• Title/Summary/Keyword: Single root

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Formation of Teleomorph of the White Root Rot Fungus, Rosellinia necatrix, and the Potential Role of its Ascospores as Inocula

  • Lee, J.S.;Han, K.S.;Park, J.H.;Park, Y.M.;Naoyuki, Matsumoto
    • The Plant Pathology Journal
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    • v.19 no.3
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    • pp.152-158
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    • 2003
  • Stromata of the white root rot fungus, Rosellinia necatrix, were produced on diseased roots although they were reported to develop rarely in nature. Forty-two (42) out of 47 samples produced synnemata while 23 developed stromata. Forty-seven (47) isolates obtained from diseased root samples were divided into 24 mycelium compatibility groups (MCGs). Sixteen (16) out of 24 MCGs produced stromata. Single ascospore isolates from 10 stroma samples produced dsRNA-containing isolates from diseased tissue beneath stromata. The frequency of synnema production on axenic culture varied among isolates with different origin. The dsRNA was not transmitted vertically to the ascospore offspring despite the infection of various dsRNA in the parental isolates. The dsRNA was absent in 35 ascospore isolates in two stroma samples that originated from the isolates, in which dsRNA was not eliminated by hyphal tip isolation. Consequently, sexual reproduction in the white root rot fungus was suggested to produce propagules as a new infection source and to have the function to eliminate infectious factors such as mycoviruses.

Washout resistance of fast-setting pozzolan cement under various root canal irrigants

  • Jang, Ga-Yeon;Park, Su-Jung;Heo, Seok-Mo;Yu, Mi-Kyung;Lee, Kwang-Won;Min, Kyung-San
    • Restorative Dentistry and Endodontics
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    • v.38 no.4
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    • pp.248-252
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    • 2013
  • Objectives: Fast-setting pozzolan cement (Endocem, Maruchi) was recently developed. The aim of this study was to investigate the effects of various root canal irrigants on the washout of Endocem in comparison to the previously marketed mineral trioxide aggregate (ProRoot; Dentsply) in a furcal perforation model. Materials and Methods: ProRoot and Endocem were placed into acrylic molds on moist Oasis. Each mold was then immediately exposed to either physiologic saline, 2.5% sodium hypochlorite (NaOCl), or 2% chlorhexidine (CHX) under gentle shaking for five minutes. Washout testing was performed by scoring scanning electron microscope (SEM) images. Results: Endocem exhibited higher washout resistance compared to ProRoot, especially in the NaOCl group. Conclusions: These results suggest that Endocem can be considered a useful repair material for furcal perforation, especially in a single-visit scenario.

Maxillary first molar with 7 root canals diagnosed using cone-beam computed tomography

  • Rodrigues, Evaldo;Braitt, Antonio Henrique;Galvao, Bruno Ferraz;da Silva, Emmanuel Joao Nogueira Leal
    • Restorative Dentistry and Endodontics
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    • v.42 no.1
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    • pp.60-64
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    • 2017
  • Root canal anatomy is complex, and the recognition of anatomic variations could be a challenge for clinicians. This case report describes the importance of cone beam computed tomographyic (CBCT) imaging during endodontic treatment. A 23 year old woman was referred by her general dental practitioner with the chief complaint of spontaneous pain in her right posterior maxilla. From the clinical and radiographic findings, a diagnosis of symptomatic irreversible pulpitis was made and endodontic treatment was suggested to the patient. The patient underwent CBCT examination, and CBCT scan slices revealed seven canals: three mesiobuccal (MB1, MB2, and MB3), two distobuccal (DB1 and DB2), and two palatal (P1 and P2). Canals were successfully treated with reciprocating files and filled using single-cone filling technique. Precise knowledge of root canal morphology and its variation is important during root canal treatment. CBCT examination is an excellent tool for identifying and managing these complex root canal systems.

