• 한국초지조사료학회지
• /
• 제15권3호
• /
• pp.198-206
• /
• 1995

본 연구는 1991년부터 1993년까지 사료작물과 목초류 7종(호맥, 대맥, 연맥, Italian ryegrass, alfalfa, orchardgrass 및 혼과목초)을 공시재료로 하여 곤포사일리지(BS=baled silage making)조제이용을 위한 수확시기와 이때의 BS 생산성 및 품질을 평가하였다. 시험방법에 있어서 작물재배는 농촌진흥청의 사료작물 및 목초 표준경종방법에 준하여 실시하였다. 사일리지 조제작업중 BS는 자체적으로 제작한 각형 베일러를 이용 개체당 크기 90cm$\times$60cm$\times$50cm, 중량은 건물기준 20kg 내외, 곤포압착 21~23mm를 유지하여 조제하였다. 한편 고수분 재료는 수분정도에 따라 예건처리(무처리, 0.5일 및 1.0일)와 formic acid(무처리, 0.3% 및 0.6%)를 병행 처리하여 BS 를 조제하였다. 맨류작물의 BS 생산을 위한 수확적기는 대맥, 황숙기, 호맥과 연맥은 유숙기 이었으며 이때의 BS 수량은 각각 11.92톤(대맥), 12.64톤(호맥) 및 8.41톤/ha(연맥)이었다. Italian ryegrass 및 목초의 수확적기는 각 초종 모두 개화기로서 년간 BS 생산성은 Italian 13.81톤(2회 예취), 혼파목초 11.46톤(3회), alfalfa 10.62톤/ha(3회)이었다. 한편 조기수확에 따른 고수분 재료의 BS 품질은 무처리시의 35점에서 예건 및 FA 동시처리시에는 84점으로 향상되었다. Italian tyegrass의 BS품질도 무처리시의 61점에서 예건 및 FA 처리시에는 88점으로 개선되는 효과가 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제12권1호
• /
• pp.28-35
• /
• 1999

The effects of addition of celulases (A cremonium cellulolyticus and Trichoderma viride, CE), a commercial inoculum containing lactic acid bacteria (Lactobacillus casei, LAB), fermented green juice (macerated napier grass with water was incubated anaerobically with 2% glucose for 1 day, FGJ) and glucose (G), and regional difference of ensiling on napier grass (Pennisetum purpureum Schum.) silage were studied by using 900 ml laboratory glass bottle silos under 30 and $40^{\circ}C$ storage conditions in 1995 and 1996. Experiment 1 was carried out to compare the addition of CE, LAB, FGJ and the combinations. Silages were stored for 45 days after ensiling. Experiment 2 studied the effects of applications of CE, LAB, FGJ and G. Experiment 3 was carried out using the similar additives as experiment 2 except for LAB. Silages were stored for 60 days in the experiments 2 and 3. Experiments 1 and 2 were done in Nagoya, and experiment 3 in Okinawa. Sugar addition through CE or G improved the fermentation quality in all the experiments, which resulted in a greater decrease in the pH value and an increased level of lactic acid, while butyric acid contents increased under $30^{\circ}C$ storage condition in CE addition. LAB and FGJ additions hardly affected the silage fermentation quality without additional fermentable carbohydrate. But the combination of LAB, FGJ and glucidic addition (CE and G) improved the fermentation quality. The effect of the regional difference of ensiling between temperate (Nagoya; $35^{\circ}$ N) and subtropical (Okinawa; $26.5^{\circ}$ N) zones on silage fermentation quality was not shown in the present study.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권2호
• /
• pp.245-253
• /
• 2020

Objective: To develop the fermentation quality and chemical composition of alfalfa (Medicago sativa Lam.) silage, plants were inoculated with different lactic acid bacteria (LAB) strains at field 24 hours before harvest. Methods: The treatment groups were as follow: silage without additive as a control and inoculated with each strains of Lactobacillus brevis (LS-55-2-2), Leuconostoc citerum (L. citerum; L-70-6-1), Lactobacillus bifermentans (L. bifermentans; LS-65-2-1), Lactobacillus plantarum (L. plantarum; LS-3-3) and L. plantarum (LS-72-2). All the silages were stored at 25℃. Parameters such as pH, microorganism and volatile fatty acid contents, crude protein, neutral detergent fiber, acid detergent fiber, net gas, metabolizable energy, organic matter digestibility, dry matter intake and relative feed value were measured to determine fermentation quality, chemical compositions and relative feed value of alfalfa silages. Results: Significant differences were found among the control and treated groups in terms of pH and microorganism contents at all opening times and crude protein, net gas, metabolizable energy and organic matter digestibility of final silage. The pH values ranged from 4.70 to 5.52 for all treatments and control silage had the highest value of overall treatments at T₇₅d silages. Volatile fatty acid of silages was not influenced significantly by inoculations. However, lactic acid content of L. bifermentans (LS-65-2-1) was higher than the other treatments. The highest metabolizable energy and organic matter digestibility were recorded from L. citerum (L-70-6-1) inoculation. In addition, no significant differences were found among treatments in terms of neutral detergent fiber, acid detergent fiber, dry matter intake and relative feed value. Conclusion: Among the treated LAB isolates, L. bifermentans came into prominence especially in terms of organic acid composition and quality characters of silages.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권12호
• /
• pp.1601-1607
• /
• 2010

