[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1007.07051

Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis

Kumar, Sanjay (Division of Biotechnology, Defence Research and Development Establishment)
Tuteja, Urmil (Division of Microbiology, Defence Research and Development Establishment)
Sarika, Kumari (Division of Microbiology, Defence Research and Development Establishment)
Singh, Dhirendra Kumar (Division of Veterinary Public Health, Indian Veterinary Research Institute)
Kumar, Ashok (Division of Veterinary Public Health, Indian Veterinary Research Institute)
Kumar, Om (Division of Biotechnology, Defence Research and Development Establishment)

Publication Information

Journal of Microbiology and Biotechnology / v.21, no.1, 2011 , pp. 89-92 More about this Journal

Abstract

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

Keywords

Multiplex PCR; internal amplification control; brucellosis;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 1 (Related Records In Web of Science)

Reference
Cited By KSCI

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