• 한국잠사곤충학회지
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• 제53권2호
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• pp.124-129
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• 2015

본 실험에서는 누에 피브로인 L-chain 유전자의 인트론 영역을 사용하여 우리나라에서 보존하고 있는 누에 계통중 원산지별 누에 계통 8개를 대상으로 계통 간 유전자다형성을 비교하였다. 실험 결과, 실험에 사용한 누에 계통에 따라서 동일한 계통 내 모든 개체에서 FibL1과 FibL2의 유전자다형성 조합이 일정하게 유지되어 있었으며, 계통 간에는 FibL1과 FibL2의 유전자 다형성 패턴 조합이 다소 상이한 것으로 확인되었다. 따라서, 프라이머 FibL1와 FibL2에 의한 피브로인 L-chain 유전자 다형성 염기서열 정보를 활용한다면 제한된 영역에서 누에 계통 구분을 위한 지표로 활용할 수 있을 것으로 사료된다.

• 한국잠사곤충학회지
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• 제50권1호
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• pp.5-9
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• 2012

수옥잠은 부화비율, 화용비율이 높은 강건 다수성 애누에 인공사료 적합품종을 육성하였다. 이 품종의 일본종계 원종 잠157은 2003년 K305와 9671을 교잡 육성한 무늬품종이고 중국종계 잠158은 1997년에 CBJ와 9032을 교잡 육성한 선발 육성한 무늬 없는 품종이다. 실용 부화비율은 97%, 화용비율은 95.9%로 우수하였고, 전견중이 2.40 g, 1만두 수견량은 22.6 kg, 상견비율 95.5%로 대조 품종과 비슷한 성적을 나타냈다. 실켜기 성적에서 견사장은 대조 1,433 m보다 78 m 짧고, 견사량이 대조 보다 적은 49.6 cg 이었다. 견사섬도는 3.31 d이었다. 이품종의 잠종생산능력은 일본종계 원종 잠157의 산란수는 513개로 대조 대풍잠의 잠151의 525개와 비슷하였고, 중국종계 원종 잠1568 산란수 522개로 대조 잠152의 447보다 105개가 많아 산란성이 우수하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.129-133
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• 2004

The breeding work was initiated by utilizing two Japanese hybrids namely C135${\times}$N134 and N137${\times}$C146 along with two evolved Indian breeds, J14 and A24. The breed CSR16 which is characterized with marked larvae and white dumbbell cocoons was evolved from the Japanese hybrid C135${\times}$N134 by crossing with J14, while the breed CSR17 which is characterized with plain larvae and white oval cocoons was evolved from the Japanese hybrid N137${\times}$C146 by crossing with A24. The hybrid of these evolved breeds i.e., CSR16${\times}$CSR17 has shown superior over control hybrid $KA{\times}{NB_4}{D_2}$ and on par with the ruling hybrid of CSR2${\times}$CSR4. The hybrid CSR16${\times}$CSR17 was authorized during 1999 by Central Silk Board, Bangalore, Government of India for commercial exploitation during favourable months based on national level race authorization test.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.121-126
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• 2002

Breeding programme was initiated during November, 1995 with the main objective to breed productive multivoltine breeds/ hybrids with suitable genetic constitution to suit the fluctuating tropical climate prevailed in India. Two multivoltine breeds viz., BL-24 and BL-27 selected were as breeding resource materials from the silkworm germplasm maintained at Central Sericul-tural Research and Training Institute, Mysore. By adopting hybridization, backcrossing inbreeding and selection, a new multivoltine breed namely BL-67. This breed spins light greenish yellow cocoons and cocoon shape is oval with medium to coarse grains. The evolved breed was crossed with five tropical bivoltine breeds viz., NB4D2, CSR2, CSR5, CSR18 and CSR101 to study the combining ability, and identified a superior hybrid, BL67 ${\times}$ CSR101, named as CAUVERY, The hybrid is characterized by high pupation rate (>95%), high shell weight (> 35 cg), high cocoon shell ratio (> 20%), longer filament length (> 900 m) and high neatness (93) with a renditta of 6.5 producing 2A-3A grade silk. The hybrid is selected for Race Autho-rization test of Central Silk Board.

• 한국잠사곤충학회지
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• 제48권2호
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• pp.37-40
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• 2006

다수성 품종으로 육성된 대풍잠은 새로운 봄누에 품종으로서 육성경위와 주요특성을 요약하면 다음과 같다. 1. 일본종계 원종 잠151은 도입 육성한 무늬 품종이며, 중국종계 원종 잠152는 교잡 육성한 강건다수성 한성무늬 누에품종이다. 2. 2005년 춘잠기 지역적응시험 결과는 대풍잠이 다수견, 다사량의 특성을 지닌 금옥 잠에 비해 전견중이 무거워 고치수량이 6% 증수되었다. 3. DNJ 함량은 대조 금옥잠 4.26 mg과 같은 수준인 4.22 mg으로 함량이 우수한 편이었고, 동충하초 생산력 검정에서 동충하초 생체중이 1개당 1.61 g으로 대조 1.41 g 보다 14% 무거워 다수성 특성을 나타냈다. 4. 조사성적에서는, 견사장은 1,536 m로 대조대비 지수로 8%가 떨어지나, 섬도가 굵고 견사량이 많아 1만두 생사량이 5.24 kg/만두로 대조 대비 지수로 4% 높아 다사량의 특성을 나타내었다. 5. 잠종생산능력에 있어서는 일본종계 원종 잠151의 나방당 산란수는 564개로 대조와 비슷하였다. 중국종계 원종 잠152의 산란수는 588개로 일반장려품종의 원종에 비해 산란성이 우수하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.15-20
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• 2003