Two experiments were carried out to investigate i) the effects of four levels of lactic acid bacteria inoculants (LAB; 0, $2{\times}10^5$, $3{\times}10^5$ and $4{\times}10^5$ cfu/g fresh forage) and two physical forms of rice straw (whole and chopped rice straw) on silage fermentation quality and nutritive value of rice straw (RS) silage for lactating Holsteins and ii) the effects of the replacement of corn silage (CS) with different inclusion levels (0, 25 and 50%) of LAB treated RS on lactating performance of Holstein dairy cows. Rice straw packed with stretch film was ensiled for 45 d. The results showed that the higher level of LAB inoculants in the silage quadratically decreased pH, $NH_3$-N and acetic acid concentrations and increased the contents of lactic acid and total organic acids. The CP content and DM losses in the silage declined linearly as the level of LAB addition was increased. Compared with whole-plant rice straw silage (WRS), chopped rice straw silage (CRS) dramatically reduced pH by 0.83. The concentrations of $NH_3$-N were similar in WRS and CRS and both were less than 50 g/kg of total N. Chopping rice straw before ensiling significantly enhanced the lactic acid concentration and total organic acids content whereas the concentration of acetic acid declined. The CP, NDF and ADF content of CRS was 13.4, 5.9 and 10.2% lower than in WRS, respectively. Except for butyric acid concentration, significant interaction effects of inoculation level and physical form of RS were found on all fermentation end-products. Our findings indicated that milk yield and composition were not affected by different level of RS inclusion. However, because of the lower cost of WRS, cows consuming a ration in which WRS was partially substituted for CS had 3.48 Yuan (75% CS+25% WRS) and 4.56 Yuan (50% CS+50% WRS) more economic benefit over those fed a CS-based ration. It was concluded that the chopping process and LAB addition could improve the silage quality, and that substitution of corn silage with RS silage lowered the cost of the dairy cow ration without impairing lactation performance.

• 한국초지조사료학회지
• /
• 제20권4호
• /
• pp.295-302
• /
• 2000

The quality of the rice straw silage added with apple pomace was investigated in this study and the amount of apple pomace added in different treatments were 0, 20, 40 and 60%, respectively. Crude protein contents (6.4-7.5%) of rice straw silage added with apple pomace were significantly (P<0.05) higher than that of 100%. rice straw silage (5.3%), however, crude ash contents were lower (P<0.05) in supplementation of apple pomace. The trends of changing chemical composition between raw materials and end products of silages particularly in the contents of crude protein and crude ash were more apparent in the silages added with apple pomace by 40-60%. Values of pH and the contents of lactic acid and total acid in 40-60% apple pomace added silages were 3.9-4.1, 1.0- 1.5% and 2.7-4.5%, respectively which were significantly (P<0.05) higher than those of 4.6, 0.02% and 0.34% in 100% rice straw silage, respectively. In situ dry matter (DM) and neutral detergent fiber (NDF) disappearance rates in the rumen in the treatments of 40- 60% apple pomace added silages were significantly (P<0.05) higher than those of 100% rice straw silage particularly since after 3 and 24 hour incubation on DM and NDF disappearance, respectively. Although quickly degraded fraction (a) among the treatments were not significantly different, 28.4-28.5% of slowly degraded fraction (b) and 27.2-27.4% of effective degradability (ED, k=0.08) for DM were significantly (P<0.05) higher than those of 100% rice straw silage (12.5 and 24.6% respectively). NDF was in the same trend as in DM. 31.6-63.2% of NDF for b fraction and 18.7- 19.4% for ED in 40-60% apple pomace added silages were significantly (P<0.05) higher than those of 100% rice straw silage (12.4 and 17.6% respectively). (Key words : Rice straw silage, Apple pomace, Lactic acid, In situ digestibility, Effective degradability)

• 한국초지조사료학회지
• /
• 제35권3호
• /
• pp.201-206
• /
• 2015

An experiment was carried out to determine the homofermentative activity of Lactobacillus plantarum KCC-10 and KCC-19 on the ensiling of whole crop barley (WCB). The crude protein in the silages was slightly higher in the KCC-10 and KCC-19 treatments compared to the control, but there was no significant difference between the two inoculant-treated silages. Nutrient parameters such as acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility in L. plantarum KCC-10 and KCC-19 treated silages did not differ from those in the control silage. The lactic acid content increased in KCC-10 and KCC-19 treated silage when compared with the control silage but the contents of acetic acid and butyric acid produced in KCC-10 and KCC-19 treated silages were similar with the control silage. Further, the number of lactic acid bacteria (LAB) in KCC-10 treated silage demonstrated a significant increase when compared to the control. Especially, KCC-19 treated silage showed greater lactic acid bacterial growth potential. Other microbes such as yeast and fungi were not detected in KCC-10 and KCC-19 treated WCB silages. Hence, this study suggests that the addition of L. Plantarum KCC-10 and KCC-19 to the WCB silage can improve fermentation quality for the production of high-quality silage.

