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Comparative Renal Papillary and Pelvic Epithelial Morphology of the Mammalian Kidney (포유동물의 신유두 및 신우상피에 대한 비교형태학적 연구)

  • Kim, Jin;Oh, Su-Ja;Park, In-Sun;Chung, Jin-Woong
    • Applied Microscopy
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    • v.17 no.1
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    • pp.131-160
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    • 1987
  • Anatomical features of the renal papilla and pelvis and ultrastructures of the epithelium covering these areas in four species of mammals were studied by means of light, scanning and transmission electron microscopy. In terms of the morphology of mammalian kidney types distinguished by Sperber(1944), Pfeiffer(1968) and Schmidt-Nielsen(1977), the kidneys of animal species used in this experiment were; 1) the mouse kidney with the fornix between a long conical papilla and the funnel-shaped pelvis, 2) the guinea pig kidney with the peripelvic column and pelvic pouch between a short conical papilla and the funnel-shaped pelvis, 3) the dog kidney with the peripelvic column and pelvic pouch between the crest-shaped papilla and the funnel-shaped pelvis, and 4) the cattle kidney which is divided into multiple renculi with minor and major calyces and pelvis. The renal papilla was lined with the simple or pseudostratified columnar epithelium which covered the inner zone of the renal medulla. The epithelial cells with numerous short microvilli on the surface contained a few organelles. In the mouse, the fornix was lined with one to two cell-layered cuboidal epithelium which covered the outer zone of the renal medulla and a part of the cortex. The epithelial cells of the fornix with numerous short microvilli or microridges on the surface had well-developed organelles. In the guinea pig, the peripelvic column was lined with the simple cuboidal or low columnar epithelium which covered the outer zone of the renal medulla. The epithelial cells with numerous short microvilli on the surface contained well-developed organelles. The pelvic pouch was lined with the pseudostratified columnar epithelium which was composed of four kinds of cells; the secretory cell with small electron-dense granules (310 nm), the secretory cell with large granules (720 nm) showing various electron densities, the mitochondria-rich cell with a single cilium, and the basal cell. Pelves of the mouse and guinea pig, peripelvic column, pelvic pouch and pelvis of the dog, and minor and major calyces and pelvis of the cattle were lined with the transitional epithelium. The fusiform vesicles in the superficial cells of the epithelium were highly developed in the dog, relatively well developed in the mouse and guinea pig, and poorly developed in the cattle. From the above findings, it is suggested that the transport of solutes and water of the urine in the pelvic cavity can take place through the epithelia covering the renal papilla and fornix of the mouse, papilla and peripelvic column of the guinea pig, and papillae of the dog and the cattle. And specialized cell types in the epithelium of the guinea pig pelvic pouch, two kinds of secretory cells and mitochondria-rich cell with a single cilium, could have peculiar functions in the renal pelvis, respectively.

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A Study on the Ancient Architecture in view of the Stone Remains (focused on the 3 Kingdom Period and Unificated Shilla Period) (석조유구(石造遺構)를 통한 한국(韓國) 고대건축(古代建築)에 관한 연구(硏究) -삼국시대와 통일신라시대를 중심으로-)

  • Cheon, Deuk-Youm;Park, Ji-Min
    • Journal of architectural history
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    • v.8 no.3 s.20
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    • pp.23-38
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    • 1999
  • The purpose of this study is to analogize the appearance of Korean Ancient Architecture in view of the Stone Remains from 3 Kingdom Period to Unificated Shilla Period. But in these period, there is no building remains but some stupas and fine arts. Especially, there are many architectural appearance and revealing signature in these Historical Stone remains. Architectural elements which are analogized by stone remains what has value as historical materials by preservation of original form from 3 Kingdom Preiod to Unificated Shilla Period are as follows : 1) Platform, the representative characteristic of Korean traditional architecture, was frame structure and accumulate structure. And circular or square footing stood a same shape column on it is put on the platform. 2) In the case of column, there used entasis column and inclined column and circular chamfer technique was applied on the top side of it. Upper side of column, capital and head pentrating tie that small bearing block was put on the center of it was joined. And longitu야nal rest(長舌) supported a cross beam. Capital and small bearing block had no bottom heel, and heel side was curved and straight. Centered bracket structure was often used, and multi bracket structure is not used yet. Inward incline technique was used. 3) Inward opening pair door which had lintel, threshold, doorjamb was usually used, Fixing stone was used for structural safety, and circular handle and lock was used for decoration. Handrail was used on the edge of wooden floor for decorative effect and safety. 4) Square rafter and circular rafter were used in the same period and so did flying rafter. Double eaves and single eave were used in the same period but, single eave was usually used. In this period, square rafter was usually used. This would be studied more by comparing with Japanese wooden architecture. 5) Hipped roof was used and half-hipped roof was not used yet. In front of th hip, there are small sculpture called Jap-Sang(雜像), and windbell was hang on the end of the hip rafter. Concave roof tile, convex roof tile, round eaver tile, decorative tile at end of roof ridge were used. Lotus style was well used on the face of roof tile for decoration. From the results of this study, wooden architecture of Unificated Shilla period was simple compare to Koryo dynasty and Chosun dynasty but, it had some brilliant character. It was hard work that analogized the form of non-existent wood architecture of Ancient Korean period by restricted stone remains. But, in addition to the results of this study and research of old documentations, more study should be go on.

