• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.3
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• pp.185-192
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• 2000

This experiment was canied out to determine the effect of management practices on the quality of round baled oat silage at experimental field of Grassland and Forage Crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiments are consist of randomized block design with 3 replications. The treatments are 3 wilting dates(0, 2 and 4 days), 3 wrap colors(white, black and green and 3 inoculant(untreated, Inoculant A and Inocuant B). The crude protein(CP) content was increased by prolonged wilting periods, but the effect of wrap color and inoculant were not founded. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content of all silages were not founded significant difference, but in vitro dry matter digestibility of oat silage with inoculant was significantly higher compare with control. Wilting treatment increased the mean silage acidity compare with control and inoculant treatment significantly reduced silage acidity. Wrap color did not influence the silage acidity. Wilting or inoculant treatments increased lactic acid content but, decreased the content of acetic and butyric acid. The quality grade of all silage were grade 3, except inoculant treated silage. Wilting or inoculant decreased silage DM loss, but wrap color did not effect on siage DM loss. The result of this study indicate that wilting for 2-4 days and inoculant will improve the silage fermentation and quality of round baled oat silage. (Key words : Oat, Wilting, Inoculant, Wrap color, Round bale silage)

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.3
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• pp.139-146
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• 2006

This experiment was conducted to determine the effect of microbial inoculant on the quality of round baled grass silage at experimental field of Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three different inoculant (control, inoculant A, B and C). The contents of ether extract(EE), crude protein(CP) and in vitro dry matter digestibility(IVDMD) in inoculant treatment plots were higher than those in control. Silages treated by Inoculant A, Band C had significantly lower acidity than that of control silage (p<0.05). Dry matter(DM) content of control silage was higher than those of inoculant treated silage. There were significant differences in organic acid contents among treatments(p<0.05). Lactic acid was increased with inoculant treatment, but, acetic and butyric acid was decreased. The DM loss of all silages were decreased with inoculant treatment and quality grade of inoculant treated silage was higher than that of control. Results of this study indicate that addition of microbial inoculant will improve the fermentation and quality of round baled grass silage.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.369-378
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• 2011

The effect of a homolactic inoculant containing a blend of Lactobacillus plantarum, Pediococcus acidilactici and Enterococcus faecium or, the anionic surfactant, sodium dodecyl sulphate (SDS), alone or in combination on fermentation characteristics, aerobic stability and in situ DM, OM and NDF degradability of barley silage was investigated. Barley (Hordeum vulgare, L.) was harvested (45% DM), chopped and treated with water at 24 ml/kg forage (Control), inoculant at $1.09{\times}10^5$ cfu/g forage (I), SDS at 0.125% (wt/wt) of forage (S) or with the inoculant ($1.09{\times}10^5$ cfu/g) plus SDS (0.125% wt/wt; I+S). The treated forages were ensiled in triplicate mini silos and opened for chemical and microbiological analyses on d 1, 2, 3, 7, 14, 42 and 77. Silage samples from d 77 were opened and aerobically exposed for 7 d. The in situ rumen degradability characteristics of silage DM, OM and NDF were also determined. The terminal concentration of NDF in S and I+S was lower (p<0.001) than in other treatments. Lactate concentration was higher (p<0.001) and the rate and extent of pH decline were greater (p<0.001) in I and I+S than S and Control silages. A homolactic pathway of fermentation in I and I+S was evidenced by reduced (p<0.001) water-soluble carbohydrates concentration, higher lactate (p<0.01), lower acetate (p<0.01) and lower pH values (p<0.001) than in S and Control silages. All silages remained stable over 7 d of exposure to air as indicated by lower temperatures and moulds, and by non-detectable yeast populations. The treated silages had lower DM and OM degradability than in the Control but NDF degradation characteristics of I+S were improved compared to other treatments. It is concluded that the inoculant alone improved the fermentation characteristics whereas the combination of the inoculant with SDS improved both fermentation and NDF degradability of barley silage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.115-120
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• 1999

