Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Water and Silica Gel on Enzyme Agglomeration in Organic Solvents

  • Keehoon Won (Department of Chemical Engineering, Division of Molecular and Life Sciences, and Institute of Environmental and Energy Technology, Pohang University) ;
  • Lee, Sun-Bik (Department of Chemical Engineering, Division of Molecular and Life Sciences, and Institute of Environmental and Energy Technology, Pohang University)
  • Published : 2001.03.01
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Abstract

It has been observed that water, which is absolutely essential for enzyme activity, can induce the agglomeration of enzyme particles in organic media. Although enzyme agglomeration is significant in that it usually reduces enzyme activity and stability, little attention has been paid to the quantitative analysis of enzyme agglomeration behavior in nonaqueous biocatalytic systems. In this study, the effect of water and silica gel on enzyme agglomeration were investigated using Candida rugosa lipase and cyclohexane as a model enzyme and an organic medium. The extent of enzyme agglomeration was quantified by sieve analysis of freeze-dried agglomerates. Increasing the water content of the medium increased the size of the enzyme agglomerates, and it was found that water produced during the esterification reaction could also promote the agglomeration of enzyme particles suspended in organic media. On the other hand, the size of the enzyme agglomerates was remarkably reduced in the presence of silica gel at the same water content. We also show that this increase in the size of enzyme agglomerates results in lower reaction rates in organic solvents.

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References

  1. Angew. Chem. Intl. Ed. v.39 properties and synthetic applications of enzymes in organic solvents Carrea G.;S. Riva
  2. Nature v.409 Improving enzymes by using them in organic solvents Klibanov A.M.
  3. CHEMTECH v.16 Enzymes that work in organic solvents Klibanov A.M.
  4. J. Mol. Cata. B Enzymatic v.2 Enhanced catalytic efficiency of enzymes in organic media with the addition of the solid support: effect of silica gel on reaction rates and enzyme agglomeration Kim M.G.;S.B. Lee
  5. Nature v.170 Micro-spherical aggregation of barium sulphate Stock D.I.
  6. Ind. Eng. Chem. Process Design Develop. v.6 Formation of spheres from finely divided solids in liquid suspension Capes C.E.;J.P. Sutherland
  7. Powder Technol. v.13 Further studies of the kinetics of spherical agglomeration in a stirred vessel Kawashima Y.;C.E. Capes
  8. Powder Technol. v.30 The physicochemical parameters determining the size of agglomerate prepared by the wet spherical agglomeration technique Kawashima Y.;K. Furukawa;H. Takenaka
  9. Int. J. Pharm. v.101 Wet spherical agglomeration of proteins as a new method to prepare parenteral fast soluble dosage forms Bausch A.;H. Leuenberger
  10. Deru Dev. Ind. Pharm. v.22 A study of the emechanisms of wet spherical agglomeration of pharmaceutical powders Chow A.H.L.;M.W.M. Leung
  11. Appl. Microbiol. Biotechnol. v.35 Enzymatic esterification in organic media: role of water and organic solvent in kinetics and yield of butyl butyrate synthesis Monot F.;F. Borzeix;M. Bardin;J.-P. Vandecasteele
  12. Biotechnol. Bioeng. v.47 A comparison of lipase-catalyzed ester hydrolysis in reverse micelles, organic solvents, and biphasic systems Yang F.;A.J. Russell
  13. Biotechnol. Bioeng. v.52 Extraordinary enantiospecificity of lipase catalysis in organic media induced by purification and catalyst engineering Tsai S.-W.;J.S. Dordick
  14. Biotechnol. Bioeng. v.44 Lipase catalyzed esterification of glycidol in nonaqueous solvents: solvent effects on enzymatic activity Martins J.F.;T.C. Sampaio;I.B. Carvalho;S. Barreiros
  15. Biochim. Biophys. Acta v.957 Enzymatic ester synthesis with continuous measurement of water activity Goldberg M.;F. Parvaresh;D. Thomas;M.-D. Legoy
  16. Biotechnol. Lett. v.15 Lipase catalysed esterification of isosorbide and sorbitol Mukesh D.;D. Sheth;A. Mokashi;J. Wagh;J.M. Tilak;A.A. Banerji;K.R. Thakkar
  17. J. Ferment. Bioeng. v.81 Enzymatic resolution of racemic ibuprofen by lipase-catalyzed esterification reaction: effects of water content and solid supports Kim M.G.;S.B. Lee
  18. Biocatal. Biotransform v.12 Internal mass transfer limitation during enzymatic esterification in supercritical carbon dioxide and hexane Bernard P.;D. Barth
  19. Enzyme Microb. Technol. v.14 Role of diffusion in nonaqueous enzymology Kamat S.;E.J. Beckman;A.J. Russell