• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1378-1385
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• 2010

A novel agarolytic bacterium, KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of Gram-staining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified as Cellvibrio sp. By ammonium sulfate precipitation followed by Toyopearl QAE-550C, Toyopearl HW-55F, and MonoQ column chromatographies, the extracellular agarase in the culture fluid could be purified 120.2-fold with a yield of 8.1%. The specific activity of the purified agarase was 84.2 U/mg. The molecular mass of the purified agarase was 70 kDa as determined by dodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal temperature and pH of the purified agarase were $35^{\circ}C$ and pH 7.0, respectively. The purified agarase failed to hydrolyze the other polysaccharide substrates, including carboxymethyl-cellulose, dextran, soluble starch, pectin, and polygalacturonic acid. Kinetic analysis of the agarose hydrolysis catalyzed by the purified agarase using thin-layer chromatography showed that the main products were neoagarobiose, neoagarotetraose, and neoagarohexaose. These results demonstrated that the newly isolated freshwater agarolytic bacterium KY-YJ-3 was a Cellvibrio sp., and could produce an extracellular ${\beta}$-agarase, which hydrolyzed agarose to yield neoagarobiose, neoagarotetraose, and neoagarohexaose as the main products.

• Journal of Environmental Health Sciences
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• v.9 no.1
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• pp.31-47
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• 1983

This study was performed to investigate how much chemical constituents are contained and to what extent they are spread in the major streams running through Taegu City, i. e.: Yeechun, Beomeo, Chilsung, Dalseo, during the seven months Period between March to September, 1982. Nine constituents, pH, DO, COD, T-N, $NH_4-N, NO_2-N, NO_3-N, Cl^-, SO_4--$ and $PO_4-P---$, were found to be-contained in those streams. The results of the survey are summarized as follows. 1) Each chemical constituent detected in the five streams has the following variation range. pH(7.0-9.10), DO (ND-7.46ppm), COD (5.4-173ppm), T-N(13-42ppm), $NH^+_$-N$ (10.2-32.2ppm), $NO^-_2-N$ (0.007-2.53ppm), $NO^-_3-N$ (0.005-2.16ppm), $Cl^-$ (150-469 ppm), $SO_4^{3-}$ (71-1000ppm), $PO_4^{3-}-P$ (0.9-53ppm) The amount of all the constituents except pH, and $NO_3-N$, exceeded the standard value allowed for drinking, farming, and industrial water. 2) The monthly variation in the amount of each constituent differs according to the station from which it was sampled, but in general, the amount increased during the season from March to July when the rainfall was little while it decreased in August when the rainfall was abundant. 3) A statistical analysis of the constituents shows positive correlations that T-N and $NH^+_4-N$, water temperature and $NO^-_3-N$, but there were no ones beween the COD and $NH^+_4-N$ 4 ) The degree of COD pollution in each stream is in the following order. Kongdan, Yeechun, Beomeo, ChiLsung, and Dalseo. 5) Five major streams in Taegu City, Yeechun, Beomeo, Chilsung Dalseo, and Kongdan, are so narrow in width and so short in length that they flow into the Sincheon river or the Gumho river without undergoing self-purification. The increase in population and various kinds of industrial pollutants make the supply insufficient and water pollution inevitable. 4 ) The degree of COD pollution in each stream is in the following order. Kongdan, Yeechun, Beomeo, ChiLsung, and Dalseo.

• Journal of the Korea Institute of Military Science and Technology
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• v.15 no.6
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• pp.721-728
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• 2012

The tracked SUGVs(Small Unmanned Ground Vehicles) are frequently operated in the narrow slope such as stairs and trails. But due to the nature of the tracked vehicle which is steered using friction between the track and the ground and the limited field of view of driving cameras mounted on the lower position, it is not easy for SUGVs to trace narrow slopes. To properly trace inclined narrows, it is very important for SUGVs to keep it's heading direction to the slope. As a matter of factor, no roll value control of a SUGV can makes it's heading being located in the direction of the slope in general terrains. But, the problem is that we cannot directly control roll motion for SUGV. Instead we can control yaw motion. In this paper, a new slope driving method that enables the vehicle trace the narrow slopes with IMU sensor usually mounted in the SUGV is suggested which including an estimation technique of the desired yaw angle corresponding to zero roll angle. In addition, a fuzzy steering controller robust to changes in driving speed and the stair geometry is designed to simulate narrow slope driving with the suggested method. It is shown that the suggested method is quite effective through the simulation.

• Analytical Science and Technology
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• v.32 no.2
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• pp.65-76
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• 2019

Despite the expansion of sewage treatment facilities to reduce pollutants in the tributaries of the Han River, water pollution accidents such as fish deaths continue to frequently occur. The purpose of this study was to identify the pollutant sources using water quality and stable isotope ratio (${\delta}^{15}N$, ${\delta}^{13}C$, ${\delta}^{15}N-NH_4$, ${\delta}^{15}N-NO_3$) analysis results in the three inflow tributaries (Gulpocheon (GP), Anyangcheon (AY) and Sincheon (SC)) of the Han River. Water quality was analyzed in June and October from 2013 to 2017, and the results showed that the concentrations of nutrients, such as T-N, $NO_3-N$, and T-P, were increased at GP4, AY3, SC3, and SC4, which lie downstream of sewage treatment facilities. The results of ${\delta}^{15}N$ for June 2017 indicated that the source of nitrogen was sewage or livestock excreta at GP4 and SC4, and organic fertilizers at AY3 and SC3. ${\delta}^{15}N-NO_3$ results suggested that the source of nitrogen was related to organic sewage, livestock or manure at GP4, AY3 and SC4. Therefore, GP4 and SC4 were more influenced by effluent from sewage treatment facilities than by their tributaries, AY3 and SC3 were considered to be influenced more by their tributary than effluent from sewage treatment facilities. With the results of this study, the source of contamination (sewage treatment facility effluent) of river inflow downstream of Han River could be confirmed using water quality and stable isotope ratio.