This experiment was carried out to investigate the effects of additives on the quality of alfalfa(Medicago sativa L.) silage at the forage experimental field, National Livestock Research Institute, RDA, Suwon in 1996. The experiment was arranged in a randomized block design with three replications. The treatments consisted of different additives(control, formic acid, molasses, inoculant A and inoculant B). Crude protein(CP) and Nitrogen free extract(NFE) content of alfalfa silage with additives were higher compared with those obtained control, but Crude ash(CA) and Crude fiber(CF) content of control(11.5 and 39.6%) were higher than those of additive treated plots. ADF and NDF contents of control silage were the highest as 36.2 and 48.6%, respectively, and increased by 4% compared with those obtained before ensiling. The acidity(pH) of control was the highest(5.45), but the lowest in inoculant A(4.32). Inoculant teratment significantly reduced acidity of silage compared with control. DM content of control silage was the lowest but DM loss of control silage was higher than that of additive treated silage. Ammonia-N content was the highest at formic acid treatment and level of Lactobacillus was decreased at control and formic acid treated silage. Total organic acid and lactic acid content of inoculant A and inoculant B were higher than those of other treated plots. The results of this study indicate that the treatment of LAB(Lactic acid bacteria) could be recommended as producing high quality of alfalfa silage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.4
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• pp.283-289
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• 2015

Three experiments were carried out to determine the effect of harvest maturity and management practices on the dry matter losses and morphological changes of round baled rye silage. Rye was harvested at three growing stages (boot, heading and flowering stage) in three different conditions i) with wilting (unwilted, short wilting and long wilting), ii) with inoculant treatment (untreated, inoculant A and inoculant B) and iii) with three different wrap colors (white, black and green). The morphological changes in round bale silage after 2 months was heavy in the early harvest and unwilted silage. However, harvesting after the heading stage did not change the shape, significantly. Inoculant treatment reduced the morphological changes and dry matter (DM) losses of round baled rye silage. DM loss was decreased with a delayed harvesting date and was significantly reduced by the inoculant. Dramatic changes in the shape were observed in all treatment at boot stage. Inoculant treatment resulted in more severe changes in the boot stage compared to untreated silage. Black color wrapping had the greatest impact among the wrap colors and there were no significant difference in the wrap colors. Harvest maturity and wilting periods was highly correlated (p<0.01) with morphological change. Result of these studies indicated that dry matter content is the most important factor that affects the morphological change in round bale silage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.235-244
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• 2009

This experiment was conducted to develop a new silage inoculant for barley at forage analysis laboratory, Grassland and Forages Division, National Institute of Animal Science, RDA from 2000 to 2002. Barley is very important crop in Korea. The great part of them is utilized as forage. Generally, it contains a lot of grains that are feed of animal, especially whole crop silage in ruminant. Efficient lactic acid bacteria were isolated from good barley silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Four lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be Lactobacillus plantarum on the basis of the biochemical characteristics and utilization of substrates. Barley was ensiled at dough stage following treatment with four lactic acid bacteria, commercial inoculant, and no additive (control). After 2 months, B2-2 bacteria inoculated silage was lower pH and higher lactic acid content than others treatments. The Flieg's score and grade of B2-2 bacteria treated silage were higher than commercial inoculant. According to this experiment, Lactobacillus plantarum B2-2 (NLRI 201) was recommendable for good silage inoculant of whole crop barley silage.

