• Journal of the Korean Chemical Society
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• v.37 no.5
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• pp.513-522
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• 1993

The purpose of this study is to optimize the selectivity of mobile phase solvents for separation of 25 phenols in reversed phase liquid chromatography and to accomplish the simultaneous preconcentration and separation of trace phenols from water samples. Phenols used in this study were classified into three groups, chloro-, methyl-, and nitrophenols. Quaternary solvent mobile phases were employed to improve the selectivity. Overlapping resolution maps(ORM) as a statistical simplex techniques was used to predict the optimum solvent system. Additional criterion such as pH and temperature were also investigated. In order to improve the resolution and decrease the analysis time, isoselective multisolvent gradient elution system was employed with ORM-Prism method. The simultaneous preconcentration and separation of trace phenols from water samples were performed by using XAD-2/Dowex 1-X8 tandem column. When the extraction efficiency was evaluated by sampling up to 1 L of distilled water, recovery of the phenols, except phenol, was above 90% and the limit of detection of the phenols was 5 ppb. The XAD-2/Dowex 1-X8 method was superior to C18 cartridge in terms of recovery and selectivity.

• Journal of Life Science
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• v.18 no.3
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• pp.374-380
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• 2008

Rapid detection of enterovirus (EVs) is important in the management of aseptic meningitis. We examined the relative efficiency and specificity of the real-time nucleic acid sequence-based amplification (NASBA) comparing with the established reverse transcription polymerase chain reaction (RT-PCR) and viral culture method which were used for the detection of enterovirus RNA in clinical specimens. Of the total 292 samples, 145 were found to be positive to enterovirus RNA by real-time NASBA, 101 were positive by viral culture, and 86 were positive by RT-PCR. 147 samples and 46 samples were determined to be negative and positive by all methods respectively, but 4 samples were positive only by real-time NASBA. To compare the specificity of each method, various clinical samples which were diagnosed for herpes simplex virus (HSV)-1, HSV-2, adenovirus, mumps, and rhinovirus were applied. Except one rhinovirus sample which was false positive to enterovirus RNA by RT-PCR, the other different samples were negative to all three methods. The real-time NASBA procedure can be completed within 5 hours in contrast with 9 hours for the RT-PCR and 3-14 days for the viral culture. From this study, it was suggested that the real-time NASBA assay could be a standardized, rapid, specific, and sensitive procedure for the detection of enterovirus RNA.

• Korean Journal of Head & Neck Oncology
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• v.19 no.1
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• pp.25-33
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• 2003

Background and Objectives: The Herpes Simplex type 2 Defective Infectious Single Cycle virus (DISC virus) is attenuated virus originally produced as viral vaccines but are also efficient gene transfer vehicle. The main goals of this study were to examine the efficiencies of the gene transfer using DISC vectors for various head and neck squamous cell carcinoma cell lines and to evaluate the efficacy of vaccination with DISC virus carrying a immunomodulatory genes (GM-CSF) as cancer therapy in a organotopic oral cavity squamous cell cancer model. Materials and Methods : We determinated the gene transfer efficiency of DISC virus by x-gal stain method and proved gene and protein expression of DISC-GMCSF transfected SCCVII cells by RT-PCR and ELISA method. Also we evaluated the ex vivo vaccination effects of SCCVII/GMCSF (DISC-GMCSF transfected SCCVII vaccine) vaccine on preventing the recurrence of micro-residual tumor. After the vaccination of SCCVII/GMCSF, specific cytotoxic T-cell responses was evaluated by CTL assay. Results: At an MOI of 10 DISC virus showed 64-88% of transfection rates in various head and neck squamous cancer cell lines. SCCVII cells transduced by DISC virus vector (MOI=10) carrying the GM-CSF gene, produced 4.5 nanogram quantities of GM-CSF per $10^6$ cells. In vivo vaccination using tumor cells transduced ex vivo with DISC-GMCSF resulted in better protection rate against subsequent tumor recurrence in organotopic oral cavity cancer model. Although tumor free survival rate was not statistically significantly increased in vaccination group (p=0.078), tumor specific cytotocic T-cell responses were significantly increased in SCCVII/GMCSF vaccination group. Conclusion: These data demonstrate that; 1) The DISC virus vector is capable of efficient gene transfer to various head and neck squamous cancer cell lines, 2) GM-CSF secreting genetically modified tumor vaccine (SCCVII/GMCSF) efficiently protected against tumor recurrence in organotopic micro-residual oral cavity cancer model and produced tumor specific cytotoxic T-cell response. DISC virus-mediated, cytokine gene transfer may prove to be useful as a clinical therapy for head and neck cancers.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.271-278
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• 2002