• Journal of the Economic Geographical Society of Korea
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• v.23 no.4
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• pp.438-453
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• 2020

This study attempts to examine the process of place-making, to define the role and interrelationship of the subjects and to analyze the placeness of the 'Kim Gwang-seok Road', a representative example of artificial place-making. Through a 10-year urban regeneration project based on the concept of Kim Gwang-seok, the alleyway between Bangcheon Market and the retaining wall of Sincheon-daero has been embedded as a memorial space for Kim Gwang-seok and an cultural art space. However, the existing placeness has weakened as the result of the excessive tourism in the late 2010s, while the characteristics of cultural commercial space has strengthened. This change in place has prompted community disintegration, which has caused the loss of momentum for sustainable development. To overcome these problems, it will be necessary to establish endogenous governance to expand and reproduce existing community capabilities, embedded social capital and place assets in new directions.

• Journal of The Korean Association of Information Education
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• v.24 no.6
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• pp.519-528
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• 2020

Due to COVID-19, most schools are conducting online learning. In this study, a total of 12 block-type educational programming tool classes were conducted in the form of online learning for 6th grade elementary school students, and then quantitatively and qualitatively analyzed students' perceptions of their learning experience on entry learning. As a result of the analysis, it was found that the learner easily recognized the use of the entry program and the difficulty of learning contents, and the learning satisfaction was high. When students face difficulties, it was found that they received the most help from the hint function provided by the site itself, and they were found to be less aware of the necessity of teachers when learning entry. As a result of the qualitative analysis, it was found that the learner felt a lot of novelty and fun through easy and simple operation. On the other hand, it was found that the learning contents and hints were not understood, which made them difficult and felt that the hint contents were insufficient. It was found that students felt a sense of accomplishment by creating and manipulating programs as they wish.

• Journal of Creative Information Culture
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• v.6 no.1
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• pp.49-56
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• 2020

The purpose of this study is to examine overseas educational informatization infrastructure evaluation indicators and create data for establishing and developing educational informatization policy directions in the future. We analyzed the cases of OECD PISA, UNESCO, ITU, the German Wetelsmann Foundation, the U.S. Department of Education, and China as educational indicators. As a result of the analysis, evaluation items for electrical and telecommunication facilities such as the school's Internet connection network were commonly found in all indicators, and the ratio of learners to PCs (LCR) was also found in 3 out of 4 indicators. Through this study, it is expected that it will be of great help in conducting educational informatization evaluation suitable for the rapidly changing modern society and developing evaluation tools.

• Journal of Creative Information Culture
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• v.5 no.1
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• pp.63-70
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• 2019

In this paper, we analyzed 2015 revised curriculum for elementary school's practical art and middle school's information subject based on core competency. As a result, in 2015 revised curriculum for practical art subject, the ability to use information was well reflected in all achievement criteria and learning objectives. Also, problem solving ability and creativity·convergence ability were well reflected. In 2015 revised curriculum for information subject, the ability to use information was well reflected in all achievement criteria and learning objectives as like practical art subject. However, there were fewer learning elements to develop self-management ability. Therefore, it is proposed in this paper that the learning elements and teaching, learning activities and evaluation contents should be included in the SW curriculum, which can further enhance cooperative capabilities, self-management ability and communication ability.

• Journal of the Korean Society of Groundwater Environment
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• v.1 no.1
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• pp.51-58
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• 1994

Electrical soundings and mappings were conducted to elucidate the geoelectrical structures of the local area of Cheju Island. The areas of the electrical surveys are coastal areas of Cheju Island, Gwagji, Sinpung-Sincheon. Generally, the geoelectrical structures of these areas are closely related with hydrogeological structures and it is very important to elucidate the geoelectrical structures for the water supply problems. Even though the results of electrical surveys in coastal areas of Cheju Island differ from place to place, there exists a general tendency of resistivity decrease with depth and the zone in the vicinity of mean sea level has low resistivity value. Also, there is good correlation between the low resistivity zone and the ground water distribution in the survey areas. A careful examination of the results of this study enabled resistivities of the rocks in Cheju Island to be divided in some categories. The resistivities of rocks unsaturated with ground water are greater than about 1000 ohm-m. The rocks saturated with fresh water have the resistivity value of about few hundreds ohm-m. Last, the rocks saturated with saline and/or brackish water have the resistivity value of about few tens ohm-m. The subsurface resistivity distribution of Cheju Island seems to be strongly dependent on the hydraulic characteristics of the rocks, and the amount and the salinity of the ground water in the rocks rather than the surface geology of the area.

• Korean Journal of Environmental Agriculture
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• v.5 no.1
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• pp.30-34
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• 1986

This study was carried out to determine the heavy metal contents in sluges of Keumho river and her branches. The heavy metal contents of sludge are the highest in Sincheon, Kongdancheon and Dalseocheon among the branches. The large part of heavy metal exists in $0{\sim}5cm$ from sludge surface. The sequential extraction with various reagents showed that the residual and organically bound fraction were the most abundant pool. Decomposition of organic matter caused sludge to release heavy metals. On the extraction of sludge with various solution having different pH, it was found that the lower the pH, the more heavy metal was extracted. However, considerable amount of heavy metal was not extracted even with pH 3 solution.