The present investigation was carried out to study the possible cause for reciprocal difference in silkworm hybrids. By utilising the polyvoltine race Pure Mysore (PM) and newly evolved breeds (CSR2, CSR5, CSR16 and CSR17), the direct and reciprocal crosses of polyvoltine ${\times}$ bivoltine and also bivoltine hybrids were studied. The hybrids of polyvoltine ${\times}$ bivoltine (direct) are superior to their reciprocal crosses in respect of cocoon yield, cocoon weight and filament length. The reciprocal crosses of polyvoltine ${\times}$ bivoltine are superior to their direct crosses in respect of fecundity and short larval duration. No significant differences were observed in the characters like cocoon shell ratio, raw silk percentage, denier, reelability and neatness in both polyvoltine ${\times}$ bivoltine direct crosses and their reciprocals. The expression of cocoon characters as a function of sex revealed that direct crosses (polyvoltine ${\times}$ bivoltine) showed higher cocoon weight, pupal weight, shell weight and longer filament length in females than the reciprocal crosses (bivoltine ${\times}$ polyvoltine), where as these characters in males were almost the same in both direct and reciprocal crosses, indicating that the sex-linked genetic factor played a more important role. it was clear that difference in cocoon yield observed in reciprocal crosses of polyvoltine ${\times}$ bivoltine was due to the low cocoon and shell weight in females which was turn due to presence of early maturity genes (Lme) linked with sex-chromosome (X) which effect on larvae period of the silkworm. In bivoltine hybrids, i.e., both direct and their reciprocals crosses, all the characters viz., hatching percentage, larval duration, survival, cocoon weight, cocoon shell weight, cocoon shell ratio, raw silk percentage, filament length, denier, reelability and neatness did not show any significant difference (except number of eggs laid by moth) which could account for presence of same maturity genes (Lm) in both direct and reciprocal crosses. it was clear that reciprocal differences occur when the hybrids are prepared from the parental strains with different voltinism.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.97-98
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• 2003

Current study was aimed to understand an interaction between Dopa decarboxylase (DDC) and proteins that specifically binds to DDC in the silkworm, Bombyx mori. Materials and Methods: Materials-Animal: Bombyx mori Construction of GST fusion protein Preparation of lysates: Protein extracted from whole body of Bombyx mori Methods-In vitro binding assay with lysates, Peptide sequence and RACE-PCR (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.15-19
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• 2003

The study evaluated the influence of feeding different maturity leaves i.e., tender, medium, coarse and mixed leaves of three different maturity during late larval stages of new bivoltine race $(CSR_{3} {\times} CSR_{6})$. The data were compared with shoot feeding and control batches in which conventional feeding method was followed. The most of the larval and cocoon characters were recorded significantly higher in tender leaves fed batches followed by T4 batch (2 times tender and 1 time coarse leaves). Lowest melting percentage (1.494%) was recorded in T4 and highest (4.69%) was recorded in coarse leaf (T3) fed batches. Significantly higher post cocoon parameters viz., average filament length, non-breakable filament length, renditta and raw silk percentage were recorded in tender loaves fed batches.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권2호
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• pp.191-195
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• 2003

The impact of antibiotic (Norfloxacin) administration ,at different concentration (50 ppm and 100 ppm) on commercial characters of bivoltine $({CSR_2}{\times}{CR_4})$ and the cross breed race $({BL_67}{\times}{CSR_101})$ was studied. The result indicated that antibiotic administration with different concentrations significantly improved the rearing and cocoon parameters like larval duration, larval weight, growth index, single cocoon weight, single shell weight and shell ratio. The post cocoon parameters like average filament length, non-breakable filament length, raw silk percentage, raw silk recovery percentage, denier, reelability and neatness were recorded significantly higher in antibiotic treated batches. The better performances of these parameters were recorded with the increase of antibiotic concentration.

• 한국잠사곤충학회지
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• 제50권1호
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• pp.1-4
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• 2012

대박잠은 기존 보급 누에품종보다 화용비율이 높은 강건성 품종으로 대조 품종대비 5령 3일 유충 무게가 10% 무겁고, 전견중이 무거워 수량성이 높은 다수성 품종으로 육성하게 되었다. 이 품종의 일본종계 원종 잠155는 1999년 NB7과 NB18을 교잡 육성한 무늬 없는 품종이고 중국종계 잠156은 1999년에 도입한 CB을 선발 육성한 무늬없는 품종이다. 이 품종의 사육성적은 화용비율이 96.4%, 전견중이 2.72 g, 1만두 수견량은 25.4 kg으로 대조 품종보다 우수하였다. 실켜기 성적에서 견사장이 1,506 m, 견사량이 53.2 cg으로 1만두 생사량 4.92 kg으로 많아 우수하였고, 견사섬도는 3.19 d 이었다. 잠종생산능력은 일본종계 원종 잠155의 1나방당 산란수는 533개, 중국종계 원종 잠156의 산란수 629개로 산란성이 우수하였다.