• Animal Bioscience
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• v.36 no.5
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• pp.720-730
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• 2023

Objective: This study investigated the effects of corn silage as a source of microbial inoculant containing antifungal and carboxylesterase-producing bacteria on fermentation, aerobic stability, and nutrient digestibility of fermented total mixed ration (FTMR) with different energy levels. Methods: Corn silage was used as a bacterial source by ensiling for 72 d with an inoculant mixture of Lactobacillus brevis 5M2 and L. buchneri 6M1 at a 1:1 ratio. The corn silage without or with inoculant (CON vs MIX) was mixed with the other ingredients to formulate for low and high energy diets (LOW vs HIGH) for Hanwoo steers. All diets were ensiled into 20 L mini silo (5 kg) for 40 d in quadruplicate. Results: The MIX diets had lower (p<0.05) acid detergent fiber with higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber compared to the CON diets. In terms of fermentation characteristics, the MIX diets had higher (p<0.05) acetate than the CON diets. The MIX diets had extended (p<0.05) lactic acid bacteria growth at 4 to 7 d of aerobic exposure and showed lower (p<0.05) yeast growth at 7 d of aerobic exposure than the CON diets. In terms of rumen fermentation, the MIX diets had higher (p<0.05) total fermentable fraction and total volatile fatty acid, with lower (p<0.05) pH than those of CON diets. The interaction (p = 0.036) between inoculant and diet level was only found in the immediately fermentable fraction, which inoculant was only effective on LOW diets. Conclusion: Application of corn silage with inoculant on FTMR presented an antifungal effect by inhibiting yeast at aerobic exposure and a carboxylesterase effect by improving nutrient digestibility. It also indicated that fermented feedstuffs could be used as microbial source for FTMR. Generally, the interaction between inoculant and diet level had less effect on this FTMR study.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.4
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• pp.333-342
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• 2010

Corn is very important forage in Korea. The great part of them is utilized as silage. Generally, it contains a lot of grains that is feed of animal. This experiment was conducted to evaluation of fermentation ability of microbes for corn silage inoculant. Good lactic acid bacteria were isolated from good corn silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Six lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be lactobacillus plantarum (C3-2, B13-1, CC9-1), Lactobacillus fermentum (C11-4), Lactobacillus paracasei (B14-1), and Leuconostoc lactis (A3-1) on the basis of the biochemical characteristics and utilization of substrates. Corn was ensiled at ripen stage following treatment with selected five lactic acid bacteria, two commercial inoculant, and no additive (control). After 2 month, B13-1 and CC9-1 bacteria inoculated silage were lower pH and higher lactic acid content than others treatments. The Flieg's score and grade of B13-1 and CC9-1 bacteria treated silage were higher than commercial inoculant. According to this experiment, lactobacillus plantarum B13-1 and CC9-1 strain were recommendable for good inoculant of corn silage.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.6
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• pp.949-956
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• 2020

Objective: This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types. Methods: Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 μL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2×10⁵ cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0×10⁵ cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices. Results: Among the wild type inoculants (CON vs AT vs FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen. Conclusion: This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.347-354
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• 1999

This experiment was carried out to determine the effect of maturity at harvest and inoculants on the quality of round baled rye(Secale cereale L.) silage at the experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1998. The experiment was consist of split-plot design with 3 replications. The main plots were 3 harvesting stages such as boot(20 April), heading(29 April), and flowering stages(14 May). The subplots wered inoculant treatments : control (untreated), inoculant A, and inoculant B. Acid detergent fiber(ADF), neutral detergent fiber(NDF), and in vitro dry matter digestibility (IVDMD) of rye silage were significantly increased with delayed harvesting date, but there was not significant difference between inoculants. Mean silage pH at flowering stage was the lowest(4.35), but the highest at early harvest(4.91). Inoculants significantly reduced acidity of silage compared with control. Dry matter(DM) content of the control was higher than that of inoculants. Ammonia-N as proportion of total N was below 10% which was maximum level of high quality silage. The addition of inoculants reduced ammonia-N. There were significant difference in organic acid contents between harvesting stages and inoculants. Lactic acid was increased with inoculants, but acetic and butyric acids were decreased. Various treatments increased colony forming unit(CFU) of lactic acid bacteria by 2 or 3 times compared with the control and the highest at flowering stage with inoculant B treatment. Results of this study indicate that use of microbial inoculant and harvesting after heading stage will improve the silage fermentation and quality of round baled rye silage.