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Isolation and Identification of Antimicrobial Compound from Amarantus lividus (참비름 추출물에서 항균성 물질의 분리 및 동정)

  • Oh, Young-Sook;Lee, Shin-Ho
    • Microbiology and Biotechnology Letters
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    • v.33 no.2
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    • pp.123-129
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    • 2005
  • Isolation and identification of pathogens from slaughter and meat processing plant were investigated. Antimicrobial activity of Amaranthus lividus against isolated pathogens such as Aeromonas sobria, Escherichia coli, Escherichia coli O157, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus was investigated. Among the chloroform, ethyl acetate and buthanol fraction of amaranthus lividus showed inhibitory effect against Aeromonas sobria CLFM1 and Escherichia coli CLFM2. Antimicrobial substance in chloroform fraction was isolated by silica gel adsorption column chromatography, sephadex LH-20 column chromatography and silica gel partition column chromatography. The antimicrobial compound of amaranthus lividus was identified as diethyl phtalate by HPLC, GC-MS, H-NMR and C-NMR.

Removal of Heavy Metals from Aqueous Solution by a Column Packed with Peat-Humin (Peat-Humin 충전 칼럼을 이용한 수용액 중의 중금속 제거)

  • Shin, Hyun-Snag;Lee, Chang-Hoon;Lee, Yo-Snag;Kang, Ki-Hoon
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.5
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    • pp.535-541
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    • 2005
  • Peat humin(p-Humin) extracted from Canadian Sphagnum peat moss was packed in a column and removal of heavy metal ions such as Cd, Cu and Pb from aqueous solution under flow conditions was studied. The metal ions were removed not only from single-element solutions but also from a multi-metal solution. Column kinetics for metal removal were described by the Thomas model. For single-component metal solutions, the maximum adsorption capacities of the p-Humin for Pb, Cu and Cd were 138.8, 44.66 and 41.61 mg/g, respectively. The results of multi-component competitive adsorption showed that adsorption affinity was in the order of Pb $\gg$ Cu > Cd. The adsorbed metal ions were easily deserted from the p-Humin with 0.05 N $HNO_3$ solution. It is apparent that 95% of the heavy metal ions were recovered from the saturated column. This investigation provides possibility to clean up heavy-metal contaminated waste waters by using the natural biomass, p-Humin as an environmentally friendly and cost-effective new biosorbents.

Member Utilization Concept Design for Hollow Circular Section Multi-column Tower Subjected to 10MW Level Wind Turbines (10MW급 풍력발전용 원형강관 멀티기둥타워의 부재유용도 개념설계)

  • Kim, Kyungsik;Kim, Mi Jin
    • Journal of Korean Society of Steel Construction
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    • v.29 no.3
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    • pp.205-215
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    • 2017
  • This study presents an example of conceptual design for hollow circular section multi-column tower system subjected to 10MW level wind load by introducing a method based on member utilization that examine both structural stability and economical efficiency. The basic assumptions for the proto type of a multi-column tower that can replace a single-cylinder tower were suggested and structural models were constructed following the assumptions and analyzed for identifying member forces. Based on the calculated member strengths and acting loads, the member utilization of the proposed multi-column tower structures were calculated for axial force, shear, bending and torsion and evaluaed for suitability as a wind tower. Design parameters such as steel tube dimensions, slenderness ratio, and number of floors for braces was proposed in the acceptable range of member utilization for conceptual design of multi-column wind towers.

A Growth-Stimulating Protein in Cow's Milk

  • Ki, Eun-Kyung;Park, Yong-Bok;Choi, Myung-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.4 no.3
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    • pp.59-65
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    • 1994
  • Growth-stimulating effects of cow's milk was examined using Vero cell cultre. Medium containing whole cow's milk stimulated cell growth about the same degree as that containing fetal bovine serum. The growth-stimulating factor in cow's milk was purified using hydrophobic (phenyl-sepharose) and gel filtration (Sephadex G-100) column chromatographies. It appeared that the factor is a highly hydrophobic protein, since the major growth-stimulating activity was found in the fractions eluted with 50% ethylene glycol from the phenyl-sepharose column during the purification. The purified factor showed a single band on the polyacrylamide gel electrophoresis in the presence of 1% (w/v) SDS. The factor has been found to have a relatively high molecular weight in the range of about Mr=100,000-150,000. In the presence of the purified factor (5%, w/v) in the culture medium, the incorporation of [3H]-thymidine into the cells was increased approximately 2,400-fold over that in the presence of 5% (w/v) fetal bovine serum. It seems that the growth-stimulating factor purified in this study is one of the major growth factors in the cow's milk.