Nerve Conduction Velocity through the Ventral Root Afferent Fibers in the Cat (고양이 척수전근 감각신경섬유의 흥분전도속도)

  • Kim, Jun;Hwang, Sang-Ik;Ho, Won-Kyung
    • The Korean Journal of Physiology
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    • v.21 no.1
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    • pp.59-66
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    • 1987
  • This study was aimed to investigate whether the conduction velocity of nerve impulses through the ventral afferent fibers is constant along their entire courses in dorsal as well as in ventral roots. Cats were anesthetized with ${\alpha}-chloralose$ (60 mg/kg, i.p.) and artificially ventilated. Laminectomies were done on L4-S1 spinal vertebrae to expose the lumbosacral spiral cord. Both ventral and dorsal roots of L7 or S1 spinal segments were isolated and cut near the spinal cord. Ventral roots were placed on 6-lead stimulating electrodes and stimulated with supra C-threshold intensity. Divided dorsal root fascicles were placed on bipolar recording electrodes and single fiber units activated by the stimulation of the ventral roots were identified. Followings are the results obtained: 1) A total of 27 VRA units were identified. 10 units of them conducted impulses slower than 2 m/sec. Conduction velocities of the remaining units were in the range of 3.11-20.91 m/sec. 2) In 12 Units conduction velocities Of the VRA units through dorsal$(CV_{DR})$ and venral root$(CV_{DR})$ were determined respectively. There was a tendency to conduct impulses faster through dorsal roots$(CV_{DR}=8.19{\pm}3.26\;m/sec)$ than ventral roots$(CV_{DR}=3.46{\pm}1.02\;m/sec)$. From the above results we confirmed that there exist nerve fibers in continuity between the spinal ventral and dorsal roots but we could not ascertain whether there is a change in conduction velocity through the entire course of ventral afferent unit.

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Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

  • Gurgel-Filho, Eduardo Diogo;Lima, Felipe Coelho;Saboia, Vicente De Paula Aragao;Coutinho-Filho, Tauby De Souza;Neves, Aline De Almeida;da Silva, Emmanuel Joao Nogueira Leal
    • Restorative Dentistry and Endodontics
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    • v.39 no.4
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    • pp.282-287
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    • 2014
  • Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta-Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower push-out bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.

Push-out bond strength and marginal adaptation of apical plugs with bioactive endodontic cements in simulated immature teeth

  • Maria Aparecida Barbosa de Sa;Eduardo Nunes ;Alberto Nogueira da Gama Antunes ;Manoel Brito Junior ;Martinho Campolina Rebello Horta ;Rodrigo Rodrigues Amaral;Stephen Cohen ;Frank Ferreira Silveira
    • Restorative Dentistry and Endodontics
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    • v.46 no.4
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    • pp.53.1-53.11
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    • 2021
  • Objectives: This study evaluates the bond strength and marginal adaptation of mineral trioxide aggregate (MTA) Repair HP and Biodentine used as apical plugs; MTA was used as reference material for comparison. Materials and Methods: A total of 30 single-rooted teeth with standardized, artificially created open apices were randomly divided into 3 groups (n = 10 per group), according to the material used to form 6-mm-thick apical plugs: group 1 (MTA Repair HP); group 2 (Biodentine); and group 3 (white MTA). Subsequently, the specimens were transversely sectioned to obtain 2 (cervical and apical) 2.5-mm-thick slices per root. Epoxy resin replicas were observed under a scanning electron microscope to measure the gap size at the material/dentin interface (the largest and smaller gaps were recorded for each replica). The bond strength of the investigated materials to dentin was determined using the push-out test. The variable bond strengths and gap sizes were evaluated independently at the apical and cervical root dentin slices. Data were analyzed using descriptive and analytic statistics. Results: The comparison between the groups regarding the variables' bond strengths and gap sizes showed no statistical difference (p > 0.05) except for a single difference in the smallest gap at the cervical root dentin slice, which was higher in group 3 than in group 1 (p < 0.05). Conclusions: The bond strength and marginal adaptation to root canal walls of MTA HP and Biodentine cement were comparable to white MTA.