• 한국초지조사료학회지
• /
• 제4권1호
• /
• pp.23-27
• /
• 1983

가을 김장철에 일시적으로 생산되는 채소잎으로 품질이 좋은 양질 Silage를 생산하기 위하여 본 시험을 실시 하였으며 시험구를 6개구로 설치하여 시험한 결과를 요약하면 1. 채소잎70%, 볏짚20,% 맥강10% 혼합구가 Silage질이 가장 좋았고 유기산 생성량도 많고 낙산 함량은 없었으며 타구에 비하여 가장 우수하였다. 2. 맥강을 첨가한구는 첨가치 않은 구에 비하여 기호성이 좋았고 조단백질 함량도 높았다. 3. 유기산 조성에의한 Silage품질은 총 산함량에 비하여 낙산 생성이 최하 $0{\sim}120%$ 정도로 나타냈고 반면 양질의 Silage 조제에 필요한 유기산 함량은 $53{\sim}90%$로 양호하였고 PH는 기대 한바 보다 높았다. 4. 외관상으로 볼 때 색깔, 냄새, 촉감 등이 양호한 편이고 기호성도 좋았다. 결론적으로 채소잎은 훌륭한 Silage의 재료이다.

• 한국초지조사료학회지
• /
• 제35권3호
• /
• pp.195-200
• /
• 2015

An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.

• 한국초지조사료학회지
• /
• 제38권1호
• /
• pp.1-6
• /
• 2018

이 연구는 저장기간 및 비닐겹수에 따른 귀리 사일리지의 사료가치 및 사일리지 품질특성을 구명하고자 수행하였다. 귀리 사일리지의 조단백질 함량은 저장기간 모두 사일리지 제조 전에 비해 높아지는 경향을 보였고, 저장기간이 길어질수록 높은 값을 보였지만 통계적인 유의성은 없었다. 비닐겹수에 따라서는 6겹 처리한 처리구가 4겹보다 높은 경향이었다. NDF와 ADF 함량 역시 저장기간 모두 사일리지 제조 전보다 높은 경향을 보였고, 저장기간 및 비닐겹수에 따라서는 비슷하였다. TDN 함량은 저장기간 모두 사일리지 제조 전보다 낮은 경향이었고, 저장기간 및 비닐겹수에 따라서는 비슷한 값을 보였다. pH는 저장기간이 길어짐에 따라 낮아지는 경향을 보였고, 비닐겹수에 따라서는 6겹에서 낮은 값을 보였다. 유기산 함량에서는 저장기간이 길어짐에 따라 젖산함량과 초산함량은 약간 증가하는 경향을 보였고, 낙산함량은 유의적으로 증가하는 경향이었다(p<0.05). 비닐겹수에 따라서는 6겹에서 4겹보다 높은 젖산함량과 낮은 낙산함량을 보였다(p<0.05). 따라서 귀리 사일리지는 4겹으로 처리했을 경우에는 6개월 안에 사용하고, 장기저장을 고려한다면 6겹이상으로 처리하는 것이 바람직하다고 판단된다.

• 한국초지조사료학회지
• /
• 제31권4호
• /
• pp.423-430
• /
• 2011

본 연구는 논에서 재배된 옥수수를 이용하여 AG-BAG 사일리지의 품질에 미치는 영향을 구명하기 위하여 충남 석문 간척지에서 수행되었다. 옥수수 AG-BAG 사일리지의 수분함량은 68~79% 범위를 유지하였고, pH는 3.49-3.65을 유지하였으며 수확기간에 따른 차이는 크지 않았다. 수확기가 늦어짐에 따라 AG-BAG 사일리지의 조단백질 함량은 감소하는 경향을 보였으나 섬유소인 NDF 및 ADF 함량은 증가하였다. 또한 수확기가 늦어짐에 따라 젖산 함량은 증가되었으나(P<0.05) 초산 함량은 감소되었다. 따라서 본 연구에서는 대단위 간척지 논에서 재배된 옥수수 사일리지 제조에 AG-BAG의 이용은 사일리지 발효가 양호하여 품질을 향상시켰을 뿐 만 아니라 옥수수 사일리지 제조에 경제적이며 효율적인 방법의 하나가 될 수 있다는 것을 보여주었다.