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Spray Characteristics of Liquid Jets in Acoustically-Forced Crossflows (음향가진된 횡단류 유동장 내 액체제트의 분무특성)

  • Song, Yoonho;Hwang, Donghyun;Ahn, Kyubok
    • Journal of the Korean Society of Propulsion Engineers
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    • v.22 no.2
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    • pp.1-10
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    • 2018
  • This study investigated the acoustic forcing effects on the liquid column breakup length and the trajectory of liquid jets in crossflows. Cold-flow tests with a single hole circular nozzle injector were carried out by changing the injection pressure and acoustic forcing amplitude. Additionally, spray images were obtained at 12 phase angles to investigate the influence of the phage angle. The results revealed that the liquid column breakup lengths generally decreased under the acoustic forcing conditions, in comparison to those under the non-acoustic forcing conditions. However, they were not affected by the variation in the phase angles. On the contrary, it was found that the acoustic forcing hardly influenced the liquid column trajectories.

Collapse resistance of steel frames in two-side-column-removal scenario: Analytical method and design approach

  • Zhang, JingZhou;Yam, Michael C.H.;Soltanieh, Ghazaleh;Feng, Ran
    • Structural Engineering and Mechanics
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    • v.78 no.4
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    • pp.485-496
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    • 2021
  • So far analytical methods on collapse assessment of three-dimensional (3-D) steel frames have mainly focused on a single-column-removal scenario. However, the collapse of the Federal Building in the US due to car bomb explosion indicated that the loss of multiple columns may occur in the real structures, wherein the structures are more vulnerable to collapse. Meanwhile, the General Services Administration (GSA) in the US suggested that the removal of side columns of the structure has a great possibility to cause collapse. Therefore, this paper analytically deals with the robustness of 3-D steel frames in a two-side-column-removal (TSCR) scenario. Analytical method is first proposed to determine the collapse resistance of the frame during this column-removal procedure. The reliability of the analytical method is verified by the finite element results. Moreover, a design-based methodology is proposed to quickly assess the robustness of the frame due to a TSCR scenario. It is found the analytical method can reasonably predict the resistance-displacement relationship of the frame in the TSCR scenario, with an error generally less than 10%. The parametric numerical analyses suggest that the slab thickness mainly affects the plastic bearing capacity of the frame. The rebar diameter mainly affects the capacity of the frame at large displacement. However, the steel beam section height affects both the plastic and ultimate bearing capacity of the frame. A case study on a six-storey steel frame shows that the design-based methodology provides a conservative prediction on the robustness of the frame.

A 8-bit Variable Gain Single-slope ADC for CMOS Image Sensor

  • Park, Soo-Yang;Son, Sang-Hee;Chung, Won-Sup
    • Journal of IKEEE
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    • v.11 no.1 s.20
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    • pp.38-45
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    • 2007
  • A new 8-bit single-slope ADC using analog RAMP generator with digitally controllable dynamic range has been proposed and simulated for column level or per-pixel CMOS image sensor application. The conversion gain of ADC can he controlled easily by using frequency divider with digitally controllable diviber ratio, coarse/fine RAMP with class-AB op-amp, resistor strings, decoder, comparator, and etc. The chip area and power consumption can be decreased by simplified analog circuits and passive components. Proposed frequency divider has been implemented and verified with 0.65um, 2-poly, 2-metal standard CMOS process. And the functional verification has been simulated and accomplished in a 0.35$\mu$m standard CMOS process.

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Investigation of natural frequencies of multi-bay and multi-storey frames using a single variable shear deformation theory

  • Bozyigit, Baran;Yesilce, Yusuf
    • Structural Engineering and Mechanics
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    • v.65 no.1
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    • pp.9-17
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    • 2018
  • This study concerns about calculating exact natural frequencies of frames using a single variable shear deformation theory (SVSDT) which considers the parabolic shear stress distribution across the cross section. Free vibration analyses are performed for multi-bay, multi-storey and multi-bay multi-storey type frame structures. Dynamic stiffness formulations are derived and used to obtain first five natural frequencies of frames. Different beam and column cross sections are considered to reveal their effects on free vibration analysis. The calculated natural frequencies are tabulated with the results obtained using Euler-Bernoulli Beam Theory (EBT) and Timoshenko Beam Theory (TBT). Moreover, the effects of inner and outer columns on natural frequencies are compared for multi-bay frames. Several mode shapes are plotted.