• 제목/요약/키워드: Single sequence

검색결과 1,401건 처리시간 0.031초

Nucleotide and Deduced Amino Acid Sequences of Rat Myosin Binding Protein H (MyBP-H)

  • Jung, Jae-Hoon;Oh, Ji-Hyun;Lee, Kyung-Lim
    • Archives of Pharmacal Research
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    • 제21권6호
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    • pp.712-717
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    • 1998
  • The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin- binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7kDa and includes the common consensus 1CA__TG' protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III- Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains `RKPS` sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC)phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4N-myristoylation site.

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A New Method for Elimination of Zero-Sequence Voltage in Dual Three-Level Inverter Fed Open-End Winding Induction Motors

  • Geng, Yi-Wen;Wei, Chen-Xi;Chen, Rui-Cheng;Wang, Liang;Xu, Jia-Bin;Hao, Shuang-Cheng
    • Journal of Power Electronics
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    • 제17권1호
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    • pp.67-75
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    • 2017
  • Due to the excessive zero-sequence voltage in dual three-level inverter fed open-end winding induction motor systems, zero-sequence circumfluence which is harmful to switching devices and insulation is then formed when operating in a single DC voltage source supplying mode. Traditionally, it is the mean value instead of instantaneous value of the zero-sequence voltage that is eliminated, through adjusting the durations of the operating vectors. A new strategy is proposed for zero-sequence voltage elimination, which utilizes unified voltage modulation and a decoupled SVPWM strategy to achieve two same-sized equivalent vectors for an angle of $120^{\circ}$, generated by two inverters independently. Both simulation and experimental results have verified its efficiency in the instantaneous value elimination of zero-sequence voltage.

Yg-Yg 3상 내철형 변압기의 영상분 임피던스 분석 (Zero Sequence Impedance of Yg-Yg Three Phase Core Type Transformer)

  • 조현식;조성우;신창훈;차한주
    • 전기학회논문지
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    • 제65권6호
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    • pp.940-945
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    • 2016
  • In this paper, zero sequence equivalent circuit of Yg-Yg three phase core-type transformer is analyzed. Many problems by iron core structure of the three phase transformer due to asymmetric three phase lines, which includes line disconnection, ground fault, COS OFF, and unbalanced load are reported in the distribution system. To verify a feasibility of zero sequence impedance of Yg-Yg type three phase transformer, fault current generation in the three phase core and shell-type Yg-Yg transformer is compared by PSCAD/EMTDC when single line ground fault is occurred. As a result, shell-type transformer does not affect the flow of fault current, but core-type transformer generate an adverse effect by the zero sequence impedance. The adverse effect is explained by the zero sequence equivalent circuit of core-type transformer and Yg-Yg type three phase core-type transformer supplies a zero sequence fault current to the distribution system.

공정제어에서 선택시퀀스를 위한 효율적인 리모트 콘트롤 제어방법 (An Efficient Method of Remote Control for Select Sequence in Process Control)

  • 공헌택;김치수;유정봉
    • 한국산학기술학회논문지
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    • 제11권1호
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    • pp.107-112
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    • 2010
  • PLC를 사용한 제어시스템에서 SFC 언어를 사용하면 전체 공정의 흐름을 이해하기 쉽고 유지보수가 용이하다. SFC 언어는 단일 시퀀스, 선택 시퀀스, 병렬 시퀀스로 나누어지고, 여러 조건에 맞게 처리하는 선택시퀀스로 프로그램 할 때 선택분기 스텝에 에러가 발생하면 전체 공정이 정지하게 된다. 이 에러스텝이 전체 공정에 크게 영향을 안주는 공정이라면 전체 공정을 정지시키지 않고 에러를 처리하면 손실은 줄어들게 된다. 본 논문에서는 선택시퀀스에서 리모트 콘트롤을 사용하여 선택시퀀스를 효율적으로 제어하는 방법을 제시하고 적용 예를 통해 그의 타당성을 확인하였다.

