• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.954-961
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• 2017

We investigated the purifications of PE-gal and CPN-gal, synthesized by transgalactosylation reaction using recombinant ${\beta}$-gal. The reaction mixture containing PE and PE-gal was first mixed with EA, and thereafter PE and PE-gal were distributed in two-phase (EA/water) system. In this system, PE and PE-gal was selectively moved into EA and water phase, respectively. Then, the water phase was collected, and silica gel chromatography was carried out using the collected water phase. Finally, we compared two purified PE-gal samples using HPLC and TLC analysis, in which the one was purified only by silica gel chromatography and the other was purified by EA extraction followed by silica gel chromatography. In the latter case, the residual PE was almost completely removed, whereas, in the former case, the residual PE was remained remarkably. Additionally, the purification yield of PE-gal was about 21% on the basis of mole. In the same purification protocol, CPN-gal was able to be purified using EA extraction followed by silica gel chromatography, in which the residual CPN was almost removed when CPN-gal was purified by EA extraction followed by silica gel chromatography.

• Korean Journal of Materials Research
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• v.25 no.4
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• pp.209-213
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• 2015

Synthesis of nano-silica using water glass in a Sol-Gel process is one of several methods to manufacture nano-silica. In nano-silica synthesized from water glass, there are various metal impurities. However, synthesis of nano-silica using water glass in a Sol-Gel process is an interesting method because it is relatively simple and cheap. In this study, nano-silica was synthesized from water glass; we investigated the effect of pH on the synthesis of nano-silica. The morphology of the nanosilica with pH 2 was flat, but the surface of the nano-silica with pH 10 had holes similar to small craters. As a result of ICP-OES analysis, the amount of Na in the nano-silica with pH 2 was found to be 170 mg/kg. On the other hand, the amount of Na in the nano-silica with pH 10 was found to be 56,930 mg/kg. After calcination, the crystal structure of the nano-silica with pH 2 was amorphous. The crystal structure of the nano-silica with pH 10 transformed from amorphous to tridymite. This is because elemental Na in the nano-silica had the effect of decreasing the phase transformation temperature.

• Tribology and Lubricants
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• v.13 no.3
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• pp.115-123
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• 1997

The electrorheological (ER) behavior of suspensions in silicone oil of silica gel powder (average particle size 49 $\mu$m) absorbed water was investigated at room temperature with electric fields up to 2.4 KV/mm. In this paper, for development of succcessful ER fluids used for wide temperature range later, we would like to know a fundamental understanding of water on ER effect. As a first step, the ER fluids involving water activated silica gel were measured not only the electrical characteristics such as dielectric constant, current density and electrical conductivity but also the rheological properties on the strength of electric field, the quantity of dispersed phase and absorbed water. From the experimental results that water absorbed to the particles directly affects to the surface charge density of electric double layer model proposed by Schwarz and makes dielectric constant and current density of ER fluids increase. The current density and dynamic yield stress $($\tau$_y)$ of water activated silica gel suspensions was in exponential proportion to the strength of electric field, the quantity of dispersed phase and absorbed water. And the optimum water quantity and weight concentration of silica gel for electrorheological effect were 4-5 wt% and 15 wt%, respectively.

• Journal of the Korean Ceramic Society
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• v.24 no.5
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• pp.437-446
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• 1987

In this study the attempts were made to investigate the possibilities of using domestic clay minerals and zeolite, which are available in almost unlimitted quantities, for preparation of aluminum sulfate, white carbon and silica gel. Aluminum sulfate was obtained by the method of sulfuric acid treatment. White carbon and silica gel were prepared by utilizing crystalline silica which was obtained as a by product during preparing the aluminum sulfate. White carbon was obtained by reacting 5.0 weight percent sulfuric acid solution with water glass which was a reaction product of crystalline silica and soldium hydroxide in a autoclave, while silica gel was obtained by reaction 10.0 weight percent acetic acid solution with water glass.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.5
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• pp.2529-2535
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• 2013

Sol-gel method was utilized to prepare SPEEK/PWA electrolyte composite membranes. TEOS was used as a precursor and phosphotungstic acid(PWA) as a catalyst for the sol-gel reaction. It was observed through FE-SEM analysis that the PWA and silica nanoparticles were uniformly dispersed into the polymer matrix. The water uptake of SPEEK/PWA/silica composite membranes was less affected by TEOS concentration at higher TEOS contents, while the water uptake decreased as TEOS concentration increased at lower TEOS contents. The proton conductivity of the composite membranes showed similar trend as the water uptake of the composite membranes. The methanol permeability of SPEEK/PWA/silica composite membranes decreased as TEOS concentration increased.

