• Natural Product Sciences
• /
• 제22권1호
• /
• pp.64-69
• /
• 2016

A method for simultaneously identifying antioxidative compounds was developed using time-based LC-MS coupled with DPPH assay regardless of the time consuming process. The methanolic extract of Polygonum aviculare (Polygonaceae) showed significant DPPH radical scavenging activity. Time-based DPPH assay for simultaneous identification of active compounds from the extracts of P. aviculare was used. Major peaks of ethyl acetate fraction of P. aviculare showed high DPPH radical scavenging activity. A simple phenolic compound (1) and six flavonoids (2-7) were isolated from the ethyl acetate fraction of P. aviculare by silica gel and sephadex LH-20 column chromatography. The structures of seven compounds were determined to be protocatechuic acid (1), catechin (2), myricitrin (3), epicatechin-3-O-gallate (4), avicularin (5), quercitrin (6), and juglanin (7) based on the analysis of the $^1H$-NMR, $^{13}C$-NMR and ESI-MS data. All compounds exhibited significant antioxidant activity on DPPH assay and active compounds were well correlated with predicted one.

• 식물병연구
• /
• 제26권4호
• /
• pp.264-271
• /
• 2020

이번 연구는 국화에서 문제되는 총채벌레의 종 동정 및 보독 바이러스인 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV)를 동시에 확인할 수 있는 진단방법을 개발하였다. 이는 총채벌레 1마리에서 추출한 핵산에 꽃노랑총채벌레 및 대만총채벌레의 ITS2 부분에 특이적인 프라이머와 TSWV 외피단백질(N) 유전자 특이적인 프라이머를 동시에 넣어 reverse transcription-polymerase chain reaction을 수행하여 DNA를 증폭시키는 방법으로 전기영동하여 각각 287, 367, 777 bp의 DNA 단편의 크기를 비교함으로써 총채벌레의 종 동정 및 총채벌레의 TSWV 보독 여부를 동시에 확인할 수 있다. 충청남도 태안 및 경상남도 창원의 국화 시설하우스에서 총채벌레를 포집하여 총채벌레 우점종과 총채벌레의 TSWV 보독율을 조사한 결과, 태안의 국화 시설하우스에서는 꽃노랑총채벌레가 83.7%로 우점하고 있으며 채집된 총채벌레 중 72.9%가 TSWV를 보독하고 있었으며, 창원에서는 꽃노랑총채벌레가 92.2%를 차지하고 있으며 84.0%의 총채벌레에서 TSWV가 진단되었다. 이러한 결과는 Frankliniella occidentalis가 우점종이며 온실의 국화 식물에서 TSWV의 전반에 중요한 역할을 한다는 것을 확인해준다. 이번 연구는 국화 시설하우스에서 총채벌레를 통한 TSWV의 시설하우스내 유입시기 및 확산 경로 등 바이러스의 역학연구를 위한 간편진단법으로 활용 가능함을 예시해준다.

• 한국식품영양과학회지
• /
• 제26권2호
• /
• pp.209-213
• /
• 1997

민간요법에서 각종 질병치료에 사용되고 있는 어성초의 항균활성 물질을 규명하기 위한 목적으로 연속증류추출장치를 이용하여 포집한 휘발성 정유를 GC-MS로 각 성분을 분리 동정했다. 그 결과 어성초의 휘발성 정유는 GC column에서 52종의 화합물로 분리되었으며, 이중 44종의 화합물이 GC-MS에서 동정되었다. 밝혀진 화합물들은 terpene류 25종, aldehyde류 7종, alcohol류 4종, ketone류 3종 및 기타 5종이며, 이들 중 주요 화합물은 ${\beta}-myrcene$, ${\beta}-ocimene$, decanal, 2-undecanone 및 geranyl propionate이었다.

• Journal of Ginseng Research
• /
• 제34권1호
• /
• pp.41-46
• /
• 2010

Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

• 산업경영시스템학회지
• /
• 제37권2호
• /
• pp.62-69
• /
• 2014

The capability and cost effectiveness of UUV and USV bring to underwater survey, target detection and identification operations have been widely demonstrated and accepted in recent years. Future USV systems may deploy UUVs to gain the advantage of higher area coverage rates through multiple and simultaneous operations. In this paper, we present an architecture of USV and UUV for mine countermeasure with results of measures on effectiveness.

• Journal of Microbiology and Biotechnology
• /
• 제21권1호
• /
• pp.89-92
• /
• 2011

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

• Journal of Advanced Marine Engineering and Technology
• /
• 제27권5호
• /
• pp.633-640
• /
• 2003

The present experimental study is focused on the application of multi-point simultaneous measurement by PIV(Particle Image Velocimetry) to guide vane region within diffuser pump. Various different kinds of rotational velocity were selected as experimental condition. Optimized cross correlation Identification to obtain velocity vectors is implemented with direct calculation of correlation coefficients. Fine optical setup concerned with PIV performance is arranged for the accurate PIV measurement of high-speed complex flow. Variable flow pattern are represented quantitatively at the stator region.

• 유체기계공업학회:학술대회논문집
• /
• 유체기계공업학회 1998년도 유체기계 연구개발 발표회 논문집
• /
• pp.11-18
• /
• 1998

The present experimental study is focused on the application of multi-point simultaneous measurement by PIV(Particle Image Velocimetry) to rotor-stator region within centrifugal turbine pump. Six different kinds of rpm(120, 500, 1000, 1500, 2000 and 2500) are selected as experimental condition. Optimized cross correlation identification to obtain velocity vectors is implemented by direct calculation of correlation coefficients. Fine optical setup deeply concerned with PIV performance is arranged for accurate PIV measurement of high-speed complex flow. The instantaneous and time-mean velocity distribution and velocity profile are represented quantitatively at the rotor and stator region.

• Structural Engineering and Mechanics
• /
• 제49권3호
• /
• pp.411-425
• /
• 2014

This paper presents the processing of nonlinear features associated with a damage event by quadratic time-frequency distributions for damage identification in a frame structure. A time-frequency distribution is a function which distributes the total energy of a signal at a particular time and frequency point. As the occurrence of damage often gives rise to non-stationary, nonlinear structural behavior, simultaneous representation of the dynamic response in the time-frequency plane offers valuable insight for damage detection. The applicability of the bilinear time-frequency distributions of the Cohen class is examined for the damage assessment of a frame structure from the simulated acceleration data. It is shown that the changes in instantaneous energy of the dynamic response could be a good damage indicator. Presence and location of damage can be identified using Choi-Williams distribution when damping is ignored. However, in the presence of damping the Page distribution is more effective and offers better readability for structural damage detection.

• 제어로봇시스템학회논문지
• /
• 제15권4호
• /
• pp.353-364
• /
• 2009

In this paper, a SLAM (Simultaneous Localization and Mapping) method using a single camera and planar fiducial markers is proposed. Fiducial markers are planar patterns that are mounted on the ceiling or wall. Each fiducial marker has a unique hi-tonal identification pattern with square outlines. It can be printed on paper to reduce cost or it can be painted using retro-reflective paint in order to make invisible and prevent undesirable visual effects. Existing localization methods using artificial landmarks have the disadvantage that landmark locations must be known a priori. In contrast, the proposed method can build a map and estimate robot location even if landmark locations are not known a priori. Hence, it reduces installation time and setup cost. The proposed method works good even when only one fiducial marker is seen at a scene. We perform computer simulation to evaluate proposed method.