한국잠사곤충학회지 (Journal of Sericultural and Entomological Science)

한국잠사학회 (Korean Society of Sericultural Science)
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누에 피브로인 유전자 다형성 마커 개발

Development of polymorphism genetic marker for identification of the silkworm races

  • 최광호 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김성렬 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 강석우 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 박옥란 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김성완 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김기영 (농촌진흥청 국립농업과학원 잠사양봉소재과)
  • Choi, Kwang-Ho (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Piao, Yulan (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Sung-Wan (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Kee-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • 투고 : 2015.10.13
  • 심사 : 2015.10.26
  • 발행 : 2015.10.31
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초록

본 실험에서는 누에 피브로인 L-chain 유전자의 인트론 영역을 사용하여 우리나라에서 보존하고 있는 누에 계통중 원산지별 누에 계통 8개를 대상으로 계통 간 유전자다형성을 비교하였다. 실험 결과, 실험에 사용한 누에 계통에 따라서 동일한 계통 내 모든 개체에서 FibL1과 FibL2의 유전자다형성 조합이 일정하게 유지되어 있었으며, 계통 간에는 FibL1과 FibL2의 유전자 다형성 패턴 조합이 다소 상이한 것으로 확인되었다. 따라서, 프라이머 FibL1와 FibL2에 의한 피브로인 L-chain 유전자 다형성 염기서열 정보를 활용한다면 제한된 영역에서 누에 계통 구분을 위한 지표로 활용할 수 있을 것으로 사료된다.

We have previously characterized the complete fibroin light chain gene from one of the silkworm race Baegokjam (Bombyx mori) and found two variable regions (FibL1, intron 2 ~ 3; FibL2, intron 6) with the primer sets designed to cover these variable regions. In this study, we tested the utility of these variable regions as genetic markers for classifying silkworm races. For the purpose, Europian races (Q, PK), Chinese races (C26, C31), Japanese races (N15, N9), and tropical races (SA2, SA5) were used in this experiment. The polymorphism of the FibL1 and FibL2 were divided into two and three types, respectively. The combination of the FibL1 and FibL2 polymorphisms were constant within the same races. The result suggest that the primer sets designed from two variable regions of fibroin light chain gene may be useful as the genetic markers for silkworm races.

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참고문헌

  1. Choi KH, Kang SW, Kang PD, Goo TW, Hwang JS, Yun EY, Lee SM, Sohn HD, Jin BR (2003) The use of fibroin light chain gene sequence for the genetic marker of the silkworm race. Int J Ind Entomol 6(1), 45-48.
  2. Hwang JS, Lee JS, Kang HA, Lee SM, Suh DS (1995) Analysis of genetic relationships among the silkworm, Bombyx mori, strains using RAPD-PCR. Korean J Genetics 17, 291-300.
  3. Inoue S, Tanaka K, Arisaka F, Kimura S, Ohtomo K, Mizuno S (2000) Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, Lchain, and P25, with a 6:6:1 molar ratio. J Biol Chem 275(51), 40517-40528. https://doi.org/10.1074/jbc.M006897200
  4. Kirby DA, Muse SV, Stephan W (1995) Maintenance of PremRNA secondary structure by epistatic selection. Proc Natl Acad Sci 92, 9047-9051. https://doi.org/10.1073/pnas.92.20.9047
  5. Matteo F, Filipe GV, Thorfinn SK, Tyler L, Emilia HS, Anders A, Rasmus N (2013) Quantifying population genetic differentiation from next-generation sequencing data. Genetics 195(3), 979-992. https://doi.org/10.1534/genetics.113.154740
  6. Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki H, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin-I T, Abe H, Shimada T, Morishita S, Sasaki T (2004) The genome sequence of silkworm, Bombyx mori. DNA Res 29, 27-35.
  7. Mita K, Morimyo M, Okano K, Koike Y, Nohata J, Kawasaki H, Kadono-Okuda, K, Yamamoto K, Suzuki MG, Shimada T, Goldsmith MR, Maeda S (2003) The construction of an EST database for Bombyx mori and its application. Proc Natl Acad Sci 100, 14121-14126. https://doi.org/10.1073/pnas.2234984100
  8. Nho SG, Lee JM (2000) Characteristics of Korean native strains in the domesticated silkworm, Bombyx mori. Korean J Seric Sci 42(1), 10-13.
  9. Ote M, Mita K, Kawasaki H, Seki M, Nohata J, Kobayashi M, Shimada T (2004) Microarray analysis of gene expression profiles in wing discs of Bombyx mori during pupal ecdysis. Insect Biochem Mol Biol 34, 775-784. https://doi.org/10.1016/j.ibmb.2004.04.002
  10. Schaeffer SW, Miller EL (1993) Estimates of linkage disequilibrium and the recombination parameter determined from segregating nucleotide sites in the alcohol dehydrogenase region of Drosophila pseudoobscura. Genetics 135, 541-552.
  11. Seong SI (1997) Genetic relationships of silkworm stocks in Korea inferred from isozyme analysis. Korean J Seric Sci 39, 119-133.