Methanol extracts from 420 samples of 173 plant species in 58 families were tested at 5000 ppm for their insecticidal and acaricidal activities against five economically important arthropod pests by spray method. The responses varied with arthropod pest species, plant species and plant tissue sampled. In a test with Nilaparvata lugens Stal, extracts from Zanthoxylum piperitum barks, Chamaecyparis obtusa leaf and Quercus salicina leaf showed potent insecticidal activity. With Plutella xylostella L., potent larvicidal activity was observed from extracts of Platycarya strobilacea wood, Meliosma myriantha barks, Sophora japonica leaf, Zanthoxylum piperitum barks, and Pinus thunbergii wood. Methanol extracts of Sophora japonica leaf and Zanthoxylum piperitum barks showed high insecticidal activity against Spodoptera litura. In a test with Tetranychus urticae Koch, extract from Carpinus coreana leaf, Firmiana simplex barks, Elaeagnus macrophylla leaf, Aralia elata leaf, Comus controversa barks and Chamaecyparis obtusa leaf exhibited strong acaricidal activity. As a naturally occurring pest control agent, Zanthoxylum piperitum barks could be useful as new insecticidal and acaricidal products against various arthropod pests.

• Journal of the Korean Chemical Society
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• v.33 no.3
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• pp.287-294
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• 1989

The optimum condition for the separation of priority pollutant phenols using isocratic elution has been determined. The elution behavior of eleven phenols has been also studied to interpret the retention. The reversed phase liquid chromatographic methods were performed on a ${\mu}$-Bondapak $C_{18}$ column with methanol-water, acetonitrile-water, and THF water mixtures as mobile phases. The COF method, where Snyder's solvent triangle concept was combined with a mixture-design statistical technique, was used to optimize the strength and selectivity of solvents for the separation of phenols. The optimum solvent composition, which gives a complete separation of eleven phenols, was found to be $MeOH:ACN:H_2O$ = 7:40:53. The plots of ln k' vs. -${\Delta}H^{\circ}$ and ${\Sigma}{\pi}$ of phenols showed relatively good linearities. Effect of van der Waals volume, pi-energy and hydrogen bonding on the retention of phenols were investigated. The following equation with the correlation coefficient of 0.9927 for ACN-water solvent system was obtained; $log^{k'}=2.515{\times}10^{-2}VWV-1.301{\times}10^{-1}E-3.674{\times}10^{-1}$

• Journal of Environmental Science International
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• v.32 no.1
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• pp.47-55
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• 2023

This study evaluated the biochemical methane potential (BMP) of primary sludge, secondary sludge, and food waste in batch anaerobic mono-digestion tests, and investigated the effects of mixture ratio of those organic wastes on methane yield and production rate in batch anaerobic co-digestion tests, that were designed based on a simplex mixture design method. The BMP of primary sludge, secondary sludge and food waste were determined as 234.2, 172.7, and 379.1 mL CH₄/g COD, respectively. The relationships between the mixing ratio of those organic wastes with methane yield and methane production rate were successfully expressed in special cubic models. Both methane yield and methane production rate were estimated as higher when the mixture ratio of food waste was higher. At a mixing ratio of 0.5 and 0.5 for primary sludge and food waste, the methane yield of 297.9 mL CH₄/g COD was expected; this was 19.4% higher than that obtained at a mixing ratio of 0.3333, 0.3333 and 0.3333 for primary sludge, secondary sludge, and food waste (249.5 mL CH₄/g COD). These findings could be useful when designing field-scale anaerobic digersters for mono- and co-digestion of sewage sludges and food waste.

• Korean Journal of Environmental Agriculture
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• v.13 no.3
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• pp.251-261
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• 1994

This study was conducted to get some basic information about rice plant development rate and heading ecology in various climatic conditions, growing nine varieties at three locations, Jinbu as the mountainous cool area, Suweon and Iri as the plain area for two years from 1987 to 1988. Average daily air temperature and day length from transplanting to heading date were analyzed in relation to the heading. Heading date and development rate of each variety were estimated by the Symplex method and the fitness of the model was evaluated. The results obtained as follows: Average daily air temperatures among varieties during the period from transplanting to heading ranged from 18 to $19^{\circ}C$ in Jinbu, from 22.5 to $23.5^{\circ}C$ in Suweon, and from 23.5 to $24.5^{\circ}C$ in Iri, the late-maturing varieties requiring the higher temperatures. The average heading days were about 20 days longer in Jinbu and $3{\sim}5$ days shorter in Iri than those in Suweon in all varieties. Little differences in accumulated temperature from transplanting to heading were observed in regions and years, and also among varieties. Developmental stages could be expressed as the accumulation of daily development rate and the predicted heading dates by the Symplex method were similar to the observed ones. The development rate of each variety varies with air temperatures. The early maturing Japonica types including the Unbong variety were fast in development rate at lower temperatures, but the late-maturing varieties of the Japonica type were late. A model to predict the heading dates of rice varieties by the Symplex method using air temperatures and day lengths was feasible.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.729-738
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• 1997