• Journal of Power System Engineering
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• v.15 no.4
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• pp.75-78
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• 2011

There are various types of materials used in electronic industry, such as electrode material, conductor, insulator, anode, cathode and semiconductor. Electrode material type is Cu, Ti, ZnO and so on. Especially if we use mixed ZnO in soil cement or silica gel, we can have advantages in ice road to prevent freezing. We have great impact if we use supported in inorganic substances like silica gel. In this paper we have studied that ZnO supported silica gel and its properties. Zinc acetate dissolved in distilled water were loaded on the silica gel by the reaction with ammonia at $80^{\circ}C$. And we investigated particle structures of ZnO by scanning electron microscopy(SEM) and X-ray diffraction(XRD).

• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1516-1520
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• 2009

A sensitive technique for the determination of trace Co(II) in various samples after column preconcentration by adsorbing onto silica gel loaded with 1-nitroso-2-naphthol was developed. Several experimental conditions, such as pH of sample solution, the amount of silica gel loaded with 1-nitroso-2-naphthol, the flow rate for adsorption and so forth, were optimized. The interfering effects of diverse concomitant ions were investigated. Fe(III) interfered with more than any other ions, but the interference by Fe(III) was completely eliminated by adjusting the amount of silica gel loaded with 1-nitroso-2-naphthol to 0.30 g. The dynamic range, the correlation coefficient ($R^2$), and the detection limit obtained by the proposed technique were 3.0-140.0 ng m$L^{-1}$, 0.9942, and 1.81 ng m$L^{-1}$, respectively. For validating the technique, the aqueous samples (tap water, reservoir water, stream water, and wastewater) and the plastic samples were used as real samples. Recovery yields of 93.0-107.0% were obtained. These measured data were not different from ICP-MS data at the 95% confidence level by F test. Based on the results of the experiment, it has been found that the proposed technique can be applied to the determination of Co(II) in various real samples.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.2
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• pp.150-155
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• 2001

It has been observed that water, which is absolutely essential for enzyme activity, can induce the agglomeration of enzyme particles in organic media. Although enzyme agglomeration is significant in that it usually reduces enzyme activity and stability, little attention has been paid to the quantitative analysis of enzyme agglomeration behavior in nonaqueous biocatalytic systems. In this study, the effect of water and silica gel on enzyme agglomeration were investigated using Candida rugosa lipase and cyclohexane as a model enzyme and an organic medium. The extent of enzyme agglomeration was quantified by sieve analysis of freeze-dried agglomerates. Increasing the water content of the medium increased the size of the enzyme agglomerates, and it was found that water produced during the esterification reaction could also promote the agglomeration of enzyme particles suspended in organic media. On the other hand, the size of the enzyme agglomerates was remarkably reduced in the presence of silica gel at the same water content. We also show that this increase in the size of enzyme agglomerates results in lower reaction rates in organic solvents.

• Korean Journal of Materials Research
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• v.24 no.5
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• pp.271-276
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• 2014

Silica nano-powder (SNP) is an inorganic material able to provide high-performance in various fields because of its multiple functions. Methods used to synthesize high purity SNP, include crushing silica minerals, vapor reaction of silica chloride, and a sol-gel process using TEOS and sodium silicate solution. The sol-gel process is the cheapest method for synthesis of SNP, and was used in this study. First, we investigated the shape and the size of the silica-powder particles in relation to the variation of HCl and sodium silicate concentrations. After drying, the shape of nano-silica powder differed in relation to variations in the HCl concentration. As the pH of the solution increased, so did the density of crosslinking. Initially, there was NaCl in the SNP. To increase its purity, we adopted a washing process that included centrifugation and filtration. After washing, the last of the NaCl was removed using DI water, leaving only amorphous silica powder. The purity of nano-silica powder synthesized using sodium silicate was over 99.6%.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.2 s.257
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• pp.116-124
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• 2007

An adsorption chiller is expected to have high energy-efficiency in utilizing the waste heat exhausted from a process. The objective of this paper is to investigate the performance of silica gel-water adsorption chiller from the cycle simulation and to provide a guideline for design of the adsorption chiller. The effect of cycle time, inlet temperature and water flow rate on the cooling capacity and COP is quantified during the cycle operation. It is found that the performance of adsorption chiller is more sensitive to the change of inlet water temperature rather than the water flow rate. It is concluded that the COP is 0.57 in the standard conditions(hot water $80^{\circ}C$, cooling water $30^{\circ}C$, chilled water inlet temperatures $14^{\circ}C$ and cycle time 420sec).