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http://dx.doi.org/10.5713/ajas.19.0308

Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices  

Paradhipta, Dimas Hand Vidya (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Joo, Young Ho (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Lee, Hyuk Jun (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Lee, Seong Shin (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Kwak, Youn Sig (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Han, Ouk Kyu (Department of Crop Science, Korea National College of Agriculture and Fisheries)
Kim, Dong Hyeon (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Kim, Sam Churl (Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.6, 2020 , pp. 949-956 More about this Journal
Abstract
Objective: This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types. Methods: Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 μL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2×105 cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0×105 cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices. Results: Among the wild type inoculants (CON vs AT vs FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen. Conclusion: This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.
Keywords
Antifungal; Esterase; Inoculant; Rye; Silage;
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