To estimate the change in rice productivity around lake Juam due to construction of artificial lake, growth, yield components and yield of rice were measured at different locations around lake Juam for three years from 1994 to 1996. Automated weather stations(AWS) were installed nearby the experimental paddy fields, and daily maximum, average and minimum temperature, solar radiation, relative humidity, and precipitation were measured for the whole growing period of rice. Plant height, number of tillers, leaf area and shoot dry weight per hill were observed from 8 to 10 times in the interval of 7 days after transplanting. Yield and yield components of rice were observed at the harvest time. Simulation model of rice productivity used in the study was SIMRIW developed by Horie. The observed data of rice at 5 locations in 1994, 3 locations in 1995 and 4 locations in 1996 were inputted in the model to estimate the unknown parameters. Comparisons between observed and predicted values of shoot dry weights, leaf area indices, and rough rice yield were fairly well, so that SIMRIW appeared to predict relatively well the variations in productivity due to variations of climatic factors in the habitat. Climatic elements prior to as well as posterior to dam construction were generated at six locatons around lake Juam for thirty years by the method of Pickering et al. Climatic elements simulated in the study were daily maximum and minimum temperature, and amount of daily solar radiation. The change in rice productivity around lake Juam due to dam construction were estimated by inputting the generated climatic elements into SIMRIW. Average daily maximum temperature after dam construction appeared to be more or less lower than that before dam construction, while average daily minimum temperature became higher after dam construction. Average amount of daily solar radiation became lower with 0.9 MJ $d^{-1}$ after dam construction. As a result of simulation, the average productivity of habitats around lake Juam decreased about 5.6% by the construction of dam.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.15-20
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• 2008

Purpose : Large exon deletions in the DMD gene are found in about 60% of DMD/BMD patients. Multiplex PCR has been employed to detect the deletion mutation, which frequently generates noise PCR products due to the presence of multiple primers in a single reaction as well as the stringency of PCR conditions. This often leads to a false-negative or false-positive result. To address this problematic issue, we introduced the dual primer oligonucleotide (DPO) system. DPO contains two separate priming regions joined by a polydeoxyinosine linker that results in high PCR specificity even under suboptimal PCR conditions. Methods : We tested 50 healthy male controls, 50 patients with deletion mutation as deletion-positive patient controls, and 20 patients with no deletions as deletion-negative patient controls using DPO-multiplex PCR. Both the presence and extent of deletion were verified by simplex PCR spanning the promoter region (PM) and 18 exons including exons 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, and 60 in all 120 controls. Results : DPO-multiplex PCR showed 100% sensitivity and specificity for the detection a deletion. However, it showed 97.1% sensitivity and 100% specificity for determining the extent of deletions. Conclusion : The DPO-multiplex PCR method is a useful molecular test to detect large deletions of DMD for the diagnosis of patients with DMD/BMD because it is easy to perform, fast, and cost-effective and has excellent sensitivity and specificity.

• Journal of the Korea Society of Computer and Information
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• v.24 no.12
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• pp.51-57
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• 2019

In this paper, we analyzed the performance of IoT based smart wearable mine detection device. There are various mine detection methods currently used by the military. Still, in the general field, mine detection is performed by visual detection, probe detection, detector detection, and other detection methods. The detection method by the detector is using a GPR sensor on the detector, which is possible to detect metals, but it is difficult to identify non-metals. It is hard to distinguish whether the area where the detection was performed or not. Also, there is a problem that a lot of human resources and time are wasted, and if the user does not move the sensor at a constant speed or moves too fast, it is difficult to detect landmines accurately. Therefore, we studied the smart wearable mine detection device composed of human body antenna, main microprocessor, smart glasses, body-mounted LCD monitor, wireless data transmission, belt type power supply, black box camera, which is to improve the problem of the error of mine detection using unidirectional ultrasonic sensing signal. Based on the results of this study, we will conduct an experiment to confirm the possibility of detecting underground mines based on the Internet of Things (IoT). This paper consists of an introduction, experimental environment composition, simulation analysis, and conclusion. Introduction introduces the research contents such as mines, mine detectors, and research progress. It consists of large anti-personnel mine, M16A1 fragmented anti-mine, M15 and M19 antitank mines, plastic bottles similar to mines and aluminum cans. Simulation analysis is conducted by using MATLAB to analyze the mine detection device implementation performance, generating and transmitting IoT signals, and analyzing each received signal to verify the detection performance of landmines. Then we will measure the performance through the simulation of IoT-based mine detection algorithm so that we will prove the possibility of IoT-based detection landmine.