Effect of Mineral Nutrient Control on Nutrient Uptake, Growth and Yield of Single-Node Cutting Rose Grown in a Closed Hydroponic System (순환식 수경재배시 무기이온 조절이 Single-Node Cutting 장미의 양분흡수, 생육 및 품질에 미치는 영향)

  • Yang, Eun-Young;Park, Keum-Soon;Oh, Jeong-Sim;Lee, Hye-Jin;Lee, Yong-Beom
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.252-260
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    • 2008
  • This study was conducted to observe the characteristics of mineral nutrient uptake of single-node cutting rose 'Versilla' and to determine optimal nutrient solution control method for soilless culture of 'Versilla' in a closed hydroponic system. Nutrient solution was managed by five different control methods: macro- and micro-element control in aeroponic system (M&M), macroelement control in aeroponic system (M), nutrient solution supplement in aeroponic system (S); electrical conductivity (EC) control in aeroponic system(EC-A); EC control in deep flow technique system(EC-D). The concentration of $NO_3$-N exceeds optimal range whereas P and Mg decreased at the later stage of plant growth with the EC control method, EC-A and EC-D. The overall mineral nutrient content increased with S. On the other hand the nutrient content at the root environment was maintained optimal with M&M and M. The nutrient solution control methods had significantly effect on the cut-flower quality. In the M&M and M, flower length, fresh weight and root activity were higher than those with the other mineral nutrients control methods. The maximal efficiency of photochemistry (Fv/Fm) was higher for M&M, M and S than that with EC-A and EC-D. Based on the above results, it is highly recommended to control nutrient solution by mineral nutrient control methods (M&M and M) in a closed hydroponic system for single-node cutting rose, 'Versillia'.

Comparison of high-resolution and standard zoom imaging modes in cone beam computed tomography for detection of longitudinal root fracture: An in vitro study

  • Taramsari, Mehran;Kajan, Zahra Dalili;Bashirzadeh, Parinaz;Salamat, Fatemeh
    • Imaging Science in Dentistry
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    • v.43 no.3
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    • pp.171-177
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    • 2013
  • Purpose: The purpose of this study was to compare the efficacy of two imaging modes in a cone beam computed tomography (CBCT) system in detecting root fracture in endodontically-treated teeth with fiber posts or screw posts by selecting two fields of view. Materials and Methods: In this study, 78 endodontically-treated single canal premolars were included. A post space was created in all of them. Then the teeth were randomly set in one of 6 artificial dental arches. In 39 of the 78 teeth set in the 6 dental arches, a root fracture was intentionally created. Next, a fiber post and a screw post were cemented into 26 teeth having equal the root fractures. High resolution (HiRes) and standard zoom images were provided by a CBCT device. Upon considering the reconstructed images, two observers in agreement with each other confirmed the presence or absence of root fracture. A McNemar test was used for comparing the results of the two modes. Results: The frequency of making a correct diagnosis using the HiRes zoom imaging mode was 71.8% and in standard zoom was 59%. The overall sensitivity and specificity in diagnosing root fracture in the HiRes mode were 71.79% and 46.15% and in the standard zoom modes were 58.97% and 33.33%, respectively. Conclusion: There were no significant differences between the diagnostic values of the two imaging modes used in the diagnosis of root fracture or in the presence of root canal restorations. In both modes, the most true-positive results were reported in the post space group.

Design of Square Root and Inverse Square Root Arithmetic Units for Mobile 3D Graphic Processing (모바일 3차원 그래픽 연산을 위한 제곱근 및 역제곱근 연산기 구조 및 설계)

  • Lee, Chan-Ho
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.3
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    • pp.20-25
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    • 2009
  • We propose hardware architecture of floating-point square root and inverse square root arithmetic units using lookup tables. They are used for lighting engines and shader processor for 3D graphic processing. The architecture is based on Taylor series expansion and consists of lookup tables and correction units so that the size of look-up tables are reduced. It can be applied to 32 bit floating point formats of IEEE-754 and reduced 24 bit floating point formats. The square root and inverse square root arithmetic units for 32 bit and 24 bit floating format number are designed as the proposed architecture. They can operation in a single cycle, and satisfy the precision of $10^{-5}$ required by OpenGL 1.x ES. They are designed using Verilog-HDL and the RTL codes are verified using an FPGA.

Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy

  • Eren, Selen Kucukkaya;Uzunoglu, Emel;Sezer, Banu;Yilmaz, Zeliha;Boyaci, Ismail Hakki
    • Restorative Dentistry and Endodontics
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    • v.43 no.1
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    • pp.11.1-11.10
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    • 2018
  • Objectives: This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin. Materials and Methods: Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically. Results: In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001). Conclusions: Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.