CAZAC Sequence를 이용한 SC-FDE 시스템 설계 (Design of SC-FDE System Using CAZAC Sequence)

  • 강훈;임세빈;최형진
    • 한국통신학회논문지
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    • 제32권2A호
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    • pp.169-178
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    • 2007
  • 본 논문에서는 SC-FDE(Single Carrier with Frequency Domain Equalization) 시스템의 수신 성능을 최적화하기 위한 신호 구조와 수신기 설계 방안을 제안한다. 기존의 SC-FDE 시스템은 수신단에서 신호 복조를 위해 이용되는 파일럿 신호가 시간 영역에서 생성되기 때문에 주파수 영역 전력 분포가 불균일하여 채널 등화 과정에서의 오류를 발생시키며, 이는 수신기 성능 열화의 주요인으로 작용한다. 본 논문에서는 기존 방식의 단점을 보완하기 위해 시간과 주파수 영역 모두에서 전력 분포가 일정한 CAZAC(Constant Amplitude Zero Auto-Correlation) sequence를 응용하여 이를 반복하는 형태로 파일럿 신호를 생성함으로써 수신 성능을 향상시킬 수 있는 신호 구조를 설계하였으며, 이에 적합한 수신기 구조를 제시한다. 컴퓨터 시뮬레이션을 통해 이동 무선 채널 환경에서 제안된 구조가 기존의 구조에 비해 프레임과 주파수 동기 그리고 채널 등화의 관점에서 더욱 우수한 성능을 보임을 입증하였다.

한탄바이러스 호왕주의 L 유전자 절편의 염기서열 (Molecular Characterization of the L Segment of Hantaan Virus, Strain Howang)

  • 주용규;송대용;구형모;이호왕
    • 대한바이러스학회지
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    • 제29권3호
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    • pp.155-163
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    • 1999
  • Hantaan virus (HTNV), the etiologic agent of hemorrhagic fever with renal syndrome (HFRS), belongs to the genus Hantavirus, and has three single negative stranded RNA genome segments. HTNV strain Howang isolated from the blood of severe case of Korean HFRS is more virulent than HTNV 76/118 and the M and S genome segments' nucleotide sequence of Howang strain showed 93.5% and 94% homology to each segment of HTNV 76/118. We have obtained 6533 nucleotides long sequence of the L genome segment of Howang strain using reverse transcriptase in conjunction with PCR amplification and compared to other hantaviruses. The messenger sense of the L segment contains one long single long open reading frame of 2151 amino acids, which encodes a deduced RNA dependent RNA polymerase of 246.4 kDa caculated molecular weight protein. The nucleotide sequence of the L segment of Howang strain shows 93%, 74%, 66%, 65% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively. The amino acid sequence of the L segment of Howang strain shows 99%, 85%, 68%, 68% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively.

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Single Nucleotide Polymorphism Marker Discovery from Transcriptome Sequencing for Marker-assisted Backcrossing in Capsicum

  • Kang, Jin-Ho;Yang, Hee-Bum;Jeong, Hyeon-Seok;Choe, Phillip;Kwon, Jin-Kyung;Kang, Byoung-Cheorl
    • 원예과학기술지
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    • 제32권4호
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    • pp.535-543
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    • 2014
  • Backcross breeding is the method most commonly used to introgress new traits into elite lines. Conventional backcross breeding requires at least 4-5 generations to recover the genomic background of the recurrent parent. Marker-assisted backcrossing (MABC) represents a new breeding approach that can substantially reduce breeding time and cost. For successful MABC, highly polymorphic markers with known positions in each chromosome are essential. Single nucleotide polymorphism (SNP) markers have many advantages over other marker systems for MABC due to their high abundance and amenability to genotyping automation. To facilitate MABC in hot pepper (Capsicum annuum), we utilized expressed sequence tags (ESTs) to develop SNP markers in this study. For SNP identification, we used Bukang $F_1$-hybrid pepper ESTs to prepare a reference sequence through de novo assembly. We performed large-scale transcriptome sequencing of eight accessions using the Illumina Genome Analyzer (IGA) IIx platform by Solexa, which generated small sequence fragments of about 90-100 bp. By aligning each contig to the reference sequence, 58,151 SNPs were identified. After filtering for polymorphism, segregation ratio, and lack of proximity to other SNPS or exon/intron boundaries, a total of 1,910 putative SNPs were chosen and positioned to a pepper linkage map. We further selected 412 SNPs evenly distributed on each chromosome and primers were designed for high throughput SNP assays and tested using a genetic diversity panel of 27 Capsicum accessions. The SNP markers clearly distinguished each accession. These results suggest that the SNP marker set developed in this study will be valuable for MABC, genetic mapping, and comparative genome analysis.

