have been characterized and diversified during evolution, and quantitative trait loci associated with the unique traits of KNP. Our study provides new insight into the evolution of KNP and serves as a good example for a better understanding of domestic animals in terms of evolution and domestication using the combined approaches of comparative and population genomics.