Seismic performance assessments of precast energy dissipation shear wall structures under earthquake sequence excitations

  • Zhang, Hao;Li, Chao;Wang, Zhi-Fang;Zhang, Cai-Yan
    • Earthquakes and Structures
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    • 제18권2호
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    • pp.147-162
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    • 2020
  • This paper presents a novel precast energy dissipation shear wall (PEDSW) structure system that using mild steel dampers as dry connectors at the vertical joints to connect adjacent wall panels. Analytical studies are systematically conducted to investigate the seismic performance of the proposed PEDSW under sequence-type ground motions. During earthquake events, earthquake sequences have the potential to cause severe damage to structures and threaten life safety. To date, the damage probability of engineering structures under earthquake sequence has not been included in structural design codes. In this study, numerical simulations on single-story PEDSW are carried out to validate the feasibility and reliability of using mild steel dampers to connect the precast shear walls. The seismic responses of the PEDSW and cast-in-place shear wall (CIPSW) are comparatively studied based on nonlinear time-history analyses, and the effectiveness of the proposed high-rise PEDSW is demonstrated. Next, the foreshock-mainshock-aftershock type earthquake sequences are constructed, and the seismic response and fragility curves of the PEDSW under single mainshock and earthquake sequences are analyzed and compared. Finally, the fragility analysis of PEDSW structure under earthquake sequences is performed. The influences of scaling factor of the aftershocks (foreshocks) to the mainshocks on the fragility of the PEDSW structure under different damage states are investigated. The numerical results reveal that neglecting the effect of earthquake sequence can lead to underestimated seismic responses and fragilities, which may result in unsafe design schemes of PEDSW structures.

Distance Measurement Using a Single Camera with a Rotating Mirror

  • Kim Hyongsuk;Lin Chun-Shin;Song Jaehong;Chae Heesung
    • International Journal of Control, Automation, and Systems
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    • 제3권4호
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    • pp.542-551
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    • 2005
  • A new distance measurement method with the use of a single camera and a rotating mirror is presented. A camera in front of a rotating mirror acquires a sequence of reflected images, from which distance information is extracted. The distance measurement is based on the idea that the corresponding pixel of an object point at a longer distance moves at a higher speed in a sequence of images in this type of system setting. Distance measurement based on such pixel movement is investigated. Like many other image-based techniques, this presented technique requires matching corresponding points in two images. To alleviate such difficulty, two kinds of techniques of image tracking through the sequence of images and the utilization of multiple sets of image frames are described. Precision improvement is possible and is one attractive merit. The presented approach with a rotating mirror is especially suitable for such multiple measurements. The imprecision caused by the physical limit could be improved through making several measurements and taking an average. In this paper, mathematics necessary for implementing the technique is derived and presented. Also, the error sensitivities of related parameters are analyzed. Experimental results using the real camera-mirror setup are reported.

매엽식 세정장비의 동작순서 시뮬레이션 및 웨이퍼 처리량 측정에 관한 연구 (Study on Measurement of Wafer Processing Throughput and Sequence Simulation of SWP(Single Wafer Process) Cleaning Equipment)

  • 선복근;한광록
    • 전자공학회논문지CI
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    • 제42권5호
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    • pp.31-40
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    • 2005
  • 본 연구에서는 웨이퍼의 식각, 세정, 연마 공정에 사용되는 매엽식 세정장비의 동작순서의 시뮬레이션과 단위 시간당 처리량 측정 방법에 대해 연구한다. 유한상태기계를 바탕으로 스케쥴링 알고리즘에 따른 로봇의 상태를 정의하여 시뮬레이션 모델을 구축하였으며, 이에 따른 시뮬레이션 수행을 통해 세정장비의 시간당 처리량을 측정하였다. 본 연구에서 제시한 시뮬레이션 기법을 통해 레시피와 로봇의 동작속도에 따라 세정장비의 단위시간당 처리량을 측정하고, 처리량을 극대화 할 수 있는 레시피와 로봇의 동작순서를 찾아낼 수 있다.